https://orcid.org/0000-0001-7867-7706
Figures
Abstract
Pneumococcal conjugate vaccines reduce the burden of invasive pneumococcal disease, but the sustained effect of these vaccines can be diminished by an increase in disease caused by non-vaccine serotypes. To describe pneumococcal serotype epidemiology in Botswana following introduction of 13-valent pneumococcal conjugate vaccine (PCV-13) in July 2012, we performed molecular serotyping of 268 pneumococcal strains isolated from 221 children between 2012 and 2017. The median (interquartile range) age of the children included in this analysis was 6 (3,12) months. Fifty-nine percent of the children had received at least one dose of PCV-13 and 35% were fully vaccinated with PCV-13. While colonization by vaccine serotypes steadily declined following PCV-13 introduction, 25% of strains isolated more than 3 years after vaccine introduction were PCV-13 serotypes. We also observed an increase in colonization by non-vaccine serotypes 21 and 23B, which have been associated with invasive pneumococcal disease and antibiotic resistance in other settings.
Citation: Patel SM, Shaik-Dasthagirisaheb YB, Congdon M, Young RR, Patel MZ, Mazhani T, et al. (2022) Evolution of pneumococcal serotype epidemiology in Botswana following introduction of 13-valent pneumococcal conjugate vaccine. PLoS ONE 17(1): e0262225. https://doi.org/10.1371/journal.pone.0262225
Editor: Jose Melo-Cristino, Universidade de Lisboa Faculdade de Medicina, PORTUGAL
Received: June 27, 2021; Accepted: December 20, 2021; Published: January 5, 2022
Copyright: © 2022 Patel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All data files, code, and output are available on GitHub (URL: https://github.com/swetamp/PCVSero).
Funding: This research was supported by a Merck Investigator Studies Program Grant (MISP #59310 to M.S.K; URL: https://engagezone.msd.com/misp.php). S.M.P. was supported by a VECD Global Health Fellowship, funded by the Office of AIDS Research (OAR) and the Fogarty International Center (FIC) of the National Institutes of Health (NIH; D43 TW009337; URL: https://www.vumc.org/vecd/home). M.S.K. was supported by an Early Career Award from the Thrasher Research Fund (URL: https://www.thrasherresearch.org/early-career-award?lang=eng), a Burroughs Wellcome/American Society of Tropical Medicine and Hygiene Postdoctoral Fellowship in Tropical Infectious Diseases (URL: https://www.astmh.org/awards-fellowships-medals/awards-and-fellowships/burroughs-wellcome-fund-astmh-postdoctoral-fellows), an NIH Career Development Award (K23-AI135090; URL: https://www.niaid.nih.gov/grants-contracts/mentored-patient-oriented-research-career-development-award-k23), and a CIPHER grant from the International AIDS Society, supported by ViiV Healthcare (URL: https://www.iasociety.org/HIV-Programmes/Programmes/Paediatrics-CIPHER/CIPHER-Grant-Programme). The views expressed in this publication do not necessarily reflect the official policies of the International AIDS Society or ViiV Healthcare. M.S.K. and C.K.C. received financial support from the NIH through the Duke Center for AIDS Research (P30-AI064518; URL: https://cfar.duke.edu/). A.P.S and K.A.F received support from the Children’s Hospital of Philadelphia and A.P.S. and T.A-M. through core services from the Penn Center for AIDS Research, an NIH-funded program (P30-AI045008; URL https://www.med.upenn.edu/cfar/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
Streptococcus pneumoniae is a leading cause of child mortality worldwide. While pneumococcal conjugate vaccines effectively prevent invasive pneumococcal disease (IPD) caused by vaccine serotypes, their use has been accompanied by an increase in disease caused by non-vaccine serotypes [1]. Changes in nasopharyngeal carriage of pneumococcal serotypes often parallel changes in serotypes that cause IPD [2]. Carriage studies can thus provide valuable information on the impact of pneumococcal vaccines in countries lacking robust IPD surveillance. We sought to describe temporal trends in pneumococcal serotype carriage among children in Botswana following introduction of 13-valent pneumococcal conjugate vaccine (PCV-13).
Methods
Study setting and design
Botswana introduced PCV-13 (Prevnar-13; Pfizer) into its immunization program in July 2012. The vaccine is administered at 2, 3, and 4 months of age and was introduced without a catch-up campaign. The estimated national coverage rate for the complete vaccine series in infants ranged from 52% after PCV-13 introduction in 2012 to 89% in 2017 [3]. This research used nasopharyngeal swab samples collected from children under 24 months of age enrolled in two prospective cohort studies in Botswana between April 2012 and December 2017. Samples collected between 2012 and 2016 were obtained from children enrolled in a prospective cohort study of infants and young children with and without pneumonia, the design of which was previously described in detail [4]. Briefly, children 1–23 months of age who either presented to a tertiary hospital in Gaborone with symptoms meeting the World Health Organization (WHO) definition of pneumonia or who presented for routine or acute care at a public clinic in the Gaborone area were eligible for enrollment. Exclusion criteria included the presence of a chronic medical condition predisposing to pneumonia (other than HIV infection), hospitalization in the prior 14 days, and asthma. One nasopharyngeal swab was collected from each child at the time of enrollment. Samples collected between 2016 and 2017 were obtained from infants enrolled in a birth cohort study of mother-infant pairs recruited within 72 hours of delivery in and around Gaborone, Botswana. Exclusion criteria for this study included birth weight <2000 g, birth by Caesarian section, and multiple gestation pregnancy. Infants were seen every 1 to 2 months until they reached 12 months of age, and a nasopharyngeal swab was collected at each visit. In both studies, children of mothers with documented negative HIV testing at or prior to enrollment were classified as HIV-unexposed, uninfected. Children of mothers with HIV were classified as HIV-exposed, uninfected (HEU) if they tested negative for HIV at or after 6 weeks of age if exclusively formula fed, at least 6 weeks after breastfeeding cessation, or at enrollment.
Laboratory methods
Nucleic acid from nasopharyngeal samples was extracted on the NucliSens easyMAG platform and tested for S. pneumoniae using a quantitative PCR assay targeting the autolysin gene (lytA) gene [5]. Samples with >500 copies/mL of lytA were considered to have detectable S. pneumoniae DNA and underwent real-time sequential multiplex PCR as previously described [6] with primers characterized at Centers for Disease Control and Prevention (CDC) that detect 40 common serotypes or serogroups [7]. Real-Time PCR (RT-PCR) reagents (SsoAdvanced Universal Supermixes) were purchased from Bio-Rad Laboratories (Hercules, CA). PCR primers and probes were synthesized by Sigma-Millipore (St. Louis, MO). RT-PCR conditions used were as follows: 95°C for 15 min, followed by 35 amplification cycles of 94°C for 30 sec, 54°C for 90 sec, and 72°C for 60 sec. A final hold was performed at 72°C for 10 min. RT-PCR assays were performed according to manufacturer instructions using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA). Before the multiplex assays, primers were tested using serotype-specific positive and negative controls for specificity. A Ct value ≤35 was considered positive for the reaction. In addition to the Ct value, melting curves of each reaction were carefully evaluated before considering results for the reaction. Cultures were not performed. For children with more than one nasopharyngeal sample collected over time, the earliest sample from which S. pneumoniae was detected was chosen for serotyping.
Statistical analysis
We classified serotypes/serogroups into PCV-13 serotypes (1, 3, 4, 5, 6A, 6B, 7F/A, 9V/A, 14, 18C/F/B/A, 19A, 19F, 23F) and non-vaccine serotypes (2, 6C/D, 11A, 12F, 15A/F, 15B, 16F, 21, 22F, 23A, 23B, 33F, and 35B). Analyses were limited to nasopharyngeal samples with identifiable pneumococcal serotypes by PCR. We compared categorical variables using Chi-square or Fisher’s exact tests and continuous variables using Wilcoxon rank sum tests. We used multinomial logistic regression, adjusted a priori for age and HIV exposure status, to evaluate for an association between collection year and serotype category [8–11]. Statistical analyses were conducted using R version 3.6.2.
Ethical statement
Written informed consent was obtained from parents or legal guardians for all children enrolled in this study. This study was approved by the Health Research and Development Committee (Ministry of Health, Botswana) and institutional review boards at Princess Marina Hospital, the University of Pennsylvania, McMaster University, Boston University, and Duke University.
Results
Patient characteristics
S. pneumoniae was identified in 1,304 of 2,494 (52%) nasopharyngeal samples collected from 550 of 841 (65%) enrolled children. Sufficient DNA extract was available for serotyping samples from 316 children, and we identified 268 S. pneumoniae strains from a final study population of 221 children (Fig 1). Of the 221 children included in this analysis, 124 (56%) children had pneumonia. Thirty-eight (17%) children were concurrently colonized with two or more serotypes. The number of nonserotypeable samples identified by year during the study period is shown in S1 Table. Characteristics of the study population are shown in Table 1. Median (IQR) age of children with PCV-13 serotypes was higher than that of children with non-vaccine serotypes [8 (3, 14) months vs. 4 (2, 10) months; P = 0.001], but we did not observe a difference in serotype classification by HIV status, sex, or number of PCV-13 doses received. Forty-eight percent of strains isolated from fully vaccinated children were PCV-13 serotypes.
Serotype epidemiology over time
The serotypes isolated over the course of the study period are shown in Table 2. The proportion of PCV-13 serotypes declined from 80 of 115 (70%) isolates in 2012–2013 to 22 of 88 (25%) isolates in 2016–2017 (Fig 2). The most common serotypes isolated in 2012–2013 were 6A/6B and 19F. In 2016–2017, the most frequently identified serotypes were 21 and 23B. Four percent of strains between 2012–2015 were classified as serotype 21, compared to 9% of strains in 2016–2017 (P = 0.09). One percent of strains between 2012–2015 were classified as serotype 23B, compared to 28% of strains in 2016–2017 (P < 0.001). The likelihood of a pneumococcal strain being a PCV-13 serotype decreased by 36% per year during the study period (relative risk 0.64; 95% confidence interval: 0.54, 0.76, P<0.001). The detailed results of the multinomial regression analysis are shown in Table 3. Among children enrolled between 2012 and 2016, the proportion of strains that were classified as PCV-13 was similar in children with pneumonia compared to healthy children (60% vs 67%, respectively; P = 0.43).
The bars represent the serotypes of identified pneumococcal isolates collected from children in Botswana by year of enrollment. Vaccine serotypes are shaded red to orange, while non-vaccine serotypes are presented in shades of blue. The number of colonizing isolates serotyped is displayed below the enrollment year.
Discussion
PCV-13 introduction was associated with a substantial reduction in nasopharyngeal carriage of vaccine serotypes among children in Botswana. Despite high national vaccine uptake, however, the proportion of colonizing strains that were PCV-13 serotypes remained high (25%) five years after vaccine introduction. Our findings are consistent with prior studies in children conducted after PCV-13 introduction in South Africa, where vaccine serotypes comprised 22% of colonizing pneumococcal isolates, and Malawi, where vaccine serotypes were isolated from 23% of children [12, 13]. However, our results contrast with prior studies conducted in England and Belgium, where the prevalence of carriage of vaccine serotypes declined to <6% within five years of PCV-13 introduction [14, 15]. These discordant findings could reflect differences in herd immunity due to varied vaccine administration schedules or population prevalence of HIV infection or other immunocompromising conditions. Additionally, the pneumococcal antibody thresholds needed to protect against nasopharyngeal carriage among children in low- and middle-income countries may be higher than the thresholds needed in high-income countries [16]. As PCV-13 serotypes remain responsible for a large proportion of IPD cases in low- and middle-income countries despite increasing use of PCV-13, residual carriage in these settings may blunt the impact of pneumococcal vaccination on childhood mortality [17].
We also observed changes in the prevalence of several pneumococcal serotypes commonly implicated in clinical disease. Early in the study period, serotypes 6A, 6B, and 19F were the most commonly identified serotypes. Strains belonging to serogroups 6 and 19 were among the most common causes of IPD prior to the introduction of conjugate vaccines, and reduced circulation of these serotypes may have contributed to the decline in hospitalizations and deaths due to childhood pneumonia in Botswana following PCV-13 introduction [17, 18]. By comparison, the serotype epidemiology in Botswana following PCV-13 introduction differs from other settings in sub-Saharan Africa. For example, serotype 15B/C became the dominant pneumococcal serotype in South Africa after PCV-13 introduction [12], but the proportion of serotype 15B/C isolates remained relatively stable over time among children in our cohort. During the final two years of our study, the most common serotypes identified were the non-vaccine serotypes 21 and 23B. Though it is unclear if this increase in serotype 23B will be sustained, its rise is particularly concerning given that it has become one of the most common serotypes isolated from children with IPD following PCV-13 introduction in other settings and is often resistant to common antimicrobials [15, 19]. The current contribution of these serotypes to IPD in Botswana is unknown, but their emergence illustrates the need for more effective vaccines to prevent pneumococcal infections during childhood.
This study has a number of limitations. First, the study population was composed of children from two distinct cohorts that differed based on characteristics including age and PCV-13 vaccination status. For example, the association between age and pneumococcal serotype epidemiology may be confounded by time period, because children enrolled between 2012 and 2016 were older than children enrolled between 2016 and 2017. Additionally, the inclusion of children with pneumonia may have biased our results towards the identification of PCV-13 serotypes. However, the proportion of children with pneumonia colonized with PCV-13 serotypes was similar to that of healthy controls enrolled during the same study period. Furthermore, children were recruited from a geographically restricted area of Botswana, which limits the generalizability of our findings. The molecular assays that we used identified only 40 of the over 100 known pneumococcal serotypes. We also did not identify a serotype in approximately one-third of samples that underwent molecular serotyping, which likely reflects the presence of serotypes not identified by the CDC primers, non-typeable strains, or other Streptococcus species containing the lytA gene. While CDC primers encompass all PCV-13 serotypes, many non-vaccine serotypes are not represented in the CDC primer set; consequently, pneumococcal strains that were present in the nonserotypeable samples were more likely to be non-vaccine serotype strains and our decision to exclude these samples from our analysis may have biased our results. Finally, we only had data on colonizing serotypes from young children and were unable to evaluate for changes in serotype epidemiology among the strains that cause IPD or colonize adults.
In summary, following PCV-13 introduction in Botswana, we observed a reduction in nasopharyngeal carriage of vaccine serotypes accompanied by an increase in carriage of non-vaccine serotypes associated with IPD and antibiotic resistance in other settings. While existing vaccines dramatically reduced pneumococcal disease among children worldwide, further gains will depend on strategies to strengthen herd immunity and address the rise of clinically significant non-vaccine serotypes through the development of expanded valency or non-capsule-based pneumococcal vaccines.
Supporting information
S1 Table. Classification of all lytA+ samples.
https://doi.org/10.1371/journal.pone.0262225.s001
(DOCX)
Acknowledgments
The authors would like to thank Copan Italia (Brescia, Italy) for donation of the media and flocked swabs used in the collection of nasopharyngeal specimens in these research studies. The authors offer sincere thanks to the children and families who participated in this study.
References
- 1. Rodenburg GD, de Greeff SC, Jansen AG, de Melker HE, Schouls LM, Hak E, et al. Effects of pneumococcal conjugate vaccine 2 years after its introduction, the Netherlands. Emerg Infect Dis. 2010;16(5):816–23. Epub 2010/04/23. pmid:20409372; PubMed Central PMCID: PMC2953990.
- 2. Weinberger DM, Bruden DT, Grant LR, Lipsitch M, O’Brien KL, Pelton SI, et al. Using Pneumococcal Carriage Data to Monitor Postvaccination Changes in Invasive Disease. American Journal of Epidemiology. 2013;178(9):1488–95. pmid:24013204
- 3.
Botswana: WHO and UNICEF estimates of immunization coverage: 2019 revision. 2019 July 2. Report No.
- 4. Kelly MS, Smieja M, Luinstra K, Wirth KE, Goldfarb DM, Steenhoff AP, et al. Association of respiratory viruses with outcomes of severe childhood pneumonia in Botswana. PLoS One. 2015;10(5):e0126593. pmid:25973924; PubMed Central PMCID: PMC4431806.
- 5. Whelan FJ, Verschoor CP, Stearns JC, Rossi L, Luinstra K, Loeb M, et al. The loss of topography in the microbial communities of the upper respiratory tract in the elderly. Ann Am Thorac Soc. 2014;11(4):513–21. Epub 2014/03/08. pmid:24601676.
- 6. Pimenta FC, Roundtree A, Soysal A, Bakir M, du Plessis M, Wolter N, et al. Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden. J Clin Microbiol. 2013;51(2):647–52. Epub 2012/12/12. pmid:23224094; PubMed Central PMCID: PMC3553924.
- 7.
List of oligonucleotide primers used in 41 conventional multiplex PCR assays for pneumococcal serotype deduction of 70 serotypes. In: Laboratory S, editor.: Centers for Disease Control and Prevention; 2014.
- 8. Bliss SJ , O’Brien KL, Janoff EN, Cotton MF, Musoke P, Coovadia H, et al. The evidence for using conjugate vaccines to protect HIV-infected children against pneumococcal disease. Lancet Infect Dis. 2008;8(1):67–80. Epub 2007/11/03. pmid:17974480.
- 9. Hausdorff WP, Feikin DR, Klugman KP. Epidemiological differences among pneumococcal serotypes. Lancet Infect Dis. 2005;5(2):83–93. Epub 2005/02/01. pmid:15680778.
- 10. Madhi SA, Moreira M, Koen A, van Niekerk N, de Gouveia L, Jose L, et al. Impact of HIV status and vaccination schedule on bacterial nasopharyngeal carriage following infant immunisation with the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine in South Africa. Vaccine. 2020;38(10):2350–60. Epub 2020/02/10. pmid:32035706.
- 11. Swarthout TD, Fronterre C, Lourenco J, Obolski U, Gori A, Bar-Zeev N, et al. High residual carriage of vaccine-serotype Streptococcus pneumoniae after introduction of pneumococcal conjugate vaccine in Malawi. Nat Commun. 2020;11(1):2222. Epub 2020/05/08. pmid:32376860; PubMed Central PMCID: PMC7203201.
- 12. Dube FS, Ramjith J, Gardner-Lubbe S, Nduru P, Robberts FJL, Wolter N, et al. Longitudinal characterization of nasopharyngeal colonization with Streptococcus pneumoniae in a South African birth cohort post 13-valent pneumococcal conjugate vaccine implementation. Sci Rep. 2018;8(1):12497. Epub 2018/08/23. pmid:30131607; PubMed Central PMCID: PMC6104038.
- 13. Heinsbroek E, Tafatatha T, Phiri A, Swarthout TD, Alaerts M, Crampin AC, et al. Pneumococcal carriage in households in Karonga District, Malawi, before and after introduction of 13-valent pneumococcal conjugate vaccination. Vaccine. 2018;36(48):7369–76. Epub 2018/10/26. pmid:30352744; PubMed Central PMCID: PMC6238076.
- 14. van Hoek AJ, Sheppard CL, Andrews NJ, Waight PA, Slack MP, Harrison TG, et al. Pneumococcal carriage in children and adults two years after introduction of the thirteen valent pneumococcal conjugate vaccine in England. Vaccine. 2014;32(34):4349–55. Epub 2014/03/25. pmid:24657717.
- 15. Wouters I, Desmet S, Van Heirstraeten L, Blaizot S, Verhaegen J, Van Damme P, et al. Follow-up of serotype distribution and antimicrobial susceptibility of Streptococcus pneumoniae in child carriage after a PCV13-to-PCV10 vaccine switch in Belgium. Vaccine. 2019;37(8):1080–6. Epub 2019/01/23. pmid:30665775.
- 16. Voysey M, Fanshawe TR, Kelly DF, O’Brien KL, Kandasamy R, Shrestha S, et al. Serotype-Specific Correlates of Protection for Pneumococcal Carriage: An Analysis of Immunity in 19 Countries. Clin Infect Dis. 2018;66(6):913–20. Epub 2017/10/27. pmid:29069415.
- 17. Turner P, Leab P, Ly S, Sao S, Miliya T, Heffelfinger JD, et al. Impact of 13-Valent Pneumococcal Conjugate Vaccine on Colonization and Invasive Disease in Cambodian Children. Clin Infect Dis. 2020;70(8):1580–8. pmid:31175819.
- 18. Congdon M HH, Young RR, Cunningham CK, Enane LA, Arscott-Mills T, Banda FM, et al. Effect of Haemophilus influenzae type b and 13-valent pneumococcal conjugate vaccines on childhood pneumonia hospitalizations and deaths in Botswana. Clin Infect Dis. 2020:In press.
- 19. Kaplan SL, Barson WJ, Lin PL, Romero JR, Bradley JS, Tan TQ, et al. Invasive Pneumococcal Disease in Children’s Hospitals: 2014–2017. Pediatrics. 2019;144(3). Epub 2019/08/20. pmid:31420369.