Figures
Abstract
In last decades, a large body of evidence clarified nitrogen isotope composition (δ15N) patterns in plant leaves, roots and metabolites, showing isotopic fractionation along N uptake and assimilation pathways, in relation to N source and use efficiency, also suggesting 15N depletion in plant DNA. Here we present a manipulative experiment on Brassica napus var. oleracea, where we monitored δ 15N of purified, lyophilized DNA and source leaf and root materials, over a 60-days growth period starting at d 60 after germination, in plants initially supplied with a heavy labelled (δ 15NAir-N2 = 2100 mUr) ammonium nitrate solution covering nutrient requirements for the whole observation period (470 mg N per plant) and controlling for the labelled N species (ṄH4, ṄO3 and both). Dynamics of Isotopic Ratio Mass Spectrometry (IRMS) data for the three treatments showed that: (1) leaf and root δ 15N dynamics strictly depend on the labelled chemical species, with ṄH4, ṄO3 and ṄH4ṄO3 plants initially showing higher, lower and intermediate values, respectively, then converging due to the progressive NH4+ depletion from the nutrient solution; (2) in ṄH4ṄO3, where δ15N was not affected by the labelled chemical species, we did not observe isotopic fractionation associated to inorganic N uptake; (3) δ15N values in roots compared to leaves did not fully support patterns predicted by differences in assimilation rates of NH4+ and NO3-; (4) DNA is depleted in 15N compared to the total N pools of roots and leaves, likely due to enzymatic discrimination during purine biosynthesis. In conclusion, while our experimental setup did not allow to assess the fractionation coefficient (ε) associated to DNA bases biosynthesis, this is the first study specifically reporting on dynamics of specific plant molecular pools such as nucleic acids over a long observation period with a heavy labelling technique.
Citation: Foscari A, Leonarduzzi G, Incerti G (2021) N uptake, assimilation and isotopic fractioning control δ 15N dynamics in plant DNA: A heavy labelling experiment on Brassica napus L. PLoS ONE 16(3): e0247842. https://doi.org/10.1371/journal.pone.0247842
Editor: Matheus C. Carvalho, Southern Cross University, AUSTRALIA
Received: July 10, 2020; Accepted: February 12, 2021; Published: March 11, 2021
Copyright: © 2021 Foscari et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the manuscript and its Supporting information files.
Funding: Financial support for this study was provided to GI by University of Udine (www.uniud.it), Di4A, PRID2017. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
In last decades a large body of evidence clarified the main sources of variation in nitrogen isotope composition (δ 15N) at intra-plant level (review in [1]), showing that isotopic fractionation occurs in relation to the chemical species, content and bioavailability of inorganic and organic N in the substrate, with known biochemical mechanisms [2].
Plant isotope composition firstly relates to δ 15N and relative fractions in the substrate of different N sources, such as NH4+, NO3-, organic N, or N2 in the case of species symbiotic with diazotrophic prokaryotes [3]. Plant δ 15N also varies compared to that of soil N due to different uptake mechanisms, assimilation pathways, and rates of N recycling, which can all discriminate against the heavy isotope [1]. As an example, NO3- uptake is mediated by either constitutive carrier system with high-substrate affinity or non-saturable transport mechanisms with low-substrate affinity, which act at low (0–500 μM) and high (> 500 μM) NO3- concentrations in the substrate, respectively. Both transport systems produce isotopic fractionation [4–6], although plant-to-soil δ 15N variation is larger when NO3- is the primary N source, and smaller when NH4+ is used [1]. On the other hand, species- and cultivar-specific effects can outcompete those of the N chemical species [5, 7], which led to measuring foliar δ 15N to understand the physiological mechanisms underlying N use differences among co-occurring species [1].
Considering discrimination against 15N during inorganic N assimilation [3], several previous studies focused on the enzymatic fractionation by nitrate reductase and glutamine synthetase [8–12] and its effects on intra-plant δ 15N variation. Nitrate is assimilated both in roots and leaves, where the content of assimilation enzymes and the rate of assimilation can affect the resulting δ 15N [13]. However, the NO3- available for assimilation in leaves is enriched relative to root NO3- because it originates from a pool that has already been exposed to fractionation during root assimilation, leading to higher δ 15N of leaves compared to roots [1]. However, it has been recently reported that NO3- can be enriched in 15N in roots compared to leaves, due to nitrate circulation and compartmentalization, in particular by phloematic backflow from the leaves [14]. Differently, NH4+ is immediately assimilated in the root, therefore root vs. leaf δ 15N differences are less affected by NH4+ assimilation, as organic nitrogen in shoots and roots is the product of a single assimilation event [1].
Further contributions to intra-plant δ 15N variation rely on isotopic fractionation during xylematic [15] and re-allocation [16] flows, as well as N depletion by NH3 and NO2 volatilization, although the latter process, being limited to the leaf senescence stage, likely bears negligible effects [12]. Finally, the role of plant symbionts such as mycorrhizae and N-fixing rhizosphere bacteria were investigated in both field and controlled conditions [17], showing interesting dynamics [18] but limited effects, in relation to their negligible mass compared to that of the plant [19].
More recently, isotopic fractionation has been investigated along specific metabolic pathways by IRMS analysis after purification of different leaf metabolites, including amino acids, nucleic acids and chlorophylls [20] or by compound-specific stable isotope analysis (CSIA), where IRMS is coupled with GC-MS or LC-MS interface to separate different metabolites before isotopic analysis [21–23]. Differences of δ 15N among different molecular N pools depend on isotopic discrimination by most enzymes of primary N metabolism [e.g. Glu synthase, transaminases, Asn synthetase, etc., 10]. Accordingly, Gauthier et al. observed by CSIA a different δ 15N in different N molecular pools in Brassica napus leaves, corresponding to a predominant effect of enzymatic discrimination in amino acid metabolic pathways, compared to that associated to the inorganic N source [20]. Moreover, the N pool of leaf DNA, purified by standard methods, lyophilized and isotopically analyzed by EA-IRMS, was isotopically depleted compared to amino acids, consistent to discrimination associated with the synthesis of bases [20]. Several enzymes involved in pyrimidine synthesis discriminate the heavy isotope, such as carbamoyl phosphate synthetase [24], aspartate carbamoyltransferase [25], dihydroorotase [26], orotate phosphoribosyltransferase [27]. In the case of purine synthesis, amino acids such as Glu, Gln, Asp, and Gly are the N sources, but isotope effects along these metabolic ways are not yet fully clarified. Consequently, the δ 15N of leaf DNA is expected to be lower compared to other leaf N pools [20]. Accordingly, lower δ 15N is expected in plant DNA compared to the source material from which it is purified, whose δ 15N value results from the average of different molecular pools, weighted by their relative mass fraction [28]. However, changes of δ 15N in plant materials during plant development could affect the expected pattern, as related to possible decoupling in time of DNA biosynthesis and inorganic N uptake and assimilation dynamics, and hence of their fractionation effects.
Therefore, in this study we set up a manipulative experiment in controlled conditions on Brassica napus var. oleracea, monitoring δ 15N of purified DNA and source leaf and root materials, over a 60-days growth period starting at d 60 after germination, in plants initially supplied with a heavy labelled ammonium nitrate solution and controlling for the labelled N species (either NO3-, NH4+ or both). We assumed that the magnitude of isotope effects is small enough that they generally do not perturb plant growth dynamics when compared to the unlabelled control [29]. Our specific hypotheses and expected outcomes were that: (1) leaf and root δ 15N dynamics strictly depend on the labelled chemical species, as related to a limiting effect of NH4+ concentration on the uptake of NO3- [8]. Accordingly, plants supplied with either labelled NH4+, labelled NO3- or both labelled species (thereafter referred to as ṄH4, ṄO3 and ṄH4ṄO3, respectively) should initially show higher, lower and intermediate values, respectively. Then, the progressive NH4+ depletion from the nutrient solution should correspond to an increase of NO3- uptake rate, with ṄH4 and ṄO3 plant materials showing progressively decreasing and increasing δ 15N, respectively. (2) In ṄH4ṄO3 plants, where δ 15N is not affected by the labelled N chemical species, we tested the occurrence of isotopic fractionation associated to inorganic N uptake [4, 6, 30], expecting an increase of δ 15N over time due to a progressive 15N enrichment in the N pool residual in the pot solution. (3) Differences in assimilation rates in roots compared to leaves should produce, at a given observation stage, higher δ 15N values in ṄH4 roots compared to ṄH4 leaves and in ṄO3 leaves compared to ṄO3 roots, with ṄH4ṄO3 materials showing intermediate values; (4) Consistent to expectations [20], DNA is depleted in 15N compared to the other molecular N pools, and thus to the source plant material, due to enzymatic discrimination during purine biosynthesis.
Materials and methods
Our experimental design is summarized in Fig 1, including seed germination and potting, isotopic labelling, periodic destructive sampling, plant DNA extraction and purification and CHN-IRMS analysis.
Six manipulative steps are shown along the experiment timeline: 1) seeds germination at d 0; 2) potting at d 5; 3) labelling with ammonium nitrate solution, including 3 treatments (T, each on 30 replicated pots) with the same isotopic ratio, i.e. δ15NAir-N2 = 2100 mUr, but differing by the labelled chemical species (ṄH4, ṄO3 or ṄH4ṄO3), plus the untreated control (C, 10 replicates) administered with the same dose of unlabelled ammonium nitrate (N); 4) destructive sampling (6 replicates per treatment at each of 5 observation stages from d 60 to d 120) and separation of leaf, stem and root materials (L, S and R, respectively); 5) DNA extraction and purification from leaf ad root materials; 6) CHN-IRMS analysis of dry aliquots of plant materials and corresponding DNA samples. See methods for further details.
Plant material, sowing and potting
Commercial seeds of Brassica napus var. oleifera, cultivar Gordon (KWS SAAT SE & Co. KGaA, Germany), were imbibed with Milli-RO water for 24 h into in 50 mL lab grade tubes, then transferred to plastic saucers filled with dried, quartz sand substrate (GESTECO Spa, Italy; physical-chemical features in S1 Table) and kept in a growing room in controlled optimal conditions (T = 22/20 °C, photoperiod 12 h, RH = 50%, PAR 600 μmol photons m-2 s-1). Five days after germination (i.e. d 5), seedlings were transplanted in pots (2 seedlings per pot) previously filled with 1.3 kg of substrate. Each pot had draining holes allowing drainage and preventing hypoxia, and was placed on a saucer to prevent nutrient loss. One-hundred pots were considered, corresponding to 30 replicates for each of 3 labelling treatments, plus 10 unlabelled controls.
Labelling and nutrient solution
Three different N labelling treatments with different labelled chemical species (either ammonium, nitrate or both) were considered, all with equal isotopic ratio (δ 15NAir-N2 = 2100 mUr). Such extremely high value was used in order to ensure the detectability of 15N depletion in leaf and root DNA along the observation period, in absence of previous quantitative evidence on the possible enzymatic discrimination coefficient [20]. Ammonium nitrate solutions for labelling were prepared by mixing a water solution of commercial NH4NO3 (Sigma-Aldrich, USA, δ 15NAir-N2 = 0.7 mUr) with that of each labelled salt (Cambridge Isotope Labs, 98% of labelled atoms) in the opportune mixing ratio, following the equations reported in [28].
At d 10, each of 30 potted seedlings for each labelling treatment was supplied with 50 mL of a 0.336 M ammonium nitrate solution (i.e. 470 mg of N per pot), corresponding to N requirements for a 180-days growing period, estimated according to previous reports on dynamics of leaf and root growth, N content and uptake efficiency [31–34]. In this way, it was possible to assess the labelling dynamics of plant materials since an initial starting point of known δ 15N of the nutrient solution.
Other macro- and micro-nutrient were also supplied at d 10, proportionally to N according to the following modified Hoagland solution (280 mL per pot): 10 mM MgSO4, 1 mM Fe(Na)-EDTA, 20 μM KCl, 0.5 mM H3BO3, 40 μM MnSO4, 40 μM ZnSO4, 2 μM CuSO4, 2 μM (NH4)6Mo7O24, 4 mM CaSO4, 20 mM K2HPO4, 60 mM K2SO4. Before supply, the nutrient solution was buffered with MES (2-(N-morpholino)ethanesulfonic acid, 40 mM) and pH was corrected at 6.0±0.1 with HCl 4M. After d 10, no further nutrient was administered to the pots, with the exception of CaSO4, since its low solubility made impossible to fulfill plant requirements with the initial dose at d 10. Therefore, CaSO4 was supplied (at 4 mM per pot) over the growing period while watering (see next section), for a total of 1.524 g/plant.
It is worth noting that, all together, the ion strength of the nutrient solutions was extremely high (over 260 mM), especially immediately after the nutrient supply at d 10. While this posed issues related to osmotic stress, preliminary tests had showed that B. napus seedlings were capable to survive such stressing conditions. Therefore, although possibly misrepresenting physiological conditions during plant growth in nature/field, our approach, with most nutrient supply at the beginning of the growing period was the only choice allowing to monitor the labelling dynamics of plant tissues and DNA at medium term (120 d).
Plant cultivation
In the growing room, pots were randomly (i.e. independent of the labelling treatment) placed onto five trolleys. Trolley within the room and pots within each trolley were daily and weekly moved, respectively, to keep homogeneous exposure condition among replicates. Water loss by evapotranspiration was reintegrated by watering the pots every two days with milli-RO water. At d 15, pot thinning by uprooting the less developed seedling allowed to maintain a replicate for each treatment while avoiding possible confounding effects of within-pot intraspecific competition.
Destructive sampling
Starting at d 60 and every 15 days until d 120, 6 pots per treatment and 2 control pots were randomly selected and plants uprooted. Roots were gently washed with deionized water in order to remove sand particles and residues of nutrient solution. Afterwards, roots, stems (petioles) and leaf materials of each plant were separated.
Fresh plant materials (i.e. leaves, stems and roots) of each sampled pot were separately weighted. Afterwards, aliquots (i.e. 15 mm-diameter discs, 2 cm-long segments and a portion of the tip for each leaf blade, stem and root, respectively) were collected, fresh-weighted, dried in stove (24 hrs. at 60°C), dry-weighted, pulverized (TissueLyser II, Qiagen, Hilden, Germany) and kept in sterile plastic tubes for CHN-IRMS analyses. Residual, fresh plant materials (5 g each) were separately ground (Mill A11 basic, IKA, Saint Louis, Missouri, USA) in liquid nitrogen (T = -196 °C), placed in sterile 50 mL Falcon tubes and stored in freezer at -80 °C for subsequent DNA extraction.
Since fresh plant materials are required for DNA extraction, the shoot: root ratio of each plant was determined as the ratio between the total dry leaf and stem biomass and the total dry root biomass of each sampled plant, with dry weights estimated based on the fresh weight: dry weight ratio of the aliquots.
DNA extraction, purification and quantitation
DNA extraction from fresh leaves and roots of each plant followed a modified version of the Doyle & Doyle protocol [35], as follows: a lysis buffer was prepared mixing 20 mL of CTAB (2.5%), a spatula tip of PVP-40, 2 μl of Proteinase K (20 μg/μl) and 200 μl of β-mercaptoetanol (0.1%). The buffer solution was kept in agitation in a thermostatic bath at 65 °C (pbi, Braski, Bergamo, Italy), until PVP complete dissolution. For each source plant material, 20 mL of the buffer solution were added to the Falcon tube and the mixture was incubated at 65 °C for 30 min. and successively cooled in ice for 10 min. DNA purification was performed adding 20 mL of a mixture of chloroform: isoamyl alcohol (24:1) and gently shacking for 10 min. to homogenize. Falcon tubes containing the mixture were centrifuged at 6800 rpm at 4°C for 30 min, then gently pipetting out the aqueous supernatant fraction. Sodium acetate (1/10 starting volume, 3M), NaCl (3/10 starting volume, 4M) and pure Isopropyl alcohol (2/3 final volume) were added to the collected supernatant. The solution was incubated at -20°C for 30 min. and successively centrifuged as described above. As final step, after removing the supernatant and twice washing the pellet with 2 mL ethanol (80%), the pellet was dried in a stove (10–15 min. at 37 °C) and re-suspended into an Eppendorf tube filled with 1.7 mL of sterile water for quantitation.
The concentration of extracted DNA in the resuspension medium was assessed by fluorimeter Qubit 3.0 (Life Technology, Carlsbad, California, USA). Finally, aliquots of 1.5 mL of each DNA sample were collected, frozen, lyophilized (55–4 Coolsafe, Scanvac, Allerød, Denmark) for 24 h (0.050 mbar, T = -57 °C) and kept in sterile plastic tubes at -20°C or subsequent CHN-IRMS analyses.
CHN-IRMS analysis
Dry, pulverized root and leaf samples as well as lyophilized DNA samples purified from the same materials were weighted at 2 ± 0.5 mg in cylindrical tin capsules (diameter 5 mm, height 9 mm) (Säntis Analytical AG, Teufen, Switzerland) in duplicates. A total of 800 replicated samples (100 plants x 2 plant materials x 2 technical replicates x 2 N pools, i.e. total N and DNA) were processed by elemental analysis/isotope ratio mass spectrometry (EA/IRMS), using a Vario Micro Cube (Elementar GmbH, Langenselbold, Germany) elemental analyzer connected online in continuous flow mode to an IsoPrime 100 (Elementar UK Ltd, Cheadle Hulme, UK) isotope ratio mass spectrometer, using helium (He) as a carrier gas.
Flash combustion of all samples was conducted at 950 °C with a pulse of O2 (30 ml/min for 70’) into the He carrier gas in a quartz combustion column prepared following the manufacturer instructions. From bottom to top, the column was filled with: quartz wool (two layers, each of height 2.5 mm, separated by a 18 mm- thick layer of quartz chips), silver wool (25 mm), quartz wool (5 mm), CuO (65 mm), corundum balls (3 mm), an ash-finger tube with Al2O3 bottom, and a sheath tube. The combustion gas products (CO2, N2, NOx and H2O) were passed through a reduction column at 500 °C to reduce the non-stoichiometric nitrous products to N2 and to remove excess oxygen from the gas stream. The reduction column was prepared following the manufacturer instruction and filled with quartz wool at the bottom (5 mm height), elemental copper (295 mm), silver wool (20 mm). The plug of the reduction column was also filled with silver wool, to bind volatile halogen compounds contained in the combustion gas products. Reduced gases were then dried by passing through a 10 cm glass column filled with anhydrous SICAPENT® (Merk, Darmstadt, Germany), then passing into desorption columns to absorb the measurable components of the analysis gas mixture and then release each of them by controlling the desorption temperatures. Once released, the gases sequentially passed through a Thermal Conductivity Detector (TCD) and were vented out to the Isoprime diluter (Elementar UK Ltd, Cheadle Hulme, UK) for diluting CO2 flow in the carrier helium flow at a rate of 100 ml/min before entering in the mass spectrometer. In parallel to this sample line, a second helium line is connected to the source of the mass spectrometer to carry the two calibration gases (CO2, N2). Isotopic measurements and data processing were performed with the software IonVantage (Elementar UK Ltd, Cheadle Hulme, UK).
The nitrogen stable isotope composition in a given sample can be reported in the delta (δ) notation as variations of the molar ratio (R) of the heavy (15N) to light isotope (14N) in the sample relative to molecular nitrogen in air (Air-N2) as international standard [36]. Accordingly, we used the following notation:
The unit commonly used to express the delta value is permil (‰). However, the use of ‰ is debated as in conformity with the International System of Units (SI) and according to the guidelines and recommendations of the International Union of Pure and Applied Chemistry (IUPAC)—Commission on Isotopic Abundances and Atomic Weights [37, 38], the unit of the delta values is the “urey” (symbol Ur). Therefore, we presented values of nitrogen isotopic composition with the unit notation mUr. However, as 1 mUr equals 1 ‰, for the sake of compliance with previous studies, we also presented isotopic composition values with the double unit notation of “mUr or ‰” limited to figures and tables, as often reported in the literature [e.g. 39, 40].
Analytical results for nitrogen isotopic composition were calibrated using sulphanilamide (Elementar GmbH, Langenselbold, Germany, N = 16.26%, C = 41.81%, 7 samples per batch) as a reference material. Analytical results for nitrogen isotopic composition were linearly corrected using the following international reference materials (International Atomic Energy Agency, Wien, Austria): IAEA-N1 (ammonium sulphate, δ15NAir-N2 = 0.4 mUr, 4 samples per batch): IAEA-305A (ammonium sulphate, δ15NAir-N2 = 39.8 mUr, 4 samples per batch), IAEA-310A (urea, δ15NAir-N2 = 47.2 mUr, 2 samples per batch), USGS 26 (ammonium sulphate, δ15NAir-N2 = 53.7 mUr, 2 samples per batch), IAEA-310B (urea, δ15NAir-N2 = 244.6 mUr, 4 samples per batch), IAEA-305B (ammonium sulphate, δ15NAir-N2 = 375.3 mUr, 4 samples per batch). Each analytic batch (120 positions) included 90 samples, 27 reference materials, 2 blanks consisting in empty tin capsules and 1 empty position. To avoid possible concerns of memory effects in the analytic results due to isotopically enriched samples, blanks were measured at the beginning of the batch and samples were sequentially placed in each batch according to the expected isotopic enrichment for different types of samples, thus minimizing the enrichment gaps between each sample type. Furthermore, duplicates of the same source sample were always placed in different batches to increase accuracy. The repeatability and intermediate precision of the EA/IRMS were determined by the standard deviation of separately replicated analyses and were better than 0.1 mUr.
Data analysis
All statistical analyses were performed using the software Statistica 7.0 (StatSoft inc., Tulsa, Oklahoma, USA). Generalized linear models (GLMs) were fitted for leaf, root and stem biomass and N content, considering main and interactive effects of the labelling treatment (three levels: ṄH4ṄO3, ṄH4, ṄO3), plant material (three levels: leaves, stems and roots) and plant age, the latter included in the model as a continuous covariate. For each plant material and age, significant differences between treatments and control plants were tested using Tuckey’s HSD post-hoc test.
In order to compare δ 15N of purified DNA to that of the source plant materials, we calculated a Normalized Difference Index (NDI) for each replicate and treatment combination (i.e. labelling treatment, plant material and age), as follows:
where i, j, k and n indicates the labelling treatment, the type of source plant material (either leaf or root), plant age and the experimental replicate (individual plant), respectively. As such, NDI values range between -1 and 1, corresponding to unlabelled (δ 15NAir-N2 = 0 mUr) DNA and source plant material, respectively, while NDI = 0 corresponds to equal δ 15N values of DNA and total N pool of the source plant material.
GLMs were fitted for δ 15N of DNA and source plant materials, and for NDI as well, considering main and interactive effects of the labelling treatment (three levels: ṄH4ṄO3, ṄH4, ṄO3), plant material (two levels: leaves, stems and roots) and plant age, the latter included in the model as a continuous covariate. For all GLMs, pair-wise significant differences between treatment combinations were evaluated by Tuckey’s post-hoc HSD test at α = 0.05. Limited to NDI data, mean values of different experimental groups (i.e. unique combinations of plant material, age and labelling treatment) were also tested for significant difference from the reference zero value by one-sample t test at α = 0.01, thus reducing the conventional level of statistical significance of 0.05 in order to control for multiple comparisons. As such, significant negative and positive NDI mean values were interpreted as indicating 15N depletion and enrichment in DNA, respectively, compared to the total N pool of the source plant materials.
Results
δ 15N dynamics in labelled plant leaves and roots
Labelling treatments did not affect plant growth (Fig 2, S3 Table), percent N content (Fig 2, S4 Table) and biomass allocation (S1 Fig, S6 and S7 Tables). Leaf, stem and root biomasses, as well as their percent N content, were not significantly different among labelling treatments and between them and the unlabelled control plants (S5 Table), with the exception of leaf biomass at 120 d, which was significantly lower in plants of the three labelling treatments (Fig 2 and S5 Table, Tuckey’s HSD test: p-values < 0.0001 in all three pairwise treatment vs. control comparisons), possibly due to the interplay of individual variability and low sample size of the control plants. Finally, labelling treatments did not affect shoot: root ratio (S1 Fig, S6 and S7 Tables).
Arrows on the left panels indicate the labelling administration date at plant age of 10 d. Data refer to mean of 6 plants for each treatment combination. Deviation bars are omitted to improve readability. Statistical support in S2, S3 and S4 Tables.
δ 15N of leaves and roots largely varied with plant age and among labelling treatments (Fig 3), as indicated by the significant interaction term in the GLM model (Table 1).
Right panels show the corresponding δ 15N Normalized Difference Index (NDI) dynamics. Data refer to mean of 6 plants for each treatment combination. Statistical support in Tables 1 and 2, and S8, S9 and S10 Tables.
In leaves, initially (60 d) δ 15N did not show significantly different values among ṄH4ṄO3, ṄH4 and ṄO3 plants. At the second observation stage (75 d), a slight increase in ṄH4 and a decrease in ṄO3 plants were detected (Fig 3), but still not statistically significant compared to the previous stage in both treatments (S8 Table). Since 75 d, δ 15N dynamics differed among the three treatments (Fig 3), with ṄH4 leaves showing a progressive decrease, down to -37.0% from 75 d to 120 d, while ṄO3 leaves showed the opposite pattern, increasing by 40.5% in the same time frame (S8 Table). Differently, ṄH4ṄO3 leaves did not show significant δ 15N changes along the observation period (S8 Table). Interestingly, after 120 d observation δ 15N in ṄO3 leaves significantly exceeded that of ṄH4ṄO3 ones by 31.1% and that of ṄH4 leaves by 61.5%, while the difference between the mean values of the two latter treatments was not statistically significant, with ṄH4 leaves showing high within-treatment variability (S8 Table).
δ 15N dynamics in roots were qualitatively similar to those observed for leaves (Fig 3). However, significant 2nd order interactive effects of the types of plant material and labelling treatment, and of the 3rd order interaction with plant age as well (Table 1), indicated that δ 15N values in plant roots, within each labelling treatment, followed quantitatively different dynamics compared to leaves. In particular, significant differences among ṄH4ṄO3, ṄO3 and ṄH4 roots were observed since the beginning of the observation period (S8 Table), with the latter treatment producing δ 15N values exceeding those of ṄH4ṄO3 and ṄO3 roots by 49.0% and 88.8%, respectively. Such trend still held at 75 d, with corresponding percent differences of 51.1% and 94.0%, respectively.
Thereafter, from 90 d to 120 d, root δ 15N dynamics were apparently similar to those observed in leaves (Fig 3), although ṄO3 roots did neither show statistically significant age-dependent variations, nor significant differences compared to the other treatments within each observation stage (S8 Table). The only exception to such pattern was the significantly higher δ 15N values at 120 d in ṄO3 vs. ṄH4 roots (+34.8%), resulting from the decreasing age-dependent trend observed in this latter treatment (-42.4% from 75 d to 120 d). As observed in leaves, ṄH4ṄO3 roots did not show significant δ 15N changes along the observation period (S8 Table).
Finally, δ 15N differences between root and leaf materials within each labelling treatment at each observation stage were not statistically significant with the exception of ṄH4 and ṄO3 plants at 60 d, showing higher and lower values in roots, respectively (S8 Table).
δ 15N dynamics in plant DNA
δ 15N of DNA purified from leaves and roots generally followed a similar pattern as compared to that observed in the source plant materials (Fig 3), resulting from the interaction of plant age and labelling treatment effects (Table 1), although with peculiar and interesting shifts along the observation period. In particular, in the case of leaf DNA, δ 15N of ṄH4 DNA was consistently higher compared to the other two treatments throughout the first 90 days of observation (S9 Table), exceeding that of ṄH4ṄO3 DNA by 34%, 49.9%, and 29.8% at 60, 75 and 90 d, respectively, and that of ṄO3 DNA by 50.6%, 80.9% and 43%, respectively. Interestingly, such differences were released at 105 d, with δ 15N of DNA from all treatments showing converging dynamics up to that point, with a shift in time in comparison to what was observed for the δ 15N of the source plant materials (Fig 3). Thereafter, at 105 d and 120 d, δ 15N of DNA apparently decreased in all treatments, although such trend was statistically significant limited to ṄH4 plants (S9 Table).
Different from leaf DNA, δ 15N dynamics in root DNA were much more similar to those observed for the source plant materials, with no significant within-treatment variation between 60 and 90 d, and ṄH4 DNA always showing higher levels compared to the other two treatments in the same time frame. In particular, δ 15N of ṄH4 DNA exceeded that of ṄH4ṄO3 DNA by 68.6%, 76.5%, and 43.7% at 60, 75 and 90 d, respectively, and that of ṄO3 DNA by 196.7%, 213.4% and 99.1%, respectively, corresponding to a larger magnitude of between-treatment variation compared to the source plant materials (Fig 3). From 90 d to 105 d, the δ 15N of ṄH4 DNA and ṄO3 DNA showed abrupt shifts corresponding to significant decrease and increase, respectively, while δ 15N did not significantly vary in ṄH4ṄO3 DNA (Fig 3, S9 Table). Such trends were released at the final observation stage (120 d), as none of the three labelling treatments produced significant variation compared to the preceding stage (105 d) (Fig 3, S9 Table).
Similar to what observed for the total N pools of plant materials, δ 15N differences between root and leaf DNA within each labelling treatment at each observation stage were not statistically significant, with the exceptions of ṄO3 DNA at 60 d and ṄH4 DNA at 60 and 75 d, showing lower and higher values in roots, respectively (S9 Table).
δ 15N NDI dynamics: DNA vs total N pool of the source plant materials
A comparative analysis of δ 15N dynamics in DNA samples and in the corresponding source materials can be better clarified by observing δ 15N NDI patterns (Fig 3), which significantly changed in relation to the labelling treatment, plant material, age and their interactions (Table 2). In the case of leaves, δ 15N NDI values were consistently negative throughout the observation period for ṄH4ṄO3 and ṄO3 plants, indicating that leaf DNA was always depleted in 15N compared to the source plant material (S10 Table). Such trend was found in ṄH4 leaves only at the final observation stage (120 d), while at the preceding stages δ 15N NDI values did not significantly differ from the reference zero value, thus indicating that δ 15N of ṄH4 DNA did not differ from that of the source plant material (S10 Table). Correspondingly, δ 15N NDI dynamics within each treatment did not show significant fluctuations up to the third or fourth observation stage (90 d or 105 d). Then, δ 15N NDI significantly decreased, with negative values consistently observed at 120 d in all treatments, indicating that 15N depletion in DNA at the final observation stage was independent of the labelling treatment.
δ 15N NDI dynamics in plant roots showed substantially the same pattern observed for leaves up to the first 90 d (Fig 3), with the only exception of ṄH4 roots showing a significant positive value at 90 d (S10 Table). Differently, at the final observation stages (105 d and 120 d) δ 15N NDI dynamics in plant roots showed a significantly different pattern compared to that observed for plant leaves, particularly in the cases of ṄH4ṄO3 and ṄO3 roots (S10 Table). Indeed, ṄH4ṄO3 roots persistently showed negative δ 15N NDI values, but without the decreasing trend observed in ṄH4ṄO3 leaves (Fig 3). ṄO3 roots showed an increasing trend (Fig 3), leading to δ 15N NDI values non-significantly different form the reference zero value (S10 Table). ṄH4 roots showed substantially the same pattern observed for ṄH4 leaves (Fig 3), with a significant decrease from 90 to 120 d corresponding to negative δ 15N NDI value at the final stage (S10 Table) indicating 15N depletion in DNA.
Considering δ 15N NDI values in roots and leaves within each treatment and observation stage, we found the only significant difference at the end of the observation period (120 d), with ṄH4ṄO3 and ṄO3 leaves showing lower values compared to the corresponding root materials (S10 Table), thus indicating that 15N depletion in DNA compared to the total N pool was larger in leaves than in roots.
Discussion
Effects of N uptake on δ 15N dynamics in leaf and root
We found that the isotopic composition of plant roots and leaves largely varied along the vegetative growth period, with early-to-medium dynamics corresponding to 15N enrichment and depletion in ṄH4 and ṄO3 plants, respectively, and with an opposite pattern at later stages. Such trend, more evident in roots compared to leaves, was independent of labelling treatments effects on plant growth and N content and biomass allocation dynamics. The substantially specular dynamics of δ 15N in ṄH4 vs. ṄO3 plants, clearly indicated that uptake fluxes of the two N chemical species were decoupled over time, with plants mostly using NH4+ up to an age of 90 days and NO3- afterwards. This is consistent with the expected outcomes, since the relative abundance and the isotopic composition of different chemical species in the substrate are the most controlling factors of plant δ 15N dynamics [30]. In this respect, it is worth noting that our experimental design is novel as compared to previous studies, where a single labelled N species and single harvesting shortly after the labelling treatment were used [4, 30, 41]. Differently, by adopting a factorial combination of different labelled N sources and harvesting plants over a prolonged observation period, we assessed the relative importance of uptake fluxes by comparing enrichment dynamics between ṄH4 and ṄO3 plants. Additionally, we could evaluate the associated isotopic fractioning by monitoring labelling dynamics in ṄH4ṄO3. As such, our results clearly confirm that the effects of the N source largely overcome that of isotopic discrimination during N uptake in controlling plant δ 15N dynamics.
Consistently, δ 15N did not significantly change over time in ṄH4ṄO3 roots and leaves, as not affected by the uptake dynamics of the two labelled N chemical species. Such finding may be surprising, as at least a slight increase over time in δ 15N of ṄH4ṄO3 plants, and particularly of their roots, would be expected as a result of isotopic discrimination associated to NH4+ and/or NO3- uptake [1], due to a progressive enrichment in 15N in the pot solution and hence in plant roots. The magnitude of isotopic discrimination (ε) associated to N uptake was previously estimated both for NO3- [13, 41, 42] and NH4+ [4, 30]. Reported values ranges between 0 (i.e. absence of discrimination) and -12,6 mUr [4, 30] in the case of NH4+, and between 0 and -9,6 mUr [30, 41, 42] for the uptake of NO3-. Such variability could be likely related to different experimental conditions considered in those previous studies. Among these, the target species and/or cultivar [7] and plant age [1] may play a major role, as well as N concentration in the substrate, which at low values triggers active uptake transporters [4, 8] and positively affects the magnitude of isotopic discrimination. Therefore, it is not surprising that we could not detect the occurrence of isotopic fractioning associated to N uptake, considering that we started to monitor δ 15N values in N-rich plants of 60 d.
Effects of N assimilation on δ 15N dynamics in leaf and root
We expected isotopic enrichment in ṄH4 roots compared to leaves and in ṄO3 leaves compared to roots at each observation stage, and intermediate values in ṄH4ṄO3 materials, due to differences in assimilation rates of the two inorganic N chemical species in the two plant organs.
NO3- is readily assimilated in the roots after uptake, being firstly reduced to NO2- by the Nitrate Reductase enzymatic complex and then to NH4+ by the Ferrodoxin-Nitrite reductase [8]. The first enzyme discriminates the heavy isotopic form [1, 9], with a generally accepted fractionation value of –16 mUr [11, 14]. Then, the NO3- available for assimilation in leaves, after xylematic transport, is enriched in 15N compared to that assimilated at root level [1]. Our results confirmed the expected pattern only for the first observation stage (60 d). This apparently contrasts with the enhanced content of assimilation enzymes at root level [43], which is expected to result in progressive enrichment of unassimilated NO3- that is then transported to the leaves. However, our results can be easily explained considering the remarkable N availability in the pot solution and the consequent low NO3- assimilation flux in the roots. In such conditions, biomass and N allocation were extremely unbalanced between roots and leaves. For instance, at a plant age of 75 d, mean N content values in ṄO3 leaves and roots were 111.8 mg and 3.9 mg, respectively, while the same values at 90 d were 177.4 mg and 12.3 mg. This corresponded to 65.6 mg N allocated to the leaves, while the net N allocation to the roots in the same time frame was one order of magnitude lower, equal to only 8.4 mg. Therefore, 15N enrichment in the leaves due to fractioning effects of NO3- assimilation in the roots [1] was likely lower than expected, as related to the low NO3- assimilation flux in the roots. In addition, a 15N enrichment in roots due to the backflow of nitrate to roots via the phloem, as recently suggested by a modelling work by [14], cannot be excluded.
In the case of NH4+ assimilation, N is firstly incorporated into organic molecules such as Glutamine and Glutamate in the roots [8, 44] through a three-stages assimilation process mediated by the GS-GOGAT enzymatic complex. Since the first stage of such process, mediated by the Glutamine Synthetase enzyme, discriminates the heavy N isotopic form [e.g. 12, 45], the organic products of the NH4+ assimilation transported to the leaves are depleted in 15N. Therefore, δ 15N in ṄH4 roots is expected to be higher than in ṄH4 leaves. Our results for ṄH4 plants were consistent with such expectation only at the first observation stage (60 d). Interestingly, at later stages, we found large within-group variability, particularly at 90 d. At this stage, δ 15N also did not differ among ṄH4 and ṄO3 materials, indicating that plant materials had acquired the same amount of NH4+ and NO3-, irrespective of the labelling treatment. Since at this stage slight variations in the uptake rates of either N source produces large δ 15N variations, the high within-group variability of δ 15N values, which prevented from detecting significant root vs. leaf differences, could be ascribed to a certain asynchrony in labelling dynamics among replicates within each treatment. Finally, a possible role of ammonia volatilization, at least for some replicates, and its effect on δ 15N dynamics cannot be excluded [12]. It is well known that ammonia volatilization rates increase with temperature and with leaf N content, particularly during senescence [46, 47]. In our experiment, mild temperatures and absence of senescence processes likely contributed to limit ammonia volatilization. On the other hand, the remarkable percent N content in plant leaves up to the end of the observation period, might have enhanced N loss by volatilization.
15N depletion dynamics in plant DNA
Following Gauthier et al. [20], we hypothesized that isotopic fractioning along the purine and/or pyrimidine biosynthesis pathways leads to a depletion of 15N in plant DNA, hence expecting lower δ 15N values in leaf and root DNA samples compared to those of the source plant materials.
Dynamics of δ 15N values in DNA samples, decreasing and increasing in ṄO3 and ṄH4 treatments, respectively, were substantially consistent to those of the source materials up to 105 d. Then, at the final observation period, δ 15N dynamics in all DNA samples were completely different from that of the source materials, with a consistent decrease in most cases. Dynamics of δ 15N NDI provided a clue to explain such pattern, consistently showing negative values for ṄH4ṄO3 and ṄO3 (i.e. 15N depletion in DNA samples compared to the total N pool of the source plant materials) for both leaves and roots. For such treatments, our findings fully support the occurrence of isotopic fractioning along the purine and/or pyrimidine biosynthesis pathways [20]. On the other hand, δ 15N NDI dynamics observed in the case of ṄH4 leaves and roots, showed values not significantly different from zero (i.e. equal δ 15N in DNA samples and the source plant materials up to 105 d), and even a positive value for roots at 90d. Such result may be explained considering the interplay of isotopic fractioning during DNA biosynthesis, N uptake and assimilation in the three labelling treatments. Up to 90 d, the negative δ 15N NDI values of ṄH4ṄO3 plants, as not affected by the labelled N species, relied only on 15N depletion in DNA. Differently, δ 15N NDI values were significantly different between ṄO3 and ṄH4 plants, for both leaves and roots. This indicates that the progressive isotopic enrichment and depletion in ṄO3 and ṄH4 leaves and roots, respectively, were exacerbated in DNA samples compared to the source materials. Moreover, the increase and decrease of δ 15N values in ṄO3 and ṄH4 DNA samples, respectively, were delayed compared to the corresponding plant materials. This may be attributed to a temporal decoupling of N incorporation in purine and pyrimidine precursors with respect to nucleotide assemblage into DNA molecules, with 15N signature of DNA samples at a given stage reflecting that of the source material at previous stages. Accordingly, in the case of ṄH4 DNA samples, the effect of isotopic fractioning in purine and pyrimidine biosynthesis [20] might have been masked by labelling dynamics of the source materials.
After 90 d, δ 15N NDI values were consistently negative, and mostly decreasing, for leaves of all treatments, confirming the occurrence of 15N fractioning during leaf DNA biosynthesis [20]. The results for root materials was less straightforward, for different possible reasons. First, root growth rate between 105 and 120 d was one order of magnitude smaller compared to leaves (mean and standard deviation of all treatments was kB = 0.036 ± 0.011 gDW d-1 and kB = 0.353 ± 0.020 gDW d-1 for roots and leaves, respectively). In these conditions, DNA biosynthesis rate was higher in leaves, compared to roots and to the preceding stages, hence magnifying the effect of isotopic fractioning associated to purine and pyrimidine biosynthesis. Second, in the same observation period (between 105 and 120 d) mean daily increases of N mass in leaves (kN = 0.58 ± 0.1 mgN d-1) and roots (kN = 0.18 ± 0.03 mgN d-1) showed more similar magnitude as compared to the corresponding kB values. Hence, at this stage, the utilization efficiency [48] of newly up taken N (i.e. kB / kN) in the leaves (i.e. 0.222 gDW mgN-1) was larger compared to the roots (0.077 gDW mgN-1) and to the preceding stages (i.e. 0.012, 0.013, 0.024 and 0.031 gDW mgN-1 at 60, 75, 90 and 105 d, respectively), with DNA bases biosynthesis likely relying more on this N pool rather than that previously taken up but not used and stored in reserve pools such as vegetative proteins [32]. Finally, we cannot exclude a possible and decisive role of N translocation from leaves to roots [44], which however should have played the major role at the initial stages of observation, when percent N content in leaves was far higher than physiological values commonly reported [e.g. 32].
Conclusion
In this study we confirmed previous evidence on the effect of the labelled chemical species on leaf and root δ 15N dynamics. Under the tested conditions, higher uptake rate of NH4+ and its limiting effect on the uptake of NO3- were the main causal factors of the observed outcomes, with ṄH4, ṄO3 and ṄH4ṄO3 plants initially showing higher, lower and intermediate δ 15N values, respectively, then progressing towards the opposite trend when NH4+ depletion from the nutrient solution corresponded to increasing NO3- uptake rate.
Although it is well known that isotopic fractionation during inorganic N uptake, associated to 15N enrichment of the N pool residual in the substrate solution, results in progressive isotopic enrichment of plant tissues, our study did not provide conclusive results, even in the case of ṄH4ṄO3 plants, unaffected by uptake rates of the two chemical species. However, possibly unsuitable experimental conditions, in terms of excessive N availability, might have hampered active inorganic N uptake mechanisms, decisively affecting our observations.
Evidence from previous studies on leaf vs. root isotopic enrichment due to enzymatic fractionation during inorganic N assimilation were only partially confirmed, limited to ṄO3 plants at the early observation stages. At later stages and for ṄH4+ plants, the predominant effects of NH4+ and NO3- uptake rates in the tested conditions, as well as the reduced root development and the extremely high leaf N content, with the associated possible confounding effects of nitrate phloematic backflow and ammonia volatilization, likely masked the expected outcome.
Considering the hypothesis of 15N depletion in DNA compared to the source plant materials, possibly due to enzymatic discrimination during purine biosynthesis, our findings provide confirmatory evidence. However, we did not provide a direct evidence of δ 15N variation between molecular products such as nuclei acids and their precursors according to known biochemical pathways. Indeed, addressing such issue would require more detailed characterization of the involved N molecular pools and additional experiments to accurately estimate the fractionation coefficient of each enzymatic step during DNA biosynthesis. However, as an added value of our original experimental design, we were able for the first time to specifically report about the dynamics of specific plant molecular pools, such as leaf and root DNA, over a long observation period.
Supporting information
S1 Fig. Shoot: Root ratio changes of in B. napus plants over the observation period, across the labelling treatments.
Different letters above bars indicate significant pair-wise labelling-dependent differences at equal plant age (Tuckey’s post-hoc test after two-ways ANOVA, S6 and S7 Tables).
https://doi.org/10.1371/journal.pone.0247842.s001
(TIF)
S1 Table. Physical-chemical features of the quartz sand substrate used for potting.
https://doi.org/10.1371/journal.pone.0247842.s002
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S2 Table. Results of GLM for B. napus biomass and N percent content.
https://doi.org/10.1371/journal.pone.0247842.s003
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S3 Table. Result of Tuckey’s post-hoc HSD testing for the interactive effect of plant age and labelling treatments (ṄH4ṄO3, ṄH4, ṄO3) on dry biomass of B. napus leaves, stems and roots.
Data refer to mean ± standard deviation of dry weight (g) of 6 plants for each treatment combination. Different letters indicate significantly different groups within each plant material (P < 0.05).
https://doi.org/10.1371/journal.pone.0247842.s004
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S4 Table. Result of Tuckey’s post-hoc HSD testing for the interactive effect of plant age and labelling treatments (ṄH4ṄO3, ṄH4, ṄO3) on percent N content in B. napus leaves, stems and roots.
Data refer to mean ± standard deviation of N content (%) of 6 plants for each treatment combination. Different letters indicate significantly different groups within each plant material (P < 0.05).
https://doi.org/10.1371/journal.pone.0247842.s005
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S5 Table. Leaf, stem and root biomass and percent N content in the unlabelled control plants at the five observation stages.
https://doi.org/10.1371/journal.pone.0247842.s006
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S6 Table. Result of two-ways ANOVA testing for main and interactive effects of plant age and labelling treatment (ṄH4ṄO3, ṄH4, ṄO3) on the shoot: Root ratio of B. napus plants.
https://doi.org/10.1371/journal.pone.0247842.s007
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S7 Table. Result of Tuckey’s post-hoc testing for the interactive effect of plant age and labelling treatment (ṄH4ṄO3, ṄH4, ṄO3) on the shoot: Root ratio of B. napus plants.
https://doi.org/10.1371/journal.pone.0247842.s008
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S8 Table. Result of Tuckey’s post-hoc HSD testing for the interactive effect of plant age and labelling treatments (ṄH4ṄO3, ṄH4, ṄO3) on N isotopic composition of B. napus.
Data refer to mean ± standard deviation of 6 plants for each treatment combination. Different letters indicate significantly different groups within each plant material (P < 0.05). Significantly different values between leaf and root within each combination of labelling treatment and plant age are indicated in bold.
https://doi.org/10.1371/journal.pone.0247842.s009
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S9 Table. Result of Tuckey’s post-hoc HSD testing for the interactive effect of plant age and labelling treatments (ṄH4ṄO3, ṄH4, ṄO3) on δ 15N of B. napus leaf and root DNA.
Data refer to δ 15N mean ± standard deviation of 6 plants for each treatment combination. Different letters indicate significantly different groups within each plant material (P < 0.05). Significantly different values between leaf and root within each combination of labelling treatment and plant age are indicated in bold (*: DNA purified from root materials was pooled in order to provide the minimum sample amount for IRMS analysis).
https://doi.org/10.1371/journal.pone.0247842.s010
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S10 Table. Result of Tuckey’s post-hoc HSD testing for the interactive effect of plant age and labelling treatments (ṄH4ṄO3, ṄH4, ṄO3) on δ 15N NDI.
δ 15N NDI indicates differences of isotopic composition between leaf or root DNA and the total N pool of the source plant material. Data refer to δ 15N NDI mean ± standard deviation of 6 plants for each treatment combination. Different letters indicate significantly different groups within each plant material (P < 0.05). Mean values significantly different from zero, as assessed by one sample t-tests at P < 0.01, are marked with an asterisk (*). Significantly different values between leaf and root within each combination of labelling treatment and plant age are indicated in bold.
https://doi.org/10.1371/journal.pone.0247842.s011
(PDF)
Acknowledgments
We are grateful to Nicoletta Felice and Giusi Zaina for technical assistance during experimental work.
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