https://orcid.org/0009-0003-6038-4173
Figures
Abstract
Background
Human papillomavirus (HPV) is the most prevalent sexually transmitted infection. Copper is essential for immune function, but its association with HPV infection remains unclear. This study aims to investigate the relationship between dietary copper intake and HPV infection.
Methods
This cross-sectional study analyzed 8,071 participants from the National Health and Nutrition Examination Survey (2003–2016). Copper intake was assessed using two 24-hour recalls, and HPV status was confirmed by DNA testing. Weighted multivariable logistic regression and restricted cubic splines (RCS) were used.
Results
After adjusting for multiple confounders, dietary copper intake was significantly inversely associated with vaginal HPV infection (odds ratio [OR], 0.79; 95% confidence interval [CI], 0.67–0.92). Compared with women in the lowest quartile of dietary copper intake, those in the highest quartile had a lower adjusted OR for vaginal HPV infection (OR, 0.60; 95% CI, 0.48–0.73). RCS analysis revealed an L-shaped association with a threshold at 1.2 mg/day of copper intake. Subgroup analyses showed that marital status moderated the association between copper intake and HPV infection (P for interaction < 0.001), with significance in those married or living with a partner but not in those living alone.
Citation: Feng C, Lu X, Fan Z, Wang X (2025) Negative association between dietary copper intake and human papillomavirus infection: A cross-sectional analysis of the National Health and Nutrition Examination Survey. PLoS One 20(10): e0333901. https://doi.org/10.1371/journal.pone.0333901
Editor: Ricardo Ney Oliveira Cobucci, UFRN: Universidade Federal do Rio Grande do Norte, BRAZIL
Received: June 18, 2025; Accepted: September 21, 2025; Published: October 13, 2025
Copyright: © 2025 Feng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: Data Availability Statement: All data used in this study are publicly available from the NHANES 2003–2016 cycles. The individual cycles can be accessed directly as follows (accessed on March 26, 2025).: 2003–2004: https://wwwn.cdc.gov/nchs/nhanes/continuousnhanes/default.aspx?BeginYear=2003 2005–2006: https://wwwn.cdc.gov/nchs/nhanes/continuousnhanes/default.aspx?BeginYear=2005 2007–2008: https://wwwn.cdc.gov/nchs/nhanes/continuousnhanes/default.aspx?BeginYear=2007 2009–2010: https://wwwn.cdc.gov/nchs/nhanes/continuousnhanes/default.aspx?BeginYear=2009 2011–2012: https://wwwn.cdc.gov/nchs/nhanes/continuousnhanes/default.aspx?BeginYear=2011 2013–2014: https://wwwn.cdc.gov/nchs/nhanes/continuousnhanes/default.aspx?BeginYear=2013 2015–2016: https://wwwn.cdc.gov/nchs/nhanes/continuousnhanes/default.aspx?BeginYear=2015.
Funding: The author(s) received no specific funding for this work.
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: HPV, human papillomavirus; NHANES, National Health and Nutrition Examination Survey; RCS, restricted cubic splines; DNA, deoxyribonucleic acid; BMI, body mass index; PIR, poverty-income ratio; OR, odds ratios; CI, confidence interval; USDA, United States Department of Agriculture; ATP7A, ATPase, Cu2+ transporting, alpha polypeptide; ATP7B, ATPase, Cu2+transporting, beta polypeptide
Introduction
Human papillomavirus (HPV) has emerged as one of the most significant global health challenges, representing the most prevalent sexually transmitted infection with profound clinical consequences. As the etiological agent responsible for over 99% of cervical cancer cases, HPV contributes to more than 300,000 annual deaths worldwide, with particularly heavy disease burdens observed in low- and middle-income countries where screening infrastructure remains limited [1–3]. Beyond its well-established role in cervical carcinogenesis, HPV infection demonstrates concerning associations with various anogenital and oropharyngeal malignancies, while also causing substantial reproductive morbidity and psychosocial distress among affected individuals [4–6].
The development of prophylactic vaccines has undoubtedly transformed HPV prevention paradigms, yet significant limitations persist that necessitate complementary approaches. While vaccination programs in developed nations have achieved notable success in reducing vaccine-type HPV incidence [7,8], three critical gaps remain: First, economic and logistical barriers continue to limit vaccination coverage in many regions [9,10]. Second, the vaccines demonstrate no therapeutic efficacy against established infections [11,12]. Most importantly, approximately one-third of oncogenic HPV types fall outside current vaccine protection, sustaining persistent infection and subsequent malignancy risks [12].
These limitations have stimulated growing interest in alternative prevention strategies, particularly through modifiable lifestyle factors such as nutrition. Among potential dietary components, copper presents a particularly compelling yet understudied candidate due to its dual physiological roles [13]. As an essential trace element, copper serves as a critical cofactor for numerous enzymes involved in fundamental biological processes including energy metabolism, antioxidant defense, and neurotransmitter synthesis [14]. Its unique redox properties enable participation in both beneficial immune responses and potentially harmful oxidative pathways.
Copper is an essential nutrient for optimal immune function and has been shown to possess antiviral properties [15,16]. Studies have demonstrated that copper deficiency in rodents leads to decreased CD4 T-cell counts [16] and impairs specific CD4 T-cell responses to the HPV E6 protein, which may be critical for HPV clearance [17]. Moreover, copper metabolism within cells may directly interact with the HPV life cycle. For instance, copper ions can modulate the intracellular redox state, which may significantly affect HPV replication and transcription [18]. Excess or deficiency of copper ions may influence HPV infection efficiency and viral load by altering intracellular oxidative stress levels.
Notably, research specifically examining dietary copper intake in relation to HPV infection remains surprisingly limited, despite copper’s obligatory dependence on external sources since it cannot be endogenously synthesized or degraded [19]. Previous investigations have predominantly focused on serum copper levels or tissue concentrations, which may not accurately reflect long-term nutritional status. Furthermore, existing studies have generally failed to account for potential effect modification by behavioral factors such as sexual activity patterns, despite well-documented associations between sexual behavior and HPV acquisition risk.
To address these critical knowledge gaps, the primary aim of this study is to use data from the National Health and Nutrition Examination Survey (NHANES) to examine the relationship between dietary copper intake and HPV infection. Specifically, we aim to determine whether there is an optimal range of dietary copper intake for HPV prevention, while considering important potential confounders and effect modifiers.
Methods
Ethics statement
This study involved human participants and/or tissue and was conducted according to the guidelines laid down in the Declaration of Helsinki. The NHANES survey protocol was approved by the National Center for Health Statistics (NCHS) Research Ethics Review Board (ERB). All participants provided written informed consent.
Type of study
This is a cross-sectional analysis of seven consecutive 2-year cycles of the NHANES conducted from 2003 through 2016 to examine the association between dietary copper intake and prevalent HPV infection among U.S. women.
Study area
The data originate from the ongoing NHANES, a nationally representative program that employs a complex, stratified, multistage probability cluster sampling design in which primary sampling units are geographic counties (n = 30 per cycle) selected across the entire United States, followed by random selection of households and individuals within strata with deliberate oversampling of minority groups, the elderly, and low-income populations to enhance statistical power; all data collection activities—household interviews, Mobile Examination Center visits, and laboratory analyses—were integrated across the United States under the standardized NHANES protocol.
Study design
A survey-weighted, complex sampling framework—incorporating stratification (SDMVSTRA), clustering (SDMVPSU), and the dietary two-day integrated weight (WTDR2D) to account for differential selection probabilities, non-response bias in dietary recalls, and post-stratification to U.S. Census benchmarks—was applied to yield nationally representative estimates.
Study sample
Female participants aged 18–59 years across the 2003–2016 NHANES cycles who had information on genital HPV infection constituted the source population (n = 14,841); participants were sequentially excluded if they lacked data on genital HPV infection status (n = 2,096), dietary copper intake (n = 1,854), or any of the required covariates—marital status (n = 545), family income (n = 632), education level (n = 436), body mass index (n = 36), diabetes (n = 146), hypertension (n = 19), number of sexual partners in the past year (n = 841), smoking status (n = 1), age of first sex (n = 154), and alcohol drinking (n = 10)—yielding a final analytic sample of 8,071 women with a mean age of 40.0 (SE 0.3) years and a mean age at first sexual intercourse was 18.2 (SE 0.4) years, participant flow is depicted in Fig 1.
NHANES, National Health and Nutrition Examination Survey; HPV, human papillomavirus.
Principles of the study
The study adhered to the NHANES analytical guidelines and adopted a complete-case approach for the primary analysis. The anonymity of the publicly released data was maintained throughout.
Data provenance and sources
Anonymized data were downloaded directly from the NHANES website, and survey weights were applied to account for nonresponse and ensure national representativeness; no additional primary data collection was performed.
Exposure assessment – dietary copper intake
Data on dietary copper intake were derived from two nonconsecutive 24-hour dietary recalls in the NHANES. The first recall was conducted in a mobile examination center, and the second was administered via telephone 3 to 10 days later. Both recalls were facilitated by a computer-assisted dietary interview system. Copper concentrations in foods (unit: mg/100g) were obtained from the corresponding versions of the United States Department of Agriculture (USDA)‘s Food and Nutrition Database for Dietary Studies (version 1.0 for 2003–2004, up to version 7.0 for 2015–2016), which directly integrated nutrient data from the USDA Standard Reference legacy database. The total daily copper intake was determined by averaging the copper intake values from the two separate 24-hour dietary recall surveys, excluding dietary supplements. Composite foods were broken down using USDA recipe files. Dietary copper intake was stratified into quartiles: Q1 ranged from 0 to 0.76 mg/day, Q2 from 0.77 to 1.02 mg/day, Q3 from 1.03 to 1.34 mg/day, and Q4 from 1.35 mg/day and above.
Outcome
In this study, we utilized the Roche Linear Array HPV Genotyping Test to detect 37 types of HPV from self-collected vaginal swab samples. Participants collected samples using standard vaginal swabs, which were then placed in SurePath preservative fluid and transported on dry ice to the CDC laboratory at −70°C. DNA was extracted using the QIAamp DNA Mini Kit (concentration ≥50 ng/μL). HPV L1 gene and β-globin internal control were amplified by PCR using PGMY09/11 primers. The amplified products were subjected to reverse line blot hybridization using the BeeBlot automated system to detect 37 types of HPV (6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 45, 51, 52, 53, 54, 55, 56, 58, 59, 61, 62, 64, 66, 67, 68, 69, 70, 71, 72, 73, 81, 82, 83, 84, 89, IS39). Quality control measures included β-globin positivity verification (>5 copies, samples not meeting the criteria were excluded), retesting of HPV52-positive samples using the LightCycler 480 quantitative PCR (threshold of ≥5 copies/10 μL to exclude cross-reactivity with HPV33/35/58). According to NHANES protocol, each strip was read independently by two technologists blinded to each other; discrepancies were resolved by a senior reviewer, with inclusion of negative and positive controls in each batch (containing 50 copies of HPV16 plasmid). A positive result for any HPV type was defined as a positive HPV infection (binary variable). For detailed methodology, refer to the Roche manual and the NHANES laboratory handbook.
Covariates
Based on previous scientific literature and clinical insights, we evaluated a range of potential covariates, including age, race, education level, marital status, family income, body mass index (BMI), diabetes, hypertension, alcohol drinking, smoking status, age of first sex, and number of sexual partners in the past year. Race was categorized into non-Hispanic White, non-Hispanic Black, Mexican American, or other races. Education level was divided into three groups based on years of education: less than 9 years, 9–12 years, or more than 12 years. Marital status was classified as living alone (never married, separated, divorced, or widowed) or living with a partner (married or cohabiting). Family income was stratified into three levels according to the poverty-income ratio (PIR): low-income (PIR ≤ 1.3), middle-income (PIR = 1.3–3.5), and high-income (PIR > 3.5). BMI (kg/m²) was calculated using standardized anthropometric measurements collected during the health examination, defined as weight (kg) divided by height (m) squared. The presence of preexisting conditions, such as hypertension and diabetes, was determined based on self-reported diagnoses by physicians or health professionals. Alcohol drinking status was assessed by whether participants consumed at least 12 drinks of any type of alcoholic beverage in any given year. Smoking status was classified into three categories: never smokers (fewer than 100 cigarettes), current smokers, and former smokers (those who quit smoking after consuming more than 100 cigarettes). Sexual history was ascertained from responses to the sexual behavior questionnaire, with the number of sexual partners in the past year categorized as 0, 1, or 2 or more. Additionally, we assessed collinearity among the covariates by calculating the variance inflation factor and examining the correlation matrix. All VIF values were below 10, and none of the correlation coefficients reached statistical significance. This indicates that there is no substantial collinearity among the selected covariates, ensuring the stability of the model estimates and the reliability of the results.
Statistical analyses
Consistent with NHANES analytical guidelines, we used the previously described complex survey features—stratification (SDMVSTRA), clustering (SDMVPSU), and the WTDR2D weight—to obtain nationally representative estimates. Our primary analysis employed a complete-case approach after excluding participants with missing data on any covariate. Categorical variables are presented as unweighted counts (weighted percentages), and continuous variables are presented as means ± standard error. Group differences for continuous variables were assessed using one-way ANOVA, and associations for categorical variables were assessed using chi-square tests. Weighted multivariable logistic regression was used to calculate the odds ratios (OR) and 95% confidence intervals (CI) for the association between dietary copper intake and vaginal HPV infection. We defined four models: Model 1 was unadjusted; Model 2 adjusted for age, race, education level, marital status, and family income; Model 3 added BMI, diabetes, hypertension, alcohol use, and smoking status to Model 2; and Model 4 further incorporated age of first sex and number of sexual partners in the past year into Model 3. To explore potential effect modification, we generated forest plots for interactions across different subgroups. A Bonferroni correction was applied to account for multiple comparisons in the five prespecified subgroup analyses (age, marital status, family income, alcohol drinking, and number of sexual partners). Linearity assumptions for continuous variables were assessed using restricted cubic splines (RCS) with knots at the 10th, 50th, and 90th percentiles of dietary copper intake, optimized via Akaike Information Criterion minimization. Influential observations were evaluated through residual-leverage contour plots incorporating predefined Cook’s distance thresholds (critical d = 0.5) (S1 Fig). Given documented limitations of the Hosmer–Lemeshow test in large samples [20], model fit was assessed using LOESS calibration curves comparing predicted probabilities against observed event rates (S2 Fig).
Robustness was evaluated by three sensitivity analyses: exclusion of participants with cancer to remove immune compromise, exclusion of vaccinated participants to eliminate vaccine-induced bias, and multiple imputation (5 replications, chained equations via R mice procedure) to address missing-data bias; no substantive changes in conclusions were observed.
All statistical analyses were conducted using R Statistical Software (Version 4.2.2, http://www.R-project.org, The R Foundation) and Free Statistics Analysis Platform (Version 2.1.1, Beijing, China, http://www.clinicalscientists.cn/free statistics).
Results
We employed several analytical strategies to examine the relationship between copper intake and HPV infection. First, we used multivariable regression models to assess the overall association. Second, we used nonlinear models to identify potential threshold effects. Finally, we conducted sensitivity analyses to test the robustness of our findings.
Characteristics of the participants
The baseline characteristics of excluded participants (those with missing covariate data) and included participants (those with complete covariate data) are presented in S1 Table. Overall, the baseline data appear stable; however, we cannot rule out the potential impact of non-random missing data on the results. Table 1 summarizes the baseline characteristics of the 8,071 participants, stratified by quartiles of dietary copper intake. Participants in higher intake quartiles were generally older, more likely to be Non-Hispanic White, married or living with a partner, and had higher educational attainment and family income. Health outcomes and behaviors also varied significantly across quartiles. Notably, mean BMI and the proportion of current smokers were highest in the lowest quartile (Q1: 29.3 kg/m² and 34.8%, respectively) and lowest in the highest quartile (Q4: 28.1 kg/m² and 15.1%, respectively) (P < 0.001). The prevalence of hypertension was also lowest in Q4 (14.1%, P = 0.008). No significant difference was observed for diabetes across the groups (P = 0.140).
Association between dietary copper intake and HPV infection
Univariate analysis showed that marital status, family income, alcohol consumption, age at first sex, and the number of sexual partners in the past year were associated with HPV infection (S2 Table).
Table 2 presents the results of multivariate regression analyses examining the association between dietary copper intake and vaginal HPV infection. When dietary copper was modeled as a continuous variable, each 1 mg/day increase in intake was associated with a significantly reduced odds of HPV infection after full adjustment for demographic, socioeconomic, health behavior, and sexual activity covariates (OR = 0.79; 95% CI: 0.67–0.92; P = 0.003). When analyzed by quartiles, a significant inverse dose-response trend was observed across increasing quartiles of copper intake (P for trend <0.001). Compared to the lowest quartile (Q1, ≤ 0.76 mg/day), the fully adjusted ORs for Q2, Q3, and Q4 were 0.83 (95% CI: 0.67–1.03; P = 0.087), 0.65 (95% CI: 0.53–0.81; P < 0.001), and 0.60 (95% CI: 0.49–0.75; P < 0.001), respectively. The strength of the association was attenuated but remained significant with sequential adjustment for confounders, most notably in Q4.
RCS analysis demonstrated an L-shaped relationship between dietary copper intake and HPV infection (nonlinear, P < 0.001; Fig 2). Table 3 presents the results of the threshold analysis using a two-piecewise regression model, this analysis identified a nonlinear relationship between dietary copper intake and the likelihood of HPV infection. Participants with dietary copper intake ≤1.2 mg/day had 55% lower odds of HPV infection (adjusted OR 0.45; 95% CI 0.29–0.70; P < 0.001). However, when daily copper intake was equal to or greater than 1.2 mg/day, further increases in copper intake did not show a significant correlation with HPV infection prevalence (P = 0.743). This pattern suggests a saturable protective effect, where achieving a minimum intake level is critical, but exceeding it does not further reduce risk.
HPV, human papillomavirus. NHANES, National Health and Nutrition Examination. The blue solid line indicates the smooth curve fit between the variables, the shaded area surrounding the line represents the 95% confidence interval. The restricted cubic spline model was adjusted for age, race, education level, marital status, family income, body mass index, diabetes, hypertension, alcohol drinking, smoke status, age of first sex, number of sexual partners in the past year. The plot displays only 99% of the data.
Subgroup analysis and sensitivity analyses
A stratified analysis assessed potential effect modification of dietary copper intake on HPV infection across multiple subgroups. After stratifying by age, marital status, family income, alcohol drinking, and number of sexual partners in the past year, after Bonferroni correction for five subgroup comparisons (α = 0.01), only marital status demonstrated a statistically significant interaction effect (adjusted P = 0.005). This modification effect was observed in individuals who were married or living with a partner (OR: 0.68, 95% CI: 0.53–0.86) but not in those living alone (OR: 0.95, 95% CI: 0.80–1.13). No significant interactions were observed in other subgroups after multiplicity adjustment (Fig 3).
HPV, human papillomavirus. OR, odds ratio. CI, confidence interval. * P value for interaction is significant after Bonferroni correction. Except for the stratification factor itself, the stratifications were adjusted for all variables (age, race, education level, marital status, family income, body mass index, diabetes, hypertension, alcohol drinking, smoke status, age of first sex, number of sexual partners in the past year).
Table 4 summarizes the results of sensitivity analyses performed to assess the robustness of the association between dietary copper intake and HPV infection. After full adjustment, the association remained significant across all scenarios: after excluding participants with cancer (adjusted OR = 0.79; 95% CI: 0.68–0.93), after excluding HPV-vaccinated individuals (adjusted OR = 0.75; 95% CI: 0.60–0.93), and after multiple imputation for missing data (adjusted OR = 0.85; 95% CI: 0.76–0.98). These results demonstrate the consistency of the protective association under different analytical assumptions.
Discussion
The association between dietary micronutrients and HPV infection, a primary cause of cervical cancer, remains an area of active investigation. This study aimed to explore whether dietary copper intake is associated with the prevalence of vaginal HPV infection among U.S. women. In this nationally representative large cross-sectional study, after adjusting for potential confounders, an inverse correlation was found between dietary copper intake and vaginal HPV infection in American women, with a threshold saturation effect at 1.2 mg/day. This association remained significant after excluding cancer patients and those vaccinated against HPV, as well as after applying multiple imputation for missing covariate data. Subgroup analysis revealed a stronger link among individuals who are married or living with a partner.
While the immunomodulatory functions of copper are widely recognized, epidemiological research on the association between dietary copper and HPV infection remains limited. Two key studies provide preliminary evidence: Barchitta et al., based on a cross-sectional study in Italy, found that Mediterranean dietary patterns rich in copper (such as nuts and legumes) were significantly associated with a reduced risk of persistent high-risk HPV infection [21]. Xiao et al. demonstrated that adequate dietary copper intake was associated with a lower prevalence of high-risk HPV infection, although the dose-response relationship was not explored [22]. Building on this foundation, the present study quantified this non-linear relationship using RCS modeling. Furthermore, subgroup analyses revealed that the protective effect of copper was more pronounced among women who were married or living with a partner. These findings provide novel epidemiological evidence for developing targeted dietary copper intervention strategies to prevent HPV infection.
Copper plays a complex role in viral infections, and its association mechanisms with HPV have not yet been fully elucidated, potentially involving multiple metabolic pathways. Existing research has demonstrated that viral infections can disrupt host copper metabolism, induce the production of reactive oxygen species, and subsequently trigger oxidative stress and autophagy [23,24]. Moreover, viral infections can also interfere with the host cell’s endocytic pathways, such as the sorting mechanisms mediated by SNX27, leading to abnormal localization and metabolic disturbances of copper transporters ATP7A (ATPase, Cu2+ transporting, alpha polypeptide) and ATP7B (ATPase, Cu++ transporting, beta polypeptide) [25]. Additionally, copper ions may exert direct antimicrobial effects by disrupting the protein structures of pathogens [26]. Copper transporters ATP7A/B, which are transported by the molecular chaperone protein Atox1, are core molecules in regulating copper homeostasis [27]. Maintaining copper homeostasis is crucial for supporting antiviral immunity, and systemic copper imbalances may impact immune function. Given the importance of copper in immunity, maintaining copper homeostasis may help reduce the risk of persistent HPV infection. We posit that the interplay between viral infections and copper metabolism will provide novel insights into HPV-related clinical syndromes and pave the way for antiviral strategies targeting the copper pathway. Furthermore, adequate copper intake is associated with reduced HPV prevalence, though causality requires verification via intervention studies.
The study has several strengths. Utilizing the weighted NHANES database ensures that the results are representative of women in the United States, providing objective evidence for the prevention and treatment of HPV infections. The research highlights that individuals who are married or living with a partner may have a greater need for copper intake, offering a basis for targeted interventions in high-risk HPV populations. Additionally, the study identifies a threshold for copper intake, which can accurately guide daily copper consumption. Overall, these findings lay a solid foundation for future research on the relationship between dietary copper and HPV infection.
This study has several limitations. First, the cross-sectional NHANES design inherently precludes causal inference between copper intake and HPV infection. Critically, we cannot establish temporal precedence—specifically, whether low copper levels causally preceded HPV exposure or were a consequence of metabolic alterations related to infection. Reverse causation remains plausible, as HPV-induced physiological changes may alter dietary behaviors and nutrient absorption. Second, because of the single-time HPV status measurement, we cannot assess the impact of copper intake on HPV acquisition or persistence; large-scale prospective studies are needed. Third, despite comprehensive adjustment for covariates, residual confounding may persist from unmeasured factors, including partners’ HPV status and precise zinc intake, given the biological interplay between zinc and copper. Fourth, while NHANES sampling weights ensure nationally representative estimates for non-institutionalized U.S. adult women, these findings are not generalizable to other populations—including men, non-U.S. cohorts, or clinical subgroups—without external validation. Fifth, inter-rater reliability (κ) for HPV genotyping could not be determined because the dual-reader worksheets were not retained. To address these constraints, future research should: (1) implement prospective cohorts establishing temporal relationships while controlling reverse causation; (2) conduct RCTs testing copper supplementation in identified high-risk subgroups (e.g., married/cohabiting women); and (3) conduct external validation in men, non-U.S. populations, and diverse clinical subgroups to establish the generalizability of the copper–HPV association.
Conclusions
The findings of this cross-sectional study indicate that dietary copper intake is negatively correlated with HPV infection in American women. Individuals who are married or living with a partner may have a greater need for copper intake. These results may provide a novel basis and direction for future research investigating factors associated with reduced risk of HPV infections.
Supporting information
S1 Fig. Residual-leverage plot with Cook’s distance contours for influential point assessment.
This plot illustrates the relationship between leverage values and standardized residuals. The green dashed lines represent Cook’s distance contours, with all data points falling within these contours – thus ruling out influential observations.
https://doi.org/10.1371/journal.pone.0333901.s001
(TIF)
S2 Fig. LOESS calibration curve for the final multivariable model.
The smoothed LOESS line closely tracks the ideal calibration line, indicating excellent agreement between predicted probabilities and observed event rates. Calibration slope = 1.00 (95% CI 0.94–1.06), intercept = 0.00 (95% CI –0.05–0.05).
https://doi.org/10.1371/journal.pone.0333901.s002
(TIF)
S1 Table. The basic characteristics of the excluded (n = 2820) and included (n = 8071) participants.
BMI, body mass index. Q1–Q4, quartiles based on dietary copper consumption. HPV, human papillomavirus.
https://doi.org/10.1371/journal.pone.0333901.s003
(DOCX)
S2 Table. Association of covariates and HPV status in women.
HPV, human papillomavirus. OR, Odds Ratio. CI, Confidence Interval.
https://doi.org/10.1371/journal.pone.0333901.s004
(DOCX)
Acknowledgments
We gratefully thank Jie Liu of the Department of Vascular and Endovascular Surgery, Chinese PLA General Hospital for his contribution to the statistical support, study design consultations, and comments regarding the manuscript.
References
- 1. Molina MA, Steenbergen RDM, Pumpe A, Kenyon AN, Melchers WJG. HPV integration and cervical cancer: a failed evolutionary viral trait. Trends Mol Med. 2024;30(9):890–902. pmid:38853085
- 2. Jin S, Lin F, Yang L, Zhang Q. Association between dietary folate intake and HPV infection: NHANES 2005-2016. PLoS One. 2024;19(7):e0306636. pmid:38995887
- 3. Correction to Lancet Glob Health 2020; 8: e191-203. Lancet Glob Health. 2022;10(1):e41. pmid:34919855
- 4. Hu H, Wu Y, Zhao M, Liu J, Xie P. Sleep duration time and human papillomavirus infection risk: the U-shaped relationship revealed by NHANES data. PLoS One. 2024;19(4):e0301212. pmid:38578744
- 5. Sucato A, Buttà M, Bosco L, Di Gregorio L, Perino A, Capra G. Human papillomavirus and male infertility: what do we know? Int J Mol Sci. 2023;24(24):17562. pmid:38139389
- 6. Jensen JE, Becker GL, Jackson JB, Rysavy MB. Human papillomavirus and associated cancers: a review. Viruses. 2024;16(5):680. pmid:38793561
- 7. Spayne J, Hesketh T. Estimate of global human papillomavirus vaccination coverage: analysis of country-level indicators. BMJ Open. 2021;11:e052016.
- 8. Kim J, Choe YJ, Park J, Cho J, Cheong C, Oh J-K, et al. Comparative effects of bivalent, quadrivalent, and nonavalent human papillomavirus vaccines in the prevention of genotype-specific infection: a systematic review and network meta-analysis. Infect Chemother. 2024;56(1):37–46. pmid:38014729
- 9. Ewongwo A, Sahor AF, Ngwa W, Nwachukwu C. A guide to global access to HPV vaccination to all women in low- and middle-income countries; a minireview of innovation and equity. Front Oncol. 2024;14:1380663. pmid:39416459
- 10. Guillaume D, Waheed D-E-N, Schleiff M, Muralidharan KK, Vorsters A, Limaye RJ. Global perspectives of determinants influencing HPV vaccine introduction and scale-up in low- and middle-income countries. PLoS One. 2024;19(1):e0291990. pmid:38227567
- 11. Cao Q, Hou Y, Wang C, Yin J. Effect of human papillomavirus (HPV) vaccination on HPV infection and recurrence of HPV related disease after local surgical treatment: a systematic review and meta-analysis. PLoS One. 2024;19(12):e0312128. pmid:39739895
- 12. Di Donato V, Caruso G, Bogani G, Cavallari EN, Palaia G, Perniola G, et al. HPV vaccination after primary treatment of HPV-related disease across different organ sites: a multidisciplinary comprehensive review and meta-analysis. Vaccines (Basel). 2022;10(2):239. pmid:35214697
- 13. Ren X, Luo X, Wang F, Wan L, Wang X, Xiong J, et al. Recent advances in copper homeostasis-involved tumor theranostics. Asian J Pharm Sci. 2024;19(5):100948. pmid:39474127
- 14. Wang Z, Zhao Y, Zhao Y, Zhang Y, Yao X, Hang R. Exosomes secreted by macrophages upon copper ion stimulation can promote angiogenesis. Mater Sci Eng C Mater Biol Appl. 2021;123:111981. pmid:33812609
- 15. Sagripanti JL, Lightfoote MM. Cupric and ferric ions inactivate HIV. AIDS Res Hum Retroviruses. 1996;12(4):333–7. pmid:8906994
- 16. O’Dell BL. Interleukin-2 production is altered by copper deficiency. Nutr Rev. 1993;51(10):307–9. pmid:8302489
- 17. Kim KH, Greenfield WW, Cannon MJ, Coleman HN, Spencer HJ, Nakagawa M. CD4+ T-cell response against human papillomavirus type 16 E6 protein is associated with a favorable clinical trend. Cancer Immunol Immunother. 2012;61(1):63–70. pmid:21842207
- 18. Chaturvedi UC, Shrivastava R. Interaction of viral proteins with metal ions: role in maintaining the structure and functions of viruses. FEMS Immunol Med Microbiol. 2005;43(2):105–14. pmid:15681139
- 19. Conforti RA, Delsouc MB, Zorychta E, Telleria CM, Casais M. Copper in gynecological diseases. Int J Mol Sci. 2023;24(24):17578. pmid:38139406
- 20. Kramer AA, Zimmerman JE. Assessing the calibration of mortality benchmarks in critical care: the Hosmer-Lemeshow test revisited. Crit Care Med. 2007;35(9):2052–6. pmid:17568333
- 21. Barchitta M, Maugeri A, Quattrocchi A, Agrifoglio O, Scalisi A, Agodi A. The association of dietary patterns with high-risk human papillomavirus infection and cervical cancer: a cross-sectional study in Italy. Nutrients. 2018;10(4):469. pmid:29641467
- 22. Xiao D, Li W, Zhang W-H, Wen Z, Dai B, Mo W, et al. Dietary zinc, copper, and selenium intake and high-risk human papillomavirus infection among American women: data from NHANES 2011-2016. Nutr Cancer. 2022;74(6):1958–67. pmid:34553663
- 23. Jung KI, Pyo CW, Choi S-Y. Influenza A virus-induced autophagy contributes to enhancement of virus infectivity by SOD1 downregulation in alveolar epithelial cells. Biochem Biophys Res Commun. 2018;498(4):960–6. pmid:29548827
- 24. Puig-Pijuan T, Souza LRQ, Pedrosa C da SG, Higa LM, Monteiro FL, Tanuri A, et al. Copper regulation disturbance linked to oxidative stress and cell death during Zika virus infection in human astrocytes. J Cell Biochem. 2022;123(12):1997–2008. pmid:36063501
- 25. Ren Y, Lv L, Li P, Zhang L. Inhibition of endocytic recycling of ACE2 by SARS-CoV-2 S protein partially explains multiple COVID-19 related diseases caused by ACE2 reduction. J Infect. 2022;85(1):e21–3. pmid:35421520
- 26. Dupont CL, Grass G, Rensing C. Copper toxicity and the origin of bacterial resistance--new insights and applications. Metallomics. 2011;3(11):1109–18. pmid:21984219
- 27. Festa RA, Thiele DJ. Copper: an essential metal in biology. Curr Biol. 2011;21(21):R877-83. pmid:22075424