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Open Access

Correction

Correction: Generation of Human Melanocytes from Induced Pluripotent Stem Cells

  • Shigeki Ohta,
  • Yoichi Imaizumi,
  • Yohei Okada,
  • Wado Akamatsu,
  • Reiko Kuwahara,
  • Manabu Ohyama,
  • Masayuki Amagai,
  • Yumi Matsuzaki,
  • Shinya Yamanaka,
  • Hideyuki Okano,
  • Yutaka Kawakami

The Fig 4C 3F-iPS EB panel, and the Fig 4D 3F-iPS EB SOX10 and PAX3 panels are incorrect. The authors provided an updated Fig 4 presenting the correct panels and the triplicate image data underlying the Fig 4C and Fig 4D results [S1-S2 File]. The enlarged images in the original Fig 4D 4F-iPS EB SOX10 DAPI and 4F-iPS EB PAX3 DAPI were replaced with clearer images from the same samples. The authors state that the underlying data for Figs 1-3, and Figs 4A-B remain available upon request.

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Fig 4. 3F- and 4F-iPS cells can be differentiated into NCSC and MELSCs.

(A) Gene expression analysis of a neural crest stem cell marker (Slug), a melanocyte stem cell marker (PAX3), a melanocytic marker (MITF), pluripotency marker (NANOG) in 3F-iPS cells, differentiated cells derived from EBs on fibronectin-coated plates in melanocyte differentiation medium at 7 days after differentiation (EB-Diff), and 3F-iPS derived melanocytes (3F-iPS MEL). Transcript levels were normalized to GAPDH. The graphs show the average of two independent experiments. Error bar indicates mean±S.E.M. (B) Representative flow cytometry results for HNK-1 and p75 staining in 3F-iPS EB-derived cells cultured in melanocyte differentiation medium for one week. Dead cells were excluded using PI staining. (C) Immunocytochemistry revealing cells positive for NCSC markers (p75, HNK-1, and AP2α) in both 3F- and 4F-iPS cells derived EB-differentiated cells 7 days after differentiation. Scale bar, 50 µm. (D) Images show cells positive for neural crest cell marker SOX10, as well as possible melanocyte stem cell marker, PAX3 in both 3F- and 4F-iPS cell-derived EB-differentiated cells 7 days after differentiation. Scale bar, 50 µm and 10 µm (insert images).

https://doi.org/10.1371/journal.pone.0337375.g004

Supporting information

S1 File. Triplicate image data underlying the Fig 4C results.

https://doi.org/10.1371/journal.pone.0337375.s001

(ZIP)

S2 File. Triplicate image data underlying the Fig 4D results.

https://doi.org/10.1371/journal.pone.0337375.s002

(ZIP)

Reference

  1. 1. Ohta S, Imaizumi Y, Okada Y, Akamatsu W, Kuwahara R, Ohyama M, et al. Generation of human melanocytes from induced pluripotent stem cells. PLoS One. 2011;6(1):e16182. pmid:21249204

Citation: Ohta S, Imaizumi Y, Okada Y, Akamatsu W, Kuwahara R, Ohyama M, et al. (2025) Correction: Generation of Human Melanocytes from Induced Pluripotent Stem Cells. PLoS One 20(11): e0337375. https://doi.org/10.1371/journal.pone.0337375

Published: November 24, 2025

Copyright: © 2025 Ohta et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.