Figures
Fig 3 is incorrect. The authors have provided a corrected version here. The publisher apologizes for the error.
A) The anti-AyNCXA antibodies recognize a ~100 kDa and ~75 kDa protein in homogenized A. yongei tissue. Both bands are eliminated when the antibody is pre-absorbed with the epitope peptide overnight, and neither band is present when the membrane is incubated with the pre-immune serum. All sample wells contain the same amount of protein and all three Western Blot images were taken at the same exposure. B) Immunofluorescence microscopy of A. yongei tissue reveals AyNCXA is present in all four tissue layers, including the calicodermis. C) Pre-absorption of antibodies with antigen peptide eliminates signal at the same exposure, confirming antibody specificity.
Reference
- 1. Barron ME, Thies AB, Espinoza JA, Barott KL, Hamdoun A, Tresguerres M (2018) A vesicular Na+/Ca2+ exchanger in coral calcifying cells. PLoS ONE 13(10): e0205367. https://doi.org/10.1371/journal.pone.0205367 pmid:30379874
Citation: The PLOS ONE Staff (2018) Correction: A vesicular Na+/Ca2+ exchanger in coral calcifying cells. PLoS ONE 13(12): e0209734. https://doi.org/10.1371/journal.pone.0209734
Published: December 19, 2018
Copyright: © 2018 The PLOS ONE Staff. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.