Dual function of mitochondrial complex III in Plasmodium falciparum

River S. Rell; Anurag Shukla; Joanne M. Morrisey; Ijeoma C. Okoye; Michael W. Mather; Akhil B. Vaidya

doi:10.1371/journal.pone.0334727

Abstract

Complex III of the malaria parasite mitochondrial electron transport chain (mtETC) has been validated as an attractive target for currently used antimalarials. We previously showed that the main function of mtETC in blood stage Plasmodium falciparum is to regenerate ubiquinone, which serves as an obligatory co-substrate of dihydroorotate dehydrogenase (DHOD), an essential mitochondrial enzyme for pyrimidine biosynthesis. P. falciparum can be rendered resistant to all mtETC inhibitors by provision of a bypass mediated by cytosolic yeast DHOD, a fumarate-reducing enzyme. Malaria parasite mitochondrial DNA (mtDNA) encodes only 3 proteins, each a component of mtETC. However, attempts to eliminate mtDNA in transgenic parasites expressing yDHOD have been unsuccessful, suggesting the possibility that essential function(s) other than the canonical redox reactions of the mtETC also require mtDNA maintenance. Here we have tested the hypothesis that Complex III serves the dual functions of processing imported mitochondrial proteins, as well as ubiquinone regeneration. We have generated transgenic lines that conditionally express mitochondrial processing peptidase a (MPPα), which is also a component of Complex III. Using these parasites, we have determined that MPPα is essential even when the need for mitochondrial electron transport is bypassed. MPPα knockdown also resulted in hypersensitivity of the parasites to proguanil, a drug that synergizes with mtETC inhibitors such as atovaquone. Pulldown with MPPα followed by proteomics revealed the association of multiple mitochondrially targeted proteins, in addition to all components of Complex III. These results are consistent with the suggestion that Complex III in P. falciparum serves both mtETC and protein processing functions in mitochondrial physiology.

Citation: Rell RS, Shukla A, Morrisey JM, Okoye IC, Mather MW, Vaidya AB (2026) Dual function of mitochondrial complex III in Plasmodium falciparum. PLoS One 21(2): e0334727. https://doi.org/10.1371/journal.pone.0334727

Editor: Vinoth Rajendran, Pondicherry University, INDIA

Received: October 1, 2025; Accepted: February 5, 2026; Published: February 20, 2026

Copyright: © 2026 Rell et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the manuscript and its supporting information files.

Funding: This work was supported by Grant number R01 AI028398 from National Institutes of Health.The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: No authors have competing interests.

Introduction

Mitochondrial Complex III in Apicomplexan parasites is a clinically validated drug target, and inhibitors of it are in therapeutic use. Divergence of the parasite Complex III from the host underlies the selectivity of these potent drugs [1,2]. Working through a modified Q cycle [3,4], Complex III catalyzes the transfer of electrons from ubiquinol to cytochrome c while transporting protons into the mitochondrial intermembrane space. Inhibition of this step in mtETC results in blockage of all mitochondrial dehydrogenases that require ubiquinone as the electron acceptor. In previous studies, we have shown that in the blood stage P. falciparum the only critical dehydrogenase for parasite survival is dihydroorotate dehydrogenase (DHOD), an essential enzyme that is required for pyrimidine biosynthesis by the parasites [5]. When the parasites are genetically modified to express cytosolic yeast DHOD (yDHOD), they are rendered independent of mtETC, since yDHOD uses fumarate as the electron acceptor, rather than ubiquinone [5]. This metabolic bypass rendered the parasites resistant to all mtETC inhibitors such as atovaquone. Mitochondrial DNA (mtDNA) in malaria parasites is highly reduced in size and encodes only three proteins, each a component of mtETC (cytochrome b and subunits I and III of cytochrome c oxidase) [1,6,7]. Therefore, we reasoned that it should be possible to eliminate mtDNA from mtETC-independent parasites expressing yDHOD. However, our multiple attempts to achieve this by disrupting genes encoding mitochondrial functions required for mtDNA maintenance and mitochondrial translation have failed in transgenic parasites expressing yDHOD [8–10]. Thus, it is likely that the mtETC complexes serve one or more functions in addition to respiration in blood stage malaria parasites. At present the nature of these functions remains unknown.

Except for the 3 proteins encoded by the parasite mtDNA, all mitochondrial proteins are encoded in the nuclear genome, translated in the cytoplasm and imported into the mitochondrion. Many of these proteins contain targeting peptides at their N-termini. These peptides are cleaved off by mitochondrial processing peptidase (MPP) [11,12]. MPPs in mammalian and yeast mitochondria consist of heterodimers of α and β subunits that are localized within the mitochondrial matrix [13,14]. The catalytic sites are present within the β subunit, which is a metalloprotease with a Zn²⁺ ion at the active site [15]. The α subunit does not have catalytic property but is essential for substrate recognition and proper presentation of the peptide within the catalytic site in multiple steps [16,17]. In addition to the matrix-localized MPP in fungi and mammals, Complex III in eukaryotes also contain Core1 and Core2 subunits that have high sequence homology to the MPP subunits α and B, respectively [17–19]. These subunits in yeast have lost their catalytic activity, whereas in mammals they serve to process just one protein, the Rieske 2Fe2S subunit of Complex III [20,21]. Hence, the majority of mitochondrial proteins in these organisms have to be processed by the matrix located MPP. In stark contrast, plant mitochondria do not contain matrix located MPPs, which led Braun to suggest that Complex III associated Core proteins fulfilled MPP function, as later confirmed, and thus Complex III has dual functions in plants: ubiquinol oxidation to serve mtETC and processing of mitochondrially targeted proteins [22,23]. We hypothesize that in malaria parasites correct assembly of Complex III is essential not just for its function in mtETC but also for processing most imported mitochondrial proteins. Thus, even when mtETC has been rendered redundant, Complex III would still need to be assembled to process mitochondrial proteins. In this study we have used conditional expression of MPPα to investigate the potential dual role of Complex III in blood stage P. falciparum.

Materials and methods

P. falciparum culture and transfection

We used P. falciparum D10_VP2KO, in which the pfVP2 locus encoding a type 2 H⁺ pumping pyrophosphatase was disrupted with the yDHOD selectable maker, for transfections [24]. These parasites are resistant to all mtETC inhibitors, as well as inhibitors of parasite DHOD. D10_VP2KO and Dd2attb asexual blood stage parasites were cultured in human (O+) RBC, at 2.5% hematocrit in RPMI1640 medium supplemented with 0.5% Albumax, 2 g/liter NaHCO₃, 15 mM HEPES pH 7.4, 1 mM hypoxanthine and 50 mg/liter gentamicin in 90% N₂, 5% O₂ and 5% CO₂. Testing for Mycoplasma was done on a regular basis to ensure the cultures were free of any such infection.

For transfection, parasite cultures with 5–7% ring stage parasites were washed three times with Cytomix. The parasites were resuspended to 50% hematocrit in Cytomix. 50 μg of linearized pMG75 MPPα plasmid isolated using a Qiagen Maxikit (Qiagen, Valencia, CA, USA) and precipitated in ethanol was centrifuged down and resuspended in 100 μl Cytomix. The plasmid and 250 μl of the parasite cytomix suspension were combined and transferred to a 0.2 cm electroporation cuvette on ice. Electroporations were carried out using a BioRad GenePulser set at 0.31kV, 960uF. The electroporated cells were immediately transferred to a T-25 flask containing 0.2 ml uninfected 50% RBCs and 10 ml medium. Parasites were selected using 2.5 µg/ml Blasticidin and 250 nM anhydrotetracycline (aTc).

Transfection vector generation

Optimal gRNA sequences were identified using CRISPOR software to generate a double strand break within the endogenous MPPα locus [25]. gRNA sequences were cloned into a Cas9-sgRNA vector [26] (S1 A and B).

pMG75 [27] plasmid backbone was used to construct the repair template vector used to help generate D10_VP2KO_MPPα, the MPPα conditional knockdown line in the D10_VP2KO background. [24]. A synthetic gene block was ordered from GenScript containing 584 base pair MPPα 5’ homologous region (nucleotide 1591–2175) in the coding sequence, containing silent blocking mutations in the selected gRNA recognition site, and 550 base pair 3’homologous region (nucleotide 2178–2728) in the 3’UTR, separated by a linker region containing restriction enzyme sites for SacII and BspEII for linearization of the plasmid. The homologous regions are flanked by BstEII and BssHII for ligation into the pMG75 backbone, which contains the TetR-DOZI-aptamer regulatory system, as well as a BSD selectable marker. The synthetic gene was inserted into the pMG75 plasmid backbone via T4 DNA ligase. All plasmids were sequenced using Plasmidsaurus (South San Francisco) nanopore sequencing. The plasmid was linearized with SacII and BspEII before transfection.

Growth assay

Parasites were enriched for late trophozoite and early schizont stages using a layered step gradient of 70%, 60%, and 35% Percoll. Similar amounts of synchronized parasites were split into a 6 well plate containing Human (O+) RBC at 2.5% hematocrit in 10 ml of complete RPMI medium. Triplicate cultures were grown in the presence or absence of aTc, with daily medium change. Cumulative parasitemias were counted over the span of 8 days using flow cytometry after staining with SYBR Green. Cumulative parasitemias were plottedusing GraphPad Prism.

Immunofluorescence assays

50 μL of packed volume parasite cultures were fixed with 4% v/v paraformaldehyde and 0.0075% v/v glutaraldehyde with slow mixing in a rotor for one hour at room temperature. The cells were washed 3x with PBS, followed by permeabilization with 0.125% v/v Triton X-100 in PBS. The cells were treated with NaBH₄ (0.1 mg/ml) for five minutes at room temperature while mixing on a rotor. Samples were washed twice with 1X PBS and then blocked with 3% w/v BSA for 1 hour. Anti-HA (Lot#12923, Santa Cruz Biotechnology) and anti HSP60 (Lot# PP487392 Novus Bio) antibodies were added to the parasites at 1:500 dilution, and the samples were incubated overnight at 4°C in a rotor. Following three PBS washes, Alexa Fluor 488 conjugated goat anti-mouse IgG (Invitrogen Lot# SG251135) and Alexa Flour 568 conjugated goat anti-rabbit (Invitrogen lot #2447870) secondary antibodies were added at 1:250 dilution, followed by overnight incubation at 4°C. After three PBS washes, the treated parasite samples were resuspended in anti-fade buffer (S2828, ThermoFisher Scientific) and imaged using a Nikon Ti microscope.

Live cell microscopy

35 mm glass bottom culture dishes were coated with 0.1% poly-D-lysine overnight and washed 3 times with PBS. 250 μL of 2.5% hematocrit parasite culture was added to the dishes and incubated for 30 min to allow attachment. Culture dishes were washed 3 times with 1XPBS followed by addition of phenol red-free RPMI. Parasites were stained with 62.5 nM MitoTracker Red (Life Technologies by ThermoFisher Scientific) for 20 minutes, followed by 0.5 mg/ml Hoechst nuclear staining for 10 min. Imaging was done using the Nikon Ti microscope. Parasites were temperature controlled at 37 °C. Hoechst-stained parasites were visualized with the DAPI filter set, while MitoTracker red was visualized using the tetramethyl rhodamine isocyanate (TRITC) filter set. Images were 2-dimentionally deconvoluted and processed using Nikon NIS Elements (5.30.02) Imaging Software. The presence of mitochondrial membrane potential or parasite plasma membrane potential was visualized following incubation with MitoTracker red. For statistical analysis of the membrane potential visualization, 30 parasites were counted and imaged. The results were graphed using GraphPad Prism.

Western blotting

Saponin lysed parasite pellets were suspended in approximately ten volumes of 2% w/v SDS, 5% β-mercaptoethanol, and 1% bromophenol blue. Samples were centrifuged for 5 minutes at 13,000g, and the supernatant was subjected to SDS-PAGE. The separated proteins on the gel were transferred to PVDM membranes at 20 volts overnight. Powdered milk solution was used to block the membranes. Blots were then incubated at a dilution ratio of 1:10,000 with primary antibody. After probing with primary antibody (Mouse monoclonal anti-HA Lot#12923, Santa Cruz Biotechnology, Rabbit anti- Plasmodium aldolase Lot#GR289448−4 ABCAM, Rabbit anti HSP60 Lot# PP487392 Novus Bio) blots were washed with Tris-buffered saline with tween (TBST) and incubated with secondary antibody (Goat anti mouse IgG-HRP Lot#GR3219575−4 ABCAM, Mouse anti rabbit IgG-HRP Lot#L1819 Santa Cruz Biotechnology) conjugated with horseradish peroxidase at a concentration of 1:10,000. Blots were developed using Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate (Lot# PB200136).

Immunopulldown and proteomics

Approximately 8x10⁸ parasites were collected as saponin lysed pellets from both wildtype and D10_VP2KO_MPPα and were solubilized overnight at 4^oC in solubilization buffer containing 2% digitonin, 200 mM 6- aminocaproic acid, 50 mM Bis-tris, pH 7, 1 mM EDTA, 1 mM AEBSF, and protease inhibitor cocktail (Sigma-Aldrich). The solubilized sample was centrifuged at 14,000 rpm for 15 min at 4 °C to remove debris, and the supernatant was incubated with anti HA antibody coated magnetic Dynabeads (Pierce Anti-HA, Clone 2–2.2.14, Clone 9E10; Thermo Fisher Scientific). Beads were washed 3 times in 1x PBS, and solubilized total protein was added to 50 μl of the beads, followed by overnight mixing by rotation at 4 °C. Protein-bound beads were washed three times with 1x PBS + 0.02% digitonin. Beads with bound proteins were used for LC/MS on bead proteomics at the University of South Florida Mass Spectrometry & Proteomic Core Facility. Protein samples were prepared for LC-MS/MS using S-Traps (Protifi). 5% SDS in 50 mM triethylammonium bicarbonate (TEAB) was added to the IP beads. Protein samples were reduced with 20 mM dithiothreitol (DTT) at 95 °C for 10 min and alkylated using 40 mM iodoacetamide (IAA) in the dark at room temperature for 30 min. The samples were acidified with 12% phosphoric acid (final concentration 1.2%) before adding 6x volumes of S-trap buffer (90% Methanol, 100 mM TEAB). The protein solution was loaded onto micro S-traps, and centrifuged at 4000xg for 30 sec. Three washes with 150 µl S-trap buffer were performed. 0.5 µg of Trypsin/LysC (Promega) was added to the S-trap filter and digested overnight (~16 hrs) at 37 °C. Peptides were eluted with sequential additions of 50 mM TEAB, water+0.1% formic acid, and 50/50 acetonitrile/water+0.1% formic acid, centrifuged at 4000xg for 1 min each. Peptides were completely dried in a vacuum-centrifuge concentrator before resuspension in water+0.1% formic acid.

Peptides were separated using a Vanquish Neo UHPLC (Thermo) with a 50 cm EASY-Spray PepMap Neo C18 column (Thermo) and analyzed on a Q Exactive Plus hybrid quadrupole mass spectrometer (Thermo). Peptides were separated over a 1hr gradient (Mobile Phase A: water+0.1% Formic Acid, Mobile Phase B: Acetonitrile+0.1% Formic Acid; 2% B-32% B), and data was acquired using data-dependent acquisition (DDA) with a Top 30 method. Full MS scans were acquired at 70k resolution, MS/MS scans were acquired at 17.5k resolution. Raw data files were searched using MaxQuant (v2.4.9) against the current Plasmodium falciparum proteome sequences downloaded from PlasmoDB.org, as well as MaxQuant generated databases of reverse protein sequences and common contaminant protein sequences. Digestion settings specified Trypsin/P specific enzyme digestion, with a maximum of 2 missed cleavages and a minimum peptide length of 7. Both PSM (peptide spectral match) and protein FDR cutoffs were set at 1%. Cysteine carbamidomethylation was set as a fixed modification. Methionine oxidation and acetylation (protein N-term) were set as variable modifications. The MS/MS match mass tolerance was set at 20 ppm. Label Free Quantification intensities of enriched proteins were compared across the replicates to determine fold change and statistical significance using pair wise Student t-test. Volcano plots were visualized using Graphpad Prism.

Growth inhibition assay

All parasite growth inhibition assays were performed in triplicate in 96-well plates as described by Desjardin et al. [28]. P. falciparum-infected erythrocytes had aTc removed 48 hours prior to the start of the assay. At 1.0% initial parasitemia and 1.5% hematocrit, these parasites were exposed to various concentrations of the indicated drug/inhibitor for 48h and then pulsed with 0.5 μCi of ³H-hypoxanthine for an additional 24h. Untreated parasitized and unparasitized red blood cells were incubated concurrently with the treated parasites as controls for growth and radioactive precursor incorporation. The 96 well plates were then frozen at −80°C for 24 h to disrupt erythrocyte and parasite membrane integrity. Following freezing, the plates were warmed to 37°C, and parasites were harvested onto EasyTab^TM-C Self-Aligning Glass Fiber Filters (Packard, Meridian, CT, USA). The filters were then dried completely and placed into an Omnifilter^TM 96-well plate filter case (Packard, Meridian, CT, USA). 30μl of OmniScint^TM (Packard, Meridian, CT, USA) scintillation fluid was added to each well and the radiation was counted with a TopCount^TM counter. The incorporation of radioactivity into nucleic acids served as a measure of cell proliferation and is reported by TopCount as counts per minute (cpm). The total cpm of the non-parasitized red blood cells was subtracted from the cpm of all other wells. The cpm of each sample was converted to percent by dividing it by the cpm of the untreated control. The percent growth versus drug/inhibitor concentration was plotted using GraphPad Prism software. A nonlinear regression dose-response curve was calculated in Prism after adjusting the lowest percent to equal zero and the highest percent to 100.

Results

Generation of parasite lines conditionally expressing MPPα

A parasite line expressing yDHOD (D10_VP2KO) was chosen as the parental line to prepare the MPPα conditional knockdown line. These parasites are independent of mtETC activity and are resistant to all mtETC inhibitors [5,29]. We modified the endogenous MPPα locus (PF3D7_0523100) in D10_VP2KO parasites using CRISPR-Cas9 to generate parasites conditionally expressing 3HA-tagged MPPα under regulation of the aptamer-TetR-DOZI system (S1 Fig). PCR confirmed the integration of the expected portion of the donor plasmid within the modified MPPα locus (Fig 1A and B). Localization of MPPα −3HA was determined by immunofluorescence using anti-HA antibody (Fig 1C). We used HSP-60 antibody as a mitochondrial marker to demonstrate colocalization of MPPα at the parasite mitochondrion, as shown in Fig 1C.

Download:

Fig 1. Generation of parasite lines conditionally expressing MPPα.

(A) Schematic of MPPα locus modification using CRISPR-Cas9. Vertical arrow indicates position of sgRNA binding. Also indicated are the 5’ and 3’ homologous regions (HR), 5’ HR coming from MPPα CDS and the 3’ HR coming from 3’UTR. (B) Using primers indicated by PCR1, amplification of the forward and reverse primers from the endogenous locus shows that the locus is disrupted in the MPPα transfected line. Using primers indicated for PCR2, amplification of the forward primer from the endogenous locus and the reverse primer from the HA tag present in the plasmid determines correct integration of the donor DNA into the parasite genome. (C) Representative immunofluorescence images of a parasite conditionally expressing MPPα, showing its mitochondrial localization.

https://doi.org/10.1371/journal.pone.0334727.g001

MPPα is essential for parasite viability

We assessed expression of MPPα in the presence and absence of aTc by carrying out western blot analysis of parasite lysates collected over 8 days of parasite cultures; HSP60 expression was used as a loading control. As shown in Fig 2A and B, expression of MPPα was below detection level by day 4 of parasite growth in absence of aTc. We assessed the effects of MPPα knockdown on parasite growth by removing aTc at the trophozoite stage of the parasite. As shown in Fig 2C, parasite growth ceased in the second cycle following aTc removal. No viable parasites were detected via Giemsa stain in MPPα (-) condition by day 6 following aTc removal. Since these studies were carried out using a parasite line that was independent of mtETC activity by having the metabolic bypass provided by yDHOD, suggesting that MPPα serves a critical function in addition to supporting mtETC activity as a subunit of complex III.

Download:

Fig 2. MPPα is essential for parasite viability.

(A) Western blot analysis using anti-HA and anti-HSP60 antibodies. (B) Quantification of Western blot normalized to intensities of anti-HSP60. Pixel intensities were measured via Image J. (C) Assessment of growth of MPPα (+) and MPPα(-) parasites. Growth assay data was conducted in triplicate by flow cytometry using SYBR green stained parasites. Graphpad Prism was used for statistical analysis and plotting of parasitemia.

https://doi.org/10.1371/journal.pone.0334727.g002

Mitochondrial membrane potential is disrupted in knockdown parasites

Maintenance of electrochemical potential across the inner mitochondrial membrane is critical for the survival of all eukaryotes. We have previously shown that blood stages of Plasmodium falciparum have two independent means to maintain mitochondrial membrane potential: one dependent on mtETC and the other independent of mtETC [5]. Therefore, we next examined mitochondrial membrane potential in parasites with and without MPPα expression. Using live cell microscopy, the presence of mitochondrial membrane potential was visualized using Mitotracker red staining in both knockdown and wild type parasites. After one cycle of MPPα knockdown, 40% of the parasites were observed to have a diffused Mitotracker staining, which is indicative of collapsed mitochondrial membrane potential. Since parasite plasma membrane potential remains intact (initially), the positively charged probe accumulates within the cytoplasm, but dissipation of the mitochondrial membrane potential would prevent its accumulation in the mitochondrion. After two cycles, 73% of knockdown parasites displayed diffuse staining. By Day 6 of MPPα knockdown, no parasites with mitochondrial staining were visible (Fig 3A and B). While MPPα depletion may directly affect mtETC, the secondary pathway for mitochondrial membrane potential should remain operative. Thus, these data indicate that removal of MPPα affects both the primary and secondary pathways for generating membrane potential.

Download:

Fig 3. Mitochondrial membrane potential is disrupted in knockdown parasites.

(A) Live cell microscopy images of MPPα (+) and MPPα (–) parasites. Mitotracker staining (red) revealed tubular morphology in MPPα(+) parasites but was diffused in varying number of MPPα(-) parasites starting as early as Day 2 following the knockdown (B) Quantitation of morphological profiles of 30 parasites at each time points was assessed, showing gradual increase in parasites showing diffuse Mitotracker staining resulting from disruption of mitochondrial membrane potential.

https://doi.org/10.1371/journal.pone.0334727.g003

Knockdown of MPPα expression causes hypersensitivity to proguanil

A commonly used antimalarial drug, Malarone, is a synergistic combination of atovaquone and proguanil [30,31]. We have previously shown that the synergistic property of proguanil results from its inhibition of the secondary pathway for mitochondrial membrane potential generation [5]. Since MPPα depletion affects both mtETC dependent and independent pathways of generating membrane potential (Fig 4A), we wanted to investigate whether the absence of MPPα influences proguanil sensitivity of the parasites. We carried out growth inhibition assays using ³H-hypoxanthine incorporation by parasites treated with proguanil in presence and absence of MPPα. While MPPα(+) parasites showed EC50 of 1585 nM for proguanil, MPPα(-) parasites were approximately 200-fold hypersensitive to proguanil with EC50 of 8.4 nM (Fig 4B). In contrast, there were no changes in response of the parasites to other inhibitors (DSM1, atovaquone and PA21A092) in presence or absence of MPPα (S3 Fig). We have previously shown that ubiquinone analogue, decyl-ubiquinone, is able to partially rescue parasite growth when subjected to Complex III inhibition [32]. Thus, we assessed the ability of decyl-ubiquinone to reverse hypersensitivity to proguanil in MPPα(-) parasites. Addition of 25 mM decyl-ubiquinone had only a relatively minor effect on hypersensitivity to proguanil in MPPα(-) parasites (Fig 4C).

Download:

Fig 4. Knockdown of MPPα expression causes hypersensitivity to proguanil.

(A) Parasite growth inhibition assessed by ³H-hypoxanthine incorporation following proguanil treatment. For MPPα- parasites, aTc was removed for 48 h followed by ³H-hypoxanthine addition for 72hr. MPPα(+) parasites were also grown for 72hr followed by ³H-hypoxanthine addition. (B) EC₅₀ values for DSM1, atovaquone, PA21A092, and proguanil in MPPα+ and MPPα- parasites. While the EC50 values for Complex III, DHOD and PfATP4 inhibitors were similar for MPPα(+) and MPPα(-) parasites, MPPα(-) parasites were almost 200-fold hypersensitive to proguanil. (C) Parasite growth inhibition assessed by ³H-hypoxanthine incorporation following proguanil treatment and addition of 25 μM decyl-ubiquinone where indicated. For MPPα(-) parasites, aTc was removed for 48 h followed by ³H-hypoxanthine addition for 72hr.

https://doi.org/10.1371/journal.pone.0334727.g004

Proteomic analysis of MPPα immunoprecipitation

To gain insight into interactions of MPPαwith other mitochondrial proteins, we carried out immunoprecipitation from both MPPα tagged and wildtype parasites using anti-HA beads followed by LC-MS/MS analysis. Overall, 93 proteins were identified as significantly enriched in the MPPα immunoprecipitated protein samples, using a cutoff of two-fold change compared to the control with p-value <0.05 (Fig 5A). Of the putative 12 subunits of Complex III, 11 subunits, including mitochondrially encoded cytochrome b, were enriched greater than a million-fold, based on the label free quantification (LFQ) intensity of the peptides observed. QCR13 (Pf3D7_0817800) was the only putative Complex III protein that was absent, likely due to its small size of just 54 amino acids. Remarkably, there were 45 additional predicted or experimentally confirmed mitochondrial proteins that were equally significantly enriched. This suggests their interactions with MPPα are of similar magnitude as MPPα’s association with Complex III. We postulate the presence of these mitochondrial proteins with MPPα being by virtue of their transient association during processing of their mitochondrial targeting peptides. To further assess protein processing, samples were collected from MPPα. + and MPPα – parasites over the course of 8 days. The collected parasite pellets were used for western blot analysis, which was probed using the anti-HSP60 antibody. HSP60 is a nuclearly encoded chaperone protein that is localized to the matrix of the mitochondria, which requires processing via the MPP pathway. As shown in S4 Fig, progressive MPPα depletion over time results in more of the precursor protein band being revealed, indicating a lack of proper protein processing with the removal of MPPα. Interestingly, previously reported knockdowns of cytochrome c, c₁, and c_-2 do not have the same impact on the size of the HSP60 band, suggesting that this change in size is due to a lack of proper processing by MPPα [33]. The significance of additional proteins that were enriched but not predicted to be mitochondrial remains unclear. It is interesting to note that cytochrome c oxidases subunits 1 and 3 were not enriched. This is likely due to these proteins being encoded by mtDNA and not requiring processing of mitochondrial targeting sequences [1,6]. These data suggest that Complex III is engaged in the dual functions of mtETC integrity, as well as processing imported mitochondrial proteins.

Download:

Fig 5. Proteomic analysis of MPPα immunoprecipitation (A) Volcano plot of enriched proteins with a log₂-fold change of label free quantification (LFQ) intensity and p-values of < 0.05 found in immunoprecipitated MPPα samples.

The red dots represent proteins present in Complex III. The purple dots represent proteins that are predicted to be targeted to the mitochondria. The blue dots represent the other proteins that were significantly enriched in the immunoprecipitated samples. (B) Pie chart depicting the number of proteins that fall into the categories of Complex III, likely mitochondrially targeted, and other enriched proteins.

https://doi.org/10.1371/journal.pone.0334727.g005

Discussion

Although mitochondria in all extant organisms originated from the symbiotic arrangement between an archaeon and an α-proteobacterium that led to the emergence of eukaryotes, mitochondrial genomes and physiology are as divergent as the organisms in which they reside [34,35]. Oxidative phosphorylation to generate ATP, while a major function of mitochondria in many organisms, is not essential in some organisms, especially those that live in anaerobic environments [36]. Indeed, such organisms often lack mtDNA but continue to possess a remnant mitochondrion called a mitosome [37,38]. In addition, certain yeasts [39], cultured vertebrate cells [40] and bloodstream-forms of trypanosomes [41] can be manipulated to lose their mtDNA while maintaining the mitochondrion itself. These are called rho⁰ cells, and investigations with such rho⁰ cells have helped to establish that the absolute essential function of their retained mtDNA-negative mitochondria is to synthesize and export inorganic Fe-S clusters for a variety of iron-sulfur proteins required for cell survival [42,43]. To permit this essential function, mitochondria need to maintain an electropotential across their inner membrane, which is required for protein and metabolite transport. In mitochondria with an active mtETC the membrane potential is normally established through proton pumping by the respiratory complexes. A lack of mtDNA will lead to the absence of mtETC, so then the electropotential will need to be generated through alternative means, generally by reverse action of ATP synthase and/or by electrogenic exchange of ATP/ADP [44].

Asexual blood stage P. falciparum rely on glycolysis for ATP generation with little contribution from mitochondrial oxidative phosphorylation [45,46]. Yet the parasite maintains an active mtETC, inhibition of which by antimalarials such as atovaquone results in parasite death. We have previously shown that the critical function of mtETC in blood stage P. falciparum is to re-oxidize ubiquinol to support mitochondrial DHOD, which is needed for pyrimidine biosynthesis, an essential pathway in the parasites [5]. Parasites engineered to bypass mitochondrial DHOD become mtETC independent and resistant to drugs such as atovaquone. We also showed that generation of electropotential across the mitochondrial inner membrane was still required in these mtETC-independent parasites, and that the generation of this potential could be inhibited by proguanil, resulting in parasite death when combined with mtETC inhibitors [5]. Since mtDNA in P. falciparum encodes just three components of mtETC, we initially reasoned that it should be possible generate rho⁰ parasites. However, various attempts failed to generate such parasites [8–10]. It is of interest to note that all organisms where rho⁰ cells can be generated contain matrix-localized MPP (e.g., yeast, trypanosomes, and mammals), whereas organisms with Complex III associated MPP, such as plants (and P. falciparum), cannot be rendered rho⁰. Because cytochrome b of Complex III is always encoded by mtDNA, maintenance of mtDNA remains essential in these organisms for proper assembly of MPP within Complex III, even when mtETC is made non-essential.

A previous study by Espino-Sanchez et al. [33] assessed effects of conditionally knocking down expression of cytochrome c₁, a component of Complex III, in P. falciparum. They showed that the parasites lacking this component of Complex III failed to survive but could be rescued by addition of decyl-ubiquinone, consistent with Complex III being the source of ubiquinone to support DHOD. Furthermore, parasites lacking cytochrome c₁ continued to maintain mitochondrial membrane potential, which could be collapsed by treatment with proguanil [33]. These results are consistent with prior inferences that Complex III is essential for mtETC activity. In contrast to cytochrome c₁ knockdown, however, we show here that knockdown of MPPα not only results in parasite demise but also a collapsed mitochondrial membrane potential. Parasites with MPPα knockdown cannot be rescued by either the presence of yDHOD or addition of decyl-ubiquinone. Since MPPα is a component of Complex III, our study establishes a secondary role of MPP in addition to maintaining the structure and function of Complex III of mtETC. We posit that this secondary role is to process mitochondrially targeted proteins. In support of this, we observed peptides from multiple mitochondrially targeted proteins in proteomic analyses of MPPα pulldown samples (Fig 5). We suggest that these peptides are generated from proteins transiently associated with Complex III as they are being processed by the MPP subunits.

Supporting information

S1 Fig. Maps of plasmids used for transfections.

Maps of the two gRNA plasmids (A) and (B), and the map for double cross over modification of MPPα locus (C) are shown.

https://doi.org/10.1371/journal.pone.0334727.s001

(TIFF)

S2 Fig. MPPα disruption in a second parasite line not expressing yDHOD.

(A) Assessment of growth of MPPα + and MPPα - parasites in Dd2attb parasites. Growth assay was conducted in triplicate by counting 500 parasites via Giemsa stain. (B) Western blot analysis using anti-HA and anti-Aldolase antibodies.

https://doi.org/10.1371/journal.pone.0334727.s002

(TIFF)

S3 Fig. Susceptibility of MPPα - parasites to antimalarials remains unchanged.

Parasite growth inhibition assessed by ³H-hypoxanthine incorporation following DSM1, atovaquone, PA21A092 treatment. For MPPα - parasites, aTc was removed for 48 h followed by 3H-hypoxanthine addition for 72 hr.

https://doi.org/10.1371/journal.pone.0334727.s003

(TIFF)

S4 Fig. Proteomic analysis of MPPα immunoprecipitation.

Western blot analysis of MPPα+ and MPPα- parasites using anti-HSP60 and anti-Aldolase antibodies. As MPPαdepletion progresses, more of the unprocessed HSP60 band is present in the Western Blot analysis.

https://doi.org/10.1371/journal.pone.0334727.s004

(TIF)

S1 File. Primers and DNA sequences.

Excel file indicating all the primers and DNA sequences that were synthesized and used to generate the MPPαconditional knockdown parasite line.

https://doi.org/10.1371/journal.pone.0334727.s005

(XLSX)

S2 File. Raw Images.

Uncropped and unchanged images that correspond to the cropped western blots found in Fig 2 and S2 of the manuscript. Each blot is labeled with the antibody that was used to probe the membrane, the size of the band detected, and each sample is labeled at the lane of the gel. Lanes with an X were not used. Each blot also indicates which parasite line was used for western blot analysis.

https://doi.org/10.1371/journal.pone.0334727.s006

(TIFF)

S1 Data. Dataset.

Excel file including all unanalyzed mass spec data from the proteomics results that are included in Fig 5. This file also includes the analysis of the mass spec data, including how significance was calculated and displays the selected proteins compared to the proteins below the significance threshold. All calculations and data interpretation are included in this spreadsheet.

https://doi.org/10.1371/journal.pone.0334727.s007

(XLSX)

Acknowledgments

We thank Dr. Dale Chaput at University of South Florida Advanced Research Core for Mass Spectrometry for assistance with proteomic analysis.

References

1. Vaidya AB, Lashgari MS, Pologe LG, Morrisey J. Structural features of Plasmodium cytochrome b that may underlie susceptibility to 8-aminoquinolines and hydroxynaphthoquinones. Mol Biochem Parasitol. 1993;58(1):33–42. pmid:8459834
- View Article
- PubMed/NCBI
- Google Scholar
2. Fry M, Pudney M. Site of action of the antimalarial hydroxynaphthoquinone, 2-[trans-4-(4’-chlorophenyl) cyclohexyl]-3-hydroxy-1,4-naphthoquinone (566C80). Biochem Pharmacol. 1992;43(7):1545–53.
- View Article
- Google Scholar
3. Crofts AR. The Q-cycle - A Personal Perspective. Photosynth Res. 2004;80(1–3):223–43. pmid:16328823
- View Article
- PubMed/NCBI
- Google Scholar
4. Mitchell P. The protonmotive Q cycle: A general formulation. FEBS Lett. 1975;59(2):137–9. pmid:1227927
- View Article
- PubMed/NCBI
- Google Scholar
5. Painter HJ, Morrisey JM, Mather MW, Vaidya AB. Specific role of mitochondrial electron transport in blood-stage Plasmodium falciparum. Nature. 2007;446(7131):88–91. pmid:17330044
- View Article
- PubMed/NCBI
- Google Scholar
6. Vaidya AB, Akella R, Suplick K. Sequences similar to genes for two mitochondrial proteins and portions of ribosomal RNA in tandemly arrayed 6-kilobase-pair DNA of a malarial parasite. Mol Biochem Parasitol. 1989;35(2):97–107. pmid:2549417
- View Article
- PubMed/NCBI
- Google Scholar
7. Lamb IM, Okoye IC, Mather MW, Vaidya AB. Unique Properties of Apicomplexan Mitochondria. Annu Rev Microbiol. 2023;77:541–60. pmid:37406344
- View Article
- PubMed/NCBI
- Google Scholar
8. Ke H, Morrisey JM, Ganesan SM, Mather MW, Vaidya AB. Mitochondrial RNA polymerase is an essential enzyme in erythrocytic stages of Plasmodium falciparum. Mol Biochem Parasitol. 2012;185(1):48–51. pmid:22640832
- View Article
- PubMed/NCBI
- Google Scholar
9. Ke H, Dass S, Morrisey JM, Mather MW, Vaidya AB. The mitochondrial ribosomal protein L13 is critical for the structural and functional integrity of the mitochondrion in Plasmodium falciparum. J Biol Chem. 2018;293(21):8128–37. pmid:29626096
- View Article
- PubMed/NCBI
- Google Scholar
10. Dass S, Mather MW, Morrisey JM, Ling L, Vaidya AB, Ke H. Transcriptional changes in Plasmodium falciparum upon conditional knock down of mitochondrial ribosomal proteins RSM22 and L23. PLoS One. 2022;17(10):e0274993. pmid:36201550
- View Article
- PubMed/NCBI
- Google Scholar
11. Chacinska A, Koehler CM, Milenkovic D, Lithgow T, Pfanner N. Importing mitochondrial proteins: Machineries and mechanisms. Cell. 2009;138(4):628–44. pmid:19703392
- View Article
- PubMed/NCBI
- Google Scholar
12. Wiedemann N, Pfanner N. Mitochondrial machineries for protein import and assembly. Annu Rev Biochem. 2017;86:685–714. pmid:28301740
- View Article
- PubMed/NCBI
- Google Scholar
13. Pollock RA, Hartl FU, Cheng MY, Ostermann J, Horwich A, Neupert W. The processing peptidase of yeast mitochondria: The two co-operating components MPP and PEP are structurally related. EMBO J. 1988;7(11):3493–500. pmid:3061797
- View Article
- PubMed/NCBI
- Google Scholar
14. Böhni PC, Daum G, Schatz G. Import of proteins into mitochondria. Partial purification of a matrix-located protease involved in cleavage of mitochondrial precursor polypeptides. J Biol Chem. 1983;258(8):4937–43. pmid:6300104
- View Article
- PubMed/NCBI
- Google Scholar
15. Taylor AB, Smith BS, Kitada S, Kojima K, Miyaura H, Otwinowski Z, et al. Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences. Structure. 2001;9(7):615–25. pmid:11470436
- View Article
- PubMed/NCBI
- Google Scholar
16. Dvoráková-Holá K, Matusková A, Kubala M, Otyepka M, Kucera T, Vecer J, et al. Glycine-rich loop of mitochondrial processing peptidase alpha-subunit is responsible for substrate recognition by a mechanism analogous to mitochondrial receptor Tom20. J Mol Biol. 2010;396(5):1197–210. pmid:20053354
- View Article
- PubMed/NCBI
- Google Scholar
17. Nagao Y, Kitada S, Kojima K, Toh H, Kuhara S, Ogishima T, et al. Glycine-rich region of mitochondrial processing peptidase alpha-subunit is essential for binding and cleavage of the precursor proteins. J Biol Chem. 2000;275(44):34552–6. pmid:10942759
- View Article
- PubMed/NCBI
- Google Scholar
18. Iwata S, Lee JW, Okada K, Lee JK, Iwata M, Rasmussen B, et al. Complete structure of the 11-subunit bovine mitochondrial cytochrome bc1 complex. Science. 1998;281(5373):64–71. pmid:9651245
- View Article
- PubMed/NCBI
- Google Scholar
19. Berry EA, Guergova-Kuras M, Huang LS, Crofts AR. Structure and function of cytochrome bc complexes. Annu Rev Biochem. 2000;69:1005–75. pmid:10966481
- View Article
- PubMed/NCBI
- Google Scholar
20. Brandt U, Yu L, Yu CA, Trumpower BL. The mitochondrial targeting presequence of the Rieske iron-sulfur protein is processed in a single step after insertion into the cytochrome bc1 complex in mammals and retained as a subunit in the complex. J Biol Chem. 1993;268(12):8387–90. pmid:8386158
- View Article
- PubMed/NCBI
- Google Scholar
21. Yang M, Jensen RE, Yaffe MP, Oppliger W, Schatz G. Import of proteins into yeast mitochondria: The purified matrix processing protease contains two subunits which are encoded by the nuclear MAS1 and MAS2 genes. EMBO J. 1988;7(12):3857–62. pmid:2905264
- View Article
- PubMed/NCBI
- Google Scholar
22. Brumme S, Kruft V, Schmitz UK, Braun HP. New insights into the co-evolution of cytochrome c reductase and the mitochondrial processing peptidase. J Biol Chem. 1998;273(21):13143–9. pmid:9582354
- View Article
- PubMed/NCBI
- Google Scholar
23. Braun H-P. The two roles of complex III in plants. Elife. 2021;10:e65239. pmid:33463522
- View Article
- PubMed/NCBI
- Google Scholar
24. Ganesan SM, Morrisey JM, Ke H, Painter HJ, Laroiya K, Phillips MA, et al. Yeast dihydroorotate dehydrogenase as a new selectable marker for Plasmodium falciparum transfection. Mol Biochem Parasitol. 2011;177(1):29–34. pmid:21251930
- View Article
- PubMed/NCBI
- Google Scholar
25. Concordet J-P, Haeussler M. CRISPOR: Intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens. Nucleic Acids Res. 2018;46(W1):W242–5. pmid:29762716
- View Article
- PubMed/NCBI
- Google Scholar
26. Spillman NJ, Beck JR, Ganesan SM, Niles JC, Goldberg DE. The chaperonin TRiC forms an oligomeric complex in the malaria parasite cytosol. Cell Microbiol. 2017;19(6):10.1111/cmi.12719. pmid:28067475
- View Article
- PubMed/NCBI
- Google Scholar
27. Ganesan SM, Falla A, Goldfless SJ, Nasamu AS, Niles JC. Synthetic RNA-protein modules integrated with native translation mechanisms to control gene expression in malaria parasites. Nat Commun. 2016;7:10727. pmid:26925876
- View Article
- PubMed/NCBI
- Google Scholar
28. Desjardins RE, Canfield CJ, Haynes JD, Chulay JD. Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique. Antimicrob Agents Chemother. 1979;16(6):710–8. pmid:394674
- View Article
- PubMed/NCBI
- Google Scholar
29. Ganesan SM, Morrisey JM, Ke H, Painter HJ, Laroiya K, Phillips MA. Yeast dihydroorotate dehydrogenase as a new selectable marker for Plasmodium falciparum transfection. Mol Biochem Parasitol. 2011.
- View Article
- Google Scholar
30. Looareesuwan S, Chulay JD, Canfield CJ, Hutchinson DB. Malarone (atovaquone and proguanil hydrochloride): a review of its clinical development for treatment of malaria. Malarone Clinical Trials Study Group. Am J Trop Med Hyg. 1999;60(4):533–41. pmid:10348225
- View Article
- PubMed/NCBI
- Google Scholar
31. Srivastava IK, Vaidya AB. A mechanism for the synergistic antimalarial action of atovaquone and proguanil. Antimicrob Agents Chemother. 1999;43(6):1334–9. pmid:10348748
- View Article
- PubMed/NCBI
- Google Scholar
32. Ke H, Morrisey JM, Ganesan SM, Painter HJ, Mather MW, Vaidya AB. Variation among Plasmodium falciparum strains in their reliance on mitochondrial electron transport chain function. Eukaryot Cell. 2011;10(8):1053–61. pmid:21685321
- View Article
- PubMed/NCBI
- Google Scholar
33. Espino-Sanchez TJ, Wienkers H, Marvin RG, Nalder S-A, García-Guerrero AE, VanNatta PE, et al. Direct tests of cytochrome c and c1 functions in the electron transport chain of malaria parasites. Proc Natl Acad Sci U S A. 2023;120(19):e2301047120. pmid:37126705
- View Article
- PubMed/NCBI
- Google Scholar
34. Lane N, Martin W. The energetics of genome complexity. Nature. 2010;467(7318):929–34. pmid:20962839
- View Article
- PubMed/NCBI
- Google Scholar
35. Martin W, Müller M. The hydrogen hypothesis for the first eukaryote. Nature. 1998;392(6671):37–41. pmid:9510246
- View Article
- PubMed/NCBI
- Google Scholar
36. Stairs CW, Leger MM, Roger AJ. Diversity and origins of anaerobic metabolism in mitochondria and related organelles. Philos Trans R Soc Lond B Biol Sci. 2015;370(1678):20140326. pmid:26323757
- View Article
- PubMed/NCBI
- Google Scholar
37. Tovar J, León-Avila G, Sánchez LB, Sutak R, Tachezy J, van der Giezen M, et al. Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation. Nature. 2003;426(6963):172–6. pmid:14614504
- View Article
- PubMed/NCBI
- Google Scholar
38. Shiflett AM, Johnson PJ. Mitochondrion-related organelles in eukaryotic protists. Annu Rev Microbiol. 2010;64:409–29. pmid:20528687
- View Article
- PubMed/NCBI
- Google Scholar
39. Tzagoloff A, Myers AM. Genetics of mitochondrial biogenesis. Annu Rev Biochem. 1986;55:249–85. pmid:2427014
- View Article
- PubMed/NCBI
- Google Scholar
40. King MP, Attardi G. Human cells lacking mtDNA: Repopulation with exogenous mitochondria by complementation. Science. 1989;246(4929):500–3. pmid:2814477
- View Article
- PubMed/NCBI
- Google Scholar
41. Schnaufer A, Clark-Walker GD, Steinberg AG, Stuart K. The F1-ATP synthase complex in bloodstream stage trypanosomes has an unusual and essential function. EMBO J. 2005;24(23):4029–40. pmid:16270030
- View Article
- PubMed/NCBI
- Google Scholar
42. Lill R, Kispal G. Maturation of cellular Fe-S proteins: An essential function of mitochondria. Trends Biochem Sci. 2000;25(8):352–6. pmid:10916152
- View Article
- PubMed/NCBI
- Google Scholar
43. Stehling O, Elsässer H-P, Brückel B, Mühlenhoff U, Lill R. Iron-sulfur protein maturation in human cells: Evidence for a function of frataxin. Hum Mol Genet. 2004;13(23):3007–15. pmid:15509595
- View Article
- PubMed/NCBI
- Google Scholar
44. Schnaufer A, Domingo GJ, Stuart K. Natural and induced dyskinetoplastic trypanosomatids: How to live without mitochondrial DNA. Int J Parasitol. 2002;32(9):1071–84. pmid:12117490
- View Article
- PubMed/NCBI
- Google Scholar
45. Sherman IW. Biochemistry of Plasmodium (malarial parasites). Microbiol Rev. 1979;43(4):453–95. pmid:94424
- View Article
- PubMed/NCBI
- Google Scholar
46. Vaidya AB. Mitochondrial physiology as a target for atovaquone and other antimalarials. Sherman IW. Malaria: Parasite biology, pathogenesis, and protection. Washington, DC: ASM Press; 1998. 355–68.

[ref1] 1. Vaidya AB, Lashgari MS, Pologe LG, Morrisey J. Structural features of Plasmodium cytochrome b that may underlie susceptibility to 8-aminoquinolines and hydroxynaphthoquinones. Mol Biochem Parasitol. 1993;58(1):33–42. pmid:8459834
View Article
PubMed/NCBI
Google Scholar

[2] View Article

[3] PubMed/NCBI

[4] Google Scholar

[ref2] 2. Fry M, Pudney M. Site of action of the antimalarial hydroxynaphthoquinone, 2-[trans-4-(4’-chlorophenyl) cyclohexyl]-3-hydroxy-1,4-naphthoquinone (566C80). Biochem Pharmacol. 1992;43(7):1545–53.
View Article
Google Scholar

[6] View Article

[7] Google Scholar

[ref3] 3. Crofts AR. The Q-cycle - A Personal Perspective. Photosynth Res. 2004;80(1–3):223–43. pmid:16328823
View Article
PubMed/NCBI
Google Scholar

[9] View Article

[10] PubMed/NCBI

[11] Google Scholar

[ref4] 4. Mitchell P. The protonmotive Q cycle: A general formulation. FEBS Lett. 1975;59(2):137–9. pmid:1227927
View Article
PubMed/NCBI
Google Scholar

[13] View Article

[14] PubMed/NCBI

[15] Google Scholar

[ref5] 5. Painter HJ, Morrisey JM, Mather MW, Vaidya AB. Specific role of mitochondrial electron transport in blood-stage Plasmodium falciparum. Nature. 2007;446(7131):88–91. pmid:17330044
View Article
PubMed/NCBI
Google Scholar

[17] View Article

[18] PubMed/NCBI

[19] Google Scholar

[ref6] 6. Vaidya AB, Akella R, Suplick K. Sequences similar to genes for two mitochondrial proteins and portions of ribosomal RNA in tandemly arrayed 6-kilobase-pair DNA of a malarial parasite. Mol Biochem Parasitol. 1989;35(2):97–107. pmid:2549417
View Article
PubMed/NCBI
Google Scholar

[21] View Article

[22] PubMed/NCBI

[23] Google Scholar

[ref7] 7. Lamb IM, Okoye IC, Mather MW, Vaidya AB. Unique Properties of Apicomplexan Mitochondria. Annu Rev Microbiol. 2023;77:541–60. pmid:37406344
View Article
PubMed/NCBI
Google Scholar

[25] View Article

[26] PubMed/NCBI

[27] Google Scholar

[ref8] 8. Ke H, Morrisey JM, Ganesan SM, Mather MW, Vaidya AB. Mitochondrial RNA polymerase is an essential enzyme in erythrocytic stages of Plasmodium falciparum. Mol Biochem Parasitol. 2012;185(1):48–51. pmid:22640832
View Article
PubMed/NCBI
Google Scholar

[29] View Article

[30] PubMed/NCBI

[31] Google Scholar

[ref9] 9. Ke H, Dass S, Morrisey JM, Mather MW, Vaidya AB. The mitochondrial ribosomal protein L13 is critical for the structural and functional integrity of the mitochondrion in Plasmodium falciparum. J Biol Chem. 2018;293(21):8128–37. pmid:29626096
View Article
PubMed/NCBI
Google Scholar

[33] View Article

[34] PubMed/NCBI

[35] Google Scholar

[ref10] 10. Dass S, Mather MW, Morrisey JM, Ling L, Vaidya AB, Ke H. Transcriptional changes in Plasmodium falciparum upon conditional knock down of mitochondrial ribosomal proteins RSM22 and L23. PLoS One. 2022;17(10):e0274993. pmid:36201550
View Article
PubMed/NCBI
Google Scholar

[37] View Article

[38] PubMed/NCBI

[39] Google Scholar

[ref11] 11. Chacinska A, Koehler CM, Milenkovic D, Lithgow T, Pfanner N. Importing mitochondrial proteins: Machineries and mechanisms. Cell. 2009;138(4):628–44. pmid:19703392
View Article
PubMed/NCBI
Google Scholar

[41] View Article

[42] PubMed/NCBI

[43] Google Scholar

[ref12] 12. Wiedemann N, Pfanner N. Mitochondrial machineries for protein import and assembly. Annu Rev Biochem. 2017;86:685–714. pmid:28301740
View Article
PubMed/NCBI
Google Scholar

[45] View Article

[46] PubMed/NCBI

[47] Google Scholar

[ref13] 13. Pollock RA, Hartl FU, Cheng MY, Ostermann J, Horwich A, Neupert W. The processing peptidase of yeast mitochondria: The two co-operating components MPP and PEP are structurally related. EMBO J. 1988;7(11):3493–500. pmid:3061797
View Article
PubMed/NCBI
Google Scholar

[49] View Article

[50] PubMed/NCBI

[51] Google Scholar

[ref14] 14. Böhni PC, Daum G, Schatz G. Import of proteins into mitochondria. Partial purification of a matrix-located protease involved in cleavage of mitochondrial precursor polypeptides. J Biol Chem. 1983;258(8):4937–43. pmid:6300104
View Article
PubMed/NCBI
Google Scholar

[53] View Article

[54] PubMed/NCBI

[55] Google Scholar

[ref15] 15. Taylor AB, Smith BS, Kitada S, Kojima K, Miyaura H, Otwinowski Z, et al. Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences. Structure. 2001;9(7):615–25. pmid:11470436
View Article
PubMed/NCBI
Google Scholar

[57] View Article

[58] PubMed/NCBI

[59] Google Scholar

[ref16] 16. Dvoráková-Holá K, Matusková A, Kubala M, Otyepka M, Kucera T, Vecer J, et al. Glycine-rich loop of mitochondrial processing peptidase alpha-subunit is responsible for substrate recognition by a mechanism analogous to mitochondrial receptor Tom20. J Mol Biol. 2010;396(5):1197–210. pmid:20053354
View Article
PubMed/NCBI
Google Scholar

[61] View Article

[62] PubMed/NCBI

[63] Google Scholar

[ref17] 17. Nagao Y, Kitada S, Kojima K, Toh H, Kuhara S, Ogishima T, et al. Glycine-rich region of mitochondrial processing peptidase alpha-subunit is essential for binding and cleavage of the precursor proteins. J Biol Chem. 2000;275(44):34552–6. pmid:10942759
View Article
PubMed/NCBI
Google Scholar

[65] View Article

[66] PubMed/NCBI

[67] Google Scholar

[ref18] 18. Iwata S, Lee JW, Okada K, Lee JK, Iwata M, Rasmussen B, et al. Complete structure of the 11-subunit bovine mitochondrial cytochrome bc1 complex. Science. 1998;281(5373):64–71. pmid:9651245
View Article
PubMed/NCBI
Google Scholar

[69] View Article

[70] PubMed/NCBI

[71] Google Scholar

[ref19] 19. Berry EA, Guergova-Kuras M, Huang LS, Crofts AR. Structure and function of cytochrome bc complexes. Annu Rev Biochem. 2000;69:1005–75. pmid:10966481
View Article
PubMed/NCBI
Google Scholar

[73] View Article

[74] PubMed/NCBI

[75] Google Scholar

[ref20] 20. Brandt U, Yu L, Yu CA, Trumpower BL. The mitochondrial targeting presequence of the Rieske iron-sulfur protein is processed in a single step after insertion into the cytochrome bc1 complex in mammals and retained as a subunit in the complex. J Biol Chem. 1993;268(12):8387–90. pmid:8386158
View Article
PubMed/NCBI
Google Scholar

[77] View Article

[78] PubMed/NCBI

[79] Google Scholar

[ref21] 21. Yang M, Jensen RE, Yaffe MP, Oppliger W, Schatz G. Import of proteins into yeast mitochondria: The purified matrix processing protease contains two subunits which are encoded by the nuclear MAS1 and MAS2 genes. EMBO J. 1988;7(12):3857–62. pmid:2905264
View Article
PubMed/NCBI
Google Scholar

[81] View Article

[82] PubMed/NCBI

[83] Google Scholar

[ref22] 22. Brumme S, Kruft V, Schmitz UK, Braun HP. New insights into the co-evolution of cytochrome c reductase and the mitochondrial processing peptidase. J Biol Chem. 1998;273(21):13143–9. pmid:9582354
View Article
PubMed/NCBI
Google Scholar

[85] View Article

[86] PubMed/NCBI

[87] Google Scholar

[ref23] 23. Braun H-P. The two roles of complex III in plants. Elife. 2021;10:e65239. pmid:33463522
View Article
PubMed/NCBI
Google Scholar

[89] View Article

[90] PubMed/NCBI

[91] Google Scholar

[ref24] 24. Ganesan SM, Morrisey JM, Ke H, Painter HJ, Laroiya K, Phillips MA, et al. Yeast dihydroorotate dehydrogenase as a new selectable marker for Plasmodium falciparum transfection. Mol Biochem Parasitol. 2011;177(1):29–34. pmid:21251930
View Article
PubMed/NCBI
Google Scholar

[93] View Article

[94] PubMed/NCBI

[95] Google Scholar

[ref25] 25. Concordet J-P, Haeussler M. CRISPOR: Intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens. Nucleic Acids Res. 2018;46(W1):W242–5. pmid:29762716
View Article
PubMed/NCBI
Google Scholar

[97] View Article

[98] PubMed/NCBI

[99] Google Scholar

[ref26] 26. Spillman NJ, Beck JR, Ganesan SM, Niles JC, Goldberg DE. The chaperonin TRiC forms an oligomeric complex in the malaria parasite cytosol. Cell Microbiol. 2017;19(6):10.1111/cmi.12719. pmid:28067475
View Article
PubMed/NCBI
Google Scholar

[101] View Article

[102] PubMed/NCBI

[103] Google Scholar

[ref27] 27. Ganesan SM, Falla A, Goldfless SJ, Nasamu AS, Niles JC. Synthetic RNA-protein modules integrated with native translation mechanisms to control gene expression in malaria parasites. Nat Commun. 2016;7:10727. pmid:26925876
View Article
PubMed/NCBI
Google Scholar

[105] View Article

[106] PubMed/NCBI

[107] Google Scholar

[ref28] 28. Desjardins RE, Canfield CJ, Haynes JD, Chulay JD. Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique. Antimicrob Agents Chemother. 1979;16(6):710–8. pmid:394674
View Article
PubMed/NCBI
Google Scholar

[109] View Article

[110] PubMed/NCBI

[111] Google Scholar

[ref29] 29. Ganesan SM, Morrisey JM, Ke H, Painter HJ, Laroiya K, Phillips MA. Yeast dihydroorotate dehydrogenase as a new selectable marker for Plasmodium falciparum transfection. Mol Biochem Parasitol. 2011.
View Article
Google Scholar

[113] View Article

[114] Google Scholar

[ref30] 30. Looareesuwan S, Chulay JD, Canfield CJ, Hutchinson DB. Malarone (atovaquone and proguanil hydrochloride): a review of its clinical development for treatment of malaria. Malarone Clinical Trials Study Group. Am J Trop Med Hyg. 1999;60(4):533–41. pmid:10348225
View Article
PubMed/NCBI
Google Scholar

[116] View Article

[117] PubMed/NCBI

[118] Google Scholar

[ref31] 31. Srivastava IK, Vaidya AB. A mechanism for the synergistic antimalarial action of atovaquone and proguanil. Antimicrob Agents Chemother. 1999;43(6):1334–9. pmid:10348748
View Article
PubMed/NCBI
Google Scholar

[120] View Article

[121] PubMed/NCBI

[122] Google Scholar

[ref32] 32. Ke H, Morrisey JM, Ganesan SM, Painter HJ, Mather MW, Vaidya AB. Variation among Plasmodium falciparum strains in their reliance on mitochondrial electron transport chain function. Eukaryot Cell. 2011;10(8):1053–61. pmid:21685321
View Article
PubMed/NCBI
Google Scholar

[124] View Article

[125] PubMed/NCBI

[126] Google Scholar

[ref33] 33. Espino-Sanchez TJ, Wienkers H, Marvin RG, Nalder S-A, García-Guerrero AE, VanNatta PE, et al. Direct tests of cytochrome c and c1 functions in the electron transport chain of malaria parasites. Proc Natl Acad Sci U S A. 2023;120(19):e2301047120. pmid:37126705
View Article
PubMed/NCBI
Google Scholar

[128] View Article

[129] PubMed/NCBI

[130] Google Scholar

[ref34] 34. Lane N, Martin W. The energetics of genome complexity. Nature. 2010;467(7318):929–34. pmid:20962839
View Article
PubMed/NCBI
Google Scholar

[132] View Article

[133] PubMed/NCBI

[134] Google Scholar

[ref35] 35. Martin W, Müller M. The hydrogen hypothesis for the first eukaryote. Nature. 1998;392(6671):37–41. pmid:9510246
View Article
PubMed/NCBI
Google Scholar

[136] View Article

[137] PubMed/NCBI

[138] Google Scholar

[ref36] 36. Stairs CW, Leger MM, Roger AJ. Diversity and origins of anaerobic metabolism in mitochondria and related organelles. Philos Trans R Soc Lond B Biol Sci. 2015;370(1678):20140326. pmid:26323757
View Article
PubMed/NCBI
Google Scholar

[140] View Article

[141] PubMed/NCBI

[142] Google Scholar

[ref37] 37. Tovar J, León-Avila G, Sánchez LB, Sutak R, Tachezy J, van der Giezen M, et al. Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation. Nature. 2003;426(6963):172–6. pmid:14614504
View Article
PubMed/NCBI
Google Scholar

[144] View Article

[145] PubMed/NCBI

[146] Google Scholar

[ref38] 38. Shiflett AM, Johnson PJ. Mitochondrion-related organelles in eukaryotic protists. Annu Rev Microbiol. 2010;64:409–29. pmid:20528687
View Article
PubMed/NCBI
Google Scholar

[148] View Article

[149] PubMed/NCBI

[150] Google Scholar

[ref39] 39. Tzagoloff A, Myers AM. Genetics of mitochondrial biogenesis. Annu Rev Biochem. 1986;55:249–85. pmid:2427014
View Article
PubMed/NCBI
Google Scholar

[152] View Article

[153] PubMed/NCBI

[154] Google Scholar

[ref40] 40. King MP, Attardi G. Human cells lacking mtDNA: Repopulation with exogenous mitochondria by complementation. Science. 1989;246(4929):500–3. pmid:2814477
View Article
PubMed/NCBI
Google Scholar

[156] View Article

[157] PubMed/NCBI

[158] Google Scholar

[ref41] 41. Schnaufer A, Clark-Walker GD, Steinberg AG, Stuart K. The F1-ATP synthase complex in bloodstream stage trypanosomes has an unusual and essential function. EMBO J. 2005;24(23):4029–40. pmid:16270030
View Article
PubMed/NCBI
Google Scholar

[160] View Article

[161] PubMed/NCBI

[162] Google Scholar

[ref42] 42. Lill R, Kispal G. Maturation of cellular Fe-S proteins: An essential function of mitochondria. Trends Biochem Sci. 2000;25(8):352–6. pmid:10916152
View Article
PubMed/NCBI
Google Scholar

[164] View Article

[165] PubMed/NCBI

[166] Google Scholar

[ref43] 43. Stehling O, Elsässer H-P, Brückel B, Mühlenhoff U, Lill R. Iron-sulfur protein maturation in human cells: Evidence for a function of frataxin. Hum Mol Genet. 2004;13(23):3007–15. pmid:15509595
View Article
PubMed/NCBI
Google Scholar

[168] View Article

[169] PubMed/NCBI

[170] Google Scholar

[ref44] 44. Schnaufer A, Domingo GJ, Stuart K. Natural and induced dyskinetoplastic trypanosomatids: How to live without mitochondrial DNA. Int J Parasitol. 2002;32(9):1071–84. pmid:12117490
View Article
PubMed/NCBI
Google Scholar

[172] View Article

[173] PubMed/NCBI

[174] Google Scholar

[ref45] 45. Sherman IW. Biochemistry of Plasmodium (malarial parasites). Microbiol Rev. 1979;43(4):453–95. pmid:94424
View Article
PubMed/NCBI
Google Scholar

[176] View Article

[177] PubMed/NCBI

[178] Google Scholar

[ref46] 46. Vaidya AB. Mitochondrial physiology as a target for atovaquone and other antimalarials. Sherman IW. Malaria: Parasite biology, pathogenesis, and protection. Washington, DC: ASM Press; 1998. 355–68.

Figures

Abstract

Introduction

Materials and methods

P. falciparum culture and transfection

Transfection vector generation

Growth assay

Immunofluorescence assays

Live cell microscopy

Western blotting

Immunopulldown and proteomics

Growth inhibition assay

Results

Generation of parasite lines conditionally expressing MPPα

MPPα is essential for parasite viability

Mitochondrial membrane potential is disrupted in knockdown parasites

Knockdown of MPPα expression causes hypersensitivity to proguanil

Proteomic analysis of MPPα immunoprecipitation

Discussion

Supporting information

S1 Fig. Maps of plasmids used for transfections.

S2 Fig. MPPα disruption in a second parasite line not expressing yDHOD.

S3 Fig. Susceptibility of MPPα - parasites to antimalarials remains unchanged.

S4 Fig. Proteomic analysis of MPPα immunoprecipitation.

S1 File. Primers and DNA sequences.

S2 File. Raw Images.

S1 Data. Dataset.

Acknowledgments

References