Figures
In the published version of this article [1], the figure legends for Figs 3–7 were published in the incorrect order. The authors have provided a corrected version of figures and captions here.
(a) PCA plot, and (b) hierarchical clustering and heatmap of the Log2 fold change in expression relative to unstimulated samples of the innate immune genes in response to LPS, Pam3CSK4 and R848 24-hour whole blood stimulation in control (n = 5) and BRD diagnosed (Case) (n = 6) calves aged 2-8 weeks old. (c–d) Boxplots of the fold change in expression relative to unstimulated samples of significant genes found between control (n = 5) and BRD diagnosed (Case) (n = 6) in response to (c) Pam3CSK4 and (d) LPS after p value adjustment. p adjusted<0.1*. The box represents the interquartile range, the whiskers the upper and lower limits and the dots representing each calf. T-tests were used to calculate significant differences with p-values adjusted by FDR method. *p-adjusted <0.1.
(a) IL-1 and inflammasome, (c) chemokine and (e) IL-6 pathway gene expression z-scores in response to Pam3CSK4 in whole blood of controls and BRD diagnosed calves aged 2-8 weeks old. Error bars represent mean ± standard deviation. T-tests were used to test for significant differences between gene expression scores. *p < 0.05, **p < 0.01. (b) IL-1 and inflammasome, (d) chemokine and (f) IL-6 pathway hierarchical clustering and heatmap of centred and scaled per unit variance LogFC gene expression relative to unstimulated samples in BRD blood of controls and BRD diagnosed calves aged 2–8 weeks old.
Boxplots of IL-6, IL-1β and IL-8 protein expression in 24-hour unstimulated whole blood of calves that were controls and (a) TUS positive and (b) WHS positive. The box represents the interquartile range, the whiskers the upper and lower limits and the dots representing each calf with any calves outside the upper and lower limits considered outliers (Tukey outlier analysis). T tests were used to test for significant differences between cases and controls. *p < 0.05.
Boxplots of (a and b) IL-6, (c and d) IL-1β and (e and f) IL-8 protein expression in 24-hour stimulated whole blood with PAMPs LPS, Pam3CSK4 and R848 in 2-8 weeks old control, (a, c and e) TUS positive, and (b, d and f) WHS positive calves. The box represents the interquartile range, the whiskers the upper and lower limits and the dots representing each calf with any calves outside the upper and lower limits considered outliers (Tukey outlier analysis). T tests were used to test for significant differences between cases and controls. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
ROC curves (x = false positives, y = true positives) of IL-6 protein concentrations in response to LPS, Pam3CSK4 and R848 in control and TUS positive calves. AUC shows an 0.68, 0.83 and 0.66 probability and Youden index shows an optimum accuracy of 47.0%, 66.4% and 30.7% for IL-6 protein concentrations in response to LPS, Pam3CSK4 and R848 respectively in diagnosing BRD calves with pathological lung lesions determined by thoracic lung ultrasonography scoring.
Reference
Citation: Reid C, Donlon J, Remot A, Kennedy E, De Matteis G, O’Farrelly C, et al. (2025) Correction: Hyper-induction of IL-6 after TLR1/2 stimulation in calves with bovine respiratory disease. PLoS One 20(6): e0326136. https://doi.org/10.1371/journal.pone.0326136
Published: June 9, 2025
Copyright: © 2025 Reid et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.