Figures
In Fig 5, the bent arrow, which indicates the transcription start point, and ‘-272’ are positioned incorrectly in the sequence. The electrophoretogram of the 5′ RACE-PCR products of hetR transcripts in the wild type and the mutant is missing. Please see the correct Fig 5 here.
(A) Photomicrographs of Anabaena 7120 and the PhetR-DIF1- strain at 24 h and 48 h after nitrogen stepdown. Frequencies of heterocysts/proheterocysts are indicated. (B) A stretch of sequence upstream of hetR, including the DIF1 motif, potential HetR-binding sequence and the tsp at -272 (left), and the electrophoretogram of the 5′ RACE-PCR products of hetR transcripts in the two strains at 18 h after nitrogen stepdown (right). Each band of the RACE-PCR (indicated with tsp) was confirmed by sequencing.
Accordingly, the caption for Fig 5 has been updated to include updated information. Please see the complete, correct Fig 5 caption here.
In the Discussion, there is an error in the first two sentences of the third paragraph. The correct sentences are:
First, we showed that the DIF1-motif promoter is responsible for the upregulation of hetR in (pro)heterocysts. Substitutions at the DIF1 motif greatly reduced the transcription activity of PhetR; a mutant of Anabaena 7120 with the DIF1 motif of hetR substituted in the genome showed no transcription from the tsp -272 (previously -271, the heterocyst-specific tsp in the wild type [47]).
Reference
- 1. Du Y, Zhang H, Wang H, Wang S, Lei Q, Li C, et al. (2020) Expression from DIF1-motif promoters of hetR and patS is dependent on HetZ and modulated by PatU3 during heterocyst differentiation. PLoS ONE 15(7): e0232383. https://doi.org/10.1371/journal.pone.0232383 pmid:32701963
Citation: Du Y, Zhang H, Wang H, Wang S, Lei Q, Li C, et al. (2022) Correction: Expression from DIF1-motif promoters of hetR and patS is dependent on HetZ and modulated by PatU3 during heterocyst differentiation. PLoS ONE 17(5): e0269050. https://doi.org/10.1371/journal.pone.0269050
Published: May 23, 2022
Copyright: © 2022 Du et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.