Following publication of this article [1], concerns were raised about the availability of the underlying data. This notice updates readers about the availability of the data with a corrected Data Availability statement.
The Data Availability statement is as follows:
The sequence and assembly data for each of the 40 gut microbiota samples is deposited at the CNGB Sequence Archive (https://db.cngb.org/cnsa/) under project accession number CNP0001174. The associated individual-level demographic data is deposited under accession number CNP0001169 (restricted data, for access requests visit https://db.cngb.org/data_access/).
Sample accession numbers are provided as Supporting Information on this notice (S1 File). Samples are grouped as follows:
Kazak, normal glucose tolerance (KNGT) = samples 1–10 (sample accessions CNS0254252-CNS0254261)
Kazak, diabetes mellitus type 2 (KDM2) = samples 11–20 (sample accessions CNS0254262—CNS0254271)
Uygur, normal glucose tolerance (UNGT) = samples 21–30 (sample accessions CNS0254272—CNS0254281)
Uygur, diabetes mellitus type 2 (UDM2) = samples 31–40 (sample accessions CNS0254282—CNS0254291)
Previously published studies on the same samples used 16S rDNA real time PCR to assess correlation between genus-level abundance of bacteria with physical and biochemical indicators in the same four groups [2]. Another previous study on the same samples reported physical and biochemical measurements as well as levels of indirect markers of bacterial exposure in the same four groups [3].
The following additional clarifications are provided regarding statistical analyses in [1]:
Sample size calculation
The sample size calculation uses the following experimental data from [2]:
By 16S rDNA real time PCR in genus level, the abundance of Veillonella spp and C. coccoides subgroups in Kazaks was obtained (KNGT VS KDM2: 2.10±0.20 VS 3.05±0.39; KNGT VS KDM2: 4.27±0.32 VS 4.80±0.19 Fig 1 and 2 of [2]). According to the following formula, we calculated the sample sizes of Kazaks as 4.47and 10.35, respectively.
The sample size calculation uses the following formula to compare two sample means:
Where numerator: σ is total variance; denominator: δ is mean difference between two groups. α = 0.05, β = 0.9, Zα = 1.96 and Zβ = 1.28
Considering the previously published prevalence of KDM2 (1.47%) [4] and the difficulties collecting KDM2 samples, the number of feces samples for Illumina sequencing was 10 (shown in table 1 and 2 [1]).
Data in Tables 1, 2 and 4 of [1] are reported as mean ± SD.
References
- 1. Wang Y, Luo X, Mao X, Tao Y, Ran X, Zhao H, et al. (2017) Gut microbiome analysis of type 2 diabetic patients from the Chinese minority ethnic groups the Uygurs and Kazaks. PLoS ONE 12(3): e0172774. https://doi.org/10.1371/journal.pone.0172774 pmid:28328990
- 2. Zhao H, Li L, Mao X, Zhang R, Xiong J, Wang Y. The relationship of Veillonella spp and C. coccoides subgroup levels in gut microbiota with type 2 diabetes mellitus in Uygurs and Kazaks of Xinjiang. Chinese Journal of Microecology. 2015;27(1):6–10.
- 3. Zhang P, Li L, Xiong J, Zhao H, Mao X, Wang Y. The relationship of LBP, sCD14 levels with type 2 diabetes mellitus in Uygurs and Kazaks of Xinjiang. Chinese Journal of Microecology. 2014;26 (2):139–143.
- 4. Tao Y, Mao X, Xie Z, Ran X, Liu X, Wang Y, et al. The Prevalence of Type 2 Diabetes and Hypertension in Uygur and Kazak Populations. Cardiovasc Toxicol. 2008; 8:155–159. pmid:18777166
Citation: Wang Y, Luo X, Mao X, Tao Y, Ran X, Zhao H, et al. (2021) Correction: Gut microbiome analysis of type 2 diabetic patients from the Chinese minority ethnic groups the Uygurs and Kazaks. PLoS ONE 16(3): e0249015. https://doi.org/10.1371/journal.pone.0249015
Published: March 24, 2021
Copyright: © 2021 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.