Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

  • Loading metrics

Open Access

Correction

Correction: Dynamic expression of cathepsin L in the black soldier fly (Hermetia illucens) gut during Escherichia coli challenge

  • Yun-Ru Chiang,
  • Han-Tso Lin,
  • Chao-Wei Chang,
  • Shih-Ming Lin,
  • John Han-You Lin

The images for Figs 4 to 7 are incorrectly switched. The image that appears as Fig 4 should be Fig 5, the image that appears as Fig 5 should be Fig 6, the image that appears as Fig 6 should be Fig 7 and the image that appears as Fig 7 should be Fig 4. The figure captions appear in the correct order. The authors have provided a corrected version of figures here.

thumbnail
Download:
Fig 4. Morphological organization and immunohistochemical localization of CTSL in BSFL.

The histology of BSFL is presented in longitudinal sections (A, B, C) and cross sections (D, E, F). H&E staining revealed the main structure of the fifth instar of BSFL (A, D). BSFL were stained with naïve mouse serum as a negative control (B, E). BSFL were stained by chromogenic IHC methods following injection of mice anti-BSFL CTSL serum (C, F). Positive signals were revealed in brown, while hematoxylin was used as a counterstain. H, head. (Bar = 500 μm).

https://doi.org/10.1371/journal.pone.0334338.g004

thumbnail
Download:
Fig 5. Relative expression levels of CTSL mRNA in the different larval stages.

Relative mRNA expression (2 -ΔΔct) levels of CTSL were detected using SRBR green assay and normalized to β-actin. The fold-change of CTSL expression was calculated for the 3rd, 4th, 5th, and 6th instars and compared with the 2nd instar. Bars represent mean ± S.E. (n = 3). Letters indicate significant differences (p < 0.05).

https://doi.org/10.1371/journal.pone.0334338.g005

thumbnail
Download:
Fig 6. Dynamic expression of CTSL mRNA in response to E. coli challenge.

Relative mRNA expression (2 -ΔΔct) levels of CTSL were detected using SRBR green assay and normalized to β-actin in the 2nd (A), 3rd (B), 4th (C), 5th (D), and 6th (E) instars. The fold-change of CTSL expression was calculated at 1.5, 3, 6, 9, and 12 h post-stimulation with E. coli and compared with 0 h (negative control was non-treated BSFL). Bars represent mean ± S.E. (n = 3). Statistical significance is indicated with lowercase letters (p < 0.05).

https://doi.org/10.1371/journal.pone.0334338.g006

thumbnail
Download:
Fig 7. Dynamic proteolytic activity of CTSL in response to E. coli challenge.

Percentage of CTSL activity in the fifth instar under E. coli challenge compared to negative control (cysteine proteinase inhibitor E-64). Error bars represent mean ± S.E. (n = 5). Statistical significance is indicated with different lowercase letters (p < 0.05).

https://doi.org/10.1371/journal.pone.0334338.g007

Reference

  1. 1. Chiang Y-R, Lin H-T, Chang C-W, Lin S-M, Lin JH-Y. Dynamic expression of cathepsin L in the black soldier fly (Hermetia illucens) gut during Escherichia coli challenge. PLoS One. 2024;19(3):e0298338. pmid:38451906

Citation: Chiang Y-R, Lin H-T, Chang C-W, Lin S-M, Lin JH-Y (2025) Correction: Dynamic expression of cathepsin L in the black soldier fly (Hermetia illucens) gut during Escherichia coli challenge. PLoS One 20(10): e0334338. https://doi.org/10.1371/journal.pone.0334338

Published: October 10, 2025

Copyright: © 2025 Chiang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.