Biochemical characterization of the Nocardia lactamdurans ACV synthetase

Riccardo Iacovelli; Reto D. Zwahlen; Roel A. L. Bovenberg; Arnold J. M. Driessen

doi:10.1371/journal.pone.0231290

Abstract

The L-δ-(α-aminoadipoyl)-L-cysteinyl-D-valine synthetase (ACVS) is a nonribosomal peptide synthetase (NRPS) that fulfills a crucial role in the synthesis of β-lactams. Although some of the enzymological aspects of this enzyme have been elucidated, its large size, at over 400 kDa, has hampered heterologous expression and stable purification attempts. Here we have successfully overexpressed the Nocardia lactamdurans ACVS in E. coli HM0079. The protein was purified to homogeneity and characterized for tripeptide formation with a focus on the substrate specificity of the three modules. The first L-α-aminoadipic acid-activating module is highly specific, whereas the modules for L-cysteine and L-valine are more promiscuous. Engineering of the first module of ACVS confirmed the strict specificity observed towards its substrate, which can be understood in terms of the non-canonical peptide bond position.

Citation: Iacovelli R, Zwahlen RD, Bovenberg RAL, Driessen AJM (2020) Biochemical characterization of the Nocardia lactamdurans ACV synthetase. PLoS ONE 15(4): e0231290. https://doi.org/10.1371/journal.pone.0231290

Editor: Israel Silman, Weizmann Institute of Science, ISRAEL

Received: December 11, 2019; Accepted: March 19, 2020; Published: April 10, 2020

Copyright: © 2020 Iacovelli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the manuscript and its Supporting Information files.

Funding: We declare an affiliation with DSM Biotechnology (Delft, The Netherlands) for the period of the study. DSM Biotechnology provided support in the form of salary for author R. A. L. Bovenberg, who is both associated with the University of Groningen and the company, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of the author are articulated in the ‘author contributions’ section.

Competing interests: The authors confirm that author R. A. L. Bovenberg receiving salary from DSM Biotechnology does not alter adherence to PLOS ONE policies on sharing data and materials. Furthermore, there are no relevant patents, products in development or marketed products to declare.

Introduction

Nonribosomal peptides (NRP) represent a very versatile group of low to medium molecular weight compounds that exhibit various biological activities. These peptides are exclusively produced by nonribosomal peptide synthetases (NRPS) and do not only contain proteinogenic amino acids, but may also contain a wide variety of non-proteinogenic amino acids and hydroxy acids [1]. NRP often undergo a series of modifications in cis, whether through the action of the NRPS or by further tailoring enzymes.

NRP synthesis universally starts in every module with the adenylation (A) domain, serving as a highly selective gate keeper, which recruits and adenylates a specific substrate, thereby forming an acyl-adenylate conjugate. Subsequently, the substrate-conjugate is transferred to the thiolation (T) domain by means of the phosphopantetheine (ppant) arm, with the AMP being released. The ppant arm is a CoA (Coenzyme A)-derived cofactor, covalently attached to a highly conserved residue of serine of the T domain by a ppant-transferase. The activated substrates are then transported to the donor and acceptor sites of the up- or downstream condensation (C) domains, where peptide formation occurs with the upstream substrate being released from the ppant moiety, and the newly synthesized intermediate ready to be transported to the next condensation domain [2–6]. In some cases, the growing peptide can be further modified by accessory domains, such as N-methylation, substrate epimerization, and heterocyclization domains [7][8]. When the synthesis is completed, the peptide is often released via the activity of a thioesterase (Te) domain, either by macrocyclization or hydrolysis, resulting in a cyclic or a linear NRP, respectively [8][9].

Despite extensive efforts, including the solution of sub-domain, domain, di-domain and entire modular structures [5,10–14], the high conformational dynamics and flexibility that characterize NRPS enzymes [15] have rendered structural analysis a considerable challenge. Only recently the first structures of a dimodular NRPS were obtained [16], providing crucial information on the dynamics of inter-domain and inter-module interactions and, ultimately, NRP synthesis.

Due to the relative simplicity and overall significance, the β-lactam production pathway [17–22] has been a paradigm for related research fields. Three distinct enzymatic steps are involved in the production of β-lactams, with the trimodular NRPS L-δ-(α-aminoadipoyl)-L-cysteinyl-D-valine synthetase (ACVS) providing the tripeptide (L,L,D)-ACV as the precursor for β-lactam antibiotics, such as penicillins or cephalosporins (Fig 1). The three amino acids L-α-aminoadipic acid (L-α-Aaa), L-cysteine and L-valine are inserted in the final product in a co-linear fashion, thus the position of the incorporated substrate corresponds to the position of the respective module within the primary NRPS sequence [23]. Peptide formation itself is strictly determined by the selectivity of the domains of the ACVS. Furthermore, L-α-aminoadipic acid is adenylated on the δ-carboxyl group, resulting in a non-canonical peptide bond formation between L-α-Aaa and L-Cys. Lastly, L-valine is epimerized via an intrinsic epimerization (E) domain, located in the third module. The final product is released as a linear tripeptide, (L,L,D)-ACV, by a Te domain, and it is subsequently converted to isopenicillin N by the enzyme isopenicillin N synthase (IPNS), which catalyzes the formation of the β-lactam ring.

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Fig 1. ACVS domain organization and product formation.

The ACVS consists of a total of 10 domains arranged in three modules with distinct specificities for the incorporation of L-α-aminoadipic acid (L-α-Aaa), L-cysteine and L-valine into the tripeptide δ-(L-α-aminoadipoyl)-L-cysteinyl-D-valine ((L,L,D)-ACV). The domain arrangement is conserved [30] and follows the order: AT-CAT-CATETe. The resulting (L,L,D)-ACV is converted into isopenicillin-N (IPN) by the isopenicillin-N synthetase (IPNS). Following different biosynthetic routes, IPN can further be converted into penicillins, cephalosporins, cephamycins and related compounds. In the circle, a schematic representation of the strategy [33] adopted to engineer the specificity of the first module of Nl ACVS is shown.

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ACVS served as a model NRPS, aiding to establish the nonribosomal route of peptide production. The protein has been studied in filamentous fungi such as Penicillium, Aspergillus, Cephalosporium as well as bacterial Nocardia and Streptomyces species [24–30]. The partial biochemical reactions of the NRPS domains have been examined mostly by using crude cell extracts or partially purified enzyme [30]. The large size of the protein, 404–425 kDa [30], makes expression and purification challenging for biochemical characterization. Here, we focus on the pcbAB gene of Nocardia lactamdurans that encodes a 404 kDa ACVS, first enzyme of the cephamycin biosynthetic pathway in this organism [29]. This protein was previously overexpressed in Streptomyces lividans, purified to near homogeneity and characterized for ACV synthetase activity. The enzyme activity was measured using ¹⁴C‐valine in an ATP/PP_i exchange assay [31]. Here, we heterologously overexpressed Nl ACVS in E. coli HM0079 [32], a platform strain that carries the 4′-phosphopantetheine transferase gene sfp, crucial for the production of active holoenzymes. The protein was purified to homogeneity and characterized for tripeptide production and substrate promiscuity via HPLC-MS. This allowed for the determination of fundamental biochemical parameters and substrate specificity of the individual modules. Furthermore, we engineered the adenylation domain of the first module of ACVS, adapting a subdomain swap strategy [33][34] with the goal of generating hybrid NRPSs able to activate alternative substrates and incorporate these at the first position of the tripeptide for novel β-lactam production.

Results

Purification and biochemical characterization of the N. lactamdurans ACVS

The Nocardia lactamdurans ACVS was overexpressed in E. coli HM0079 as a C-terminal 6xHis-tagged protein, and purified by Ni²⁺ affinity purification. The overall yield was 13.9 ± 3.4 mg pure ACVS per liter of culture (Fig 2). The purified enzyme (fraction E3) was subjected to in vitro product formation assays using conditions outlined in the methods section. In these assays, varying concentrations of the three substrate amino acids were used (Fig 3). Reactions were evaluated over a 4 h time course, and analyzed by HPLC/MS. Resulting ACV levels were quantified and normalized, showing a near linear product formation over the entire time course (Fig 3A–3C). Maximal ACV product levels under the given conditions reached nearly 50 μM, exceeding the enzyme concentration (0.17 μM) by almost three orders of magnitude, indicating multiple turnovers. The calculated V_max value for the ACVS activity was 0.78 ± 0.14 μM (ACV)*min^-1*μM enzyme^-1. Apparent K_M values were determined from the Michaelis-Menten kinetics with a >98% curve fit. Values of 640 ± 16, 40 ± 1 and 150 ± 4 μM were obtained for L-α-aminoadipic acid, L-cysteine and L-valine, respectively (Fig 3D).

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Fig 2. Ni²⁺ affinity chromatography purification of the Nocardia lactamdurans ACVS.

Nl ACVS was isolated from E. coli HM0079 cells and harvested after overnight expression at 18 ºC. A cell-free lysate was obtained through sonication and subsequently separated into a clear supernatant (CFL) and the pellet was resuspended in 8 M Urea (CFL (i)). The clear lysate was further purified using gravity flow in combination with a His-tag affinity chromatography, using two washing steps (W1, W2) and elution with 50, 150 and 250 mM imidazole, respectively (E1, E2, E3). Marker lane shows reference proteins corresponding to 170 and 130 kDa.

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Fig 3. Enzymatic characterization of ACVS.

Three reaction series were conducted using various concentrations of L-α-Aaa (A), L-Cys (B) and L-Val (C) and analyzed by LC/MS to quantify the amounts of the ACV tripeptide produced for Michaelis-Menten kinetics (D).

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Substrate specificity of the Nl ACVS

Next, we determined the enzyme substrate specificity. Therefore, three sets of reactions were arranged, varying the substrate for each of the three ACVS modules, also using structural analogues. The concentration of the variable amino acid was set at 5 mM. Product levels were determined as end points after 4h and analyzed for formation of the predicted tripeptides and related structures using HPLC/MS (Fig 4). In addition to the three native substrates, 17 analogues were tested in a total of 25 reaction setups (Table 1). Next to the ACV tripeptide, we detected 11 of the expected tripeptides (M1: 3; M2: 5; M3: 3) as well as the AC-Cys tripeptide in a reaction using L-α-Aaa and L-Cys only (S1 Appendix). Based on extracted ion count, production levels vary strongly for the various tripeptides, from 0.003% up to 13.8% relative to ACV production levels, assuming the same degree of ionization for the alternative tripeptides, for which no chemical standard was available (Table 1).

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Fig 4. Substrate promiscuity of the N. lactamdurans ACVS: Structures of expected tripeptides.

The predicted structures of the novel tripeptides and their corresponding reactions are numbered 1–26 (see Table 1). Italic numbers indicate production of the respective tripeptide. * = ACC tripeptide derived from L-α-Aaa and L-Cys only (26).

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Table 1. ACVS substrate promiscuity.

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Three sets of reactions were analyzed varying the amino acid on one position within the tripeptide. Alternative substrates were added to a concentration of 5 mM, replacing either L-α-Aaa (M1), L-Cys (M2) or L-Val (M3). Reactions (numbered 1–26) were evaluated using LC/MS and peaks of interest were assessed according to accurate monoisotopic mass (Mi). The resulting levels were set relative to the production of ACV (= 100), assuming similar ionization. Values derived from two biological and technical replicates ± standard deviation (except for DL-aminopimelic acid and 2-oxoadipic acid reactions, with only two technical replicates).

Engineering of the first adenylation domain of Nl ACVS

Next, we constructed a set of hybrid NRPS enzymes, by replacing the L-α-aminoadipic acid-specific subdomain of the first adenylation domain of Nl ACVS with alternative amino acid sequences from donor NRPSs (S1 Fig). Herein, the word subdomain is used to define specific regions of the adenylation domains which meet distinct criteria, determined by Kries and coworkers [33]. Donor NRPSs were selected according to their substrate specificities: the alternative sequences were chosen to explore the possibility to engineer hybrids that would activate and incorporate amino acids with different types of side chains (L-glutamic acid, L-aspartic acid, L-threonine, L-leucine, L-tyrosine and L-valine). One of the subdomains (L-valine-specific) was selected from the third module of Nl ACVS itself. We further included the L-α-aminoadipic acid-specific subdomain from the first module of Penicillium chrysogenum ACVS, with a subdomain sequence identity of 48.5%, as a control. The complete set of subdomains used in the engineering strategy is listed in Table 2. The amino acid sequences encoding the substrate-specific subdomains were identified through multi-sequence alignment analysis, as outlined in the methods section and in Fig 5. Hybrid NRPS constructs were built using a system (S2 Fig) based on the Golden Gate assembly, a synthetic biology method that allows easy and seamless assembling of smaller DNA fragments into larger genes [34]. All the intermediate vectors utilized in the assembly strategy were successfully cloned in E. coli DH5α and sequenced individually. The Golden Gate assembly reactions for the hybrid NRPSs, which were named ECVS, DCVS, TCVS, LCVS, YCVS, VCVS and PcANlCVS (according to the predicted specificity of the hybrid module), were performed in vitro and transformed directly into E. coli DH5α cells for storage and sequencing.

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Fig 5. Subdomains multi-alignment and boundaries determination.

(A) Multi-alignment of the subdomains used in the engineering strategy proposed by Kries and coworkers [33]. The two residues highlighted in yellow in the consensus sequence represent the first highly conserved motif with a phenylalanine and the aspartic acid which forms a hydrogen bond with the α-amino group of the amino acid substrate [35]. The motifs highlighted with the red boxes represent the conserved motifs identified as boundaries of the subdomains in the consensus sequence. (B) Multi-alignment of the subdomains selected for this work; in absence of structural information the conserved motifs at both ends were used to determine the subdomain boundaries.

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Table 2. Set of subdomains selected for the engineering strategy.

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Hybrid NRPS genes were overexpressed in E. coli HM0079 as C-terminal 6xHis-tagged proteins, and purified by Ni²⁺ affinity purification as described for the wild-type Nl ACVS. The overall yield was slightly lower compared to the Nl ACVS, ranging between 4 and 9 milligrams of pure protein per liter of culture (Fig 6A). Next, the purified hybrids were subjected to in vitro product formation assays including the amino acid substrates required for product formation. The full HPLC/MS chromatograms were analyzed and filtered for the m/z values specific to the predicted tripeptides (S3 Fig). Only in one case we detected the production of the expected compound. The hybrid PcANlCVS produced the ACV tripeptide, though at considerably lower levels compared to the wild-type Nl ACVS (Fig 6, panels B and C).

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Fig 6. Overexpression and activity assays of the hybrid NRPSs.

(A) SDS-PAGE analysis of the purified fractions of the hybrids: only fraction E2 (150 mM imidazole) is shown. Marker lane shows reference protein corresponding to 180 kDa. (B) In vitro tripeptide production assays with hybrid enzymes. (C) The production of the tripeptide ACV was confirmed by accurate monoisotopic mass and equal retention time compared to the wild-type enzyme product and an ACV synthetic standard.

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Discussion

Here we report on the characterization of the Nocardia lactamdurans ACV synthetase, heterologously overexpressed in E. coli HM0079 [32] and purified to homogeneity. The aforementioned E coli strain contains a genomic copy of sfp, a phosphopantheteinyl transferase, essential to activate the apo-ACVS to its active holo-form. An efficient purification process was developed to obtain highly pure enzyme. Initial enzymatic characterization was performed by following the production of the tripeptide ACV. With respect to the three ACVS modules, distinct differences in substrate affinities were noted with the initiating L-α-aminoadipic acid module showing the lowest affinity for its substrate. Previous studies on the formation of product intermediates and partial reactions of NRPS enzymes suggest that the initial amino acid thiolation reaction is a rate limiting step in the assembly of nonribosomal peptides, necessary for the subsequent domains to adopt their distinct conformations for peptide bond formation and product release [6][36]. Overall, substrate affinities levels appear to be in line with other ACVS homologues, in particular prokaryotic enzymes [37].

We furthermore determined the substrate specificity of the ACVS modules within the context of the complete enzyme, by assessing the production of predicted tripeptides (Table 1 and Fig 4). Some ACVS homologues [31,38–40] exhibit a certain degree of substrate tolerance, despite considerably tight control mechanisms that assure correct product formation. With the N. lactamdurans ACVS, replacement of L-α-aminoadipic acid by substrate analogues yielded only trace amounts of Glu-CV, previously reported [38,40], and API-CV and OAA-CV tripeptides, while none of the other substrates tested were incorporated. The structures of the three analogues activated strongly resemble that of L-α-Aaa, thus indicating a strict specificity for module 1. With the second module (L-cysteine), two alternative tripeptides A-Ala-V and A-Leu-V were generated in substantial amounts while trace amounts of three other A-X-V tripeptides were found (Table 1 and Fig 4). This suggests that this module is more promiscuous. Finally, for the third module (L-valine), two novel products AC-Leu and AC-Ile were observed at a level of 0.5–1%, while AC-NorVal was found at levels over 13% relative to ACV. Module 3 shows some tolerance towards side chain length and the distribution of methyl-groups. However, substrates with hydroxyl or thiol groups are not incorporated. In addition, in the absence of L-valine, we observed the production of a putative AC-Cys tripeptide up to a level of 13%. While these results indicate that the individual modules can accept only certain substrates, it cannot be entirely excluded that some tripeptides are not synthesized because of weaker activity towards the alternative substrate in downstream activities, such as the condensation reaction.

Modules 2 and 3 of the Nl ACVS exhibit some degree of tolerance towards substrates that are structurally similar to the native ones, yielding significant levels of tripeptides. In marked contrast, module 1 accepted only three alternative substrates, L-Glu, DL-aminopimelic acid and 2-oxoadipic acid, resulting in the production of trace amounts of tripeptides. With L-Asp and DL-aminosuberic acid, no tripeptide could be detected. Considering the strong similarity of these structures with L-α-Aaa, it seems very clear that the length of the side chain is of crucial importance. The presence of the α-NH₂ group appears to be crucial as well, as only trace amounts of tripeptide were detected in the reaction with 2-oxoadipic acid, while adipic acid was not incorporated at all (Table 1).

Importantly, in the structure of ACV, the peptide bond between the first two amino acids occurs between the δ-carboxyl group of L-α-Aaa and the amino group of L-Cys (Fig 1). Thus, L-α-Aaa must be adenylated on the side chain, in contrast with the canonical mechanism of activation of the C-α carboxyl group of amino acid described for other bacterial NRPSs [35][41]. Nonetheless, module 1 remains the most interesting target for potential engineering approaches, as the cysteine and valine are essential for β-lactam ring formation, while the aminoadipate is exchanged with other moieties in the (semi-) biosynthetic pathway of penicillins. Theoretically, by achieving activation and incorporation of alternative substrates at the first position, novel compounds with antibiotic activity could be generated.

Therefore, we proceeded with engineering the specificity of the adenylation domain of module 1. Several strategies have been used in the past to engineer NRPS enzymes with the goal of producing modified compounds, amongst which subdomain-, domain- and full module- exchanges, active site modification and directed evolution [33,42–47]. Herein, we designed a strategy based on the Golden Gate assembly method [34] and adapted from the work of Kries and coworkers on the Phe-specific GrsA initiation module of gramicidin S synthetase [33]. Using this strategy, we successfully generated 7 hybrid NRPS genes that could be expressed in E. coli HM0079 and purified. The peptide production assays and LC-MS analyses, however, revealed that only one of the hybrids was able to produce the expected tripeptide in an in vitro reaction, though at much lower levels. More specifically, the hybrid PcANlCVS, with the same specificity as the native Nl ACVS, but with the subdomain region “implanted” from its Penicillium chrysogenum homologue.

While the difference in amino acid sequence between the fungal and bacterial ACVS was not a limiting factor, the narrow substrate range of the native enzyme seems to pose a greater obstacle to the engineering of functional NRPS hybrids with alternative specificities. Therefore, such engineering approaches could prove more successful when targeting naturally promiscuous enzymes. Recently it was reported that condensation domains also show specificity towards upstream activated substrate [48][49], and therefore exert an extra gate-keeping function. Thus, the chemistry of the ACVS reaction, the tight specificity shown by the first adenylation domain and its noncanonical interaction with the substrate, as well as the possibility of a second gate-keeping checkpoint on the condensation domain of module 2, all present significant challenges to the engineering of a functional hybrid ACVS capable of producing alternative tripeptides. Additionally, until the intra-NRPS reaction dynamics, conformational timing and structural organization of a multi-modular NRPS enzymatic system have been elucidated, global engineering efforts will remain challenging for this class of enzymes.

Materials and methods

Strains, plasmids and general culturing conditions

All cloning procedures were performed using E. coli DH5α. Cultures were grown using LB medium at 37 ^oC and 200 rpm and antibiotic selection was conducted utilizing 25 μg/mL Zeocin. The Nocardia lactamdurans pcbAB was cloned using an intermediate gateway vector and was subsequently sub-cloned into the pBAD-plasmid (pBR322 ori; araC; pBAD, Zeo^R) using SbfI x NdeI sites and including the introduction of a 6xHis-tag on the C-terminal end. This construct was kindly provided by DSM Sinochem Pharmaceuticals (now Centrient Pharmaceuticals). The synthetic DNA fragments encoding the donor subdomains were designed in silico and purchased from Invitrogen (GeneArt Strings). In silico PCR and cloning procedures, as well as subsequent analyses, were performed using the SnapGene® software (from GSL Biotech; available at snapgene.com).

Identification of swapping partners and multi-alignment analysis

Donor NRPS were identified using the database NORINE [50]. For each of the substrate specificities that were selected for the engineering strategy (L-glutamic acid, L-aspartic acid, L-threonine, L-leucine and L-tyrosine), the database was searched using the function “monomer search”. The resulting hits were further selected based on sequence identity to the first adenylation domain of Nl ACVS, the size of the subdomains (determined according to the criteria described previously by Kries et al. [33]) and the identity between the putative donor subdomains and the L-α-aminoadipic acid-specific subdomain (S1 Fig). All alignment analyses were performed using the software MEGA7 [51] and Unipro UGENE [52]. The sequences of the complete adenylation domains from the donor NRPS were all aligned to the sequences of the subdomains designed and utilized by Kries and coworkers, to identify conserved motifs and define subdomain boundaries (Fig 5). All the DNA fragments were designed in silico, with the addition of the appropriate restriction sites, using the SnapGene® software. The DNA fragments were subsequently synthesized and purchased from Invitrogen (GeneArt Strings). The L-Val specific subdomain from Nl ACVS M3 and the L-α-Aaa-specific subdomain from Pc ACVS M1 were amplified via PCR from pBAD-Nl ACVS and P. chrysogenum DS47274 gDNA, respectively. KAPA HiFi HotStart ReadyMix (Roche) was used, according to the manufacturer’s protocol.

Engineering of the first adenylation domain of Nl ACVS via a Golden Gate-based subdomain swap strategy

The pBAD plasmid containing the gene encoding Nl ACVS was virtually divided in three fragments, called A1, A2 and A3, designed in silico in such a way to exclude the Nl ACVS M1 native subdomain (S2 Fig). The three fragments were then individually amplified via PCR using KAPA HiFi HotStart ReadyMix (Roche), according to the manufacturer’s protocol, and sub-cloned into intermediate pMAL-c5x-BsrDI free vectors (mutated in house to remove the recognition site BsrDI, type IIs restriction enzyme used in the Golden Gate assembly). The DNA fragments encoding the selected subdomains were sub-cloned in the same intermediate vector. The constructs were then checked via restriction analysis and sequencing (Macrogen Inc.). Once all the parts were confirmed correct, Golden Gate assembly reactions were performed to build the hybrid NRPSs. For this, the intermediate vectors pMAL-c5x-A1, pMAL-c5x-A2, pMAL-c5x-A3 and pMAL-c5x-sub_x were mixed with a molar ratio of 1:1:0.5:1, with a total amount of DNA ~ 500 ng. Subsequently, 1 μL of T4 DNA Ligase (5 U/μL), 1 μL of T4 DNA Ligase 10x buffer and 0.5 μL of BsrDI (5 U/μL) (Invitrogen, Thermo Fisher Scientific) were added to complete the reaction mixture in a total volume of 10 μL. The Golden Gate assembly reactions were carried out in a C1000 Thermal Cycler (Bio-Rad), with the standard 50x cycles-protocol [34]. The reaction mixtures were directly transformed into chemically-competent E. coli DH5α cells. The plasmids were subsequently mini-prepped and checked as described previously.

Expression and His-tag affinity purification of Nl ACVS and hybrid NRPSs

Cultures were grown to an OD₆₀₀ of 0.6, transferred to 18°C and 200 rpm for 1h and subsequently induced using 0.2% L-arabinose. Harvest followed 18h after induction by spinning at 4000 g for 15 minutes. After resuspension in lysis buffer (HEPES 50 mM pH 7.0, 300 mM NaCl, 2 mM DTT, Complete EDTA free protease inhibitor; Roche No. 04693159001), cells were disrupted using sonication (6s/15s on/off; 50x; 10μm amplitude; Soniprep 150 MSE) and cell-free lysate obtained by centrifugation at 4°C, 17000g, 15 minutes. Purified enzyme was extracted by means of Ni²⁺ affinity purification using gravity flow. Wash steps were performed using two column volumes of wash buffer (HEPES 50 mM pH 7.0, 300 mM NaCl, 20 mM imidazole) followed by a three-step elution using one bed volume of each elution buffer (HEPES 50 mM pH7.0, NaCl 300 mM, imidazole 50–150 or 250 mM). Samples were concentrated if necessary, using Amicon U-100 spin filters (Merck). Final concentration was determined using A₂₈₀ (NanoDrop 1000; Thermo Fisher Scientific).

In vitro product formation assay

Isolated enzymes (fraction E3) were subjected to in vitro assays, in order to determine product formation properties. Assay conditions initially used include HEPES 50 mM pH 7.0, 300 mM NaCl, 5 mM ATP pH 7.0, 100 μM CoA, 0.2 μM phosphopantetheinyl transferase (Sfp, NEB), 5 mM L-α-aminoadipic acid, 2 mM L-cysteine, 2 mM L-valine (5 mM L-Val for the hybrid VCVS), 5 mM MgCl₂, 2 mM DTT and 0.17 μM ACVS, or 0.5–1 μM for the hybrids. For velocity and affinity determination, amino acid concentration of 0.1, 0.25, 0.5, 1, 2 and 5 mM were used. For the hybrids’ reactions and promiscuity determination the concentration of the variable amino acid was set at 5mM. Reactions were run at 30°C and sampling took place after 0, 10, 20, 30, 45, 60, 120 and 240 minutes for dynamic measurements and after 0 and 240 or 960 minutes for endpoint value determination. NaOH was added to each sample to a final concentration of 0.1 M to quench the reactions. Samples were subsequently stored at -80°C and reduced before HPLC/MS analysis adding DTT to a concentration of 5 mM.

High performance liquid chromatographic and mass-spectrometric analysis (HPLC/MS)

Samples (50 μL) obtained from an in vitro reaction were subjected to HPLC/MS analysis. Two technical replicates were run per sample at 5 μL each. Analysis was performed using a LC/MS Orbitrap (Thermo Scientific) in combination with a RP-C18 column (Shimadzu Shim pack XR-ODS 2.2; 3.0x75mm). Scan range was set at 80–1600 M/Z in positive ion (4.2kV spray, 87.5V capillary and 120V of tube lens) mode, with capillary temperature set at 325°C. A gradient program with MilliQ water (A), Acetonitrile (B) and 2% Formic acid (C) was run: 0 min, A 90%, B 5%, C 5%; 4 min, A 90%, B 5%, C 5%; 13 min, A 0%, B 95%, C 5%; 16 min, A 0%, B 95%, C 5%; 16 min, A 90%, B 5%, C 5%; 21 min, A 90%, B 5%, C 5% at a flow rate of 0.3 ml min^-1. The Bis-ACV standard was obtained from Bachem, reduced to (L,L,D)-ACV and used for quantification in a standard curve at concentrations of 0.1, 0.5, 1, 5, 10, 50 and 100 μM. Alternative tripeptides were identified according to accurate monoisotopic mass, if not mentioned otherwise.

Supporting information

S1 Fig. Selection criteria for donor subdomain templates.

The donor subdomains were selected according to three criteria. First, we individually aligned the full donor A domains to the ACVS M1 A domain (L-Aaa) and determined the sequence identity (we selected those with identity higher than 30% for further analysis, in green). We then determined the size of the subdomain, using as boundaries the regions described in the methods section and Fig 5; those with a similar size to the wild-type subdomain were aligned with the latter, to determine the identity between the subdomains themselves. The ones with highest identity were selected and designed in silico for the assembly strategy (highlighted in dark green). Targets with A domains sequence identities below 30% were not further included in the analyses; *local misalignments that prevented the determination of the subdomains boundaries.

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S2 Fig. Golden gate-based subdomain swap strategy.

(A) pBAD-Nl ACVS His-tag plasmid map (exported from Snapgene). (B) Hybrid NRPSs assembly strategy: three fragments (named A1, A2 and A3) were amplified via PCR from the plasmid in such a way to amplify the gene together with the vector and exclude the subdomain of module 1; the three fragments were cloned into pMAL-c5x-BsrDI free intermediate vectors (BsrDI sites are presents at the ends of the A1, A2 and A3 fragments for the Golden Gate assembly); the synthetic donor subdomains (Asub_x) are also cloned into the same intermediate vector. A1, A2, A3 and Asub_x have complementary overhangs (indicated by numbers 1–4) after digestion with BsrDI, allowing the Golden Gate assembly reaction.

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S3 Fig. Predicted structures of hybrid tripeptides.

The structures (wild-type product ACV on top) were drawn using MarvinSketch (ChemAxon), and exact molecular weights were determined using the ‘Elemental analysis’ tool of the same software.

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S1 Appendix. LC chromatograms and mass spectra of characterized tripeptides.

The full chromatograms were filtered in accordance with the predicted m/z value of each tripeptide. The mass spectra of the resulting peaks were scanned for the presence of the expected compound.

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S1 Raw Images.

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Acknowledgments

We thank DSM Sinochem Pharmaceuticals (now Centrient Pharmaceuticals) for the Nl ACVS construct, and in particular Jan-Metske van der Laan for the scientific support. We also would like to thank Susan Fekken for her help with the kinetics experiments.

References

1. Von Döhren H, Dieckmann R, Pavela-Vrancic M. The nonribosomal code. Chem Biol. 1999;6(10):273–9.
- View Article
- Google Scholar
2. Marahiel M a., Essen LO. Chapter 13 Nonribosomal Peptide Synthetases. Mechanistic and Structural Aspects of Essential Domains [Internet]. 1st ed. Vol. 458, Methods in Enzymology. Elsevier Inc.; 2009. 337–351 p. Available from: https://doi.org/10.1016/S0076-6879(09)04813-7 pmid:19374989
3. Strieker M, Tanović A, Marahiel M a. Nonribosomal peptide synthetases: Structures and dynamics. Curr Opin Struct Biol. 2010;20(2):234–40. pmid:20153164
- View Article
- PubMed/NCBI
- Google Scholar
4. Condurso HL, Bruner SD. Structure and noncanonical chemistry of nonribosomal peptide biosynthetic machinery. [Internet]. Vol. 29, Natural product reports. 2012. p. 1099–110. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22729219%5Cnhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC3442147%5Cnhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3442147&tool=pmcentrez&rendertype=abstract%5Cnhttp://www.ncbi.nlm.nih.gov/pubm pmid:22729219
- View Article
- PubMed/NCBI
- Google Scholar
5. Koglin A, Walsh CT. Structural insights into nonribosomal peptide enzymatic assembly lines. Nat Prod Rep. 2009;26(8):987–1000. pmid:19636447
- View Article
- PubMed/NCBI
- Google Scholar
6. Walsh CT. Insights into the chemical logic and enzymatic machinery of NRPS assembly lines. Nat Prod Rep [Internet]. 2016;33(2):127–35. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26175103%5Cnhttp://xlink.rsc.org/?DOI=C5NP00035A pmid:26175103
- View Article
- PubMed/NCBI
- Google Scholar
7. Fischbach M a., Walsh CT. Assembly-line enzymology for polyketide and nonribosomal peptide antibiotics: Logic machinery, and mechanisms. Chem Rev. 2006;106(8):3468–96. pmid:16895337
- View Article
- PubMed/NCBI
- Google Scholar
8. Kohli RM, Walsh CT. Enzymology of acyl chain macrocyclization in natural product biosynthesis. Chem Commun. 2003;3(3):297–307.
- View Article
- Google Scholar
9. Schneider A, Marahiel MA. Genetic evidence for a role of thioesterase domains, integrated in or associated with peptide synthetases, in non-ribosomal peptide biosynthesis in Bacillus subtilis. Arch Microbiol. 1998;169(5):404–10. pmid:9560421
- View Article
- PubMed/NCBI
- Google Scholar
10. Keating T a Marshall CG, Walsh CT Keating AE. The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains. Nat Struct Biol. 2002;9(7):522–6. pmid:12055621
- View Article
- PubMed/NCBI
- Google Scholar
11. Samel SA, Schoenafinger G, Knappe TA, Marahiel MA, Essen LO. Structural and Functional Insights into a Peptide Bond-Forming Bidomain from a Nonribosomal Peptide Synthetase. Structure. 2007;15(7):781–92. pmid:17637339
- View Article
- PubMed/NCBI
- Google Scholar
12. Tanovic A, Samel SA, Essen L-O, Marahiel MA. Crystal structure of the termination module of a nonribosomal peptide synthetase. Science [Internet]. 2008;321(5889):659–63. Available from: http://www.sciencemag.org/cgi/doi/10.1126/science.1159850%5Cnhttp://www.ncbi.nlm.nih.gov/pubmed/18583577 pmid:18583577
- View Article
- PubMed/NCBI
- Google Scholar
13. Tan XF, Dai YN, Zhou K, Jiang YL, Ren YM, Chen Y, et al. Structure of the adenylation-peptidyl carrier protein didomain of the Microcystis aeruginosa microcystin synthetase McyG. Acta Crystallogr D Biol Crystallogr [Internet]. 2015;71(Pt 4):873–81. Available from: http://apps.webofknowledge.com.kuleuven.ezproxy.kuleuven.be/full_record.do?product=UA&search_mode=GeneralSearch&qid=13&SID=T2TkRQsVroWppTl4jjS&page=1&doc=1 pmid:25849398
- View Article
- PubMed/NCBI
- Google Scholar
14. Drake EJ, Miller BR, Shi C, Tarrasch JT, Sundlov JA, Allen CL, et al. Structures of two distinct conformations of holo-non-ribosomal peptide synthetases. Nature [Internet]. 2016;529(7585):235–8. Available from: http://dx.doi.org/10.1038/nature16163 pmid:26762461
- View Article
- PubMed/NCBI
- Google Scholar
15. Tarry MJ, Haque AS, Bui KH, Schmeing TM. X-Ray Crystallography and Electron Microscopy of Cross- and Multi-Module Nonribosomal Peptide Synthetase Proteins Reveal a Flexible Architecture. Structure [Internet]. 2017;1–11. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0969212617300746
- View Article
- Google Scholar
16. Reimer JM, Eivaskhani M, Harb I, Guarné A, Weigt M, Martin Schmeing T. Structures of a dimodular nonribosomal peptide synthetase reveal conformational flexibility. Science (80-). 2019;366(6466).
- View Article
- Google Scholar
17. Baldwin JE, Abraham E. The biosynthesis of penicillins and cephalosporins. Nat Prod Rep [Internet]. 1988;5(2):129. Available from: pmid:3145474
- View Article
- PubMed/NCBI
- Google Scholar
18. Roach PL, Clifton IJ, Hensgens CM, Shibata N, Schofield CJ, Hajdu J, et al. Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation. Nature. 1997;387(1991):827–30.
- View Article
- Google Scholar
19. Martín JF. New aspects of genes and enzymes for β-lactam antibiotic biosynthesis. Appl Microbiol Biotechnol. 1998;50(1):1–15. pmid:9720195
- View Article
- PubMed/NCBI
- Google Scholar
20. Liras P. Biosynthesis and molecular genetics of cephamycins. Antonie van Leeuwenhoek, Int J Gen Mol Microbiol. 1999;75(1–2):109–24.
- View Article
- Google Scholar
21. Brakhage AA. Molecular regulation of penicillin biosynthesis in Aspergillus (Emericella) nidulans. FEMS Microbiol Lett. 1997;148(1):1–10. pmid:9066103
- View Article
- PubMed/NCBI
- Google Scholar
22. Ozcengiz G, Demain AL. Recent advances in the biosynthesis of penicillins, cephalosporins and clavams and its regulation. Biotechnol Adv [Internet]. 2013;31(2):287–311. Available from: pmid:23228980
- View Article
- PubMed/NCBI
- Google Scholar
23. Marahiel M a, Stachelhaus T, Mootz HD. Modular Peptide Synthetases Involved in Nonribosomal Peptide Synthesis. Chem Rev [Internet]. 1997;97(7):2651–74. Available from: http://www.ncbi.nlm.nih.gov/pubmed/11851476 pmid:11851476
- View Article
- PubMed/NCBI
- Google Scholar
24. Baldwin JE, Bird JW, Field RA, O’Callaghan NM, Schofield CJ. Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus. Vol. 43, The Journal of antibiotics. Japan; 1990. p. 1055–7.
- View Article
- Google Scholar
25. Baldwin JE, Bird JW, Field RA, O’Callaghan NM, Schofield CJ, Willis AC. Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus. Evidence for the presence of phosphopantothenate in ACV synthetase. J Antibiot (Tokyo). 1991 Feb;44(2):241–8.
- View Article
- Google Scholar
26. Jensen SE, Wong A, Rollins MJ, Westlake DW. Purification and partial characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Streptomyces clavuligerus. J Bacteriol. 1990 Dec;172(12):7269–71. pmid:2254285
- View Article
- PubMed/NCBI
- Google Scholar
27. Theilgaard HB, Kristiansen KN, Henriksen CM, Nielsen J. Purification and characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Penicillium chrysogenum. Biochem J. 1997 Oct;327 (Pt 1):185–91.
- View Article
- Google Scholar
28. van Liempt H, von Dohren H, Kleinkauf H. delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Aspergillus nidulans. The first enzyme in penicillin biosynthesis is a multifunctional peptide synthetase. J Biol Chem. 1989 Mar;264(7):3680–4. pmid:2645274
- View Article
- PubMed/NCBI
- Google Scholar
29. Coque JJ, Martin JF, Calzada JG, Liras P. The cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nocardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum. Mol Microbiol. 1991 May;5(5):1125–33. pmid:1956290
- View Article
- PubMed/NCBI
- Google Scholar
30. Tahlan K, Moore MA, Jensen SE. $δ$-(l-$α$-aminoadipyl)-l-cysteinyl-d-valine synthetase (ACVS): discovery and perspectives. J Ind Microbiol {&} Biotechnol. 2017;44(4):517–24.
- View Article
- Google Scholar
31. Coque JJR, De La Fuente JL, Liras P, Martín JF. Overexpression of the Nocardia lactamdurans α-aminoadipyl-cysteinyl-valine synthetase in Streptomyces lividans. The purified multienzyme uses cystathionine and 6-oxopiperidine 2-carboxylate as substrates for synthesis of the tripeptide. Eur J Biochem. 1996;242(2):264–70. pmid:8973642
- View Article
- PubMed/NCBI
- Google Scholar
32. Gruenewald S, Mootz HD, Stehmeier P, Stachelhaus T. In vivo production of artificial nonribosomal peptide products in the heterologous host Escherichia coli. Appl Environ Microbiol. 2004;70(6):3282–91. pmid:15184122
- View Article
- PubMed/NCBI
- Google Scholar
33. Kries H, Niquille DL, Hilvert D. A Subdomain Swap Strategy for Reengineering Nonribosomal Peptides. Chem Biol [Internet]. 2015;22(5):640–8. Available from: pmid:26000750
- View Article
- PubMed/NCBI
- Google Scholar
34. Engler C, Gruetzner R, Kandzia R, Marillonnet S. Golden gate shuffling: A one-pot DNA shuffling method based on type ils restriction enzymes. PLoS One. 2009;4(5).
- View Article
- Google Scholar
35. Stachelhaus T, D. Mootz H, Marahiel MA. The specificity-conferring code of adenylation domains in nonribosomal peptide synthetases. Chem Biol. 1999;6(8):493–505. pmid:10421756
- View Article
- PubMed/NCBI
- Google Scholar
36. Sun X, Li H, Alfermann J, Mootz HD, Yang H. Kinetics Profiling of Gramicidin S Synthetase A, a Member of Nonribosomal Peptide Synthetases. Biochemistry [Internet]. 2014 Dec 23;53(50):7983–9. Available from: pmid:25437123
- View Article
- PubMed/NCBI
- Google Scholar
37. Schwecke T, Aharonowitz Y, Palissa H, von Dohren H, Kleinkauf H, van Liempt H. Enzymatic characterisation of the multifunctional enzyme delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Streptomyces clavuligerus. Eur J Biochem. 1992 Apr;205(2):687–94. pmid:1572368
- View Article
- PubMed/NCBI
- Google Scholar
38. Zhang J, Wolfe S, Demain AL. Biochemical studies on the activity of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Streptomyces clavuligerus. Biochem J. 1992 May;283 (Pt 3):691–8.
- View Article
- Google Scholar
39. Etchegaray A, Dieckmann R, Kennedy J, Turner G, von Döhren H. ACV synthetase: expression of amino acid activating domains of the Penicillium chrysogenum enzyme in Aspergillus nidulans. Biochem Biophys Res Commun. 1997;237(1):166–9. pmid:9266851
- View Article
- PubMed/NCBI
- Google Scholar
40. Baldwin JE, Shiau CY, Byford MF, Schofield CJ. Substrate specificity of L-delta-(alpha-aminoadipoyl)-L-cysteinyl-D-valine synthetase from Cephalosporium acremonium: demonstration of the structure of several unnatural tripeptide products. Biochem J [Internet]. 1994;301 (Pt 2)(1 994):367–72. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1137089&tool=pmcentrez&rendertype=abstract
- View Article
- Google Scholar
41. Conti E, Stachelhaus T, Marahiel MA, Brick P. Structural basis for the activation of phenylalanine in the non-ribosomal biosynthesis of gramicidin S. 1997;16(14):4174–83. pmid:9250661
- View Article
- PubMed/NCBI
- Google Scholar
42. Nguyen KT, Ritz D, Gu J-Q, Alexander D, Chu M, Miao V, et al. Combinatorial biosynthesis of novel antibiotics related to daptomycin. Proc Natl Acad Sci U S A [Internet]. 2006;103(46):17462–7. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1859951&tool=pmcentrez&rendertype=abstract pmid:17090667
- View Article
- PubMed/NCBI
- Google Scholar
43. Miao V, Coëffet-Le Gal MF, Nguyen K, Brian P, Penn J, Whiting A, et al. Genetic Engineering in Streptomyces roseosporus to Produce Hybrid Lipopeptide Antibiotics. Chem Biol. 2006;13(3):269–76. pmid:16638532
- View Article
- PubMed/NCBI
- Google Scholar
44. Yu D, Xu F, Gage D, Zhan J. Functional dissection and module swapping of fungal cyclooligomer depsipeptide synthetases. Chem Commun. 2013;49(55):6176–8.
- View Article
- Google Scholar
45. Crüsemann M, Kohlhaas C, Piel J. Evolution-guided engineering of nonribosomal peptide synthetase adenylation domains. Chem Sci [Internet]. 2013;4(3):1041. Available from: http://xlink.rsc.org/?DOI=c2sc21722h
- View Article
- Google Scholar
46. Bian X, Plaza A, Yan F, Zhang Y, Müller R. Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide derivatives in vivo. Biotechnol Bioeng. 2015;112(7):1343–53. pmid:25683597
- View Article
- PubMed/NCBI
- Google Scholar
47. Winn M, Fyans JK, Zhuo Y, Micklefield J. Recent advances in engineering nonribosomal peptide assembly lines. Nat Prod Rep [Internet]. 2016; Available from: http://xlink.rsc.org/?DOI=C5NP00099H
- View Article
- Google Scholar
48. Belshaw PJ, Walsh CT, Stachelhaus T. Aminoacyl-CoAs as probes of condensation domain selectivity in nonribosomal peptide synthesis. Science. 1999;284(5413):486–9. pmid:10205056
- View Article
- PubMed/NCBI
- Google Scholar
49. Bloudoff K, Alonzo DA, Schmeing TM, Bloudoff K, Alonzo DA, Schmeing TM. Chemical Probes Allow Structural Insight into the Condensation Reaction of Nonribosomal Peptide Chemical Probes Allow Structural Insight into the Condensation Reaction of Nonribosomal Peptide Synthetases. Cell Chem Biol [Internet]. 2016;23(3):331–9. Available from: pmid:26991102
- View Article
- PubMed/NCBI
- Google Scholar
50. Caboche S, Pupin M, Leclère V, Fontaine A, Jacques P, Kucherov G. NORINE: A database of nonribosomal peptides. Nucleic Acids Res. 2008;36(SUPPL. 1):326–31.
- View Article
- Google Scholar
51. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016;33(7):1870–4. pmid:27004904
- View Article
- PubMed/NCBI
- Google Scholar
52. Okonechnikov K, Golosova O, Fursov M, Varlamov A, Vaskin Y, Efremov I, et al. Unipro UGENE: A unified bioinformatics toolkit. Bioinformatics. 2012;28(8):1166–7. pmid:22368248
- View Article
- PubMed/NCBI
- Google Scholar

[ref1] 1. Von Döhren H, Dieckmann R, Pavela-Vrancic M. The nonribosomal code. Chem Biol. 1999;6(10):273–9.
View Article
Google Scholar

[2] View Article

[3] Google Scholar

[ref2] 2. Marahiel M a., Essen LO. Chapter 13 Nonribosomal Peptide Synthetases. Mechanistic and Structural Aspects of Essential Domains [Internet]. 1st ed. Vol. 458, Methods in Enzymology. Elsevier Inc.; 2009. 337–351 p. Available from: https://doi.org/10.1016/S0076-6879(09)04813-7 pmid:19374989

[ref3] 3. Strieker M, Tanović A, Marahiel M a. Nonribosomal peptide synthetases: Structures and dynamics. Curr Opin Struct Biol. 2010;20(2):234–40. pmid:20153164
View Article
PubMed/NCBI
Google Scholar

[6] View Article

[7] PubMed/NCBI

[8] Google Scholar

[ref4] 4. Condurso HL, Bruner SD. Structure and noncanonical chemistry of nonribosomal peptide biosynthetic machinery. [Internet]. Vol. 29, Natural product reports. 2012. p. 1099–110. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22729219%5Cnhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC3442147%5Cn http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3442147&tool=pmcentrez&rendertype=abstract%5Cn http://www.ncbi.nlm.nih.gov/pubm pmid:22729219
View Article
PubMed/NCBI
Google Scholar

[10] View Article

[11] PubMed/NCBI

[12] Google Scholar

[ref5] 5. Koglin A, Walsh CT. Structural insights into nonribosomal peptide enzymatic assembly lines. Nat Prod Rep. 2009;26(8):987–1000. pmid:19636447
View Article
PubMed/NCBI
Google Scholar

[14] View Article

[15] PubMed/NCBI

[16] Google Scholar

[ref6] 6. Walsh CT. Insights into the chemical logic and enzymatic machinery of NRPS assembly lines. Nat Prod Rep [Internet]. 2016;33(2):127–35. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26175103%5Cnhttp://xlink.rsc.org/?DOI=C5NP00035A pmid:26175103
View Article
PubMed/NCBI
Google Scholar

[18] View Article

[19] PubMed/NCBI

[20] Google Scholar

[ref7] 7. Fischbach M a., Walsh CT. Assembly-line enzymology for polyketide and nonribosomal peptide antibiotics: Logic machinery, and mechanisms. Chem Rev. 2006;106(8):3468–96. pmid:16895337
View Article
PubMed/NCBI
Google Scholar

[22] View Article

[23] PubMed/NCBI

[24] Google Scholar

[ref8] 8. Kohli RM, Walsh CT. Enzymology of acyl chain macrocyclization in natural product biosynthesis. Chem Commun. 2003;3(3):297–307.
View Article
Google Scholar

[26] View Article

[27] Google Scholar

[ref9] 9. Schneider A, Marahiel MA. Genetic evidence for a role of thioesterase domains, integrated in or associated with peptide synthetases, in non-ribosomal peptide biosynthesis in Bacillus subtilis. Arch Microbiol. 1998;169(5):404–10. pmid:9560421
View Article
PubMed/NCBI
Google Scholar

[29] View Article

[30] PubMed/NCBI

[31] Google Scholar

[ref10] 10. Keating T a Marshall CG, Walsh CT Keating AE. The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains. Nat Struct Biol. 2002;9(7):522–6. pmid:12055621
View Article
PubMed/NCBI
Google Scholar

[33] View Article

[34] PubMed/NCBI

[35] Google Scholar

[ref11] 11. Samel SA, Schoenafinger G, Knappe TA, Marahiel MA, Essen LO. Structural and Functional Insights into a Peptide Bond-Forming Bidomain from a Nonribosomal Peptide Synthetase. Structure. 2007;15(7):781–92. pmid:17637339
View Article
PubMed/NCBI
Google Scholar

[37] View Article

[38] PubMed/NCBI

[39] Google Scholar

[ref12] 12. Tanovic A, Samel SA, Essen L-O, Marahiel MA. Crystal structure of the termination module of a nonribosomal peptide synthetase. Science [Internet]. 2008;321(5889):659–63. Available from: http://www.sciencemag.org/cgi/doi/10.1126/science.1159850%5Cnhttp://www.ncbi.nlm.nih.gov/pubmed/18583577 pmid:18583577
View Article
PubMed/NCBI
Google Scholar

[41] View Article

[42] PubMed/NCBI

[43] Google Scholar

[ref13] 13. Tan XF, Dai YN, Zhou K, Jiang YL, Ren YM, Chen Y, et al. Structure of the adenylation-peptidyl carrier protein didomain of the Microcystis aeruginosa microcystin synthetase McyG. Acta Crystallogr D Biol Crystallogr [Internet]. 2015;71(Pt 4):873–81. Available from: http://apps.webofknowledge.com.kuleuven.ezproxy.kuleuven.be/full_record.do?product=UA&search_mode=GeneralSearch&qid=13&SID=T2TkRQsVroWppTl4jjS&page=1&doc=1 pmid:25849398
View Article
PubMed/NCBI
Google Scholar

[45] View Article

[46] PubMed/NCBI

[47] Google Scholar

[ref14] 14. Drake EJ, Miller BR, Shi C, Tarrasch JT, Sundlov JA, Allen CL, et al. Structures of two distinct conformations of holo-non-ribosomal peptide synthetases. Nature [Internet]. 2016;529(7585):235–8. Available from: http://dx.doi.org/10.1038/nature16163 pmid:26762461
View Article
PubMed/NCBI
Google Scholar

[49] View Article

[50] PubMed/NCBI

[51] Google Scholar

[ref15] 15. Tarry MJ, Haque AS, Bui KH, Schmeing TM. X-Ray Crystallography and Electron Microscopy of Cross- and Multi-Module Nonribosomal Peptide Synthetase Proteins Reveal a Flexible Architecture. Structure [Internet]. 2017;1–11. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0969212617300746
View Article
Google Scholar

[53] View Article

[54] Google Scholar

[ref16] 16. Reimer JM, Eivaskhani M, Harb I, Guarné A, Weigt M, Martin Schmeing T. Structures of a dimodular nonribosomal peptide synthetase reveal conformational flexibility. Science (80-). 2019;366(6466).
View Article
Google Scholar

[56] View Article

[57] Google Scholar

[ref17] 17. Baldwin JE, Abraham E. The biosynthesis of penicillins and cephalosporins. Nat Prod Rep [Internet]. 1988;5(2):129. Available from: pmid:3145474
View Article
PubMed/NCBI
Google Scholar

[59] View Article

[60] PubMed/NCBI

[61] Google Scholar

[ref18] 18. Roach PL, Clifton IJ, Hensgens CM, Shibata N, Schofield CJ, Hajdu J, et al. Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation. Nature. 1997;387(1991):827–30.
View Article
Google Scholar

[63] View Article

[64] Google Scholar

[ref19] 19. Martín JF. New aspects of genes and enzymes for β-lactam antibiotic biosynthesis. Appl Microbiol Biotechnol. 1998;50(1):1–15. pmid:9720195
View Article
PubMed/NCBI
Google Scholar

[66] View Article

[67] PubMed/NCBI

[68] Google Scholar

[ref20] 20. Liras P. Biosynthesis and molecular genetics of cephamycins. Antonie van Leeuwenhoek, Int J Gen Mol Microbiol. 1999;75(1–2):109–24.
View Article
Google Scholar

[70] View Article

[71] Google Scholar

[ref21] 21. Brakhage AA. Molecular regulation of penicillin biosynthesis in Aspergillus (Emericella) nidulans. FEMS Microbiol Lett. 1997;148(1):1–10. pmid:9066103
View Article
PubMed/NCBI
Google Scholar

[73] View Article

[74] PubMed/NCBI

[75] Google Scholar

[ref22] 22. Ozcengiz G, Demain AL. Recent advances in the biosynthesis of penicillins, cephalosporins and clavams and its regulation. Biotechnol Adv [Internet]. 2013;31(2):287–311. Available from: pmid:23228980
View Article
PubMed/NCBI
Google Scholar

[77] View Article

[78] PubMed/NCBI

[79] Google Scholar

[ref23] 23. Marahiel M a, Stachelhaus T, Mootz HD. Modular Peptide Synthetases Involved in Nonribosomal Peptide Synthesis. Chem Rev [Internet]. 1997;97(7):2651–74. Available from: http://www.ncbi.nlm.nih.gov/pubmed/11851476 pmid:11851476
View Article
PubMed/NCBI
Google Scholar

[81] View Article

[82] PubMed/NCBI

[83] Google Scholar

[ref24] 24. Baldwin JE, Bird JW, Field RA, O’Callaghan NM, Schofield CJ. Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus. Vol. 43, The Journal of antibiotics. Japan; 1990. p. 1055–7.
View Article
Google Scholar

[85] View Article

[86] Google Scholar

[ref25] 25. Baldwin JE, Bird JW, Field RA, O’Callaghan NM, Schofield CJ, Willis AC. Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus. Evidence for the presence of phosphopantothenate in ACV synthetase. J Antibiot (Tokyo). 1991 Feb;44(2):241–8.
View Article
Google Scholar

[88] View Article

[89] Google Scholar

[ref26] 26. Jensen SE, Wong A, Rollins MJ, Westlake DW. Purification and partial characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Streptomyces clavuligerus. J Bacteriol. 1990 Dec;172(12):7269–71. pmid:2254285
View Article
PubMed/NCBI
Google Scholar

[91] View Article

[92] PubMed/NCBI

[93] Google Scholar

[ref27] 27. Theilgaard HB, Kristiansen KN, Henriksen CM, Nielsen J. Purification and characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Penicillium chrysogenum. Biochem J. 1997 Oct;327 (Pt 1):185–91.
View Article
Google Scholar

[95] View Article

[96] Google Scholar

[ref28] 28. van Liempt H, von Dohren H, Kleinkauf H. delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Aspergillus nidulans. The first enzyme in penicillin biosynthesis is a multifunctional peptide synthetase. J Biol Chem. 1989 Mar;264(7):3680–4. pmid:2645274
View Article
PubMed/NCBI
Google Scholar

[98] View Article

[99] PubMed/NCBI

[100] Google Scholar

[ref29] 29. Coque JJ, Martin JF, Calzada JG, Liras P. The cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nocardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum. Mol Microbiol. 1991 May;5(5):1125–33. pmid:1956290
View Article
PubMed/NCBI
Google Scholar

[102] View Article

[103] PubMed/NCBI

[104] Google Scholar

[ref30] 30. Tahlan K, Moore MA, Jensen SE. $δ$-(l-$α$-aminoadipyl)-l-cysteinyl-d-valine synthetase (ACVS): discovery and perspectives. J Ind Microbiol {&} Biotechnol. 2017;44(4):517–24.
View Article
Google Scholar

[106] View Article

[107] Google Scholar

[ref31] 31. Coque JJR, De La Fuente JL, Liras P, Martín JF. Overexpression of the Nocardia lactamdurans α-aminoadipyl-cysteinyl-valine synthetase in Streptomyces lividans. The purified multienzyme uses cystathionine and 6-oxopiperidine 2-carboxylate as substrates for synthesis of the tripeptide. Eur J Biochem. 1996;242(2):264–70. pmid:8973642
View Article
PubMed/NCBI
Google Scholar

[109] View Article

[110] PubMed/NCBI

[111] Google Scholar

[ref32] 32. Gruenewald S, Mootz HD, Stehmeier P, Stachelhaus T. In vivo production of artificial nonribosomal peptide products in the heterologous host Escherichia coli. Appl Environ Microbiol. 2004;70(6):3282–91. pmid:15184122
View Article
PubMed/NCBI
Google Scholar

[113] View Article

[114] PubMed/NCBI

[115] Google Scholar

[ref33] 33. Kries H, Niquille DL, Hilvert D. A Subdomain Swap Strategy for Reengineering Nonribosomal Peptides. Chem Biol [Internet]. 2015;22(5):640–8. Available from: pmid:26000750
View Article
PubMed/NCBI
Google Scholar

[117] View Article

[118] PubMed/NCBI

[119] Google Scholar

[ref34] 34. Engler C, Gruetzner R, Kandzia R, Marillonnet S. Golden gate shuffling: A one-pot DNA shuffling method based on type ils restriction enzymes. PLoS One. 2009;4(5).
View Article
Google Scholar

[121] View Article

[122] Google Scholar

[ref35] 35. Stachelhaus T, D. Mootz H, Marahiel MA. The specificity-conferring code of adenylation domains in nonribosomal peptide synthetases. Chem Biol. 1999;6(8):493–505. pmid:10421756
View Article
PubMed/NCBI
Google Scholar

[124] View Article

[125] PubMed/NCBI

[126] Google Scholar

[ref36] 36. Sun X, Li H, Alfermann J, Mootz HD, Yang H. Kinetics Profiling of Gramicidin S Synthetase A, a Member of Nonribosomal Peptide Synthetases. Biochemistry [Internet]. 2014 Dec 23;53(50):7983–9. Available from: pmid:25437123
View Article
PubMed/NCBI
Google Scholar

[128] View Article

[129] PubMed/NCBI

[130] Google Scholar

[ref37] 37. Schwecke T, Aharonowitz Y, Palissa H, von Dohren H, Kleinkauf H, van Liempt H. Enzymatic characterisation of the multifunctional enzyme delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Streptomyces clavuligerus. Eur J Biochem. 1992 Apr;205(2):687–94. pmid:1572368
View Article
PubMed/NCBI
Google Scholar

[132] View Article

[133] PubMed/NCBI

[134] Google Scholar

[ref38] 38. Zhang J, Wolfe S, Demain AL. Biochemical studies on the activity of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Streptomyces clavuligerus. Biochem J. 1992 May;283 (Pt 3):691–8.
View Article
Google Scholar

[136] View Article

[137] Google Scholar

[ref39] 39. Etchegaray A, Dieckmann R, Kennedy J, Turner G, von Döhren H. ACV synthetase: expression of amino acid activating domains of the Penicillium chrysogenum enzyme in Aspergillus nidulans. Biochem Biophys Res Commun. 1997;237(1):166–9. pmid:9266851
View Article
PubMed/NCBI
Google Scholar

[139] View Article

[140] PubMed/NCBI

[141] Google Scholar

[ref40] 40. Baldwin JE, Shiau CY, Byford MF, Schofield CJ. Substrate specificity of L-delta-(alpha-aminoadipoyl)-L-cysteinyl-D-valine synthetase from Cephalosporium acremonium: demonstration of the structure of several unnatural tripeptide products. Biochem J [Internet]. 1994;301 (Pt 2)(1 994):367–72. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1137089&tool=pmcentrez&rendertype=abstract
View Article
Google Scholar

[143] View Article

[144] Google Scholar

[ref41] 41. Conti E, Stachelhaus T, Marahiel MA, Brick P. Structural basis for the activation of phenylalanine in the non-ribosomal biosynthesis of gramicidin S. 1997;16(14):4174–83. pmid:9250661
View Article
PubMed/NCBI
Google Scholar

[146] View Article

[147] PubMed/NCBI

[148] Google Scholar

[ref42] 42. Nguyen KT, Ritz D, Gu J-Q, Alexander D, Chu M, Miao V, et al. Combinatorial biosynthesis of novel antibiotics related to daptomycin. Proc Natl Acad Sci U S A [Internet]. 2006;103(46):17462–7. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1859951&tool=pmcentrez&rendertype=abstract pmid:17090667
View Article
PubMed/NCBI
Google Scholar

[150] View Article

[151] PubMed/NCBI

[152] Google Scholar

[ref43] 43. Miao V, Coëffet-Le Gal MF, Nguyen K, Brian P, Penn J, Whiting A, et al. Genetic Engineering in Streptomyces roseosporus to Produce Hybrid Lipopeptide Antibiotics. Chem Biol. 2006;13(3):269–76. pmid:16638532
View Article
PubMed/NCBI
Google Scholar

[154] View Article

[155] PubMed/NCBI

[156] Google Scholar

[ref44] 44. Yu D, Xu F, Gage D, Zhan J. Functional dissection and module swapping of fungal cyclooligomer depsipeptide synthetases. Chem Commun. 2013;49(55):6176–8.
View Article
Google Scholar

[158] View Article

[159] Google Scholar

[ref45] 45. Crüsemann M, Kohlhaas C, Piel J. Evolution-guided engineering of nonribosomal peptide synthetase adenylation domains. Chem Sci [Internet]. 2013;4(3):1041. Available from: http://xlink.rsc.org/?DOI=c2sc21722h
View Article
Google Scholar

[161] View Article

[162] Google Scholar

[ref46] 46. Bian X, Plaza A, Yan F, Zhang Y, Müller R. Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide derivatives in vivo. Biotechnol Bioeng. 2015;112(7):1343–53. pmid:25683597
View Article
PubMed/NCBI
Google Scholar

[164] View Article

[165] PubMed/NCBI

[166] Google Scholar

[ref47] 47. Winn M, Fyans JK, Zhuo Y, Micklefield J. Recent advances in engineering nonribosomal peptide assembly lines. Nat Prod Rep [Internet]. 2016; Available from: http://xlink.rsc.org/?DOI=C5NP00099H
View Article
Google Scholar

[168] View Article

[169] Google Scholar

[ref48] 48. Belshaw PJ, Walsh CT, Stachelhaus T. Aminoacyl-CoAs as probes of condensation domain selectivity in nonribosomal peptide synthesis. Science. 1999;284(5413):486–9. pmid:10205056
View Article
PubMed/NCBI
Google Scholar

[171] View Article

[172] PubMed/NCBI

[173] Google Scholar

[ref49] 49. Bloudoff K, Alonzo DA, Schmeing TM, Bloudoff K, Alonzo DA, Schmeing TM. Chemical Probes Allow Structural Insight into the Condensation Reaction of Nonribosomal Peptide Chemical Probes Allow Structural Insight into the Condensation Reaction of Nonribosomal Peptide Synthetases. Cell Chem Biol [Internet]. 2016;23(3):331–9. Available from: pmid:26991102
View Article
PubMed/NCBI
Google Scholar

[175] View Article

[176] PubMed/NCBI

[177] Google Scholar

[ref50] 50. Caboche S, Pupin M, Leclère V, Fontaine A, Jacques P, Kucherov G. NORINE: A database of nonribosomal peptides. Nucleic Acids Res. 2008;36(SUPPL. 1):326–31.
View Article
Google Scholar

[179] View Article

[180] Google Scholar

[ref51] 51. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016;33(7):1870–4. pmid:27004904
View Article
PubMed/NCBI
Google Scholar

[182] View Article

[183] PubMed/NCBI

[184] Google Scholar

[ref52] 52. Okonechnikov K, Golosova O, Fursov M, Varlamov A, Vaskin Y, Efremov I, et al. Unipro UGENE: A unified bioinformatics toolkit. Bioinformatics. 2012;28(8):1166–7. pmid:22368248
View Article
PubMed/NCBI
Google Scholar

[186] View Article

[187] PubMed/NCBI

[188] Google Scholar

Figures

Abstract

Introduction

Results

Purification and biochemical characterization of the N. lactamdurans ACVS

Substrate specificity of the Nl ACVS

Engineering of the first adenylation domain of Nl ACVS

Discussion

Materials and methods

Strains, plasmids and general culturing conditions

Identification of swapping partners and multi-alignment analysis

Engineering of the first adenylation domain of Nl ACVS via a Golden Gate-based subdomain swap strategy

Expression and His-tag affinity purification of Nl ACVS and hybrid NRPSs

In vitro product formation assay

High performance liquid chromatographic and mass-spectrometric analysis (HPLC/MS)

Supporting information

S1 Fig. Selection criteria for donor subdomain templates.

S2 Fig. Golden gate-based subdomain swap strategy.

S3 Fig. Predicted structures of hybrid tripeptides.

S1 Appendix. LC chromatograms and mass spectra of characterized tripeptides.

S1 Raw Images.

Acknowledgments

References