Figures
In Figs 2, 3, 4 and 5, and S1, S4, S5, S7, S9, S10, S11, S12, S13, S14, S16, S17, S18, S19 and S20 Figs, the y-axes are incorrectly labeled “pmol/min/mg protein”, when they should be labeled “nmol/min/mg protein”. Please see the corrected files here.
Statistical significance was analyzed via a one way ANOVA (main effect of strain, P<0.0001) (n = 31–45). Asterisks (*) denote statistical significance. Bars ± SEM.
(A) Treatment with FCCP caused a significant increase in OCR in all strains (two way ANOVA, main effects of strain, treatment and their interaction, P<0.0001 for all); however, L4 fzo-1, drp-1 and isp-1 had reduced maximal respiratory capacity compared to N2 nematodes (Student’s t-test, p = 0.03, p<0.0001, p = 0.01, respectively). (B) Spare respiratory capacity was reduced in fzo-1 and drp-1, compared to wild-type nematodes (one way ANOVA, P = 0.022). (n = 12–20). Asterisks (*) denote statistical significance. Bars ± SEM.
(A) Sodium azide and DCCD caused significantly different reductions in OCR in N2, drp-1, pink-1, and pdr-1 nematodes (two way ANOVA, main effects of strain (P<0.0001), treatment (P<0.0001) and their interaction (p = 0.0002)), while fzo-1 and isp-1 responses were not significantly different. (B) L4 fzo-1 and isp-1 nematodes have reduce proton leak, while pink-1 C. elegans have increased leak (one way ANOVA, P<0.0001). (n = 8–12). Asterisks (*) denote statistical significance. Bars ± SEM.
Supporting Information
S1 Fig. Response of L4 N2 nematodes to sodium azide alone and post-FCCP.
Response to sodium azide was assessed statistically with a one way ANOVA (P = 0.0005). Asterisks (*) denote statistical significance. Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s001
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S4 Fig. Titration of Dicyclohexylcarbodiimide in L4 N2 nematodes.
Significance assessed with a one way ANOVA (P<0.0001), followed by student’s T-tests for pairwise comparisons. Asterisks (*) denote statistical significance. Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s002
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S5 Fig. Effect of DMSO concentration of efficacy of 20μM DCCD.
Significance assessed with a one way ANOVA (P<0.0001), followed by student’s T-tests for pairwise comparisons. Asterisks (*) denote statistical significance. Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s003
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S7 Fig. Titration of FCCP in L4 N2 nematodes.
Significance assessed with a one way ANOVA (main effect of treatment, P<0.0001). Asterisks (*) denote statistical significance. Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s004
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S9 Fig. Titration of sodium azide in L4 N2 nematodes.
Significance assessed with a one way ANOVA (main effect of treatment, P<0.0001). Asterisks (*) denote statistical significance. Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s005
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S10 Fig. Basal OCR is elevated in drp-1 and reduced in isp-1 L4 C. elegans on a per nematode basis.
Asterisks (*) denote statistical significance. Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s006
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S11 Fig. ATP coupled respiration.
(A) 20μM DCCD caused a significant reduction in OCR in all strains (two way ANOVA, main effects of strain and treatment, P<0.0001 for both, but not their interaction). (B) A trend in increased ATP coupled respiration was observed in fzo-1 nematodes (one way ANOVA, P = 0.07). (n = 12–16). Asterisks (*) denote statistical significance. Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s007
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S12 Fig. ATP coupled respiration per nematode.
(A) 20μM DCCD caused a significant reduction in OCR in all strains (two way ANOVA, main effects of strain and treatment, P<0.0001 for both, but not their interaction), (B) but no significant effect on ATP-linked respiration was observed. Asterisks (*) denote statistical significance. Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s008
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S13 Fig. Maximal and spare respiratory capacity in L4 C. elegans on a per nematode basis.
(A) fzo-1, isp-1, and drp-1 nematodes have a significantly reduced FCCP response (two way ANOVA, main effects of strain, treatment, and their interaction, P = 0.0001 for all) (A) and (B) spare respiratory capacity. Asterisks (*) denote statistical significance. Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s009
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S14 Fig. Proton leak per L4 nematode.
(A) Effect of sodium azide and DCCD on OCR (two way ANOVA, P>0.05) and (B) proton leak per nematode measured (two way ANOVA, P>0.05).
https://doi.org/10.1371/journal.pone.0168738.s010
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S16 Fig. Proton leak per L4 nematode.
DMSO had no effect on OCR in any of the strains tested (two way ANOVA, P>0.05). Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s011
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S17 Fig. Basal OCR and sodium azide response.
Sodium azide caused a significant reduction in OCR in all strains tested (one was ANOVA, P<0.0001). Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s012
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S18 Fig. Oligomycin pre-incubation with bus-8-deficient nematodes.
A 12 hour pre-incubation with oligomycin caused a significant reduction in OCR (one way ANOVA, P<0.0001). Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s013
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S19 Fig. Oligomycin titration with bus-8-deficient nematodes.
Treatment with oligomycin caused a significant reduction in OCR (one way ANOVA, P = 0.0007). Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s014
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S20 Fig. DCCD titration with bus-8-deficient nematodes.
Treatment with DCCD caused a significant reduction in OCR (one way ANOVA, P<0.0001). Bars ± SEM.
https://doi.org/10.1371/journal.pone.0168738.s015
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Reference
- 1. Luz AL, Rooney JP, Kubik LL, Gonzalez CP, Song DH, Meyer JN (2015) Mitochondrial Morphology and Fundamental Parameters of the Mitochondrial Respiratory Chain Are Altered in Caenorhabditis elegans Strains Deficient in Mitochondrial Dynamics and Homeostasis Processes. PLoS ONE 10(6): e0130940. pmid:26106885
Citation: Luz AL, Rooney JP, Kubik LL, Gonzalez CP, Song DH, Meyer JN (2016) Correction: Mitochondrial Morphology and Fundamental Parameters of the Mitochondrial Respiratory Chain Are Altered in Caenorhabditis elegans Strains Deficient in Mitochondrial Dynamics and Homeostasis Processes. PLoS ONE 11(12): e0168738. https://doi.org/10.1371/journal.pone.0168738
Published: December 15, 2016
Copyright: © 2016 Luz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.