Figures
After publication of this article [1], the authors discovered that the position of the 20 bp duplication in exon 2 of the bovine ALX4 gene was not correctly depicted in Fig 4. With the updated annotation of the ALX4 gene, the duplication is located 3 base pairs upstream at position 309 to 328 of the exon. The 3 bp shift also influences the depiction of the cDNA in Fig 5 and the amino acids at the exon boundaries in Figs 6, 7, and 8. The update of the position of the 20 bp duplication has no effect on the results and conclusions.
Here, the authors have provided updated Figs 4, 5, 6, 7, and 8 with the correct position of the 20 bp duplication in exon 2 of the bovine ALX4 gene.
The genomic structure of the bovine ALX4 gene as deduced from AC_00172 (Bos_taurus_UMD_3.1), NC_007313 (Btau_4.6.1), and NM_001030304 is depicted [33]. Sizes of intron 1 and 2 are not yet known due to larger gaps. Numbering of positions refers to AC_000172. Duplicated sequences in exon 2 and exon 4 in the affected animals are shown with gray bars. Numbers below the sequences indicate the corresponding nucleotide positions of the wildtype (wtex2, wtex4) and mutated (mutex2dup, mutex4dup) alleles within the corresponding exon. Positions of the SNPs and duplications according to HGVS [35] are listed in Table 2.
The coding sequences of the four ALX4 variants beginning in exon 2 are shown. The exonic regions are indicated with boxes. Stop codons are shown in bold and are underlined. Numbering refers to the respective nucleotide position within the variant.
Amino acid sequences were deduced from the coding sequences of the ALX4 variants. Dashes indicate identical amino acid sequences. Corresponding exonic regions are indicted with different colours. Amino acids encoded by exon 1 are shown in green, exon 2 in magenta, exon 3 in cyan, and exon 4 in grey. The altered amino acid sequence due to the exon 2 duplication is shown in yellow. The truncated amino acid sequence of the ALX4(mutex4dup)-variant is shown in plain text. Wt: Wild type.
Alignment of the predicted mutex2dup, mutex4dup, and mutex2dup/ex4dup ALX4 proteins. The location of the homeodomain consensus regions are indicated with open boxes. The highlighted amino acid positions are highly conserved functional residues in helix III. B: base contact site; G: paralog-group residue; H: hydrophobic core site; P: phosphate backbone contact site 59].
Protein structures were predicted using the SWISS-Model workspace 52, 53]. The human ALX4 homeodomain structure has been determined by solution NMR and was used for homology modelling 54]. The three homeodomain helices (helix I, helix II, helix III) and amino acids with corresponding locations are indicated. NT: N-terminal arm.
Reference
- 1. Brenig B, Schütz E, Hardt M, Scheuermann P, Freick M (2015) A 20 bp Duplication in Exon 2 of the Aristaless-Like Homeobox 4 Gene (ALX4) Is the Candidate Causative Mutation for Tibial Hemimelia Syndrome in Galloway Cattle. PLoS ONE 10(6): e0129208. https://doi.org/10.1371/journal.pone.0129208
Citation: Brenig B, Schütz E, Hardt M, Scheuermann P, Freick M (2024) Correction: A 20 bp Duplication in Exon 2 of the Aristaless-Like Homeobox 4 Gene (ALX4) Is the Candidate Causative Mutation for Tibial Hemimelia Syndrome in Galloway Cattle. PLoS ONE 19(9): e0310790. https://doi.org/10.1371/journal.pone.0310790
Published: September 19, 2024
Copyright: © 2024 Brenig et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.