Figures
Abstract
The aim of this study was to select a candidate deep-sea amphipod species suitable for connectivity analyses in areas around cobalt-rich ferromanganese crusts (CRCs). We applied DNA barcoding based on the mitochondrial protein-coding gene, cytochrome c oxidase subunit I (COI), to specimens collected from the Xufu Guyot (the JA06 Seamount) off southeastern Minami-Torishima Island in the North Pacific, where CRCs are distributed. We used baited traps to collect 37 specimens. Comparison of COI sequences with public reference databases (GenBank, BOLD) showed that almost all of the specimens belonged to the superfamily Lysianassoidea, which is known to be ubiquitous in deep-sea areas. In a molecular taxonomic analysis of these sequences, we detected 11 clades. One of these clades (group 9) composed of 18 sequences and was identified by DNA barcoding as a putative species belonging to Abyssorchomene, which has been reported from the New Hebrides Trench in the South Pacific. We considered this species to be a candidate for connectivity analysis and analyzed its genome by restriction site–associated DNA sequencing. The results showed that the genetic variation in this species is adequate for analyzing connectivity patterns in CRC areas in the future.
Citation: Iguchi A, Nishijima M, Yoshioka Y, Miyagi A, Miwa R, Tanaka Y, et al. (2020) Deep-sea amphipods around cobalt-rich ferromanganese crusts: Taxonomic diversity and selection of candidate species for connectivity analysis. PLoS ONE 15(2): e0228483. https://doi.org/10.1371/journal.pone.0228483
Editor: Tzen-Yuh Chiang, National Cheng Kung University, TAIWAN
Received: May 30, 2019; Accepted: January 16, 2020; Published: February 6, 2020
Copyright: © 2020 Iguchi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All sequence data have been deposited in the DDBJ database (accession nos. LC484978- LC485014). Short-read data have been deposited in the DDBJ data base (accession nos. DRA008512 and DRA008890). All relevant data are within the manuscript and its Supporting Information files.
Funding: Kaiyo Engineering provided support in the form of salaries for authors MN and RM, and Japan Oil, Gas and Metals National Corporation (JOGMEC) provided support in the form of salaries for authors SK, TM, YI, and NO, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ʻauthor contributionsʼ section.
Competing interests: Kaiyo Engineering provided support in the form of salaries for authors MN and RM, and Japan Oil, Gas and Metals National Corporation (JOGMEC) provided support in the form of salaries for authors SK, TM, YI, and NO. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Introduction
Cobalt-rich ferromanganese crusts (CRCs) in deep-sea areas are attracting international attention as possible future sources of marine minerals. CRCs occur on the summits and slopes of seamounts (e.g., large flat-topped guyots), which are characterized by a unique fauna [1]. The International Seabed Authority (ISA) strongly recommends that contractors for exploration and exploitation of CRCs carry out baseline surveys for marine fauna and environmental conditions following ISA guidelines [2]. Specific requirements include information about biological communities. in particular is strongly required. The marine benthos around CRCs includes the animal communities that are most likely to be disturbed by the mining of marine minerals because benthic organisms live at or near the seafloor surface. Environmental impact assessments of the population dynamics and resilience of the deep-sea marine benthos around CRCs are thus essential before deep-sea mining can be initiated [1]. These assessments require evaluation of the genetic diversity and connectivity of the benthic populations. DNA markers for obtaining such information about populations have been broadly applied to many animal taxa [3], including the deep-sea benthos [4,5].
One difficulty in performing population-level studies in deep-sea areas is that many of the species are unknown or undescribed; few taxonomic studies are available because sampling sites are difficult to access [6]. In the former case, DNA barcoding is a promising approach for selecting putative species for connectivity analysis under these circumstances [7,8]. Whether DNA barcoding can be used to distinguish a putative species depends on the targeted DNA regions and the evolutionary history of the targeted taxon (e.g., the degree of lineage sorting). A fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) has been used as a standard “taxon barcode” for many animal groups [7,9], and this locus is also known to have relatively high resolution at inter- and intraspecies levels [8]. In addition, a huge number of COI sequences have been registered in public databases [9] and can be used to infer which species might be similar to an unknown or undescribed species.
Among deep-sea benthic taxa, the order Amphipoda (Crustacea: Malacostraca) is a major group and plays important roles in the deep-sea ecosystem. Furthermore, amphipods can be easily collected by using simple baited traps [10]. Thus, amphipod species in deep-sea areas might be good candidates for investigating connectivity patterns in deep-sea areas including those where CRCs are located. However, deep-sea amphipods include many cryptic species [10]. In this study, we applied DNA barcoding based on COI to deep-sea amphipods randomly collected from the JA06 seamount. CRCs are widely distributed around this seamount, which is situated near Minami-Torishima Island in the North Pacific [11], and the International Seabed Authority (ISA) has licensed the area for mineral exploration and exploitation. In addition, we performed a restriction site–associated DNA (RAD) sequencing analysis by applying the ezRAD technique, which is a simple and cost-effective method [12,13] for generating and examining genetic variation. This approach was applied to deep-sea candidate amphipod species selected by DNA barcoding.
Materials and methods
Sampling, DNA extraction and library preparation
We used amphipod specimens collected with baited traps (shrimp pot, conger tube), including baited traps mounted on the Edokko Mark I benthic observation system (Okamoto Glass Co., Ltd.), at three sites (Table 1). A total of 37 specimens were used for this study. Specimens were preserved by immersion in absolute ethanol followed by freezing, and genomic DNA was extracted from the pleopods of each specimen according to the manufactures protocol of the Qiagen DNeasy Blood and Tissue Kit. DNA was also extracted from some specimens preserved in formalin. Partial sequences of the COI region were determined by the method described in the ISA Technical Study No. 13 [14]. To prepare a library for ezRAD, we used a Qubit Fluorometer (ThermoFisher, Waltham, USA) to measure the DNA concentration in each DNA sample. A library for RAD-Seq was prepared following the ezRAD protocol [12, 13] using the Illumina TruSeq library preparation kit. A bioanalyzer was used to check the quality, and paired-end reads (2 x 75 base pairs) were obtained. We also sequenced the genome of one specimen using a Nextera XT DNA Sample Prep Kit (Illumina) and obtained paired-end (2 x 300 base pairs) reads. These reads were obtained using the Illumina Miseq sequencer following the manufacturer’s protocol.
Bioinformatics
We inferred 17 haplotypes from the partial COI sequences, and then we performed a BLASTN search (e-value cut-off: 1e-5) against the NCBI nt database (July, 2018) to identify possible neighboring species. We also used BOLD database (www.boldsystems.org) to infer the species of these 17 haplotypes. Then a maximum likelihood method was used to generate a tree for visualizing the genetic diversity of the amphipod species (Fig 1). For the analysis, we used a GTR + I + G model and 1,000 bootstrap replicates to estimate the statistical support for each clade. To evaluate the “barcoding gap” between intraspecific variation and interspecific/interclade divergence [8], we used the Kimura two-parameter model [15] when calculating genetic distances (Fig 2) based on representative haplotypes of the 11 groups in Fig 1 (all haplotypes excluding XI, VI, and VII which are very similar to XII) and on all sequences of Group 9 (Fig 1), a putative species that was inferred to belonging to the genus Abyssorchomene on the basis of a BLASTN search. From the DNA Data Bank of Japan (DDBJ) database, we downloaded all COI sequences of Abyssorchomene and constructed a haplotype network using the downloaded sequences along with the Abyssorchomene sequences obtained in this study. We also generated a tree via the maximum likelihood method using the 53 haplotypes inferred from all Abyssorchomene sequences (Fig 3). We used the GTR + I + G model; clade support was evaluated by 1,000 bootstrap replicates. These analyses were performed with the R software package [16] and related packages (ape, pegas, phangorn, seqinr; [17–20]). To identify molecular operational taxonomic units (MOTUs), we applied the automatic barcode gap discovery [ABGD; 21] and Bayesian implementation of the Poisson Tree Process [bPTP; 22] methods. In the ABGD, we used the Kimura two-parameter model [15] and the relative gap width (X) for X = 1.5 an X = 1 in Figs 1 and 3, respectively because only one clade was supported in the latter case with X = 1.5. In the bPTP, we constructed neighbor-joining trees and performed the analysis with the default settings in the web version (http://species.h-its.org/ptp/; MCMC generations: 100,000; thinning: 100; burn-in: 0.1). We constructed a haplotype network of seven haplotypes of Abyssorchomene sp. using the TCS network algorithm [23] in PopART v. 1.7 [24]. All sequence data have been deposited in the DDBJ database (accession nos. LC484978–LC485014).
Numbers indicate bootstrap values (1,000 replicates). One clade (Group 9, in the box) is composed of haplotypes inferred to represent a putative species.
Closed bars show intra group distances of the 18 sequences inferred to represent a single species. Open bars show intergroup distances.
Numbers indicate bootstrap values (1,000 replicates). One clade (in the box) is composed of haplotypes inferred to represent a single species.
The obtained FASTQ files of high-throughput sequencing data were filtered by using cutadapt version 1.9.1 software [25], and the reads with poor-quality bases (Q < 20) and those with lengths < 40 base pairs (bp) were discarded. Genome sequences of one Abyssorchomene sp. were assembled by using fastq-join in the ea-utils package [26]. Redundant sequences were removed by using the CD-HIT-EST program [27]. We selected sequences longer than 400 bp by using Seqkit version 0.9.3 [28] and then used these sequences as genome data for Abyssorchomene sp. To facilitate the mapping of short reads obtained by ezRAD, we processed the FASTQ files and prepared 25-bp-long sequences in FASTA files. We used the bowtie2 program (default setting) to align the FASTA sequences obtained by using ezRAD with the Abyssorchomene sp. genomic data [29]. We then used Stacks software (programs: pstacks (default setting), cstacks (-b 1 -p 4 -n 5), sstacks (-b 1 -p 4), and genotypes (-b 1)) [30] to identify single nucleotide polymorphisms (SNPs) in the obtained SAM files. Short-read data have been deposited in the DDBJ data base (accession nos. DRA008512 and DRA008890). Using the catalog files made by Stacks, we prepared a NEXUS file that included SNP loci among 10 individuals for subsequent analyses. We also used a maximum likelihood method to generate a tree with RAxML ver. 8.2.7 software [31]. For the analysis, we used the GTR-GAMMA model and 1000 bootstrap replicates to estimate the clade confidence levels.
Results and discussion
We applied DNA barcoding methods to deep-sea amphipods collected from CRCs and identified 17 haplotypes from 37 individual sequences (S1 Table). The BLASTN search indicated that almost all species belonged to the superfamily Lysianassoidea, which is ubiquitous in deep-sea areas ([10]; S2 Table). Several clades molecularly delimited via ABGD and bPTP were supported by high bootstrap values (Figs 1 and S1; Groups 1–11). Among these clades, Group 9 composed of four haplotypes (XV, VIII, XVI, and IV). These four haplotypes were similar to Abyssorchomene sp. sequences in the NCBI nt database (S2 Table). In addition, a significant difference between intragroup and intergroup genetic distances was also confirmed (“barcoding gap”; Mann–Whitney U test, p < 0.01; Fig 2). ABGD detected 8 and 13 MOTUs among the haplotypes in Figs 1 and 3, respectively. Eleven and 13 MOTUs were detected by bPTP among the haplotypes in Figs 1 and 3, respectively. Both methods supported the Group 9 as a single MOTU. We therefore selected this putative species as a candidate for the subsequent analysis because a relatively larger number of specimens was available.
We downloaded 71 Abyssorchomene sequences registered in the DDBJ database. Among these, we excluded two short sequences from our analyses. As a result, we used a total of 87 sequences: 69 downloaded sequences and 18 sequences obtained in this study via the analysis described above and inferred to be Abyssorchomene sp. From these sequences, 53 haplotypes were detected (S3 Table). Molecular analysis of these 53 haplotypes yielded several clades, one of which was composed of seven haplotypes inferred to represent a single Abyssorchomene sp. (Fig 3). MOTUs obtained by the ABGD and bPTP methods also supported this clade of Abyssorchomene sp. (Fig 3). The inclusion in this clade of the Abyssorchomene sp. sequences reported from the New Hebrides Trench in the South Pacific suggested that this Abyssorchomene sp. is widely distributed in both the North and South Pacific Oceans. The constructed haplotype network of this Abyssorchomene sp. showed a network structure that included one main haplotype and several others (Fig 4). We therefore selected this Abyssorchomene sp. as a candidate species for connectivity analysis of CRC areas in wide areas of the Pacific.
To evaluate the genetic variation of this candidate species in more detail, we performed an ezRAD analysis. The ezRAD libraries yielded on average 0.82 million 150-bp reads per individuals (S4 Table). Among these, we used the sequence data from 10 individuals (S4 Table), because after processing the data, we had obtained enough reads from these individuals for our analysis. We detected 75,265 SNPs among these 10 individuals. From the SNP polymorphisms, we inferred that all of these individuals were genetically distinct (Fig 5); this result implies that populations of this species are maintained mainly by sexual reproduction. Amphipod genera Paralicella, Abyssorchomene, and Eurythenes are reported to be cosmopolitan taxa widely distributed in abyssal zones [32], and several microsatellite markers have been developed for Paralicella tenuipes [33,34]. Considering the position of the Abyssorchomene sp. based on the tree of the COI region and the amount of polymorphism found by the ezRAD analysis, we infer that this species is also an appropriate species for use in future connectivity analyses in CRC areas. Considering the small number of specimens used in this study, an increased number of specimens would likely identify more candidate amphipod species for future connectivity analyses that would also enable us to use α and β diversity measures to evaluate community structures of deep-sea amphipods among CRC areas.
In conclusion, we succeeded in selecting a candidate amphipod species for understanding connectivity patterns around CRC areas. Connectivity among deep-sea organisms is now a matter of concern, especially in areas that are candidates for mining of marine minerals [4,5]. However, few genetic studies that would make it possible to generalize connectivity patterns have been conducted in deep-sea areas [35]. The evaluation of connectivity among mining areas is necessary before mining begins, and several connectivity analysis studies have been carried out in hydrothermal vent fields [36–38]. Nevertheless, information about connectivity in CRC areas is still lacking. The strategy as presented in this study is expected to facilitate the visualization of connectivity patterns around CRCs in deep-sea areas based on biological data. Knowledge of these patterns is essential not only for understanding the formation and maintenance of biodiversity, but also for establishing marine protected areas to minimize the effect of deep-sea mining of CRCs on the deep-sea ecosystem.
Supporting information
S1 Fig. Molecular tree based on partial sequences of the cytochrome-oxidase I gene (605 bp) of the amphipods collected from the JA06 seamount and neighboring species.
The tree was constructed by MEGA6 [41] based on genetic distances of Kimura 2-parameter model [40] and Neighbor-Joining method [39]. Bootstrap values (percentages of 1,000 replications) >70% are shown at the respective selected branches. Bar means 0.05 substitutions per site. Greek numbers indicate 17 haplotypes.
https://doi.org/10.1371/journal.pone.0228483.s001
(PDF)
S1 Table. Haplotype compositions of the COI sequences used to construct Fig 1.
https://doi.org/10.1371/journal.pone.0228483.s002
(XLSX)
S2 Table. Results of the BLASTN search against the nt database and BOLD (COI FULL database) using the COI sequences obtained in this study.
https://doi.org/10.1371/journal.pone.0228483.s003
(XLSX)
S3 Table. Haplotype compositions of the COI sequences used to construct Fig 3.
https://doi.org/10.1371/journal.pone.0228483.s004
(XLSX)
S4 Table. Details of the ezRAD analysis.
Sample names in bold type were used in our final analysis.
https://doi.org/10.1371/journal.pone.0228483.s005
(XLSX)
Acknowledgments
This study was commissioned by Agency for Natural Resources and Energy, Ministry of Economy, Trade and Industry of Japan. Computations were partially performed on the NIG supercomputer at ROIS National Institute of Genetics.
References
- 1. Clark MR, Rowden AA, Schlacher T, Williams A, Consalvey M, Stocks KI, et al. The ecology of seamounts: structure, function, and human impacts. Annu Rev Mar Sci. 2010; 2: 253–278.
- 2.
ISA. Recommendations for the guidance of contractors for the assessment of the possible environmental impacts arising from exploration for marine minerals in the Area. 2013; ISBA/19/LTC/8.
- 3.
Avise JC. Phylogeography: the history and formation of species. Harvard university press. 2000.
- 4. Baco AR, Etter RJ, Ribeiro PA, Von der Heyden S, Beerli P, Kinlan BP. A synthesis of genetic connectivity in deep-sea fauna and implications for marine reserve design. Mol Ecol. 2016; 25: 3276–3298. pmid:27146215
- 5. Taylor ML, Roterman CN. Invertebrate population genetics across Earth's largest habitat: The deep-sea floor. Mol Ecol. 2017; 26: 4872–4896. pmid:28833857
- 6. Glover AG, Wiklund H, Chen C, Dahlgren TG. Point of View: Managing a sustainable deep-sea ‘blue economy’ requires knowledge of what actually lives there. eLife. 2018; 7: e41319. pmid:30479272
- 7. Hebert PD, Gregory TR. The promise of DNA barcoding for taxonomy. Systematic Biol. 2005; 54: 852–859.
- 8. Meyer CP, Paulay G. DNA barcoding: error rates based on comprehensive sampling. PLoS Biol. 2005; 3: e422. pmid:16336051
- 9. Leray M, Yang JY, Meyer CP, Mills SC, Agudelo N, Ranwez V, et al. A new versatile primer set targeting a short fragment of the mitochondrial COI region for metabarcoding metazoan diversity: application for characterizing coral reef fish gut contents. Frontiers in Zool. 2013; 10: 34.
- 10. Ritchie H, Jamieson AJ, Piertney SB. Phylogenetic relationships among hadal amphipods of the Superfamily Lysianassoidea: Implications for taxonomy and biogeography. Deep Sea Res I. 2015; 105: 119–131.
- 11. Kishimoto K, Usui A, Yamaoka K, Yuasa M, Suzuki A, Nishimura A. Distribution Map of Marine Minerals in the Northwestern Pacific, ver. 2. Geol. Surv. Japan Misc. Map Ser. 33 (ver. 2), One sheet map with explanatory note. 2017.
- 12. Toonen RJ, Puritz JB, Forsman ZH, Whitney JL, Fernandez-Silva I, Andrews KR, et al. ezRAD: a simplified method for genomic genotyping in non-model organisms. PeerJ. 2013; 1: e203. pmid:24282669
- 13.
Knapp ISS, Puritz J, Bird C, Whitne J, Sudek M, Forsman ZH, et al. ezRAD- an accessible next-generation RAD sequencing protocol suitable for non-model organisms_v3.2. protocols.io. 2016. https://doi.org/doi.org/10.17504/protocols.io.e9pbh5n
- 14.
ISA. Deep Sea Macrofauna of the Clarion-Clipperton Zone, ISA technical study. 2015; No. 13, ISBN 978-976-8241-32-0
- 15. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980; 16: 111–120. pmid:7463489
- 16.
R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. 2015. URL http://www.R-project.org/
- 17. Paradis E, Claude J, Strimmer K. APE: analyses of phylogenetics and evolution in R language. Bioinformatics. 2004; 20: 289–290. pmid:14734327
- 18.
Charif D Lobry JR. Seqin{R} 1.0–2: a contributed package to the {R} project for statistical computing devoted to biological sequences retrieval and analysis. In Bastolla U. Porto M, Roman HE & Vendruscolo M (Eds). Structural Approaches to Sequence Evolution: Molecules, Networks, Populations, Biological and Medical Physics, Biomedical Engineering (pp. 207–232). New York: Springer Verlag. 2007.
- 19. Paradis E. pegas: an R package for population genetics with an integrated–modular approach. Bioinformatics. 2010; 26: 419–420. pmid:20080509
- 20. Schliep KP. phangorn: Phylogenetic analysis in R. Bioinformatics. 2011; 27: 592–593. pmid:21169378
- 21. Puillandre N, Lambert A, Brouillet S, Achaz G. ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Mol Ecol. 2011; 21: 1864–1877. 10.1111/j.1365-294X.2011.05239.x pmid:21883587
- 22. Zhang J, Kapli P, Pavlidis P, Stamatakis A. A general species delimitation method with applications to phylogenetic placements. Bioinformatics. 2013; 29: 2869–2876. https://doi.org/10.1093/bioinformatics/btt499 pmid:23990417
- 23. Clement M, Snell Q, Walke P, Posada D, Crandall K. TCS: estimating gene genealogies. Proc 16th Int Parallel Distrib Process Symp. 2002; 2: 184.
- 24. Leigh JW, Bryant D. POPART: full-feature software for haplotype network construction. Methods Ecol Evol. 2015; 6: 1110–1116.
- 25. Martin M. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet J. 2011; 17: 10–12.
- 26.
Aronesty E. ea-utils: "Command-line tools for processing biological sequencing data". 2011. http://code.google.com/p/ea-utils
- 27. Li W, Godzik A. Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences. Bioinformatics. 2006; 22: 1658–1659. pmid:16731699
- 28. Shen W, Le S, Li Y, & Hu F. SeqKit: a cross-platform and ultrafast toolkit for FASTA/Q file manipulation. PLoS ONE. 2016; 11: e0163962. pmid:27706213
- 29. Langmead B, Salzberg SL. Fast gapped-read alignment with Bowtie 2. Nature methods. 2012; 9: 357. pmid:22388286
- 30. Catchen JM, Amores A, Hohenlohe P, Cresko W, Postlethwait JH. Stacks: building and genotyping loci de novo from short-read sequences. G3: Genes, genomes, genetics. 2011; 1: 171–182.
- 31. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics. 2014; 30: 1312–1313. pmid:24451623
- 32. Fujii T, Kilgallen NM, Rowden AA, Jamieson AJ. Deep-sea amphipod community structure across abyssal to hadal depths in the Peru-Chile and Kermadec trenches. Mar Ecol Prog Ser. 2013; 492: 125–138.
- 33. Ritchie H, Jamieson AJ, Piertney SB. Isolation and characterization of microsatellite DNA markers in the deep-sea amphipod Paralicella tenuipes by Illumina MiSeq sequencing. J Hered. 2016; 107: 367–371. pmid:27012615
- 34. Ritchie H, Jamieson AJ, Piertney SB. Population genetic structure of two congeneric deep-sea amphipod species from geographically isolated hadal trenches in the Pacific Ocean. Deep Sea Res I. 2017; 119: 50–57.
- 35. Kiel SA. Biogeographic network reveals evolutionary links between deep-sea hydrothermal vent and methane seep faunas. Proc R Soc B: Biol Sci. 2016; 283: 20162337.
- 36. Watanabe H, Tsuchida S, Fujikura K, Yamamoto H, Inagaki F, Kyo M, et al. Population history associated with hydrothermal vent activity inferred from genetic structure of neoverrucid barnacles around Japan. Mar Ecol Prog Ser. 2005; 288: 233–240.
- 37. Yahagi T, Watanabe H, Ishibashi JI, Kojima S. Genetic population structure of four hydrothermal vent shrimp species (Alvinocarididae) in the Okinawa Trough, Northwest Pacific. Mar Ecol Prog Ser. 2015; 529: 159–169.
- 38. Mitarai S, Watanabe H, Nakajima Y, Shchepetkin AF, McWilliams JC. Quantifying dispersal from hydrothermal vent fields in the western Pacific Ocean. Proc Natl Acad Sci. 2016; 113: 2976–2981. pmid:26929376
- 39. Saitou N., & Nei M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution, 4(4), 406–425. pmid:3447015
- 40. Kimura M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of molecular evolution, 16(2), 111–120. pmid:7463489
- 41. Tamura K., Stecher G., Peterson D., Filipski A., & Kumar S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution, 30(12), 2725–2729. pmid:24132122