Figures
The authors would like to correct Fig 5, as errors were introduced in the preparation of this figure for publication. In the middle panel of Fig 5C, the loading control blot was mistakenly duplicated as well as mislabeled β-actin instead of GAPDH. The authors have provided a corrected version of Fig 5 here that includes both a corrected blot and a corrected label. The authors confirm that these changes do not alter their findings. The authors have provided the underlying images as Supporting Information.
In all, 1×106 cells were incubated with 100 μg/ml of C1 for 3 hours and (Ai) intra-mitochondrial O2- was determined using the fluorescent dye MitoSox™ RED Mitochondrial O2- Indicator and intracellular H2O2 was detected by DCHF-DA loading and analyzed by flow cytometry. (Ai) Cells were pre-incubated with catalase (7000 units/ml) or NAC (200 μM) for 1 hour before treatment with C1 (100 μg/ml for 3 hours) and intracellular H2O2 was determined. (Aii) Cells were transiently transfected with 8 μg of pCINeoEV or pCINeo+CAT for 48 hours (Aiii) and treated with C1 (100 μg/ml for 3 hours) and intracellular H2O2 was determined (Aii). Cells were pre-incubated with catalase (7000 units/ml for 1 hour) or were transiently transfected with pCINeoEV or pCINeo+CAT before exposure to C1 (100 μg/ml for 24 hours), and total cell lysates were immunoblotted for (B) PARP cleavage and (C) LC3II accumulation.
Reference
Citation: Wong CH, Iskandar KB, Yadav SK, Hirpara JL, Loh T, Pervaiz S (2016) Correction: Simultaneous Induction of Non-Canonical Autophagy and Apoptosis in Cancer Cells by ROS-Dependent ERK and JNK Activation. PLoS ONE 11(7): e0159352. https://doi.org/10.1371/journal.pone.0159352
Published: July 8, 2016
Copyright: © 2016 Wong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.