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Open Access

Correction

Correction: AMPK-Activated Protein Kinase Suppresses Ccr2 Expression by Inhibiting the NF-κB Pathway in RAW264.7 Macrophages

  • Fumiaki Kumase,
  • Kimio Takeuchi,
  • Yuki Morizane,
  • Jun Suzuki,
  • Hidetaka Matsumoto,
  • Keiko Kataoka,
  • Ahmad Al-Moujahed,
  • Daniel E. Maidana,
  • Joan W. Miller,
  • Demetrios G. Vavvas

In Fig 2, the beta actin is incorrect. Please see the correct Fig 2 here.

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Fig 2. Pharmacological activation of AMPK counter-regulates Ccr2 expression in the LPS-stimulated M1 macrophages.

A: RAW264.7 macrophages treated with either control or AMPKα1 siRNA were additionally treated with 25–100 μM of the AMPK activator, A769662. The phosphorylation of AMPKα (p-AMPKα) after A769662 treatment was examined by Western blotting. β-actin was probed as an internal control. B: RAW264.7 macrophages were pretreated with 25–100 μM A769662 for 2 h, followed by co-treatment with 100 ng/ml of LPS and each different concentration of A769662 for 12 h. Dimethyl sulfoxide (DMSO) was used as a control. Ccr2 expression was analyzed by flow cytometry. C: RAW264.7 macrophages treated with either control or AMPKα1 siRNA were pretreated with 50 μM A769662 for 2 h, followed by co-treatment with 100 ng/ml of LPS and 50 μM A769662 for 12 h. DMSO was used as a control. Ccr2 expression was analyzed by flow cytometry. n = 3. *, p < 0.05; ***, p < 0.001.

https://doi.org/10.1371/journal.pone.0304894.g001

Reference

  1. 1. Kumase F, Takeuchi K, Morizane Y, Suzuki J, Matsumoto H, Kataoka K, et al. (2016) AMPK-Activated Protein Kinase Suppresses Ccr2 Expression by Inhibiting the NF-κB Pathway in RAW264.7 Macrophages. PLoS ONE 11(1): e0147279. pmid:26799633

Citation: Kumase F, Takeuchi K, Morizane Y, Suzuki J, Matsumoto H, Kataoka K, et al. (2024) Correction: AMPK-Activated Protein Kinase Suppresses Ccr2 Expression by Inhibiting the NF-κB Pathway in RAW264.7 Macrophages. PLoS ONE 19(5): e0304894. https://doi.org/10.1371/journal.pone.0304894

Published: May 30, 2024

Copyright: © 2024 Kumase et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.