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Open Access

Correction

Correction: Adipose Tissue-Derived Mesenchymal Stem Cells in Long-Term Dialysis Patients Display Downregulation of PCAF Expression and Poor Angiogenesis Activation

  • Shuichiro Yamanaka,
  • Shinya Yokote,
  • Akifumi Yamada,
  • Yuichi Katsuoka,
  • Luna Izuhara,
  • Yohta Shimada,
  • Nobuo Omura,
  • Hirotaka James Okano,
  • Takao Ohki,
  • Takashi Yokoo

There is an error in Fig 1. Panel B is a duplicate of Panel A. Please see the corrected Fig 1 below.

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Fig 1. Characteristics of mesenchymal stem cells from healthy controls (HC-MSCs) and patients with ESKD (KD-MSCs).

(A) Representative images of HC-MSCs (left) and KD-MSCs (right; original magnification, ×100). (B) Flow cytometric analysis of cell surface marker expression of HC-MSCs (solid lines; n = 6) and KD-MSCs (dashed lines; n = 9). Isotype-matched IgG controls are represented by solid histograms. (C) Comparison of cell surface marker expression in HC-MSCs (n = 6) and KD-MSCs (n = 9). The percentages of positive cells are shown. Data are the mean ± SE. There were no significant differences.

https://doi.org/10.1371/journal.pone.0157282.g001

There are a number of errors in the caption for Fig 5, “Western blot analysis of PCAF, HIF-1α, and VEGF expression under hypoxia and normoxia.’ Please see the complete, correct Fig 5 caption here.

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Fig 5. Western blot analysis of PCAF, HIF-1α, and VEGF expression under hypoxia and normoxia.

(A) Western blot analysis of PCAF expression in KD-MSCs (n = 9) and HC-MSCs (n = 6) under normoxia and hypoxia (1% O2). Data are the mean ± SE. **P < 0.01 normoxia versus hypoxia in HC-MSCs (two-tailed, unpaired t-test). (B) Western blot analysis of PCAF expression at 24 h under hypoxia showed it to be clearly upregulated in HC-MSCs. There was no change in PCAF in KD-MSCs under hypoxia. Data are the mean ± SE (HC-MSCs n = 6, KD-MSCs n = 9; *P<0.05 versus normoxia, two-tailed, paired t-test). (C) Western blot analysis of HIF-1α expression in KD-MSCs (n = 9) and HC-MSCs (n = 6) under normoxia and hypoxia. Data are the mean ± SE. *P<0.05 versus normoxia (two-tailed, unpaired t-test). (D) Western blot analysis of VEGF expression in KD-MSCs (n = 9) and HC-MSCs (n = 6) under normoxia and hypoxia. Data are the mean ± SE. *P<0.05 versus normoxia (two-tailed, unpaired t-test). (A–D) MSC lines were isolated independently. Because of the differing molecular weights of PCAF, HIF1α, VEGF, and β-actin, molecular-weight-bands in the western blot were cut out and stained with their respective specific antibodies. The endogenous β-actin band was used as an internal control for each band derived from other proteins.

https://doi.org/10.1371/journal.pone.0157282.g002

Reference

  1. 1. Yamanaka S, Yokote S, Yamada A, Katsuoka Y, Izuhara L, Shimada Y, et al. (2014) Adipose Tissue-Derived Mesenchymal Stem Cells in Long-Term Dialysis Patients Display Downregulation of PCAF Expression and Poor Angiogenesis Activation. PLoS ONE 9(7): e102311. pmid:25025381

Citation: Yamanaka S, Yokote S, Yamada A, Katsuoka Y, Izuhara L, Shimada Y, et al. (2016) Correction: Adipose Tissue-Derived Mesenchymal Stem Cells in Long-Term Dialysis Patients Display Downregulation of PCAF Expression and Poor Angiogenesis Activation. PLoS ONE 11(6): e0157282. https://doi.org/10.1371/journal.pone.0157282

Published: June 6, 2016

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