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Expression of Concern

Expression of Concern: SH2 Modified STAT1 Induces HLA-I Expression and Improves IFN-γ Signaling in IFN-α Resistant HCV Replicon Cells

  • The PLOS ONE Editors

After this article [1] was published, concerns were raised about western blots in Figs 2, 5 and 7, and microscopy images in Figs 1C, 6 and 8.

Specifically:

  • In Fig 1C in this article, the GR17-1 IFN-γ (-) panel appears similar to the 15–3 Cells IFN-α (+) panel in Fig 7a in [2], and the GR17-1 IFN-γ (+) panel appears similar to the IRF9 panel in Fig 7A in [2].
  • In Fig 2 in this article, the backgrounds of lanes one and two in the p-Jak2 blot appear similar to each other.
  • In Fig 5 in this article, lanes three, four, six, and seven in the GR17-1 blot appear similar to each other.
  • In Fig 6A in this article, the differential interference contrast image for IFN-γ (-) appears similar to the S9-13 IFN-γ (-) STAT1-CC-GFP panel in Fig 6B with 90° rotation. The differential interference contrast image in Fig 6A for IFN-γ (+) appears similar to the S9-13 IFN-γ (+) STAT1-GFP panel in Fig 6B with 90° rotation.
  • In Fig 6B in this article, the GR17-1 IFN-γ (+) STAT1-GFP panel appears similar to the GR17-1 IFN-γ (-) STAT1-CC-GFP panel.
  • In Fig 7 in this article, the GADPH blot appears similar to the HCV blot with an additional band in the final lane for the GADPH blot.
  • In Fig 8 in this article, the S-Huh7 panel appears similar to the Huh-7 Cells IFN-α (-) panel in Fig 7A in [2] and to the Huh-7 Cells IFN-α (+) panel in Fig 7A in [2].

Follow-up on these issues is ongoing; in the meantime, the PLOS ONE Editors issue this Expression of Concern.

References

  1. 1. Poat B, Hazari S, Chandra PK, Gunduz F, Balart LA, Alvarez X, et al. (2010) SH2 Modified STAT1 Induces HLA-I Expression and Improves IFN-γ Signaling in IFN-α Resistant HCV Replicon Cells. PLoS ONE 5(9): e13117. pmid:20949125
  2. 2. Poat B., Hazari S., Chandra P.K. et al. Intracellular expression of IRF9 Stat fusion protein overcomes the defective Jak-Stat signaling and inhibits HCV RNA replication. Virol J 7, 265 (2010). pmid:20939906

Citation: The PLOS ONE Editors (2022) Expression of Concern: SH2 Modified STAT1 Induces HLA-I Expression and Improves IFN-γ Signaling in IFN-α Resistant HCV Replicon Cells. PLoS ONE 17(3): e0266497. https://doi.org/10.1371/journal.pone.0266497

Published: March 31, 2022

Copyright: © 2022 The PLOS ONE Editors. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.