Figures
Abstract
The pandemic of COVID-19 is a severe threat to human life and the global economy. Despite the success of vaccination efforts in reducing the spread of the virus, the situation remains largely uncontrolled due to the random mutation in the RNA sequence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which demands different variants of effective drugs. Disease-causing gene-mediated proteins are usually used as receptors to explore effective drug molecules. In this study, we analyzed two different RNA-Seq and one microarray gene expression profile datasets by integrating EdgeR, LIMMA, weighted gene co-expression network and robust rank aggregation approaches, which revealed SARS-CoV-2 infection causing eight hub-genes (HubGs) including HubGs; REL, AURKA, AURKB, FBXL3, OAS1, STAT4, MMP2 and IL6 as the host genomic biomarkers. Gene Ontology and pathway enrichment analyses of HubGs significantly enriched some crucial biological processes, molecular functions, cellular components and signaling pathways that are associated with the mechanisms of SARS-CoV-2 infections. Regulatory network analysis identified top-ranked 5 TFs (SRF, PBX1, MEIS1, ESR1 and MYC) and 5 miRNAs (hsa-miR-106b-5p, hsa-miR-20b-5p, hsa-miR-93-5p, hsa-miR-106a-5p and hsa-miR-20a-5p) as the key transcriptional and post-transcriptional regulators of HubGs. Then, we conducted a molecular docking analysis to determine potential drug candidates that could interact with HubGs-mediated receptors. This analysis resulted in the identification of top-ranked ten drug agents, including Nilotinib, Tegobuvir, Digoxin, Proscillaridin, Olysio, Simeprevir, Hesperidin, Oleanolic Acid, Naltrindole and Danoprevir. Finally, we investigated the binding stability of the top-ranked three drug molecules Nilotinib, Tegobuvir and Proscillaridin with the three top-ranked proposed receptors (AURKA, AURKB, OAS1) by using 100 ns MD-based MM-PBSA simulations and observed their stable performance. Therefore, the findings of this study might be useful resources for diagnosis and therapies of SARS-CoV-2 infections.
Citation: Sarker B, Rahaman MM, Islam MA, Alamin MH, Husain MM, Ferdousi F, et al. (2023) Identification of host genomic biomarkers from multiple transcriptomics datasets for diagnosis and therapies of SARS-CoV-2 infections. PLoS ONE 18(3): e0281981. https://doi.org/10.1371/journal.pone.0281981
Editor: Chandrabose Selvaraj, Alagappa University, INDIA
Received: October 6, 2022; Accepted: February 5, 2023; Published: March 13, 2023
Copyright: © 2023 Sarker et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper and its Supporting Information files.
Funding: This work was supported by the Ministry of Science and Technology Research Project (Ref: 505-ID, 2021–2022), Government of Bangladesh. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), a highly contagious virus, has resulted in significant loss of human life. It first emerged in Wuhan, Hubei, China in December 2019, and rapidly spread throughout the world. The World Health Organization (WHO) has declared this outbreak a pandemic for the human community [1]. The global healthcare system has been tarnished by this pandemic. As per the WHO report, as of 23 September 2022, there have been 6,512,438 reported fatalities out of a total of 611,421,786 confirmed SARS-CoV-2 infections worldwide. Clinical investigations characterized SARS-CoV-2 infections as acute respiratory tract infections with versatile symptoms, including fever, cough, fatigue, shortness of breath and pneumonia [2]. Despite the fact that the symptoms of SARS-CoV-2 infections are almost known, preventive cures for SARS-CoV-2 infections are not yet at a satisfactory level [3–6]. Early detection of SARS-CoV-2 infections and its treatment with effective drugs may play a vital role to control its outspread [7,8]. Despite the availability of a variety of vaccines against SAR-CoV-2, including those from Pfizer, CoronaVac, BBIBP-CorV, AstraZeneca, BBV152, Moderna, Sputnik, EpiVacCorona, Ad5-nCoV, and WIBP [1,2], scientists and virologists around the world are anxious yet about their effectiveness due to the unstable virus RNA sequence patterns. So, they are continuing their research to understand the molecular mechanism of SARS-CoV-2 infections more clearly for finding effective cures. SARS-CoV-2 infections are developed with the mechanisms of genetic factors and host immune responses [9–11]. Thus, exploring the significant genomic biomarkers, underlying pathogenetic mechanisms and associated drug agents may hold the potential to provide a comprehensive understanding of SARS-CoV-2 infections, and ultimately leading to the discovery of efficacious diagnostic and therapeutic strategies.
Diseases-causing genes are widely used to explore pathogenetic processes and effective drug molecules. Several individual studies explored SARS-CoV-2 infections causing host genomic biomarkers, and their pathogenetic processes based on a single transcriptomics dataset [5,6,12–16]. We reviewed their articles and did not find any common infection-causing genes. Nevertheless, difficulties may arise during the plan to take standard treatment for all against infections of SARS-CoV-2 based on their infection-causing uncommon gene-guided drugs. Therefore, more representative SARS-CoV-2 infections causing genes must be explored for diagnosis and therapies.
Advanced high-throughput technologies are now producing large-scale transcriptome data (RNA-Seq and microarray). So, it has required novel procedures to figure out the consequential information. Integrated bioinformatics and statistical approaches are widely used to develop a novel pipeline for selecting more representative diseases causing genes [17,18]. Weighted gene co-expression network analysis (WGCNA) and robust rank aggregation (RRA) are two powerful cross-validation procedures for exploring the unseen interaction between insight of gene modules and gene samples [19–21]. Therefore, in this study, an attempt was made to explore (i) more representative SARS-CoV-2 infections causing key genes from a transcriptomics profile by cross-validation with the other two independent transcriptomics profiles, (ii) pathogenetic processes and regulatory components of key genes and (iii) key genes guided potential candidate drug agents for the treatment against infections of SARS-CoV-2.
Materials and methods
This study analyzed three transcriptomics datasets and associated meta-data on SARS-CoV-2 infections that are freely available in online sources by using integrated statistics and bioinformatics approaches. The workflow of this study is displayed in Fig 1 and described in the following sections.
Dataset acquisition and preprocessing
In this study, two RNA-Seq count datasets (GSE152418 and GSE147507), and one microarray dataset (GSE152075) of SARS-CoV-2 (COVID-19) were downloaded from the publicly available gene expression omnibus (GEO) database. GSE152418 raw count data contained 17 COVID-19 and 17 healthy samples [14,22]. GSE147507 raw count data contained 6 samples (3 COVID-19 patients and 3 NHBE samples) [15,23]. GSE152075 is a microarray data containing 430 COVID-19 infected samples and 54 negative samples [16,24–26]. The microarray dataset GSE152075 was downloaded using the Bioconductor package GEOquery, and the batch effect of this dataset was removed by using the R package sva via Combat_seq [27,28]. GSE152418 is used as a discovery dataset analyzed by WGCNA, GSE147507 is used as an independent validation, and GSE152075 is used as a test dataset. In the COVID-19 datasets, genes that comprise only zero counts have been removed.
Identification of differentially expressed genes (DEGs)
Differentially expressed genes (DEGs) were identified from the two RNA-Seq count datasets (GSE152418 and GSE147507) through edgeR R-package [16], and a microarray dataset (GSE152075) through limma R-package [29]. Genes were selected as DEGs that satisfy the criteria of adjusted P-value (Benjamini-Hochberg) < 0.05 and |log2 (FC) | ≥ 1.
Weighted gene co-expression network analysis (WGCNA) with DEGs
The WGCNA approach was used for exploring modules (clusters) of highly correlated DEGs, summarizing such modules using the cluster eigengene or an intracluster hub genes, relating clusters to each other and to external sample traits (using eigengene networking), and for detecting cluster membership. We implemented this approach using the WGCNA R package [30]. In WGCNA, the pickSoftThreshold function was used for fitting soft-thresholding powers β over the value of maximum R2. Then adjacency matrix and Topological Overlap Matrix (TOM) were created using TOM similarity. The dissimilarity of TOM (dissTOM) was computed using dissimilarity modules. Modules constructions of DEGs were performed using the hclust function from the dissTOM based dynamic cut tree (dendrogram). Different parameters were used for preventing large and small modules i.e., medium sensitivity (deepSplit = 2) and minimum module size (minClusterSize = 30). Module eigengene (ME) was used for merging similar modules based on MEDissThres = 0.25 function.
Module analysis for validation of DEGs
To find the significant module of co-expressed DEGs (obtained through the WGCNA by integration of test dataset GSE152075), the module preservation function was used. The module preservation function is used to identify whether a module is reproducible and robust across the datasets or not [31]. We considered the module to be preserved if the statistic satisfied above Z summary > 10. It is specified negative correlation between preservation statistic-median rank and module preservation, and there is a positive correlation between the module preservation and Z summary statistic. Then the host DEGs were identified based on 2-fold cross-validation namely module membership statistic (MMS) calculated by the Pearson’s correlation and Protein-protein interaction networks (PPIN). Genes of MMS, PPIN and DEGs from independent datasets were chosen as host signatures by RRA. The final subsets of host hub DEGs were separated by PPIN analysis (Fig 1).
Protein-protein interaction (PPI) network analysis based on validated DEGs
We performed PPI network analysis to explore SARS-CoV-2 infection causing hub-genes. To construct the PPI network for host signatures, genes data were collected from the STRING database [32], and the Cytoscape software [33] was used to construct the network based on the parameter: confidence score ≥ 0.4 and most extreme interactors = 0 for cutoff models. Similarly, the hub-genes signatures were separated. After that, these hub signatures were used for gene enrichment analysis, finding transcriptional and post transcriptional regulators and drug repurposing with molecular docking analysis described in the next section.
Functional enrichment analysis of hub-genes
The Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), WikiPathways and BioCarta pathway enrichment analyses for hub-genes were performed via the web-based tool Enrichr [34] to explore the pathogenetic processes of SARS-CoV-2 infections. P-value (Adjusted) < 0.05 was used to extract the significant biological information.
Hub-genes regulatory network analysis
To explore transcriptional and post-transcriptional regulators of hub-genes, we performed transcription factors (TFs) versus hub genes and micro RNAs versus hub genes interaction by using the databases TF2DNA [35] and miRDB [36], respectively.
Meta-data collection
We collected 177 drug agents as a meta-data from the literature review of 16 COVID-19 related articles to explore the potential candidate drugs (S1 Table). To validate the proposed repurposed candidate drugs by using molecular docking (MD) analysis with the top-ranked receptor proteins associated with COVID-19, the metadata were obtained from the literature review (S2 Table). We selected top-ranked COVID-19 associated 8 receptor proteins as meta-data by reviewing 24 newly published articles to assess the binding affinity of the proposed candidate drugs with these receptor proteins (S2 Table).
Molecular docking
To explore repurposable effective drug molecules for COVID-19 by in-silico validation, molecular docking analysis was performed between the target proteins and meta-drug agents. Our proposed HubGs mediated proteins and their associated TFs proteins were considered the drug target receptors, and 177 meta-drugs as the drug-agents that were obtained from the literature review and other sources as mentioned earlier in the data sources (S1 Table). From Protein Data Bank (PDB) [37] and SWISS-MODEL [38], the 3-Dimensional (3D) structures of receptor proteins were downloaded. The PubChem database [39] was used to download the 3D structures of drug agents. The PyMOL 2.4.1 software was used to visualize the 3D structure of the target receptor proteins [40]. The protein chains which were not a part of the gene are deleted [41]. Then, Swiss PDB viewer software was used to add charges and minimize the energy of the target proteins [42]. The target proteins were prepared for molecular docking analysis by eliminating water molecules and ligand heteroatoms, adding polar hydrogens, and converting them to pdbqt format using AutoDock tools 1.5.7 [43]. Avogadro software was used for minimizing the energy of the ligands [44]. The ligands were prepared for dynamic simulation by setting the torsion tree and rotatable, and nonrotatable bonds present in the ligand through AutoDock tools 1.5.7 [43]. Then, the binding affinities score between the ligand and receptors were calculated by using AutoDock Vina [45]. The Discovery Studio Visualizer 2019 was used to analyze the docked complexes. Let Sij indicates the binding score of ith receptors (i = 1, 2, …, m) with the jth ligand (j = 1, 2,…, n). Then receptors were ordered according to the decreasing order of row means and ligands were ordered according to the decreasing order of column means
to select the top-order ligands as the candidate drug agents [5,17,46].
Molecular Dynamics (MD) simulation
To perform the dynamic properties of top-ordered protein-ligand complexes, YASARA [47] and the AMBER14 force field [48] were used in Molecular Dynamics (MD) simulations. We assigned the ligands parameters for the complexes by using AutoSMILES [49] algorithms, which automatically parameterize unknown organic molecules by computing semi-empirical AM1 Mulliken point charges with the COSMO solvation model, assigning AM1BCC [50] atom and bond types, and assigning general AMBER force field (GAFF) [51] atom types, and the remaining parameters of force field. In a simulation cell, the hydrogen bonding network of protein-ligand complexes were optimized and solvated by a TIP3P [52] water model before the simulation. We considered the solvent density of 0.997 g L-1 to maintain the periodic boundary conditions. During solvation, titratable amino acids in the protein complex were assigned to calculate pKa. The initial energy minimization process of each simulation system, consisting of 53735, 54335, and 79559 atoms for AURKA vs. Nilotinib, AURKB vs. Tegobuvir, and OAS1 vs. Proscillaridin complexes was performed by a simulated annealing method, respectively using the steepest gradient approach (5000 cycles).
A multiple-time-step algorithm [53] with 2.50 fs time step interval under physiological conditions (298 K, pH 7.4, 0.9% NaCl) [54] was used to run the simulation of each complex. The linear constraint solver (LINCS) [55] algorithm was used to constrain all bond lengths, and SETTLE [56] was employed to control the water molecules. PME methods [57] were used to describe long-range electrostatic interactions, and 100 ns MD simulation was performed at Berendsen thermostat [58] and constant pressure. The trajectories were captured at every 250 ps for further analysis, and subsequent analysis was performed by the built-in script of the YASARA [59] macro and SciDAVis software (http://scidavis.sourceforge.net/). All the captured snapshots were used to calculate MM-Poisson–Boltzmann Surface Area (MM-PBSA) binding free energy by YASARA software using the formula below [60]:
Here, we computed MM-PBSA binding energy by YASARA default macros using AMBER 14 as a force field, with larger positive energies indicating better binding [61].
Results
Identification of DEGs
Two different raw RNA-Seq datasets (GSE152418 and GSE147507) and one microarray gene expression profile (GSE152075) were used for differential expression analysis (Table 1). We identified 2389 DEGs with 636 up-regulated and 1753 down-regulated genes for the GSE152418 dataset, and 540 DEGs with 213 up-regulated and 327 down-regulated genes for the GSE147507 dataset. We also identified 2102 DEGs with 570 up-regulated and 1532 down-regulated genes for GSE152075. These identified DEGs are used for further analysis.
WGCNA analysis for validation of DEGs
WGCNA was performed with the DEGs in the dataset GSE152418. The power value is the most important parameter which is contaminated with the average connectivity degree (ACD) and independence of co-expression modules (ICEM). The power value (Fig 2A) shows that ACD was greater, and the value of ICEM reached the expected value 0.8. Thus, the power value is ready to create the co-expression module and constructed with multiple colors presented in Fig 2B. The co-expression network constructed seven modules, namely black, red, yellow, turquoise, green, brown and blue, based on the soft threshold power β = 6 with R2 = 0.80. The Eigengene dendrogram and eigengene network heatmap represent the interactions among the co-expression modules (Fig 2C). Then the dataset GSE152418 was compared with the test dataset GSE152075, and the summary of preservation statistic was visualized (Fig 2D). We observed that among the seven modules black, red, yellow and turquoise are the most stable modules (Zsummary statistic: above Z = 2 and Z = 10). The remaining modules were considered nonstable (Z summary statistic < 10). Black, red, yellow, and turquoise colors showed minimum median rank statistic which indicated that their preservation is best than the other modules.
(A) Illustration of soft-thresholding powers based on the scale-free tropology model fit (left) and the mean connectivity (right). (B) The dendrogram of all DEGs clustered based on a dissimilarity measure (1-TOM). (C) The dendrogram of eigengene module and cluster analysis of eigengene network by heatmap summarize the modules yielded in the clustering analysis. (D) Median rank preservation (left) and Z summary preservation (Right); the black, red, yellow and turquoise indicate the strong preservation above dashed lines Z = 2 and Z = 10.
Identification of hub-genes from validated DEGs
We identified 475 significant genes using high connectivity modules black, red, yellow and turquoise through the threshold |cor.geneModuleMembership| > 0.8. Again, the PPI network extracted 658 significant genes based on the highest degree > 9 for the four modules. These two gene sets were validated using the validation DEGs set. Validation DEGs set obtained from the GSE147507 dataset to confirm the most stable gene set of COVID-19. We used RRA to identify the top 50 significant genes in this case. Finally, eight hub genes (REL, AURKA, AURKB, FBXL3, OAS1, STAT4, MMP2 and IL6) are identified from the top 50 genes through the PPI network analysis (Fig 3).
Functional enrichment analysis of hub-genes
Various pathway enrichment analyses were performed to explore further biological insight of the HubGs. GO and pathway terms with P-value (adjusted) < 0.05 were considered significant. The information of GO with their three subsections (BPs, MFs, CCs) is presented in Table 2. The significant BPs are mainly enriched in the negative regulation of chemokine production, liver development and response to peptide, etc. The significant MFs enriched in the histone serine kinase activity, histone kinase activity, interleukin-6 receptor binding, etc. The significant CCs are enriched in the spindle microtubule, condensed chromosome, microtubule, etc. Different pathways; KEGG, WikiPathways, and BioCarta analysis results are presented in Table 3. The KEGG pathways for the hub genes are enriched in several pathways such as inflammatory bowel disease, AGE-RAGE signaling pathway in diabetic complications, pathways in cancer and coronavirus disease, etc. The WikiPathways pathway analysis results enhanced in Photodynamic therapy-induced NF-kB survival signaling WP3617, FOXP3 in COVID-19 WP5063, COVID-19 adverse outcome pathway WP4891, STING pathway in Kawasaki-like disease and COVID-19 WP4961, and Host-pathogen interaction of human coronaviruses—interferon induction WP4880, etc. BioCarta is mostly involved in Interleukin-27-mediated signaling events, FRA pathway, Interleukin-23-mediated signaling events and so on.
Hub-genes regulatory network analysis
We identified SRF, PBX1, MEIS1, ESR1 and MYC hub-TFs (Fig 4A), and hsa-miR-106b-5p, hsa-miR-20b-5p, hsa-miR-93-5p, hsa-miR-106a-5p and hsa-miR-20a-5p hub-miRNAs (Fig 4B) from the TFs-HubGs and miRNA-HubGs interaction network, respectively.
Hub-genes regulatory network analysis with (A) Transcription factors (TFs) and (B) micro RNAs (miRNAs).
Exploring candidate drugs by molecular docking simulation
We considered HubGs mediated 8 proteins (REL, AURKA, AURKB, FBXL3, OAS1, STAT4, MMP2 and IL6) and their regulatory 5 hub-TFs proteins (SRF, PBX1, MEIS1, ESR1 and MYC) as the receptor proteins. The 3D structure of REL, AURKA, AURKB, FBXL3, OAS1, STAT4, MMP2, IL6, SRF, PBX1, MEIS1, ESR1 and MYC; targets were downloaded from PDB (Protein Data Bank) using the codes 1a3q, 6gra, 3af3, 4i6j, 4ig8, 4gj2, 3ayu, 5fuc, 1srs, 1puf, 5ego, 1uom, and 6e16, respectively. Then we considered 177 drug molecules (drug agents) that were selected by the literature review of COVID-19 related articles (S1 Table) and downloaded their 3D structures from the PubChem database. Then we performed molecular docking analysis of each receptor with each agent.
Fig 5A displayed the binding affinity score matrix between the ordered receptors and drug agents. We observed that each of the top 10 lead compounds (Nilotinib, Tegobuvir, Digoxin Proscillaridin, Olysio, Simeprevir, Hesperidin, Oleanolic Acid, Naltrindole and Danoprevir) produces binding affinity scores less than or equal to -7.0 kcal/mol with all of our suggested receptors (Section-I in S1 File for score matrix). Therefore, we considered these 10 drugs as the candidate drug agents in this study. To validate the proposed drugs against the state-of-the-art alternative independent receptors, we considered the top-ranked 8 hub-genes (CASP3, CXCL8, ICAM1, IL6, NFKBIA, STAT1, TNF and IRF7) that are common in at least 3 articles (S2 Table) out of 24. The 3D structures of these 8 independent receptor proteins were downloaded from Protein Data Bank (PDB) with codes 4ps0, 6n2u, 5mza, 5fuc, 1nfi, 1bf5, and 7kba, and receptor protein IRF7 were retrieved from the SWISS-MODEL using the UniProt IDs Q92985, respectively. Fig 5B represents the binding affinities (kcal/mol) between the proposed drugs and publicly available top-ranked independent receptors. We observed that three lead compounds (lead1: Tegobuvir, lead2: Nilotinib, lead3: Proscillaridin) strongly bind with all independent receptors (Section-II in S1 File for score matrix). Table 4 represents the summary results of interacting properties of the top targets with top-ranked lead compounds that produced highest binding scores. We also examined their complete interaction profile including hydrophobic, hydrogen bonds, and electrostatic interactions. We illustrated 2D structure of proteins and ligands interaction in Fig 6. The 3D structure of their interacting complex and top-ranked lead compounds are shown in the Fig 7. To investigate the stability of the top three complexes, we performed molecular dynamic simulations as discussed in the next section.
(A) Row indicates ordered 13 proposed receptor proteins and column indicates the top-ordered 50 drug agents out of 158, where red colors indicate the strong binding affinities, (B) Row indicates top-ranked 8 receptor proteins obtained through published literature, and column indicates the proposed top-ordered 10 drug agents out of 158, where red colors indicate the strong binding affinities.
(A) AURKA vs. Nilotinib, (B) AURKB vs. Tegobuvir, and (C) OAS1 vs. Proscillaridin.
Lead compound (left side) and three complexes of three-dimensional chemical interactions (right side) obtained from molecular docking. (a) AURKA vs. Nilotinib, (b) AURKB vs. Tegobuvir, and (c) OAS1 vs. Proscillaridin.
The last row shows key interactions of amino acids and their binding types with potential targets.
Molecular Dynamic (MD) simulations
Three predicted drug agents (Nilotinib, Tegobuvir, and Proscillaridin) showed the highest binding affinities with AURKA, AURKB, and OAS1 proteins, respectively (Table 4). Therefore, three complexes (AURKA vs. Nilotinib, AURKB vs. Tegobuvir, and OAS1 vs. Proscillaridin) were considered for stability analysis using 100 ns MD-based MM-PBSA simulations. We observed that these 3 complexes (AURKA vs. Nilotinib, AURKB vs Tegobuvir, OAS1 vs. Proscillaridin) showed significant stability between the variations of moving and initial drug-target protein complexes (Fig 8A). RMSD values corresponding to each complex were calculated. All the systems projected the RMSD values around 1.5 Å to 4.15 Å. The average RMSD values for AURKA vs. Nilotinib, AURKB vs. Tegobuvir, and OAS1 vs. Proscillaridin complexes were 2.543 Å, 2.863 Å, and 2.324 Å, respectively. The OAS1 vs. Proscillaridin complex displayed a more rigid conformation than the other complexes, reached equilibrium at 35 ns, and remained almost stable, after that AURKA vs. Nilotinib complex showed almost stable performance during 10 ns to 35 ns, 52 ns to 70 ns and the remaining times there were irregular fluctuations in the RMSD. On the contrary, AURKB vs. Tegobuvir complexes exhibited irregular fluctuation and RMSD values fluctuate from 2.0 Å to 4.15 Å over the time period. In addition, MM-PBSA binding energy for three complexes were also calculated, and Fig 8B illustrated the binding energies of the complexes. On average, AURKA vs. Nilotinib, AURKB vs. Tegobuvir, and OAS1 vs. Proscillaridin complexes produced MM-PBSA binding energies 58.5 kcal/mol, 53.92 kcal /mol, and– 6.834 kcal /mol, respectively.
(A) Time evolution of RMSDs for each of the top-ranked three complexes. (B) Binding free energy (kcal/mol) of each snapshot was calculated by MM-PBSA, representing the change in binding stability of each complex during simulations; positive values indicate better binding. Complexes: blue AURKA vs. Nilotinib, red AURKB vs. Tegobuvir, and green. OAS1 vs. Proscillaridin.
Discussion
COVID-19 is the most recent and ongoing pandemic that has adversely affected on human health and the world’s economy. Though vaccination programs were started globally at a marginal rate, it is still a threat to public health. Gene signatures are the pathological indicator for describing diseases at a molecular level. In this study, we used bioinformatics approach to detect gene signatures and potential therapeutic drugs for the treatment of COVID-19 patients. The present study employed three different datasets (Table 1) to identify potential DEGs between COVID-19 and control samples. The results of the analysis revealed a total of 2389 and 540 DEGs from two RNA-Seq datasets and 2102 DEGs from the microarray dataset. To select the potential DEGs, we validated these 3 DEGs-sets by WGCNA, PA and RRA procedures. Then we selected top-ranked 50 DEGs as the most potential DEGs. We performed protein-protein interaction (PPI) network analysis of those 50 DEGs to select the HubGs. Finally, we selected top-ranked 8 DEGs (REL, AURKA, AURKB, FBXL3, OAS1, STAT4, MMP2, IL6) as the HubGs (Fig 3), that were used for further investigation of SARS-CoV-2 infections. The literature review also supported these HubGs as the SARS-CoV-2 infection-causing genes (Fig 9A). As for example, the gene REL has been previously reported as a hub gene for SARS-CoV-2 infections [16]. By combining some studies, we found that the gene AURKA is a common targeted protein for both COVID-19 and lung adenocarcinoma patients [14,17,62]. The gene AURKB plays a crucial role as a biomarker gene in the diagnosis and prognosis of COVID-19 patients [14]. The gene FBXL3 has been identified as a core gene of COVID-19 [14,63–65]. It has been noted that the gene OAS1 is an important gene influencing COVID-19 patients [66–70]. The gene STAT4 is the human transcriptomic factor of COVID-19 [71–73]. The gene MMP2 has been recognized as a hub gene in COVID-19-infected patients [13,74]. The gene IL-6 can safeguard against basic circumstances with coronavirus, diminishing IL-6 articulation [75–83]. The interaction network analysis between HubGs and transcription factors (TFs) revealed the top-ranked 5 TFs genes (SRF, PBX1, MEIS1, ESR1 and MYC) as the key transcriptional regulators of HubGs (Fig 4A). Notably, the SRF gene demonstrated a unique and dysfunctional pattern in COVID-19 [5,6,84,85]. The TF genes PBX1 has been found to possess multiple functions relevant to cell development and has been associated with tumor agents and COVID-19 [16,86], MEIS1 has been identified as the targeted agent of SARS-CoV-2 [16,87], ESR1 has been noted to act as an antiviral signature that disrupts the viral membrane of the SARS-CoV-2 protein [88]. Furthermore, MYC is another target gene of COVID-19, has been reported to have various functions, including regulation of chromatin sites, modulation of cellular metabolism, and versatility across various cell types [87,88]. The hub-genes versus micro-RNA interaction network analysis revealed top-ranked 5 miRNAs (hsa-miR-106b-5p, hsa-miR-20b-5p, hsa-miR-93-5p, hsa-miR-106a-5p and hsa-miR-20a-5p) as the post-transcriptional regulators of hub-genes (Fig 4B). The miRNA, hsa-miR-106b-5p, has been identified as a tumor promoter and targeted receptor for different cancers [89]. The hsa-miR-20b-5p miRNA has been shown to play an antiviral role in patients infected with SARS-CoV and SARS-CoV-2, as well as the up-regulated signature of the influenza virus [90]. The hsa-miR-93-5p miRNA is associated with human cancerous growth and encourages angiogenic operation [91]. The miRNA, hsa-miR-106a-5p, promotes virus mechanism of COVID-19 [92]. The miRNA, hsa-miR-20a-5p has been shown to play a significant role in respiratory viruses including adenovirus 2, influenza A and RSV [93].
(A) Validation of the proposed HubGs: circles with node color indicates hub genes, and each connected network with number(s) indicates the reference(s) of gene(s) of SARS-CoV-2 (B) Validation of the proposed candidate drugs: circles with green color indicate FDA approved, light blue color indicates investigational drugs and purple color indicates experimental drugs and red color indicates unapproved drugs, and each connected network with number(s) indicates the reference(s) of drug(s) of SARS-CoV-2.
To explore the biological insights underlying HubGs we used web-based tool Enrichr. Pathological information of HubGs described the significance of biomarker agents by using gene ontology and pathway analysis. GO analysis enriched with the regulation of acute inflammatory response [11], interleukin-6 receptor binding [94], and Intracellular membrane-bounded organelle [95] (Table 2). KEGG pathway associated with influenza A, coronavirus disease [96], bladder cancer and malaria. WikiPathways Interferon type I signaling pathways WP585, FOXP3 in COVID-19 WP5063, COVID-19 adverse outcome pathway WP4891, STING pathway in Kawasaki-like disease and COVID-19 WP4961 (Table 3). To find the effective drug molecules against COVID-19, we used the proposed 8 target proteins and their regulatory 5 key TFs proteins as the receptor proteins. We performed their docking analysis with 177 meta-drug agents (S1 File).
Then we picked up the top-ranked 10 drugs (Nilotinib, Tegobuvir, Digoxin, Proscillaridin, Olysio, Simeprevir, Hesperidin, Oleanolic Acid, Naltrindole, and Danoprevir) as the candidate drug agents based on their strong binding affinities with all the target proteins (Fig 5A). These drug molecules are also supported by other individual studies for the treatment against SARS-CoV-2 infections which includes Nilotinib [97–102], Tegobuvir [103–109], Digoxin [110–115], Proscillaridin [116–121], Olysio [109,122,123], Simeprevir [119,124–128], Hesperidin [129–133], Oleanolic Acid [133–136], Danoprevir [6,114,137–143], Naltrindole [144] for the treatment against COVID-19 (Fig 9B). Fig 5B displays the results of cross-validation of our suggested ten candidate drug agents with the top-ranked independent receptor proteins, and observed their strong binding affinities. Finally, the binding stability of the top three complexes (AURKA vs. Nilotinib, AURKB vs. Tegobuvir, and OAS1 vs. Proscillaridin) were investigated by molecular dynamics (MD) based MM-PBSA simulations, which revealed their stable performance (Fig 8) [145,146].
The phylogenetic tree and pairwise alignment results on identities, similarities and gaps of HubGs (REL, AURKA, AURKB, FBXL3, OAS1, STAT4, MMP2 and IL6) protein sequences showed that AURKA and AURKB proteins are more-closer to each other with largest identity (54.3%) and similarity (63.4%) and, smallest gap (28.3%) compares to any other pair of HubGs (S2 File for MSA, phylogenetic tree, identity, similarity, score and gaps). The binding affinity scores of these two proteins were found significantly larger and almost same with respect to our suggested drug molecules (Fig 5A). On the other hand, we also observed that proteins OAS1 and FBXL3 are second more-closer to each other with larger identity (28.7%) and similarity (37.0%) and, smaller gap (52.7%) compares to any other pair of the rest HubGs. The binding affinity scores for these two proteins were also larger and almost similar against our suggested drug molecules. The MD simulation-based MM-PBSA analysis showed the average binding free energy for AURKA and AURKB are almost similar (58.5 kcal/mol & 53.92 kcal/mol) but far different from OAS1 (-6.834 kcal/mol). Thus, the molecular signatures and potential repurposable drug agents that we have identified in this study may serve as valuable resources for wet-lab validation and the development of an effective treatment plan against SARS-CoV-2 infections.
Conclusions
This study suggested SARS-CoV-2 infection causing core genes (REL, AURKA, AURKB, FBXL3, OAS1, STAT4, MMP2 and IL6) by highlighting their key transcriptional regulators (SRF, PBX1, MEIS1, ESR1 and MYC) and post-transcriptional regulators (hsa-miR-106b-5p, hsa-miR-20b-5p, hsa-miR-93-5p, hsa-miR-106a-5p and hsa-miR-20a-5p). To explore the effective drugs for SARS-CoV-2 infections by the molecular docking analysis, core gene mediated proteins and five TFs proteins were considered as the receptors. Based on our computational analysis, we nominated top-ranked 10 candidate drugs (Nilotinib, Tegobuvir, Digoxin, Proscillaridin, Olysio, Simeprevir, Hesperidin, Oleanolic Acid, Naltrindole, and Danoprevir) that showed the highest docking scores, indicating their favorable binding affinity with the receptors. Then we validated the suggested drug molecules against the state-of-the-art alternatives publicly available top-ranked 8 independent receptors (CASP3, CXCL8, ICAM1, IL6, NFKBIA, STAT1, TNF and IRF7) by molecular docking and found their significant binding affinities. Finally, we examined the stability of top-ranked three receptor-ligand complexes (AURKA vs. Nilotinib, AURKB vs. Tegobuvir, OAS1 vs. Proscillaridin) by computing the RMSD scores and binding free energies through the 100 ns MD-simulation based MM-PBSA approach, and observed their stable performance. In this regard, this study might open up a new gateway to explore more effective drug molecules computationally against SARS-CoV-2 infections. Thus, the outputs of this study might be useful inputs for wet-lab experiment to make a proper treatment plan against SARS-CoV-2 infections.
Supporting information
S1 Table. Collection of 177 meta drug agents by literature review.
https://doi.org/10.1371/journal.pone.0281981.s001
(DOCX)
S2 Table. Targeted protein list from different published literature.
https://doi.org/10.1371/journal.pone.0281981.s002
(DOCX)
S1 File.
(I) Binding score of the interaction of targeted proteins with targeted drugs. (II) Binding score of the interaction of published proteins with proposed drugs and published drugs.
https://doi.org/10.1371/journal.pone.0281981.s003
(XLSX)
S2 File.
(I) Multiple Sequence Alignment (MSA) Results for HubGs. (II) Identity, Similarity, Gap and Score matrices based on the alignment results of HubGs. (III) Phylogenetic tree of HubGs.
https://doi.org/10.1371/journal.pone.0281981.s004
(XLSX)
Acknowledgments
We are thankful to the reviewers for his/her help in improving the quality of the manuscript.
References
- 1. Cucinotta D, Vanelli M. WHO declares COVID-19 a pandemic. Acta Bio Medica: Atenei Parmensis. 2020;91: 157. pmid:32191675
- 2. Chen N, Zhou M, Dong X, Qu J, Gong F, Han Y, et al. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. The lancet. 2020;395: 507–513. pmid:32007143
- 3. Cao B, Wang Y, Wen D, Liu W, Wang J, Fan G, et al. A trial of lopinavir–ritonavir in adults hospitalized with severe Covid-19. New England Journal of Medicine. 2020.
- 4. Zhou P, Yang X-L, Wang X-G, Hu B, Zhang L, Zhang W, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. nature. 2020;579: 270–273. pmid:32015507
- 5. Mosharaf MdP Reza MdS, Kibria MdK Ahmed FF, Kabir MdH Hasan S, et al. Computational identification of host genomic biomarkers highlighting their functions, pathways and regulators that influence SARS-CoV-2 infections and drug repurposing. Sci Rep. 2022;12: 4279. pmid:35277538
- 6. Ahmed FF, Reza MdS, Sarker MdS, Islam MdS, Mosharaf MdP, Hasan S, et al. Identification of host transcriptome-guided repurposable drugs for SARS-CoV-1 infections and their validation with SARS-CoV-2 infections by using the integrated bioinformatics approaches. Ashfaq UA, editor. PLoS ONE. 2022;17: e0266124. pmid:35390032
- 7.
D’Orazio M, Bernardini G, Quagliarini E. A probabilistic model to evaluate the effectiveness of main solutions to COVID-19 spreading in university buildings according to proximity and time-based consolidated criteria. Building simulation. Springer; 2021. pp. 1795–1809.
- 8. Kitchin R. Civil liberties or public health, or civil liberties and public health? Using surveillance technologies to tackle the spread of COVID-19. Space and Polity. 2020;24: 362–381.
- 9. Mohammadpour S, Torshizi Esfahani A, Halaji M, Lak M, Ranjbar R. An updated review of the association of host genetic factors with susceptibility and resistance to COVID-19. Journal of Cellular Physiology. 2021;236: 49–54. pmid:32542735
- 10. Zhang X, Tan Y, Ling Y, Lu G, Liu F, Yi Z, et al. Viral and host factors related to the clinical outcome of COVID-19. Nature. 2020;583: 437–440. pmid:32434211
- 11. Tay MZ, Poh CM, Rénia L, MacAry PA, Ng LF. The trinity of COVID-19: immunity, inflammation and intervention. Nature Reviews Immunology. 2020;20: 363–374. pmid:32346093
- 12. Yuan D, Zhou H, Sun H, Tian R, Xia M, Sun L, et al. Identification of key genes for guiding chemotherapeutic management in ovarian cancer using translational bioinformatics. Oncology letters. 2020;20: 1345–1359. pmid:32724377
- 13. Nan KS, Karuppanan K, Kumar S. Identification of common key genes and pathways between Covid-19 and lung cancer by using protein-protein interaction network analysis. Bioinformatics; 2021 Feb.
- 14. Auwul MR, Rahman MR, Gov E, Shahjaman M, Moni MA. Bioinformatics and machine learning approach identifies potential drug targets and pathways in COVID-19. Briefings in Bioinformatics. 2021;22: bbab120. pmid:33839760
- 15. Karami H, Derakhshani A, Ghasemigol M, Fereidouni M, Miri-Moghaddam E, Baradaran B, et al. Weighted Gene Co-Expression Network Analysis Combined with Machine Learning Validation to Identify Key Modules and Hub Genes Associated with SARS-CoV-2 Infection. Journal of clinical medicine. 2021;10: 3567. pmid:34441862
- 16. Vastrad B, Vastrad C, Tengli A. Bioinformatics analyses of significant genes, related pathways, and candidate diagnostic biomarkers and molecular targets in SARS-CoV-2/COVID-19. Gene Reports. 2020;21: 100956. pmid:33553808
- 17. Reza MdS Harun-Or-Roshid Md, Islam MdA Hossen MdA, Hossain MdT, Feng S, et al. Bioinformatics Screening of Potential Biomarkers from mRNA Expression Profiles to Discover Drug Targets and Agents for Cervical Cancer. IJMS. 2022;23: 3968. pmid:35409328
- 18. Alam MS, Rahaman MM, Sultana A, Wang G, Mollah MNH. Statistics and network-based approaches to identify molecular mechanisms that drive the progression of breast cancer. Computers in Biology and Medicine. 2022;145: 105508. pmid:35447458
- 19. Liu R, Zhang W, Liu Z-Q, Zhou H-H. Associating transcriptional modules with colon cancer survival through weighted gene co-expression network analysis. BMC genomics. 2017;18: 1–9.
- 20. Jin X, Li J, Li W, Wang X, Du C, Geng Z, et al. Weighted gene co-expression network analysis reveals specific modules and biomarkers in Parkinson’s disease. Neuroscience Letters. 2020;728: 134950. pmid:32276105
- 21. Malik M, Parikh I, Vasquez JB, Smith C, Tai L, Bu G, et al. Genetics ignite focus on microglial inflammation in Alzheimer’s disease. Molecular neurodegeneration. 2015;10: 1–12.
- 22. Arunachalam PS, Wimmers F, Mok CKP, Perera RAPM, Scott M, Hagan T, et al. Systems biological assessment of immunity to mild versus severe COVID-19 infection in humans. Science. 2020;369: 1210–1220. pmid:32788292
- 23. Blanco-Melo D, Nilsson-Payant BE, Liu W-C, Uhl S, Hoagland D, Møller R, et al. Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19. Cell. 2020;181: 1036–1045.e9. pmid:32416070
- 24. Lieberman NA, Peddu V, Xie H, Shrestha L, Huang M-L, Mears MC, et al. In vivo antiviral host transcriptional response to SARS-CoV-2 by viral load, sex, and age. PLoS biology. 2020;18: e3000849. pmid:32898168
- 25. Li Y, Jiang Y, Zhang Y, Li N, Yin Q, Liu L, et al. Abnormal upregulation of cardiovascular disease biomarker PLA2G7 induced by proinflammatory macrophages in COVID-19 patients. Scientific reports. 2021;11: 1–11.
- 26. Liu J, Yuan S, Yao Y, Wang J, Scalabrino G, Jiang S, et al. Network Pharmacology and Molecular Docking Elucidate the Underlying Pharmacological Mechanisms of the Herb Houttuynia cordata in Treating Pneumonia Caused by SARS-CoV-2. Viruses. 2022;14: 1588. pmid:35891565
- 27. Leek JT, Johnson WE, Parker HS, Jaffe AE, Storey JD. The sva package for removing batch effects and other unwanted variation in high-throughput experiments. Bioinformatics. 2012;28: 882–883. pmid:22257669
- 28. Davis S, Meltzer PS. GEOquery: a bridge between the Gene Expression Omnibus (GEO) and BioConductor. Bioinformatics. 2007;23: 1846–1847. pmid:17496320
- 29.
Smyth GK, Ritchie M, Thorne N, Wettenhall J. LIMMA: linear models for microarray data. In Bioinformatics and Computational Biology Solutions Using R and Bioconductor. Statistics for Biology and Health. 2005.
- 30. Langfelder P, Horvath S. WGCNA: an R package for weighted correlation network analysis. BMC bioinformatics. 2008;9: 1–13.
- 31. Langfelder P, Luo R, Oldham MC, Horvath S. Is my network module preserved and reproducible? PLoS computational biology. 2011;7: e1001057. pmid:21283776
- 32. Szklarczyk D, Franceschini A, Wyder S, Forslund K, Heller D, Huerta-Cepas J, et al. STRING v10: protein–protein interaction networks, integrated over the tree of life. Nucleic acids research. 2015;43: D447–D452. pmid:25352553
- 33. Saito R, Smoot ME, Ono K, Ruscheinski J, Wang P-L, Lotia S, et al. A travel guide to Cytoscape plugins. Nature methods. 2012;9: 1069. pmid:23132118
- 34. Kuleshov MV, Jones MR, Rouillard AD, Fernandez NF, Duan Q, Wang Z, et al. Enrichr: a comprehensive gene set enrichment analysis web server 2016 update. Nucleic acids research. 2016;44: W90–W97. pmid:27141961
- 35. Pujato M, Kieken F, Skiles AA, Tapinos N, Fiser A. Prediction of DNA binding motifs from 3D models of transcription factors; identifying TLX3 regulated genes. Nucleic acids research. 2014;42: 13500–13512. pmid:25428367
- 36. Wong N, Wang X. miRDB: an online resource for microRNA target prediction and functional annotations. Nucleic acids research. 2015;43: D146–D152. pmid:25378301
- 37. Berman HM, Battistuz T, Bhat TN, Bluhm WF, Bourne PE, Burkhardt K, et al. The Protein Data Bank. Acta Crystallogr D Biol Crystallogr. 2002;58: 899–907. pmid:12037327
- 38. Waterhouse A, Bertoni M, Bienert S, Studer G, Tauriello G, Gumienny R, et al. SWISS-MODEL: Homology modelling of protein structures and complexes. Nucleic Acids Research. 2018;46. pmid:29788355
- 39. Kim S, Chen J, Cheng T, Gindulyte A, He J, He S, et al. PubChem 2019 update: Improved access to chemical data. Nucleic Acids Research. 2019;47. pmid:30371825
- 40.
Visualizer DS v4. 0. 100. 13345. Accelrys Sof tware Inc (2005).
- 41. Kaplan W, Littlejohn TG. Swiss-PDB Viewer (Deep View). Briefings in bioinformatics. 2001;2. pmid:11465736
- 42. El-Hachem N, Haibe-Kains B, Khalil A, Kobeissy FH, Nemer G. AutoDock and AutoDockTools for protein-ligand docking: Beta-site amyloid precursor protein cleaving enzyme 1(BACE1) as a case study. Methods in Molecular Biology. 2017. pmid:28508374
- 43. Hanwell MD, Curtis DE, Lonie DC, Vandermeerschd T, Zurek E, Hutchison GR. Avogadro: An advanced semantic chemical editor, visualization, and analysis platform. Journal of Cheminformatics. 2012;4. pmid:22889332
- 44. Trott O, Olson AJ. AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. Journal of Computational Chemistry. 2009. pmid:19499576
- 45. Krieger Elmar GV, Spronk C. YASARA—Yet Another Scientific Artificial Reality Application. YASARA.org. 2013.
- 46. Dickson CJ, Madej BD, Skjevik ÅA, Betz RM, Teigen K, Gould IR, et al. Lipid14: The amber lipid force field. Journal of Chemical Theory and Computation. 2014;10. pmid:24803855
- 47. Stewart JJP. MOPAC: A semiempirical molecular orbital program. Journal of Computer-Aided Molecular Design. 1990;4. pmid:2197373
- 48. Jakalian A, Jack DB, Bayly CI. Fast, efficient generation of high-quality atomic charges. AM1-BCC model: II. Parameterization and validation. Journal of Computational Chemistry. 2002;23. pmid:12395429
- 49. Wang J, Wolf RM, Caldwell JW, Kollman PA, Case DA. Development and testing of a general Amber force field. Journal of Computational Chemistry. 2004;25. pmid:15116359
- 50. Jorgensen WL, Chandrasekhar J, Madura JD, Impey RW, Klein ML. Comparison of simple potential functions for simulating liquid water. The Journal of Chemical Physics. 1983;79.
- 51. Krieger E, Vriend G. New ways to boost molecular dynamics simulations. Journal of Computational Chemistry. 2015;36. pmid:25824339
- 52. Krieger E, Nielsen JE, Spronk CAEM, Vriend G. Fast empirical pKa prediction by Ewald summation. Journal of Molecular Graphics and Modelling. 2006;25. pmid:16644253
- 53. Hess B, Bekker H, Berendsen HJC, Fraaije JGEM. LINCS: A Linear Constraint Solver for molecular simulations. Journal of Computational Chemistry. 1997;18.
- 54. Miyamoto S, Kollman PA. Settle: An analytical version of the SHAKE and RATTLE algorithm for rigid water models. Journal of Computational Chemistry. 1992;13.
- 55. Essmann U, Perera L, Berkowitz ML, Darden T, Lee H, Pedersen LG. A smooth particle mesh Ewald method. The Journal of Chemical Physics. 1995;103.
- 56. Berendsen HJC, Postma JPM, Van Gunsteren WF, Dinola A, Haak JR. Molecular dynamics with coupling to an external bath. The Journal of Chemical Physics. 1984;81.
- 57. Krieger E, Koraimann G, Vriend G. Increasing the precision of comparative models with YASARA NOVA—A self-parameterizing force field. Proteins: Structure, Function and Genetics. 2002;47. pmid:11948792
- 58. Mitra S, Dash R. Structural dynamics and quantum mechanical aspects of shikonin derivatives as CREBBP bromodomain inhibitors. Journal of Molecular Graphics and Modelling. 2018;83. pmid:29758466
- 59. Srinivasan E, Rajasekaran R. Computational investigation of curcumin, a natural polyphenol that inhibits the destabilization and the aggregation of human SOD1 mutant (Ala4Val). RSC Advances. 2016;6.
- 60. Yang L, Xiong H, Li X, Li Y, Zhou H, Lin X, et al. Network Pharmacology and Comparative Transcriptome Reveals Biotargets and Mechanisms of Curcumol Treating Lung Adenocarcinoma Patients With COVID-19. Front Nutr. 2022;9: 870370. pmid:35520289
- 61. Gu H, Yuan G. Identification of key genes in SARS-CoV-2 patients on bioinformatics analysis. Bioinformatics; 2020 Aug.
- 62. Hasan MI, Rahman MH, Islam MB, Islam MZ, Hossain MA, Moni MA. Systems Biology and Bioinformatics approach to Identify blood based signatures molecules and drug targets of patient with COVID-19. Informatics in Medicine Unlocked. 2022;28: 100840. pmid:34981034
- 63. Gu H, Yuan G. Identification of potential biomarkers and inhibitors for SARS-CoV-2 infection. medRxiv. 2020.
- 64. Huffman J, Butler-Laporte G, Khan A, Drivas TG, Peloso GM, Nakanishi T, et al. Alternative splicing of OAS1 alters the risk for severe COVID-19. medRxiv. 2021. pmid:33791713
- 65. Zhou S, Butler-Laporte G, Nakanishi T, Morrison DR, Afilalo J, Afilalo M, et al. A Neanderthal OAS1 isoform protects individuals of European ancestry against COVID-19 susceptibility and severity. Nature medicine. 2021;27: 659–667. pmid:33633408
- 66. Magusali N, Graham AC, Piers TM, Panichnantakul P, Yaman U, Shoai M, et al. A genetic link between risk for Alzheimer’s disease and severe COVID-19 outcomes via the OAS1 gene. Brain. 2021;144: 3727–3741. pmid:34619763
- 67. Schoggins J. Defective Viral Rna Sensing Linked to Severe Covid-19. Science. 2021;374: 535–536. pmid:34709914
- 68. Salih D. Genetic variability associated with oligoadenylate synthetase 1, OAS1, in myeloid cells increases the risk of Alzheimer’s disease and severe COVID-19. Brain and Neuroscience Advances. 2021; 30–31.
- 69. Chetta M, Rosati A, Marzullo L, Tarsitano M, Bukvic N. A SARS-CoV-2 host infection model network based on genomic human Transcription Factors (TFs) depletion. Heliyon. 2020;6: e05010. pmid:32984567
- 70. Uddin M, Loney T, Nowotny N, Alsuwaidi H, Varghese R, Deesi Z, et al. Host transcriptomic profiling of COVID-19 patients with mild, moderate, and severe clinical outcomes. 2020. pmid:33425248
- 71. Lee JS, Park S, Jeong HW, Ahn JY, Choi SJ, Lee H, et al. Immunophenotyping of COVID-19 and influenza highlights the role of type I interferons in development of severe COVID-19. Sci Immunol. 2020;5: eabd1554. pmid:32651212
- 72. Huang J, Zhou X, Gong Y, Chen J, Yang Y, Liu K. Network pharmacology and molecular docking analysis reveals the mechanism of asiaticoside on COVID-19. Annals of Translational Medicine. 2022;10. pmid:35280425
- 73. Gong B, Huang L, He Y, Xie W, Yin Y, Shi Y, et al. A genetic variant in IL-6 lowering its expression is protective for critical patients with COVID-19. Sig Transduct Target Ther. 2022;7: 112. pmid:35368020
- 74. Junior GSM, Kurizky PS, Cerqueira SRPS, Barroso DH, Schulte HL, de Albuquerque CP, et al. Enhanced IL-6 and IL-12B Gene Expression After SARS-CoV-2 Infection in Leprosy Patients May Increase the Risk of Neural Damage. The American Journal of Tropical Medicine and Hygiene. 2021;104: 2190. pmid:33819170
- 75. Santa Cruz A, Mendes-Frias A, Oliveira AI, Dias L, Matos AR, Carvalho A. Interleukin-6 is a biomarker for the development of fatal severe acute respiratory syndrome coronavirus 2 pneumonia. Front Immunol. 2021; 12: 613422. 2021. pmid:33679753
- 76. Ascierto PA, Fu B, Wei H. IL-6 modulation for COVID-19: the right patients at the right time? Journal for immunotherapy of cancer. 2021;9. pmid:33837054
- 77. Luo W, Ding R, Guo X, Zhan T, Tang T, Fan R, et al. Clinical data mining reveals Gancao-Banxia as a potential herbal pair against moderate COVID-19 by dual binding to IL-6/STAT3. Computers in biology and medicine. 2022;145: 105457. pmid:35366469
- 78. Bovijn J, Lindgren CM, Holmes MV. Genetic IL-6R variants and therapeutic inhibition of IL-6 receptor signalling in COVID-19–Authors’ reply. The Lancet Rheumatology. 2021;3: e97–e98. pmid:33521676
- 79. Niu W, Wu F, Cao W, Wu Z, Chao Y-C, Peng F, et al. Network pharmacology for the identification of phytochemicals in traditional Chinese medicine for COVID-19 that may regulate interleukin-6. Bioscience Reports. 2021;41. pmid:33146673
- 80. Kumar S. COVID-19: A drug repurposing and biomarker identification by using comprehensive gene-disease associations through protein-protein interaction network analysis. 2020.
- 81. Mondeali M, Bemani P, Hosseini P, Kesheh MM, Bahavar A. Comparative Transcriptomic Analysis Be-tween SARS-COV-2, RSV And Influenza H3N2 Patients Highlights The Use Of IL-6 Inhibitors. J Bioinfo Comp Genom. 2022;5: 1–15.
- 82. Giamarellos-Bourboulis EJ, Netea MG, Rovina N, Akinosoglou K, Antoniadou A, Antonakos N, et al. Complex immune dysregulation in COVID-19 patients with severe respiratory failure. Cell host & microbe. 2020;27: 992–1000. pmid:32320677
- 83. Huang J, Wang Y, Zha Y, Zeng X, Li W, Zhou M. Transcriptome Analysis Reveals Hub Genes Regulating Autophagy in Patients With Severe COVID-19. Frontiers in Genetics. 2022;13. pmid:35923698
- 84. Shen Y-A, Jung J, Shimberg GD, Hsu F-C, Rahmanto YS, Gaillard SL, et al. Development of small molecule inhibitors targeting PBX1 transcription signaling as a novel cancer therapeutic strategy. Iscience. 2021;24: 103297. pmid:34816098
- 85. Fang C, Mei J, Tian H, Liou Y-L, Rong D, Zhang W, et al. CSF3 is a potential drug target for the treatment of COVID-19. Frontiers in Physiology. 2021;11: 605792. pmid:33551833
- 86. Zhou Y, Hou Y, Shen J, Huang Y, Martin W, Cheng F. Network-based drug repurposing for novel coronavirus 2019-nCoV/SARS-CoV-2. Cell discovery. 2020;6: 1–18.
- 87. Laha S, Saha C, Dutta S, Basu M, Chatterjee R, Ghosh S, et al. In silico analysis of altered expression of long non-coding RNA in SARS-CoV-2 infected cells and their possible regulation by STAT1, STAT3 and interferon regulatory factors. Heliyon. 2021;7: e06395. pmid:33688586
- 88. Selvaraj G, Kaliamurthi S, Peslherbe GH, Wei D-Q. Identifying potential drug targets and candidate drugs for COVID-19: biological networks and structural modeling approaches. F1000Research. 2021;10. pmid:33968364
- 89. Yang C, Dou R, Yin T, Ding J. MiRNA-106b-5p in human cancers: diverse functions and promising biomarker. Biomedicine & Pharmacotherapy. 2020;127: 110211. pmid:32422566
- 90. Khan M, Sany M, Us R, Islam M, Islam ABMM. Epigenetic regulator miRNA pattern differences among SARS-CoV, SARS-CoV-2, and SARS-CoV-2 world-wide isolates delineated the mystery behind the epic pathogenicity and distinct clinical characteristics of pandemic COVID-19. Frontiers in genetics. 2020; 765.
- 91. Liang L, Zhao L, Zan Y, Zhu Q, Ren J, Zhao X. MiR-93-5p enhances growth and angiogenesis capacity of HUVECs by down-regulating EPLIN. Oncotarget. 2017;8: 107033. pmid:29291009
- 92. Khokhar M, Tomo S, Purohit P. MicroRNAs based regulation of cytokine regulating immune expressed genes and their transcription factors in COVID-19. Meta gene. 2022;31: 100990. pmid:34722158
- 93. Sardar R, Satish D, Gupta D. Identification of novel SARS-CoV-2 drug targets by host microRNAs and transcription factors co-regulatory interaction network analysis. Frontiers in Genetics. 2020;11: 1105. pmid:33173539
- 94. Angriman F, Ferreyro BL, Burry L, Fan E, Ferguson ND, Husain S, et al. Interleukin-6 receptor blockade in patients with COVID-19: placing clinical trials into context. The Lancet Respiratory Medicine. 2021;9: 655–664. pmid:33930329
- 95. Netherton CL, Wileman T. Virus factories, double membrane vesicles and viroplasm generated in animal cells. Current opinion in virology. 2011;1: 381–387. pmid:22440839
- 96. Sabaka P, Koščálová A, Straka I, Hodosy J, Lipták R, Kmotorková B, et al. Role of interleukin 6 as a predictive factor for a severe course of Covid-19: retrospective data analysis of patients from a long-term care facility during Covid-19 outbreak. BMC infectious diseases. 2021;21: 1–8.
- 97. Murugan NA, Kumar S, Jeyakanthan J, Srivastava V. Searching for target-specific and multi-targeting organics for Covid-19 in the Drugbank database with a double scoring approach. Scientific reports. 2020;10: 1–16.
- 98. de Oliveira OV, Rocha GB, Paluch AS, Costa LT. Repurposing approved drugs as inhibitors of SARS-CoV-2 S-protein from molecular modeling and virtual screening. Journal of Biomolecular Structure and Dynamics. 2021;39: 3924–3933.
- 99. Cagno V, Magliocco G, Tapparel C, Daali Y. The tyrosine kinase inhibitor nilotinib inhibits SARS-CoV-2 in vitro. Basic & clinical pharmacology & toxicology. 2021;128: 621–624. pmid:33232578
- 100. Bouchlarhem A, Haddar L, Lamzouri O, Nasri S, Aichouni N, Bkiyar H, et al. Multiple cranial nerve palsies revealing blast crisis in patient with chronic myeloid leukemia in the accelerated phase under nilotinib during severe infection with SARS-COV-19 virus: case report and review of literature. Radiology Case Reports. 2021;16: 3602–3609. pmid:34422148
- 101. Banerjee S, Yadav S, Banerjee S, Fakayode SO, Parvathareddy J, Reichard W, et al. Drug repurposing to identify nilotinib as a potential SARS-CoV-2 main protease inhibitor: insights from a computational and in vitro study. Journal of chemical information and modeling. 2021;61: 5469–5483. pmid:34666487
- 102. Heidari A, Caissutti A, Henderson M, Schmitt K, Besana E, Esposito J, et al. Recent New Results and Achievements of California South University (CSU) BioSpectroscopy Core Research Laboratory for COVID-19 or 2019-nCoV Treatment: Diagnosis and Treatment Methodologies of “Coronavirus. Journal of Current Viruses and Treatment Methodologies. 2020;1: 3–41.
- 103. Ruan Z, Liu C, Guo Y, He Z, Huang X, Jia X, et al. SARS-CoV-2 and SARS-CoV: Virtual screening of potential inhibitors targeting RNA-dependent RNA polymerase activity (NSP12). Journal of medical virology. 2021;93: 389–400. pmid:32579254
- 104. Chandel V, Sharma PP, Raj S, Choudhari R, Rathi B, Kumar D. Structure-based drug repurposing for targeting Nsp9 replicase and spike proteins of severe acute respiratory syndrome coronavirus 2. Journal of Biomolecular Structure and Dynamics. 2022;40: 249–262. pmid:32838660
- 105. Li Y, Zhang J, Wang N, Li H, Shi Y, Guo G, et al. Therapeutic drugs targeting 2019-nCoV main protease by high-throughput screening. BioRxiv. 2020.
- 106. Encinar JA, Menendez JA. Potential drugs targeting early innate immune evasion of SARS-coronavirus 2 via 2’-O-methylation of viral RNA. Viruses. 2020;12: 525. pmid:32397643
- 107. Sahoo BM, Ravi Kumar BVV, Sruti J, Mahapatra MK, Banik BK, Borah P. Drug repurposing strategy (DRS): Emerging approach to identify potential therapeutics for treatment of novel coronavirus infection. Frontiers in Molecular Biosciences. 2021;8: 628144. pmid:33718434
- 108. Ruan Z, Liu C, Guo Y, He Z, Huang X, Jia X, et al. Potential inhibitors targeting RNA-dependent RNA polymerase activity (NSP12) of SARS-CoV-2. 2020.
- 109. Zhou Y-W, Xie Y, Tang L-S, Pu D, Zhu Y-J, Liu J-Y, et al. Therapeutic targets and interventional strategies in COVID-19: mechanisms and clinical studies. Signal transduction and targeted therapy. 2021;6: 1–25.
- 110. Cho J, Lee YJ, Kim JH, Kim SS, Choi B-S, Choi J-H. Antiviral activity of digoxin and ouabain against SARS-CoV-2 infection and its implication for COVID-19. Scientific reports. 2020;10: 1–8.
- 111. Xing Y, Yin L, Guo M, Shi H, Qi T, Wang L, et al. Therapeutic Monitoring of Plasma Digoxin for COVID-19 Patients Using a Simple UPLC-MS/MS Method. Current Pharmaceutical Analysis. 2021;17: 1308–1316.
- 112. Peltzer B, Lerman BB, Goyal P, Cheung JW. Role for digoxin in patients hospitalized with COVID-19 and atrial arrhythmias. Journal of Cardiovascular Electrophysiology. 2021;32: 880. pmid:33522631
- 113. Rattanawong P, Shen W, El Masry H, Sorajja D, Srivathsan K, Valverde A, et al. Guidance on short-term management of atrial fibrillation in coronavirus disease 2019. Journal of the American Heart Association. 2020;9: e017529. pmid:32515253
- 114. Talluri S. Molecular docking and virtual screening based prediction of drugs for COVID-19. Combinatorial Chemistry & High Throughput Screening. 2021;24: 716–728. pmid:32798373
- 115. Sekhar T. Virtual Screening based prediction of potential drugs for COVID-19. Combinatorial Chemistry & High Throughput Screening. 2020;23.
- 116. Aishwarya S, Gunasekaran K, Margret AA. Computational gene expression profiling in the exploration of biomarkers, non-coding functional RNAs and drug perturbagens for COVID-19. Journal of Biomolecular Structure and Dynamics. 2020; 1–16. pmid:33228475
- 117. Xu J, Xue Y, Zhou R, Shi P, Li H, Zhou J. Drug repurposing approach to combating coronavirus: Potential drugs and drug targets. Med Res Rev. 2021;41: 1375–1426. pmid:33277927
- 118. Jeon S, Ko M, Lee J, Choi I, Byun SY, Park S, et al. Identification of Antiviral Drug Candidates against SARS-CoV-2 from FDA-Approved Drugs. Antimicrob Agents Chemother. 2020;64: e00819–20. pmid:32366720
- 119. Mosharaf MdP Kibria MdK, Hossen MdB Islam MdA, Reza MdS Mahumud RA, et al. Meta-Data Analysis to Explore the Hub of the Hub-Genes That Influence SARS-CoV-2 Infections Highlighting Their Pathogenetic Processes and Drugs Repurposing. Vaccines. 2022;10: 1248. pmid:36016137
- 120. Feng Z, Chen M, Liang T, Shen M, Chen H, Xie X-Q. Virus-CKB: an integrated bioinformatics platform and analysis resource for COVID-19 research. Briefings in Bioinformatics. 2021;22: 882–895. pmid:32715315
- 121. Feng Z, Chen M, Xue Y, Liang T, Chen H, Zhou Y, et al. MCCS: a novel recognition pattern-based method for fast track discovery of anti-SARS-CoV-2 drugs. Briefings in Bioinformatics. 2021;22: 946–962. pmid:33078827
- 122. Ruan Z, Liu C, Guo Y, He Z, Huang X, Jia X, et al. SARS‐CoV‐2 and SARS‐CoV: Virtual screening of potential inhibitors targeting RNA‐dependent RNA polymerase activity (NSP12). J Med Virol. 2021;93: 389–400. pmid:32579254
- 123. Jamalipour Soufi G, Iravani S. Potential inhibitors of SARS-CoV-2: recent advances. Journal of Drug Targeting. 2021;29: 349–364. pmid:33210953
- 124. Lo HS, Hui KPY, Lai H-M, He X, Khan KS, Kaur S, et al. Simeprevir potently suppresses SARS-CoV-2 replication and synergizes with remdesivir. ACS central science. 2021;7: 792–802. pmid:34075346
- 125. J A, Francis D, C.S S, K.G A, C S, Variyar EJ. Repurposing simeprevir, calpain inhibitor IV and a cathepsin F inhibitor against SARS-CoV-2 and insights into their interactions with M pro. Journal of Biomolecular Structure and Dynamics. 2022;40: 325–336. pmid:32873185
- 126. Behera S, Mahapatra N, Tripathy C, Pati S. Drug repurposing for identification of potential inhibitors against SARS-CoV-2 spike receptor-binding domain: An in silico approach. Indian J Med Res. 2021;153: 132. pmid:33818470
- 127. Kadioglu O, Saeed M, Greten HJ, Efferth T. Identification of novel compounds against three targets of SARS CoV-2 coronavirus by combined virtual screening and supervised machine learning. Computers in Biology and Medicine. 2021;133: 104359. pmid:33845270
- 128. Khan RJ, Jha RK, Singh E, Jain M, Amera GM, Singh RP, et al. Identification of promising antiviral drug candidates against non-structural protein 15 (NSP15) from SARS-CoV-2: an in silico assisted drug-repurposing study. Journal of Biomolecular Structure and Dynamics. 2022;40: 438–448. pmid:32885740
- 129. Cheng F-J, Huynh T-K, Yang C-S, Hu D-W, Shen Y-C, Tu C-Y, et al. Hesperidin Is a Potential Inhibitor against SARS-CoV-2 Infection. Nutrients. 2021;13: 2800. pmid:34444960
- 130. Bellavite P, Donzelli A. Hesperidin and SARS-CoV-2: New light on the healthy function of citrus fruits. Antioxidants. 2020;9: 742. pmid:32823497
- 131. Das S, Sarmah S, Lyndem S, Singha Roy A. An investigation into the identification of potential inhibitors of SARS-CoV-2 main protease using molecular docking study. Journal of Biomolecular Structure and Dynamics. 2020; 1–11. pmid:32362245
- 132. Wu C, Liu Y, Yang Y, Zhang P, Zhong W, Wang Y, et al. Analysis of therapeutic targets for SARS-CoV-2 and discovery of potential drugs by computational methods. Acta Pharmaceutica Sinica B. 2020;10: 766–788. pmid:32292689
- 133. Balmeh N, Mahmoudi S, Mohammadi N, Karabedianhajiabadi A. Predicted therapeutic targets for COVID-19 disease by inhibiting SARS-CoV-2 and its related receptors. Informatics in Medicine Unlocked. 2020;20: 100407. pmid:32835083
- 134. Matondo A, Kilembe JT, Ngoyi EM, Kabengele CN, Kasiama GN, Lengbiye EM, et al. Oleanolic Acid, Ursolic Acid and Apigenin from Ocimum basilicum as Potential Inhibitors of the SARS-CoV-2 Main Protease: A Molecular Docking Study. IJPR. 2021; 1–16.
- 135. Kumar A, Choudhir G, Shukla SK, Sharma M, Tyagi P, Bhushan A, et al. Identification of phytochemical inhibitors against main protease of COVID-19 using molecular modeling approaches. Journal of Biomolecular Structure and Dynamics. 2021;39: 3760–3770. pmid:32448034
- 136. Fitriani IN, Utami W, Zikri AT, Santoso P. In Silico Approach of Potential Phytochemical Inhibitor from Moringa oleifera, Cocos nucifera, Allium cepa, Psidium guajava, and Eucalyptus globulus for the treatment of COVID-19 by Molecular Docking. In Review; 2020 Jul.
- 137. Chen H, Zhang Z, Wang L, Huang Z, Gong F, Li X, et al. First clinical study using HCV protease inhibitor danoprevir to treat COVID-19 patients. Medicine (Baltimore). 2020;99: e23357. pmid:33235105
- 138. Zhang Z, Wang S, Tu X, Peng X, Huang Y, Wang L, et al. A comparative study on the time to achieve negative nucleic acid testing and hospital stays between danoprevir and lopinavir/ritonavir in the treatment of patients with COVID‐19. J Med Virol. 2020;92: 2631–2636. pmid:32501538
- 139. Liu J, Zhai Y, Liang L, Zhu D, Zhao Q, Qiu Y. Molecular modeling evaluation of the binding effect of five protease inhibitors to COVID-19 main protease. Chem Phys. 2021;542: 111080. pmid:33519023
- 140. Jonny , Violetta L, Kartasasmita AS, Amirullah Roesli RM, Rita C. Pharmacological Treatment Options for Coronavirus Disease-19 in Renal Patients. Uribarri J, editor. International Journal of Nephrology. 2021;2021: 1–9. pmid:34858665
- 141. Santos-Filho OA. Identification of Potential Inhibitors of Severe Acute Respiratory Syndrome-Related Coronavirus 2 (SARS-CoV-2) Main Protease from Non-Natural and Natural Sources: A Molecular Docking Study. SciELO journals; 2021. p. 1428671 Bytes.
- 142. Teoh SL, Lim YH, Lai NM, Lee SWH. Directly Acting Antivirals for COVID-19: Where Do We Stand? Front Microbiol. 2020;11: 1857. pmid:32849448
- 143. Lotfi M, Hamblin MR, Rezaei N. COVID-19: Transmission, prevention, and potential therapeutic opportunities. Clinica Chimica Acta. 2020;508: 254–266. pmid:32474009
- 144. Beck BR, Shin B, Choi Y, Park S, Kang K. Predicting commercially available antiviral drugs that may act on the novel coronavirus (SARS-CoV-2) through a drug-target interaction deep learning model. Computational and Structural Biotechnology Journal. 2020;18: 784–790. pmid:32280433
- 145. Lovering AL, Seung SL, Kim YW, Withers SG, Strynadka NCJ. Mechanistic and structural analysis of a family 31 α-glycosidase and its glycosyl-enzyme intermediate. Journal of Biological Chemistry. 2005;280. pmid:15501829
- 146. Blatt JM, Weisskopf VF, Critchfield CL. Theoretical Nuclear Physics. American Journal of Physics. 1953;21.