Figures
Incorrect versions of Figs 3, 4 and 6 are included in the published paper.
In Fig 3B and 3D, the Y-axis scale should be adjusted for clarity. Please see the corrected Fig 3 here.
Caco-2/TC7 cells grown for 21 days were incubated with 2.5, 25 and 75 mM D-Glucose or D-Fructose for 3hrs, washed in sugar free KBS, followed by 10 mins exposure to (a and c) [14 C] 10 mM D-Glucose and (b and d) [14C] 10 mM D-Fructose. Cellular uptake of D-Glucose and D-Fructose was measured by radioactive scintillation spectrometry. Osmolarity was adjusted by adding Mannitol. Uptake is corrected for simple diffusion of 10 mM [14C] L-Glucose. Data are expressed as nMoles D-Glucose or D-Fructose uptake/well/10min ± SD of n = 4 per condition. *P < 0.05, **P < 0.01, ***P < 0.001, ns = not significant.
In Fig 4B, 10 mM AceK+Lact is not significant when compared to 10 mM AceK alone. In the published Fig 4B, the significance (*) is an incorrect comparison between 10 mM AceK+Lact and the control. Please see the corrected Fig 4 here.
(a) Caco-2/TC7 cells grown for 21 days were incubated with 2.5, 25 and 75 mM D-Glucose for 3hrs in the presence or absence of 0.5 mM lactisole, washed three times with glucose free KBS, followed by 10 min exposure to [14 C] 10 mM D-Glucose. Osmolarity was adjusted by adding Mannitol. (b) Cells were incubated with 75 mM D-Glucose in the presence of 10 mM AcesulfameK (AceK) or Sucralose with the addition of lactisole at 0.5mM. Osmolarity was adjusted for by adding Mannitol. Cellular uptake of D-Glucose was measured by radioactive scintillation spectrometry. Uptake is corrected for simple diffusion of 10 mM [14C] L-Glucose. Data are expressed as nMoles D-Glucose uptake/well/10min ± SD of n = 4 per condition. ns = not significant.
In Fig 6C and 6D, the x-axis is labelled as HFCS but should be labeled as D-Glu + D-Fru. The y-axis scale should also be adjusted for clarity. Please see the corrected Fig 6 here.
Caco-2/TC7 cells grown for 21 days were incubated with 2.5 and 75 mM Sucrose or a 50/50 mix of free D- Glucose/D-Fructose in the presence or absence of 0.5 mM lactisole for 3hrs, washed in sugar free KBS. (a) and (c) are cellular uptake of [14C] 10 mM D-Glucose for 10 mins (b) and (d) are cellular uptake of [14C] 10 mM D-Fructose measured by radioactive scintillation spectrometry. Osmolarity was adjusted by adding Mannitol. Uptake is corrected for simple diffusion of 10 mM [14C] L-Glucose. Data are expressed as nMoles sugar uptake/well/10min ± SD of n = 4 per condition. *P < 0.05, **P < 0.01, ***P < 0.001, ns = not significant.
Reference
Citation: O’Brien P, Corpe CP (2017) Correction: Acute Effects of Sugars and Artificial Sweeteners on Small Intestinal Sugar Transport: A Study Using CaCo-2 Cells As an In Vitro Model of the Human Enterocyte. PLoS ONE 12(10): e0186016. https://doi.org/10.1371/journal.pone.0186016
Published: October 3, 2017
Copyright: © 2017 O’Brien, Corpe. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.