C57B/6 mice were given 10 μg polyI:C o.p. on days 0–2. On either days 0–2 (group A), days 1–2 (group B) or day 2 (group C), mice were also given vehicle or 700 μg/kg budesonide o.p.. On D3, 0.2 mg 4kDa FITC-dextran was instilled o.p. and 1hr later BALF and blood were harvested. A-B) Total protein and albumin levels in BALF were assessed via Bradford and ELISA respectively. C-D) FITC-dextran levels in BALF and serum were analyzed using a fluorescent plate reader. E-F) Neutrophils were assessed via cytospin and H&E staining, and CXCL1 was quantified via ELISA. Data are mean ± standard deviation. One way ANOVA followed by unpaired Tukey’s multiple comparisons test. * p <0.05, **p<0.01, *p<0.001.

https://doi.org/10.1371/journal.pone.0306666.g001