Concerns were raised following publication of this article [1,2] regarding the availability of the genetically modified bacterial strains developed in this study.
During editorial follow-up, authors MM, MC, and MA responded that, to the best of their knowledge, the bacterial strains are no longer available.
The PLOS One Editors are also aware that the following patent applications are indexed online with a filing date of November 22, 2012:
- 9657298: Recombinant nitrogen-fixing bacterial strain, inoculum containing the same and application methods
- CN104136599A: Recombinant nitrogen-fixing bacterial strain, inoculum containing the same and application methods
The Competing Interests statement for the article declares that no competing interests exist. MM, MC, and MA stated that at the time of submission and publication of [1], they were not aware of any patent, patent application, or procedure related to the intellectual property protection of the bacterial strains described in [1]. The PLOS One Editors have been unable to contact the remainder of the authors of [1] to clarify the existence of patents, products, or products under development related to the content of [1].
In light of the concerns above regarding the availability of the strains required to reproduce the reported results, and the patent applications that were not declared, the PLOS One Editors issue this Expression of Concern.
References
- 1. Setten L, Soto G, Mozzicafreddo M, Fox AR, Lisi C, Cuccioloni M, et al. Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions. PLoS One. 2013;8(5):e63666. pmid:23675499
- 2. Setten L, Soto G, Mozzicafreddo M, Fox AR, Lisi C, Cuccioloni M, et al. (2013) Correction: Engineering Pseudomonas protegens Pf-5 for Nitrogen Fixation and its Application to Improve Plant Growth under Nitrogen-Deficient Conditions. PLoS ONE 8(10).
Citation: The PLOS One Editors (2026) Expression of concern: Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions. PLoS One 21(6): e0351791. https://doi.org/10.1371/journal.pone.0351791
Published: June 17, 2026
Copyright: © 2026 The PLOS One Editors. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.