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Open Access

Peer-reviewed

Research Article

The absence of specific yeast heat-shock proteins leads to abnormal aggregation and compromised autophagic clearance of mutant Huntingtin proteins

  • Ryan Higgins,

    Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

    Affiliation Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, Florida, United States of America

    ⨯
  • Marie-Helene Kabbaj,

    Roles Data curation, Investigation, Methodology

    Affiliation Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, Florida, United States of America

    ⨯
  • Alexa Hatcher,

    Roles Investigation, Methodology

    Affiliation College of Nursing, Florida State University, Tallahassee, Florida, United States of America

    ⨯
  • Yanchang Wang

    Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Resources, Supervision, Visualization, Writing – review & editing

    yanchang.wang@med.fsu.edu

    Affiliation Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, Florida, United States of America

    ⨯

Abstract

The functionality of a protein depends on its correct folding, but newly synthesized proteins are susceptible to aberrant folding and aggregation. Heat shock proteins (HSPs) function as molecular chaperones that aid in protein folding and the degradation of misfolded proteins. Trinucleotide (CAG) repeat expansion in the Huntingtin gene (HTT) results in the expression of misfolded Huntingtin protein (Htt), which contributes to the development of Huntington’s disease. We previously found that the degradation of mutated Htt with polyQ expansion (Htt103QP) depends on both ubiquitin proteasome system and autophagy. However, the role of heat shock proteins in the clearance of mutated Htt remains poorly understood. Here, we report that cytosolic Hsp70 (Ssa family), its nucleotide exchange factors (Sse1 and Fes1), and a Hsp40 co-chaperone (Ydj1) are required for inclusion body formation of Htt103QP proteins and their clearance via autophagy. Extended induction of Htt103QP-GFP leads to the formation of a single inclusion body in wild-type yeast cells, but mutant cells lacking these HSPs exhibit increased number of Htt103QP aggregates. Most notably, we detected more aggregated forms of Htt103QP in sse1Δ mutant cells using an agarose gel assay. Increased protein aggregates are also observed in these HSP mutants even in the absence Htt103QP overexpression. Importantly, these HSPs are required for autophagy-mediated Htt103QP clearance, but are less critical for proteasome-dependent degradation. These findings suggest a chaperone network that facilitates inclusion body formation of misfolded proteins and the subsequent autophagic clearance.

Citation: Higgins R, Kabbaj M-H, Hatcher A, Wang Y (2018) The absence of specific yeast heat-shock proteins leads to abnormal aggregation and compromised autophagic clearance of mutant Huntingtin proteins. PLoS ONE 13(1): e0191490. https://doi.org/10.1371/journal.pone.0191490

Editor: Jeffrey L. Brodsky, University of Pittsburgh, UNITED STATES

Received: June 30, 2017; Accepted: January 5, 2018; Published: January 18, 2018

Copyright: © 2018 Higgins et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the paper and its Supporting Information files.

Funding: This study was supported by RO1GM102115, National Institutes of Health to YW, https://www.nih.gov/. This work is also supported by a FSU CRC planning grant to YW. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Introduction

Misfolded proteins are prone to aggregate and protein aggregates can cause various deleterious effects within cells. Protein aggregation is linked to several neurodegenerative disorders, including Huntington, Alzheimer, Parkinson and prion diseases [1, 2]. Protein aggregation also contributes to the development of diabetes and cancer [3, 4]. Cells have evolved protein quality control mechanisms to combat the potential toxic effects of aggregated proteins. First, misfolded proteins can be refolded into functional proteins. Second, misfolded proteins can be ubiquitinated and targeted for degradation by proteasomes. In addition, aggregated proteins can be cleared through the autophagy pathway. Often the first line of defense against the generation of unfolded/misfolded proteins depends on heat shock proteins (HSPs), which are abundant molecular chaperones involved in protein folding, refolding, disaggregation and degradation [5]. Thus, it is of interest to understand the roles of chaperones in the response to the expression of aggregation-prone proteins.

Hsp70 chaperones are highly abundant ATPases that bind to hydrophobic regions of substrates to facilitate protein folding [6]. When Hsp70 is bound to ATP, the substrate binding affinity is low, but upon ATP catalysis, ADP-bound Hsp70 shows a high substrate binding affinity [7]. Nucleotide-exchange factors (NEFs) are necessary for the exchange of ADP with ATP, and yeast cells have three distinct types of NEFs, including Hsp110 (Sse1 and Sse2), HspBP1 (Fes1), and a bag domain-containing protein (Snl1). Sse1 is the most abundant Hsp70 NEF, followed by Fes1 at approximately one-fifth the level of Sse1, whereas very low levels of Sse2 and Snl1 are detected [8]. In accordance with their expression levels, most of the Hsp70 NEF functions are associated with Sse1 and Fes1 [9]. Another layer of the chaperone network is the Hsp40 co-chaperones, which enhance the intrinsically low ATPase activity of Hsp70s [10]. Hsp40 co-chaperones also contribute to the substrate specificity of Hsp70s [11]. Therefore, Hsp40s, Hsp70s and NEFs comprise an ATP-dependent chaperone network that maintains proteostasis by facilitating the refolding or degradation of misfolded proteins.

Although the chaperone network is highly efficient, misfolded proteins still aggregate due to various causes, such as genetic mutations and environmental and oxidative stresses. Disaggregation complexes are needed to resolve these aggregates. In yeast cells, the AAA+ ATPase Hsp104 is a powerful disaggregase that is recruited to protein aggregates with the assistance of Hsp70 and Hsp40 to facilitate disaggregation [12]. Recent studies have also implicated Hsp110 (Sse1) in facilitating protein disaggregation in both yeast and mammalian cells [13–15]. Depending on their misfolded state, disaggregated proteins are destined for either refolding or degradation [12]. Thus, disaggregase machineries are critical in protecting cells from the harmful effects of protein aggregation.

Accumulation of protein aggregates is a hallmark of several neurodegenerative diseases [16, 17]. Huntington’s disease (HD) is a dominant neurodegenerative disorder caused by a trinucleotide (CAG) repeat expansion in exon I of the HTT gene, which results in terminal misfolding and aggregation of Htt proteins. HD is often a late-onset disease which is likely attributed to decreased activity of protein quality control systems, including the ubiquitin proteasome system (UPS) [18–21]. In neuronal cells, less efficient UPS activity is linked to neurodegenerative diseases including HD [22]. Budding yeast has been used as a model organism to study the cellular response to the expression of misfolded human disease proteins. In yeast cells, overexpression of mutated Huntingtin with 103 polyQ expansion and the proline-rich domain (Htt103QP) results in aggregation, making it an ideal substrate to study the process of protein aggregation and clearance [23, 24]. Interestingly, overexpression of Htt103QP is not toxic to yeast cells because it is sequestered and deposited into cytoprotective inclusion bodies (IB) which can be cleared via autophagy [25–27]. However, the role of chaperone proteins in the clearance of mutated Huntingtin remains largely unexplored.

In this study, we identified a chaperone network that is required for Htt103QP IB formation and the subsequent autophagic degradation in yeast cells. The cytosolic Hsp70 Ssa chaperones, the Hsp70 NEFs Sse1 and Fes1, and Hsp40 co-chaperone Ydj1 are required for Htt103QP IB formation. Interestingly, in cells lacking Sse1 and Fes1, Htt103QP aggregation is accelerated. In addition, the aggregation of other proteins is also more pronounced as evidenced by the formation of Hsp104-GFP foci in the absence of Htt103QP expression. Surprisingly, sse1Δ, fes1Δ and ydj1-151 mutants exhibit no significant delay in proteasome-dependent degradation of Htt103QP. However, we found that the recognition of Htt103QP aggregates by the autophagy machinery was much less efficient in sse1Δ, fes1Δ and ydj1-151 mutants, as Htt103QP co-localization with the autophagosomal marker Atg8 was significantly reduced. As a result, the rate of autophagic degradation of Htt103QP was substantially decreased in these mutants. Therefore, our data support the conclusion that the absence of a particular group of HSPs results in abnormal aggregation of misfolded proteins, which compromises their clearance through the autophagy pathway.

Results

Sse1 and Fes1 are required for mutant Huntingtin inclusion body formation

Inclusion body (IB) formation is an active cytoprotective process that sequesters misfolded protein species [25–28]. In budding yeast Saccharomyces cerevisiae, overexpression of mutant Huntingtin with 103 polyQ repeat and the proline-rich domain (Htt103QP) from a galactose promoter (PGALFlag-Htt103QP-GFP) leads to IB formation [23, 24]. However, many of the components necessary for IB formation have yet to be discovered. From a genome-wide genetic screen, we identified dsk2Δ mutant that showed compromised IB formation and was sensitive to Htt103QP expression [26]. From this screen, we also found that sse1Δ mutant exhibited slow growth on galactose plates, but this slow growth phenotype was independent of Htt103QP overexpression, as the mutant cells grew slowly on both galactose and glucose plates (Fig 1A). A previous study found that Sse1 associates with Htt103QP aggregates formed in yeast cells [25], therefore, we examined Htt103QP IB formation in sse1Δ mutant. For this experiment, cells were incubated in galactose medium for 16 hrs to induce Htt103QP-GFP overexpression. The majority of wild-type (WT) cells exhibited one single large inclusion body marked by GFP, but sse1Δ cells had multiple Htt103QP aggregates, which were often smaller and morphologically different from the inclusions in WT cells (Fig 1B). Moreover, the GFP intensity of the aggregates in sse1Δ cells was considerably less than those in WT cells (Fig 1C). We found that majority of WT cells (79%) had only one IB, whereas 88% of sse1Δ mutant cells exhibited three or more aggregates, with an average around eight aggregates in a sse1Δ cell (Fig 1E and 1F). Therefore, sse1Δ mutant cells show an increased number of aggregates and exhibit a clear IB formation defect.

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Fig 1. Cytosolic Hsp70 nucleotide exchange factors Sse1 and Fes1 are required for Htt103QP inclusion body (IB) formation.

(A) Htt103QP overexpression and the growth of WT and sse1Δ cells. Cells containing PGALFlag-Htt103QP-GFP were grown to saturation, 10-fold diluted, and spotted onto glucose (YPD) or galactose (YEPG) (yeast extract peptone and galactose) plates. The plates were incubated at 30°C for 2 days. (B) Confocal DIC and fluorescent images showing Htt103QP IB formation. WT and sse1Δ cells with PGALFlag-Htt103QP-GFP were incubated at 30°C in YEPG medium for 16 hrs to induce Htt103QP-GFP expression. The cells were fixed by paraformaldehyde for 5 min and then resuspended in PBS buffer for confocal microscopy. Scale bar = 5μm. (C) 2.5-D analysis showing the intensity of fluorescent GFP peaks of the images from (B). Software used was Zen Blue from Zeiss. (D) Htt103QP IB formation in yeast mutants lacking Hsp70 nucleotide exchange factors (NEFs). Cells with the indicated genotypes were grown and treated as described above and then examined using an EVOS fluorescence microscope. Representative fluorescent images are shown for WT, snl1Δ, sse1Δ, sse2Δ, fes1Δ, and sse1Δ fes1Δ cells expressing Htt103QP-GFP. Scale bar = 5μm. (E) Cells from (D) were quantified for the number of aggregates in each cell: 1, 2 or >2 (n = 100 cells). The results are the average of three independent experiments. (F) The average number of aggregates in WT, sse1Δ, fes1Δ, and sse1Δ fes1Δ cells from (D) were quantified (n > 40 cells). The results are the average of three independent experiments. * indicates statistical comparison between WT and mutants. p < .0001 in all instances.

https://doi.org/10.1371/journal.pone.0191490.g001

Sse1 is one of the four cytosolic Hsp70 nucleotide exchange factors (NEF) in yeast cells, with the other three being Sse2, Fes1 and Snl1 [9]. Therefore, we examined if the others were also required for Htt103QP IB formation. sse2Δ and snl1Δ mutant cells exhibited normal IB formation after Htt103QP induction, but fes1Δ mutant showed a clear IB formation defect as indicated by the increased number of GFP foci, albeit less pronounced than sse1Δ cells (Fig 1D). Approximately 35% of fes1Δ mutant cells were still able to form a single large IB like WT cells, but other cells contained additional smaller aggregates (Fig 1D and 1E). This is consistent with the average number of aggregates in fes1Δ cells being more than WT but less than sse1Δ cells (Fig 1F). Since both sse1Δ and fes1Δ mutants exhibited IB formation defect, we speculated that a double mutant might exacerbate the phenotype. Interestingly, the sse1Δ fes1Δ double mutants did not show exacerbated phenotype, but instead exhibited a phenotype more similar to fes1Δ cells (Fig 1D, 1E and 1F). This might be attributed to the dramatic increase in heat shock response in sse1Δ fes1Δ double mutants [9], as enhanced expression of other HSPs may suppress the severity of the sse1Δ fes1Δ phenotype. Taken together, these results indicate that cytosolic Hsp70 NEF Sse1, and Fes1 to a lesser extent, participates in IB formation in yeast cells overexpressing Htt103QP.

Deletion of either SSE1 or FES1 results in accelerated Htt103QP aggregation

Sse1 has previously been implicated in preventing protein aggregation by assisting in protein folding and facilitating protein disaggregation [15, 29, 30]. In this context, we tested whether sse1Δ and fes1Δ mutants changed the rate of aggregation after Htt103QP overexpression. Cells with PGALFlag-Htt103QP-GFP were grown to mid-log phase in non-inducible raffinose containing medium, and then galactose was added into the medium to induce Htt103QP overexpression. Cells were collected over time to visualize the appearance of GFP foci, which indicate Htt103QP aggregate formation. We found that both sse1Δ and fes1Δ mutants exhibited accelerated aggregation of Htt103QP compared to WT cells (Fig 2A). In cells lacking Sse1 and Fes1, visible aggregates appeared at 1 and 2 hr after Htt103QP induction, respectively, while visible aggregates began to appear in WT cells after 3 hr induction. Furthermore, the average number of aggregates was substantially higher in both mutants with sse1Δ mutants averaging nearly four per cell after 5 hr galactose induction (Fig 2B). To validate these microscopic observations biochemically, we performed SDS agarose gel electrophoresis (SDS-AGE) [31]. Htt103QP species with higher molecular weight were observed in sse1Δ mutants compared to WT after 3 hr galactose induction, suggesting more pronounced Htt103QP aggregation in sse1Δ cells (Fig 2C). As expected, increased aggregated Htt103QP was not observed in sse2Δ and snl1Δ mutant cells. Surprisingly, we did not observe an appreciable increase in Htt103QP aggregates in fes1Δ cells using SDS-AGE (Fig 2C), indicating that the biochemical nature of the aggregates observed in sse1Δ and fes1Δ is likely different. These results suggest that Sse1 and Fes1 aid in preventing the aggregation of Htt103QP in yeast cells, with Sse1 seemingly playing a more predominant role.

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Fig 2. Cells lacking Sse1 and Fes1 exhibit accelerated Htt103QP aggregate formation.

(A) WT, sse1Δ, and fes1Δ cells with PGALFlag-Htt103QP-GFP plasmid were grown in non-inducible raffinose medium (YEP + Raffinose) to mid log phase, and then galactose was added to induce the overexpression of Flag-Htt103QP-GFP. Cells were collected every hour for microscopy using the EVOS microscope. Representative DIC and fluorescence images are shown for Htt103QP aggregate formation. Scale bar = 5μm. The number of aggregates in each cell were quantified. The percentage of cells with 1, 2 or >2 aggregates (n = 100 cells) is shown. (B) Quantitation of the average number of aggregates per cell in WT, sse1Δ and fes1Δ cells. Averages are from three independent experiments (n > 50 cells). (C) Detection of Htt103QP protein aggregates using agarose gel electrophoresis. WT and mutant cells were grown in non-inducible raffinose medium (R). After galactose addition, the cells were incubated for 3 hr (G). Protein samples were prepared as described in Materials and Methods and subjected to SDS agarose gel electrophoresis (SDS-AGE) and SDS-PAGE. Two separate WT and sse1Δ strains are shown for reproducibility (left). The results for WT, sse2Δ, fes1Δ, and snl1Δ mutants are shown in the right panel. Pgk1: loading control.

https://doi.org/10.1371/journal.pone.0191490.g002

Hsp70 and Hsp40 chaperones facilitate Htt103QP IB formation

Sse1 and Fes1 are NEFs that replace ADP with ATP on the cytosolic Ssa subfamily of Hsp70 [32, 33]. This subfamily includes Ssa1, Ssa2, Ssa3 and Ssa4, and they interact with both Sse1 and Fes1 [34]. Therefore, we determined whether these chaperones played a role in Htt103QP IB formation. We first examined the IB formation in each single deletion mutant, but no obvious defect was observed (S1A Fig). We speculated the efficient Htt103QP IB formation in each single mutant is likely due to the redundant function of Hsp70 protein family. To test this possibility, we used a strain lacking SSA2, SSA3 and SSA4 genes but expressing temperature-sensitive ssa1-45 [35]. We found that this mutant showed a significant Htt103QP IB formation defect when incubated at 35°C. Many of the mutant cells exhibited diffuse Htt103QP-GFP, while others showed numerous small aggregates (Fig 3A). Due to the diffuse nature of Htt103QP in many mutant cells, quantitation was not performed. These results indicate that cytosolic Hsp70 proteins are critical for Htt103QP IB formation.

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Fig 3. Hsp70 and Hsp40 chaperones participate in Htt103QP IB formation.

(A) Htt103QP IB formation in yeast Hsp70 Ssa family mutants. Fluorescent images of SSA1-WT and SSA1-45 cells expressing Flag-Htt103QP-GFP at 35°C for 16 hr. Two separate fluorescent images for ssa1-45 mutants indicate two phenotypes. Scale bar = 5μm. (B) Htt103QP IB formation in Hsp40 co-chaperone mutant ydj1-151. Fluorescent images of WT and ydj1-151 cells expressing Flag-Htt103QP-GFP for 16 hrs at permissive (25°C) and semi-permissive (30°C) temperatures. Cells were quantified for the number of aggregates in each cell: 1, 2 or >2 (n = 100 cells). The results are the average of three independent experiments. Scale bar = 5μm. (C) Htt103QP IB formation in Hsp40 co-chaperone mutant sis1-85. Fluorescent images of SIS1-HA and SIS1-85-HA strains expressing Htt103QP-GFP for 16 hrs at permissive (25°C) and restrictive (37°C) temperatures. Scale bar = 5μm. All microscopy in this figure was performed on the EVOS microscope.

https://doi.org/10.1371/journal.pone.0191490.g003

We next examined the role of Hsp40 chaperones in Htt103QP IB formation. Hsp40 co-chaperone proteins stimulate the ATPase activity of Hsp70 to facilitate complex formation between Hsp70 and client proteins [11]. Previous work indicates that Hsp40 co-chaperone proteins Sis1 and Ydj1 physically interact with Sse1 [34]. Therefore, we examined IB formation in the absence of Hsp40 co-chaperones Ydj1 and Sis1. Because YDJ1 deletion mutant is very sick and SIS1 is an essential gene, we used temperature sensitive mutants for this experiment [36, 37]. The non-permissive temperature of ydj1-151 strain is 37°C, but multiple Htt103QP aggregates were observed in the mutant cells even when incubated at 25°C, and the phenotype was further exacerbated at 30°C (Fig 3B). The temperature sensitive sis1-85 mutant cells failed to aggregate any Htt103QP at non-permissive 37°C (Fig 3C), which is likely attributable to its function in [RNQ+] maintenance, as [RNQ+] is essential for the aggregation of mutant Huntingtin proteins in yeast cells [37, 38]. Therefore, Ydj1 co-chaperone is required for Htt103QP IB formation.

In addition to the Ssa family, other chaperones are also known to interact with either Sse1 or Fes1, such as Ssb1, Sti1, Hsp42, Hsp82 and Hsc82 according to the Saccharomyces genome database. We examined the Htt103QP IB formation in yeast mutants lacking these proteins, none of which exhibited an obvious defect in IB formation (S1C Fig). Taken together, these results suggest that a chaperone network is required to facilitate Htt103QP IB formation, and this network includes the Ssa class of Hsp70s, NEFs Sse1 and Fes1, and the Hsp40 co-chaperone Ydj1.

Analysis of protein aggregation in HSP mutants using disaggregase Hsp104 as a marker

The function of the yeast AAA+ ATPase Hsp104 in protein disaggregation is well established [12]. Previous studies have demonstrated that Hsp104 functions with Hsp40 and Hsp70 to facilitate protein disaggregation [39, 40], whereas more recent work indicates that Hsp110 (Sse1 and Sse2) aids Hsp104 in disaggregation of protein aggregates [13, 41]. Therefore, we determined whether Sse1 and Fes1 recruit Hsp104 to Htt103QP aggregates for disaggregation. For this experiment, WT, sse1Δ, and fes1Δ cells with HSP104-GFP PGALFlag-Htt103QP-mApple were grown in galactose medium for 6 hr to induce Htt103QP overexpression. Surprisingly, the co-localization of Hsp104-GFP and Htt103QP-mApple was similar in WT, sse1Δ and fes1Δ cells (Fig 4A), even though the mutants contain more aggregates. This observation indicates that NEFs Sse1 and Fes1 are not necessary for Hsp104 to recognize Htt103QP aggregates. We also examined the colocalization of Hsp104-GFP with Htt103QP-mApple in sse2Δ and ydj1-151 mutants, no colocalization defect was observed, which is similar to sse1Δ and fes1Δ mutants (Fig 4A). Although Hsp104 seems to recognize Htt103QP aggregates efficiently in these mutants, further biochemical studies are needed to determine the disaggregase functionality of Htt103QP-associated Hsp104 in these mutants.

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Fig 4. Hsp104-GFP positive aggregates are increased in sse1Δ and ydj1-151 mutants.

(A) Hsp104 recruitment to Htt103QP aggregates is independent of chaperones. Cell with indicated genotypes were grown in non-inducible raffinose medium to mid-log phase then galactose was added. Images were taken after incubation for 3 hr. Representative fluorescence images show cellular localization of Hsp104-GFP and Htt103QP-mApple. DIC and merged images are also shown. Scale bar = 5μm. (B) Constitutive appearance of Hsp104-GFP positive aggregates in sse1Δ and ydj1-151 mutants. Cells with indicated genotypes were grown in raffinose or galactose containing medium to mid-log phase. Representative fluorescence images are shown. WT cells show predominantly nuclear localized Hsp104-GFP in the absence of Htt103QP overexpression. Scale bar = 5μm. (C) Cells from (B) were quantified based on the presence of Hsp104-GFP positive aggregate(s). Results are the average of three independent experiments (n = 100 cells). All images in this figure was obtained using the EVOS microscope.

https://doi.org/10.1371/journal.pone.0191490.g004

When examining the Hsp104 –Htt103QP colocalization, WT cells showed enriched nuclear localization of Hsp104-GFP with very few cytoplasmic GFP foci prior to Htt103QP induction (S2 Fig). The observation regarding the enriched Hsp104-GFP nuclear localization under non-stressed conditions differs from the findings from the Glover lab [42], but is consistent with findings from the Wendland lab [43]. These differences are most likely attributable to N-terminal (the Glover lab) vs C-terminal GFP tagging of Hsp104 (the Wendland lab and this study). Strikingly, cytoplasmic Hsp104-GFP foci were observed in sse1Δ and ydj1-151 mutants, and to a lesser extent fes1Δ, even before Htt103QP induction, indicating aggregate formation (Fig 4B). For example, 91% sse1Δ mutant cells contained at least one cytoplasmic Hsp104-GFP positive foci. These foci were also observed in ydj1-151 mutant even at permissive temperature 25°C and drastically increased at 30°C (Fig 4B and 4C). This phenomenon was not observed in sse2Δ cells, which is consistent with the normal Htt103QP IB formation in these cells. Taken together, these results suggest that Sse1, Fes1 and Ydj1 are chaperones critical to prevent protein aggregation and/or promote aggregate disassembly.

Chaperones play a minor role in proteasomal degradation of Htt103QP

Mutated Huntingtin with polyQ expansion can be degraded via both UPS and autophagy pathways [44]. We speculated that the accelerated aggregation of Htt103QP in some HSP mutants could be a result of compromised protein degradation. Therefore, we examined proteasome-dependent degradation of Htt103QP in sse1Δ and fes1Δ mutant cells. We have previously shown that Htt103QP is efficiently degraded via the proteasome using a shut-off assay [26]. Here, we confirmed the efficient degradation of Htt103QP after 1 hr induction in galactose medium followed by expression shut-off by adding glucose. Inhibition of proteasome activity with MG132 blocked Htt103QP degradation (S3A Fig). In contrast, deletion of autophagy genes, atg7Δ and atg8Δ, had little effect on the degradation Htt103QP using the shut-off assay (S3B Fig). Therefore, the rapid disappearance of Htt103QP protein in WT cells after short-time induction depends on the UPS, but not the autophagy pathway. We next examined proteasome-dependent degradation of Htt103QP in yeast mutants lacking Hsp70 NEFs. Interestingly, we observed no significant delay in proteasomal degradation of Htt103QP in NEF mutants, sse1Δ, fes1Δ, sse2Δ, and snl1Δ (S3C and S3D Fig). In addition, we found no obvious delay in proteasomal degradation of Htt103QP in ydj1-151 or sis1-85 mutants at restrictive temperature 37°C (S3E Fig). Sis1 has been shown to be required for the proteasomal degradation of endogenous misfolded proteins when fused with a degron sequence from Ndc10 [45]. Since Htt103QP is expressed as a misfolded protein without this degron sequence, we speculate that Sis1 plays distinct roles for the degradation of different substrates. Similarly, no obvious Htt103QP degradation defect was observed in mutant cells lacking other chaperone proteins known to interact with Sse1 (S1B and S1D Fig). The efficient Htt103QP degradation in sse1Δ, fes1Δ, and ydj1-151 mutants is surprising, given the accelerated Htt103QP aggregation observed in these mutants. To test the possibility that this proteasome-mediated degradation of Htt103QP could only be for the soluble fraction, we performed a sedimentation as previously described [46]. We found that after 2 hr induction nearly all of the Htt103QP proteins still remained soluble in both WT and sse1Δ mutants (S4A Fig). We speculate that the Htt103QP aggregates observed in sse1Δ mutant cells after short-time induction could be soluble, which enables efficient proteasomal degradation of Htt103QP.

Protein ubiquitination is a targeting signal for proteasome-dependent degradation [47]. Previous studies indicate that both Sse1 and Fes1 are involved in Hsp70-dependent ubiquitination and degradation of misfolded proteins [48–52]. Because the proteasomal degradation was normal in these mutants, we reasoned that Htt103QP ubiquitination should not be compromised in these mutants. We examined Htt103QP ubiquitination in sse1Δ mutant as it exhibited the most severe phenotype. As expected, sse1Δ mutant exhibited similar Htt103QP ubiquitination compared to WT cells (S4B Fig). These results indicate that while some chaperone proteins prevent the rapid aggregation Htt103QP, they are not required for its ubiquitination and efficient proteasomal degradation.

Autophagic degradation of Htt103QP is impaired in sse1Δ, fes1Δ and ydj1-151 mutant cells

Previous studies have analyzed the roles of chaperone proteins in IB formation and alleviation of toxic effects of aggregation-prone proteins [22, 53–56]. The chaperone-mediated autophagy (CMA) pathway has been shown to target soluble cytosolic proteins containing KFERQ-like-motifs for autophagic degradation in mammalian cells [57]. However, very few studies have directly analyzed the role of chaperone proteins in the autophagic degradation of mutant Huntingtin protein. We recently showed that Htt103QP is efficiently degraded via autophagy in yeast cells [26]. Thus, we used a similar protocol to analyze this process in sse1Δ and fes1Δ mutant cells. Htt103QP-GFP expression was induced in galactose medium for 16 hr, then glucose and DNA synthesis inhibitor hydroxyurea (HU) were added to the medium to shut off Htt103QP-GFP expression and prevent cell division, respectively. After glucose and HU were added for 30 min, vacuolar GFP localization was observed in some WT cells, indicating the transport of Htt103QP-GFP into the vacuole via the autophagy pathway. After 90 min incubation, only 26% of WT cells still contained Htt103QP aggregate(s), but 72% of sse1Δ cells contained aggregates (Fig 5A and 5C). Therefore, we conclude that sse1Δ mutant cells exhibit a significant autophagic degradation defect for Htt103QP. We also found that fes1Δ mutants exhibited impaired autophagic degradation of Htt103QP. After glucose and HU were added for 120 min, 53% of fes1Δ cells showed Htt103QP-GFP aggregates, compared to 19% in WT cells (Fig 5B and 5D). These results indicate the critical role of chaperone proteins in the efficient autophagic clearance of misfolded proteins.

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Fig 5. The autophagic degradation of Htt103QP is compromised in sse1Δ and fes1Δ mutants.

(A) The autophagic disposal of Htt103QP in WT and sse1Δ mutant cells. pep4Δ VPH1-mApple (WT) and sse1Δ pep4Δ VPH1-mApple (sse1Δ) cells containing Flag-Htt103QP-GFP were grown in galactose medium (YEPG) at 30°C for 16 hr. Glucose was then added to shut off Htt103QP expression, and 100 mM hydroxyurea (HU) was added to block cell cycle progression. Representative fluorescent images are shown for the localization of Htt103QP-GFP and Vph1-mApple before and after addition of glucose and HU. Vph1-mApple was used to mark vacuole structure. Pep4 is a vacuolar protease, and pep4Δ mutant was used to slow degradation inside the vacuole to enhance visualization. (B) Htt103QP autophagic disposal in WT and fes1Δ mutant cells. A similar protocol was used to examine the vacuolar localization of Htt103QP-GFP. (C and D) Quantitation of cells with Htt103QP IB or aggregates localized outside the vacuole over time. The cells from (A) and (B) are used for the quantitation. The quantified results (three independent repeats) were obtained by counting the number of cells containing at least one IB or aggregate (n = 100). All microscopy in this figure was performed on the EVOS microscope.

https://doi.org/10.1371/journal.pone.0191490.g005

Aggregated proteins can be degraded through selective autophagy, which involves the recognition and sequestration of the cargo within the autophagosome, a key structure in autophagy. An autophagosome is a double membrane vesicle that delivers cytoplasmic components to lysosomes/vacuoles. Atg8 is a protein anchored to the autophagosomal membrane and is required for autophagosome formation [58]. We speculated that the selective autophagy machinery may not be able to efficiently recognize the Htt103QP aggregates in sse1Δ and fes1Δ mutants, which could contribute to the defect in clearance. To test this possibility, we examined the co-localization of Htt103QP with Atg8. For this purpose, we introduced a plasmid expressing GFP-ATG8 into yeast cells with PGALFlag-Htt103QP-mApple, and the cells were incubated in galactose medium for 16 hr to induce Htt103QP overexpression. Indeed, we found a substantial decrease in the co-localization of Htt103QP-mApple aggregates with GFP-Atg8 in sse1Δ and fes1Δ mutants (Fig 6A). The percentage of total Htt103QP aggregates that co-localize with Atg8 was 11% in sse1Δ cells compared to 70% in WT cells (Fig 6B). Importantly, this decrease was not due to defects in autophagosome formation in the mutants, as the frequency of visible autophagosomes (GFP-Atg8 foci) was similar in WT and sse1Δ cells incubated in galactose and glucose media (Fig 6C). In addition, starvation-induced non-selective autophagy was normal, and possibly even more efficient, in sse1Δ and fes1Δ mutants as indicated by the production of monomeric GFP generated from GFP-Atg8 cleavage after starvation [59] (Fig 6D). Taken together, these results indicate that Sse1 and Fes1 promote autophagy-mediated degradation of Htt103QP by allowing the effective recognition of the aggregates by the selective autophagy machinery.

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Fig 6. Sse1 and Fes1 are required for efficient recognition of Htt103QP by autophagy machinery.

(A) The co-localization of Htt103QP and Atg8 in WT, sse1Δ, and fes1Δ cells. Cells containing GFP-ATG8 and PGALFlag-Htt103QP-mApple were grown in galactose medium YEPG for 16 hrs and fixed for microscopy. Representative fluorescence images show the localization of Htt103QP-mApple and GFP-Atg8. Scale bar = 5μm. (B) Quantitation of total number of Htt103QP aggregates colocalized with GFP-Atg8 in WT, sse1Δ, and fes1Δ cells (n = 100 IB/aggregates). Quantities indicate the average of three independent experiments. * indicates statistical comparison. p < .001. (C) Quantitation of the number of GFP-Atg8 autophagosomes present in WT, sse1Δ and fes1Δ cells incubated in glucose or galactose medium (n = 100 cells). Quantities indicate the average of three independent experiments. (D) Sse1 and Fes1 are not required for starvation-induced autophagy. WT, sse1Δ, and fes1Δ cells with GFP-ATG8 were grown in SC -TRP medium, and then shifted to nitrogen-deficient medium (SD-N) for 24 hr. The cells were collected to detect the presence of GFP-Atg8 and the cleavage product, monomeric GFP, using anti-GFP antibody. Pgk1: loading control. The EVOS microscope was used to obtain the images in this figure.

https://doi.org/10.1371/journal.pone.0191490.g006

Since ydj1-151 mutants exhibit similar IB formation defects as sse1Δ and fes1Δ mutants, we next examined if ydj1-151 cells exhibited an autophagy defect as well. For this experiment, Htt103QP expression was induced in WT and ydj1-151 cells in galactose medium at 25°C for 16 hrs, then the cultures were shifted to semi-permissive temperature 32°C for 15 minutes before addition of glucose and HU to shut off Htt103QP expression and block cell cycle progression, respectively. After glucose addition for 120 min, only 16% of WT cells contained Htt103QP aggregates and most of the cells showed obvious GFP signal in the vacuole, indicating successful autophagy. In ydj1-151 mutants, however, 61% of cells contained at least one Htt103QP aggregate, and the vacuolar GFP signal was much less frequent (Fig 7A and 7B). Consistent with the lack of vacuole localization, the frequency of Htt103QP aggregates colocalizing with GFP-Atg8 was dramatically reduced in ydj1-151 mutant at semi-permissive temperature 32°C (Fig 7C and 7D). The frequency of visible GFP-Atg8 autophagosomes in WT and ydj1-151 mutant cells incubated in galactose or glucose media was similar, indicating that the autophagic degradation defect in ydj1-151 mutant was not due to defective autophagosome formation (Fig 7E). Taken together, these results indicate that chaperone proteins Sse1, Fes1, and Ydj1 are required for the recognition of Htt103QP aggregates by Atg8 and the subsequent autophagosome formation. Therefore, cells lacking these chaperones show a significant defect in autophagic clearance of Htt103QP.

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Fig 7. Hsp40 co-chaperone Ydj1 is required for efficient autophagic degradation of Htt103QP.

(A) Htt103QP autophagy in WT and ydj1-151 mutant cells. pep4Δ VPH1-mApple (WT) and ydj1-151 pep4Δ VPH1-mApple (ydj1-151) cells containing PGALFlag-Htt103QP-GFP were grown in galactose medium (YEPG) at 25°C for 16 hrs. Cells were then shifted to semi-permissive temperatures (32°C) for 10 min prior to glucose and hydroxyurea (HU) addition. Representative fluorescent images are shown for the localization of Htt103QP-GFP and Vph1-mApple (vacuolar marker) before and after addition of glucose and HU. Scale bar = 5μm. (B) Quantitation of cells with Htt103QP IB or aggregates localized outside the vacuole over time. Results from three independent experiments were used for the quantitation. The quantified results were obtained by counting the number of cells containing at least one IB or aggregate (n = 100). (C) The co-localization of Htt103QP and Atg8 in WT and ydj1-151 cells. Cells containing GFP-ATG8 and PGALFlag-Htt103QP-mApple were grown in galactose medium YEPG for 16 hrs at 32°C and fixed for microscopy. Representative fluorescence images show the localization of Htt103QP-mApple and GFP-Atg8. (D) Quantitation of total number of Htt103QP aggregates colocalized with GFP-Atg8 in WT and ydj1-151 cells (n = 100 IB/aggregates). Quantities indicate the average of three independent experiments. * indicates statistical comparison. p < .005. (E) Quantitation of the number of GFP-Atg8 autophagosomes present in WT and ydj1-151 cells incubated in glucose or galactose medium (n = 100 cells). Quantities indicate the average of three independent experiments. All images in this figure were acquired using the EVOS microscope.

https://doi.org/10.1371/journal.pone.0191490.g007

Discussion

The accumulation of misfolded proteins in neurons is a hallmark of neurodegenerative diseases [60, 61]. The stressful environment caused by protein aggregates may interfere with cellular functions. The chaperone network prevents aggregation and facilitates refolding [5]. Thus, chaperone expression alterations are associated with aging and neurodegeneration [53]. The role of chaperones in protein folding, disaggregation and proteasome-dependent degradation has been well documented [5, 12], but the role of chaperone proteins in autophagy-dependent clearance of misfolded proteins is not fully understood. Using yeast as a model system, we found that cytosolic Hsp70 proteins and their co-factors, Hsp40 co-chaperone and NEFs, are required for the efficient clearance of mutated Htt103QP through autophagy. Moreover, this defect is likely due to the failure of IB formation, as evidenced by the accelerated protein aggregation and the increased number of aggregates in the mutant cells.

The rapid accumulation of Htt103QP aggregates in sse1Δ, fes1Δ, and ydj1-151 mutant cells is unlikely attributable to impaired Htt103QP degradation by the proteasome. All tested chaperone mutants exhibited proteasome-dependent degradation, although we found slight delay in some strains. Since polyubiquitination is a targeting signal for proteasome-dependent degradation and mutant Huntingtin protein is known to be ubiquitinated [27, 62, 63], we examined the ubiquitination state of Htt103QP in sse1Δ cells. The ubiquitination of Htt103QP was unaffected by sse1Δ deletion, arguing against the role of Sse1 in Htt103QP ubiquitination. We further tested the possibility that Sse1 and Fes1 recruit disaggregase Hsp104 to Htt103QP aggregates [64], but we found clear co-localization of Hsp104 with Htt103QP in sse1Δ and fes1Δ mutants. One explanation is that the Hsp104 is not fully functional in these mutant cells even though its recruitment to Htt103QP aggregates is normal. Alternatively, Sse1, Fes1, and Ydj1 chaperones could have a disaggregase function independent of Hsp104.

We have previously shown that Htt103QP can be efficiently cleared via autophagy [26]. We found that Sse1, Fes1, and Ydj1 are all required for efficient autophagy of Htt103QP. The rate of clearance of the multiple aggregates observed in these mutants is significantly delayed compared to WT cells. However, Htt103QP is still observed in the vacuole in some mutant cells, indicating autophagic degradation of Htt103Qp is not abolished completely. We found a stark decrease in the colocalization of autophagosmal marker Atg8 with Htt103QP aggregates in all three mutants. It is possible that the biochemical nature of the aggregates in these mutants differs from the IB in WT cells, and thus cannot be efficiently recognized and encapsulated into autophagosomes [65]. Another possibility is that the accelerated Htt103QP aggregation may block the binding sites for an autophagy receptor. Recent studies suggest that the ubiquitin-Atg8 adaptor Cue5 acts as the receptor to facilitate autophagy-dependent clearance of mutated Huntingtin [63]. Further studies are needed to examine the binding of Htt103QP to Cue5 in cells lacking HSPs.

It is of interest that the IB formation defects found in yeast cells lacking Sse1 and Fes1 do not cause cytotoxicity like in other mutants, such as dsk2Δ and cdc48-1 and bmh1Δ [25, 26, 66]. One explanation is that the aggregates formed in sse1Δ and fes1Δ mutants are less toxic [67, 68]. However, the aberrant aggregates in sse1Δ mutants appear morphologically similar to the aggregates in cells overexpressing Htt103Q that lacks the proline-rich domain, and yeast cells are very sensitive to Htt103Q expression [38]. Our explanation is that sse1Δ mutant cells accumulate multiple aggregates even in the absence of Htt103QP expression as indicated in Fig 4, which may contribute to the slow growth of sse1Δ cells. Htt103QP expression does not further exacerbate the growth defect in sse1Δ cells.

Chaperone proteins are highly conserved from yeast to human. Our findings suggest that a chaperone network regulates aggregation of mutated Huntingtin. On one hand, these chaperone proteins prevent aggregation of misfolded proteins after their synthesis. On the other hand, this network is required for IB formation and the subsequent autophagic clearance. Therefore, functional HSPs are critical for the healthy cellular environment, and dysfunctional HSPs may contribute to disease development. In line with this, transgenic expression of Hsp110 is linked to extended survival in mice with ALS [69]. In contrast, loss of Hsp110 is linked to early accumulation of Alzheimer’s disease proteins Aβ and hyperphosphorylated Tau in mice [70]. Additionally, Hsp70 is known to bind and facilitate refolding of mutant Huntingtin, α-synuclein, Aβ, hyperphosphorylated Tau and mutant SOD1 [22]. Further mechanistic insights into this pathway may uncover potential therapeutic strategies.

Materials and methods

Strains, plasmids and growth conditions

Yeast strain used in this study are W303 unless otherwise noted. Genotypes are listed in S1 Table. Gene deletions and GFP tagging of HSP104 were performed using a PCR-based method [71]. The plasmid of Flag- and GFP-tagged Htt103QP fragment with galactose-inducible promoter (PGALFlag-Htt103QP-GFP) was originally from the Lindquist lab [23]. Using this plasmid, we subcloned the PGALFlag-Htt103QP-GFP into pRS406 vector, and the resulting plasmid was then inserted into the yeast genome after EcoRV digestion. To construct the PGALFlag-Htt103QP-mApple plasmid, the GFP was replaced with mApple using PCR-based subcloning. The pRS414-GFP-Atg8 plasmid, originally from the Klionsky lab [72], was obtained from Addgene. The p1217 plasmid is a derivative of pRS414 containing a galactose inducible promoter followed by 3xHA. We used PCR-based protocol to subclone the WT ubiquitin sequence from pRK5-HA-Ub [73] into p1217 to obtain PGALHA-Ub plasmid. Yeast extract/peptone medium supplied with raffinose, galactose or glucose was used for the growth of yeast strains, except for those carrying centromeric plasmids.

Fluorescence imaging

Confocal fluorescence imaging was performed using a Zeiss LSM 880 microscope (Oberkocken, Germany). All other fluorescence imaging analyses were performed using an EVOS microscope (Thermo Fisher Scientific, Waltham, MA). For all imaging analysis, samples were fixed in paraformaldehyde for 5 minutes then resuspended in 1× PBS buffer.

Western blotting

Protein samples were prepared using an alkaline method and resolved by 10% SDS–PAGE. Anti-Flag antibody was purchased from Sigma-Aldrich (St. Louis, MO); anti-GFP antibody was from Santa Cruz Biotechnology (Santa Cruz, CA); anti-Pgk1 antibody was from Molecular Probes (Eugene, OR); anti-HA antibody was from Covance Research Products (San Diego, CA). The horseradish peroxidase–conjugated goat anti-mouse IgG secondary antibody was from Santa Cruz Biotechnology (Santa Cruz, CA).

Starvation induced GFP-Atg8 cleavage assay

Cells containing a pRS414-GFP-ATG8 plasmid were grown in synthetic glucose medium lacking tryptophan (SC—TRP) to OD600 1.0, then shifted to nitrogen deficient medium (SD-N) for 24 hr. SD-N medium contains YNB, glucose and water.

Ubiquitination assay

Cells containing PGALFlag-Htt103QP-GFP with either p1217 empty vector or PGALHA-Ub plasmid were grown in raffinose containing synthetic medium without tryptophan (SC—TRP) to OD600 = 0.2. Galactose was then added to the medium for 4 hr to induce expression of HA-Ub and Flag-Htt103QP-GFP. Cells were then harvested by centrifugation at 10,000 rpm for 10 min at 4°C. Cells were washed once with water and then resuspended in RIPA buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 5mM EDTA, 0.05% Tween-20) along with azide and protease inhibitor cocktail (EMD Millipore Corp., Billerica, MA). Samples were then frozen by slowly dripping them into liquid nitrogen to form pellets. Pellets were crushed using a freezer mill. Once crushed, samples were allowed to thaw on ice in the presence of 20mM deubiquitinase inhibitor NEM (Sigma Aldrich). Then, samples were centrifuged at 4,000 rpm for 20 min at 4°C. Supernatant was collected and centrifuged again at 20,000 g for 20 min at 4°C. Input samples were saved, then M2 Flag agarose beads (Sigma Aldrich) were used to immunoprecipitate Flag-Htt103QP-GFP. The beads were washed 3 times and resuspended in 1× SDS loading buffer and boiled for 5 min. Western blotting was performed using anti-Flag, anti-HA and anti-Pgk1 antibodies.

Sedimentation assay

The protocol was described previously with minor adaptation for our experiments [46]. Cells were grown in YEP (yeast extract peptone) medium containing 2% raffinose. Once cells were in mid-log phase, galactose was added to 2% and the cells were incubated at 30°C for 2 hrs. Five mL of cells were harvested and resuspended in 100μL lysis buffer (100mM Tris-HCl pH 7.5, 200mM NaCl, 1mM EDTA, 1mM DTT, 5% glycerol and 0.1% Nonidet P40) plus PMSF with 100μL 0.5mm acid-washed glass beads. Cells were lysed using a beads beater at 4°C. Cell debris were cleared by centrifuging lysates at 700g for 1 min at 4°C. We removed 50μL lysate, representing total lysate, and added to 50μL SUMEB (8M Urea, 1% SDS, 10mM MOPS pH 6.8, 10mM EDTA, 1mM PMSF, 0.01% Bromophenol Blue). The remaining lysate (100μL) was centrifuged at 12,800 g for 15 min at 4°C. We added 100μL supernatant, representing the soluble fraction, to 100μL SUMEB. The pellet representing the insoluble fraction was resuspended in 100μL lysis buffer and 100μL SUMEB. All samples were incubated at 65°C for 10 minutes and then centrifuged for 5 minutes at 12,800g. Proteins were resolved on SDS-PAGE gels, transferred to nitrocellulose membrane and immunoblotted with anti-Flag (Sigma) and anti-Pgk1 (Molecular Probes) antibodies.

SDS agarose gel electrophoresis (SDS-AGE)

We adapted the protocol from previous work with some modification [31]. Briefly, yeast cells were grown in 10 mL non-inducible raffinose medium until mid-log phase, then galactose was added to 2% for 3 hrs. 1mL of cells was harvested and resuspended in RIPA (0.05% Tween, 5mM EDTA, 50mM Tris-HCl pH 7.5, 150mM NaCl) buffer plus protease inhibitor cocktail (Millipore Calbiochem) and PMSF. Cells were broken using a beads beater. Cell debris were cleared by centrifuging at 300 g for 3 min. Sample buffer (150mM Tris, pH 6.8, 33% glycerol, 1.2% SDS, bromophenol blue) was added at equal volume to lysate and samples were incubated at 37°C for 15 min. Samples were then loaded on a 1% agarose gel with running buffer (25mM Tris-HCl, 192mM glycine, and 0.1% SDS). Gels were run at 125V until blue dye reached the bottom. Gel was then semi-dry transferred onto a nitrocellulose membrane for 30 min at constant 0.09 Amps at 4°C. The membrane was probed with anti-Flag antibody (Sigma).

Statistical analysis

Statistical analysis and significance was performed using student T-test. All error bars shown in this manuscript represent standard error of the mean (SEM). ImageJ was used for quantitation of Western blot images.

Supporting information

S1 Table. Yeast strains used in this study.

https://doi.org/10.1371/journal.pone.0191490.s001

(DOCX)

S1 Fig. Htt103QP inclusion body formation and degradation in chaperone mutants.

(A) Fluorescent images of Htt103QP-GFP in WT, ssa1Δ, ssa2Δ, ssa3Δ, and ssa4Δ cells after 16 hr induction in galactose medium. Cells were quantified for the number of aggregates in each cell: 1, 2 or > 2 (n = 100 cells). The results are the average of three independent experiments. Scale bar = 5μm. (B) Htt103QP degradation in cytosolic Hsp70 mutants. WT, ssa1Δ, ssa2Δ, ssa3Δ, and ssa4Δ cells with PGALFlag-Htt103QP-GFP were grown in non-inducible raffinose containing medium to mid-log phase. Galactose was then added for 1 hr to induce Htt103QP overexpression. Glucose was subsequently added to shut off expression. Cells were collected at indicated time points to determine Htt103QP protein levels. Pgk1: loading control. (C) Fluorescent images of Htt103QP-GFP in WT, hsp42Δ, hsp82Δ, hsc82Δ, sti1Δ, and ssb1Δ cells after 16 hr induction. Cells were quantified for the number of aggregates in each cell: 1, 2 or > 2 (n = 100 cells). The results are the average of three independent experiments. Scale bar = 5μm. (D). Htt103QP degradation in WT, hsp42Δ, hsp82Δ, hsc82Δ, sti1Δ, and ssb1Δ cells. Same protocol was used as in (B). All microscopy in this figure was performed on the EVOS microscope.

https://doi.org/10.1371/journal.pone.0191490.s002

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S2 Fig. Hsp104-GFP localization in WT yeast cells.

WT cells containing HSP104-GFP were grown in either YPD, YEP+Galactose (YEPG), or YEP+Raffinose (YEPR) for at 30°C for 3 hrs. Cells were then collected, fixed with 70% ethanol, stained with DAPI, and visualized using microscopy. DAPI staining was used to mark the nucleus. Representative GFP and DAPI images are shown. Scale bar = 5μm.

https://doi.org/10.1371/journal.pone.0191490.s003

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S3 Fig. Proteasome-dependent Htt103QP degradation in HSP mutants.

(A) Proteasome inhibitor MG132 blocks Htt103QP degradation after short-time induction. WT cells growing at 30°C in non-inducible (YEP + raffinose) medium containing 0.1% L-proline were treated with 0.003% SDS for 3 hr. Then, either DMSO or 75μM MG132 was added to the cultures for 30 min. Galactose was then added to induce Htt103QP overexpression for 1 hr. Finally, glucose was added to shut off Htt103QP expression. Cells were collected when in galactose (Gal) and also at 90 and 180 min after glucose addition. The Htt103QP protein levels were detected using anti-Flag antibody. Pgk1: loading control. The quantitative degradation kinetics expressed as percent remaining is shown in the right panel. (B) Htt103QP degradation in cells lacking autophagy genes after short-time induction. WT, atg8Δ, and atg7Δ were grown in non-inducible YEP + raffinose medium to mid log phase, then galactose was added for 1 hr to induce Htt103QP overexpression. Glucose was then added to shut off Htt103QP expression. Samples were collected after 1 hr galactose induction and after glucose addition for 90 and 180 min. The Htt103QP protein levels were detected using anti-Flag antibody. Pgk1: loading control. (C) Htt103QP degradation in WT and sse1Δ mutant. Same protocol was used as in (B). The quantitative degradation kinetics expressed at percent remaining is shown in the right panel. (D) Htt103QP degradation in WT, sse2Δ, snl1Δ and fes1Δ cells. Same protocol was used as described in (B). The quantitative degradation kinetics expressed at percent remaining is shown in the right panel. (E) Htt103QP degradation in WT and ydj1-151 cells. Cells were grown at 25°C in non-inducible YEP + raffinose medium to mid log phase, then galactose was added to induce Htt103QP overexpression for 50 min. Cells were then shifted to 37°C for 10 min before glucose was added. Samples were taken after 1 hr galactose induction (Gal) and after glucose addition for 90 and 180 min. The quantitative Htt103QP degradation is shown in the right panel.

https://doi.org/10.1371/journal.pone.0191490.s004

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S4 Fig. The solubility and ubiquitination of Htt103QP in WT and sse1 mutant cells.

(A) Htt103QP sedimentation assay in WT and sse1Δ cells. Cells were grown in non-inducible raffinose medium at 30°C to mid-log phase, then galactose was added to 2% for 2 hr. Cells were lysed with beads beater and Htt103QP was fractionated into soluble (S) and insoluble (I) fractions by centrifugation. The preparation of the T (total), S and I fractions was described in the Materials and Methods section. Anti-Flag antibody was used to detect Htt103QP, and anti-Pgk1 antibody was used to determine the distribution of Pgk1 in each fraction. (B) Htt103QP ubiquitination in WT and sse1Δ cells. WT and sse1Δ cells carrying PGALFlag-Htt103QP-GFP and PGALHA-Ub or PGAL-HA were grown in raffinose containing medium to early log phase. Galactose was added to induce Htt103QP and Ub overexpression for 4 hr. Flag-Htt103QP-GFP was immunoprecipitated (IP) using anti-Flag M2 agarose beads. Anti-Flag antibody was used to detect Htt103QP protein level. Anti-HA antibody was used to detect Ub protein level. Pgk1: loading control.

https://doi.org/10.1371/journal.pone.0191490.s005

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Acknowledgments

We thank the yeast community at Florida State University for comments and suggestions. We sincerely thank Dr. Jeff Brodsky and Dr. Robert Tomko Jr for strains and plasmids. We are grateful to Dr. Akash Gunjan for his assistance in the ubiquitination assay. We thank Ruth Didier for her assistance with confocal imaging. We thank the Florida State University Biology Core Facility for help with plasmid constructions.

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