Porphyromonas gingivalis Gingipain-Dependently Enhances IL-33 Production in Human Gingival Epithelial Cells

Hiroyuki Tada; Takashi Matsuyama; Takashi Nishioka; Makoto Hagiwara; Yusuke Kiyoura; Hidetoshi Shimauchi; Kenji Matsushita

doi:10.1371/journal.pone.0152794

Abstract

The cytokine IL-33 is constitutively expressed in epithelial cells and it augments Th2 cytokine-mediated inflammatory responses by regulating innate immune cells. We aimed to determine the role of the periodontal pathogen, Porphyromonas gingivalis, in the enhanced expression of IL-33 in human gingival epithelial cells. We detected IL-33 in inflamed gingival epithelium from patients with chronic periodontitis, and found that P. gingivalis increased IL-33 expression in the cytoplasm of human gingival epithelial cells in vitro. In contrast, lipopolysaccharide, lipopeptide, and fimbriae derived from P. gingivalis did not increase IL-33 expression. Specific inhibitors of P. gingivalis proteases (gingipains) suppressed IL-33 mRNA induction by P. gingivalis and the P. gingivalis gingipain-null mutant KDP136 did not induce IL-33 expression. A small interfering RNA for protease-activated receptor-2 (PAR-2) as well as inhibitors of phospholipase C, p38 and NF-κB inhibited the expression of IL-33 induced by P. gingivalis. These results indicate that the PAR-2/IL-33 axis is promoted by P. gingivalis infection in human gingival epithelial cells through a gingipain-dependent mechanism.

Citation: Tada H, Matsuyama T, Nishioka T, Hagiwara M, Kiyoura Y, Shimauchi H, et al. (2016) Porphyromonas gingivalis Gingipain-Dependently Enhances IL-33 Production in Human Gingival Epithelial Cells. PLoS ONE 11(4): e0152794. https://doi.org/10.1371/journal.pone.0152794

Editor: Motohiro Komaki, Tokyo Medical and Dental University, JAPAN

Received: December 16, 2015; Accepted: March 19, 2016; Published: April 8, 2016

Copyright: © 2016 Tada et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the paper and its Supporting Information files.

Funding: This work was supported by Grand-in-Aids for Scientific Research (25463225 to HT, 22390354 to KM) from the Japan Society for the Promotion of Science; and the Takeda Science Foundation (HT). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Introduction

Epithelial cells play a central role in initiating the innate immune response to pathogens in mucosal tissues, including the oral mucosa. Interleukin (IL)-33 belongs to the IL-1 cytokine family, and it is constitutively expressed in the nuclei of non-immune cells such as fibroblasts, adipocytes, epithelial cells, endothelial cells, and smooth muscle cells, and in some immune cells such as monocytes and dendritic cells [1, 2]. Epithelial cell-derived IL-33 augments Th2 cytokine-mediated inflammation in response to bacterial components [3, 4]. Toll-like receptor ligands and proinflammatory stimuli can up-regulate IL-33 expression [5–8]. Various types of immune cells such as basophils, eosinophils, Th2 cells, mast cells, NKT cells, NK cells, and type 2 innate lymphoid cells (ILC2) express the IL-33 receptor ST2 [9]. Interleukin-33 helps to promote host defense against parasites or bacteria towards Th2 cytokine-associated inflammation [10–14]. In contrast, circumstantial evidence suggests that IL-33 is also involved in the development of inflammatory responses. Interleukin-33 expression is increased in epithelial cells of mucosal lesions arising due to chronic inflammatory diseases such as allergic rhinitis, chronic obstructive lung disease, and chronic colitis [15–18]. Interleukin-33 can control inflammatory responses either positively or negatively. Type 2 innate lymphoid cells produce IL-5 and IL-13 in response to IL-33 and subsequently induce Th2-type inflammation [19–21]. Furthermore, mast cells secrete chemokines in response to IL-33 and subsequently induce neutrophil migration [22]. These activities suggest that IL-33 exerts proinflammatory effects in various chronic inflammatory diseases. However, whether IL-33 is induced in gingival epithelial cells during the development of periodontal disease remains unclear.

Porphyromonas gingivalis is a primary pathogen that is involved in chronic periodontitis and it has a diversity of virulence factors that manipulate immune responses, resulting in chronic inflammation and bone loss [23]. This bacterium synthesizes two classes of cysteine proteases; arginine-specific gingipains (RgpA and RgpB) and lysine-specific gingipain (Kgp), which constitutes a major virulence factor [24]. Gingipains are localized in soluble and cell-associated forms, and are secreted as outer membrane blebs [25, 26]. Gingival epithelial cells comprise part of the first line of innate immune responses against P. gingivalis infection in periodontal tissue. Chronic inflammation results when P. gingivalis invades gingival epithelial cells [27]. We recently discussed a possible role of IL-33 in the pathogenesis of chronic periodontitis [28]. Although gingival tissues from patients with chronic periodontitis express IL-33 [29], whether or not P. gingivalis increases IL-33 expression in gingival epithelial cells remains unknown. The present study found that P. gingivalis upregulates IL-33 expression in human gingival/oral epithelial cells via endogenous gingipain-dependent mechanisms.

Materials and Methods

Ethics statement

Gingival tissues were derived from the junctional epithelium of teeth that required extraction from patients (n = 5) with chronic periodontitis at the time of initial examination and from patients (n = 5) without periodontal diseases. The Ethics Committee at Kagoshima University Medical and Dental Hospital reviewed and approved the study protocol (No. 24–143) and all patients provided written, informed consent to the extraction of their teeth and the use of their periodontal tissues for research.

Reagents

Porphyromonas gingivalis fimbriae and PGTP2-RL were a gift from Dr. T. Ogawa (Asahi University). The properties of P. gingivalis fimbriae [30–31] and PGTP2-RL [32] have been described in detail. The fimbriae were purified from the P. gingivalis 381 (type I fim A) strain prepared by Dr. Ogawa, and LPS containing heterogeneous lipid A structures including tetra- and penta-acylated monophosphorylated lipid A from P. gingivalis prepared as described by Darveau et al. (2004) [33] was purchased from InvivoGen (San Diego, CA). SB203580, cycloheximide and cytochalasin D were obtained from Calbiochem-Novabiochem Co. (La Jolla, CA). U-73122, GF109203X, ammonium pyrrolidine dithiocarbamate (PDTC) and dimethyl sulfoxide (DMSO) were sourced from Sigma-Aldrich (St. Louis, MO). Phe-Pro-Arg-chloromethyl ketone (FPR-cmk) was obtained from Enzo Life Sciences (Farmingdale, NY) and KYT-36 was sourced from the Peptide Institute (Osaka, Japan).

Culture of P. gingivalis and preparation of whole cell preparations

Porphyromonas gingivalis W83, ATCC 33277, KDP131 (ΔrgpA), KDP132 (ΔrgpB), KDP129 (Δkgp), KDP133 (ΔrgpA ΔrgpB) and KDP 136 (Δkgp ΔrgpA ΔrgpB) [34] were cultured anaerobically (10% CO₂, 10% H₂, and 80% N₂) at 37°C for 60 h in enriched tryptic soy broth containing 1% tryptone (Difco, Detroit, MI), 3% tryptic soy (Becton Dickinson Co., Franklin Lakes, NJ), 2.5% yeast extract (Becton Dickinson), 0.1% _L-cysteine (Wako Pure Chemical Industries, Osaka, Japan), 5 μg/mL of hemin, and 5 μg/mL of menadione. After three washes with PBS, whole bacterial cells at the log-phase of growth were resuspended in PBS, lyophilized by centrifugation in vacuo and then resuspended in distilled water. Viability monitored by counting colony-forming units (CFU) in blood tryptic soy agar, was 4 × 10⁴ CFU/50 μg of lyophilized whole bacterial cells. Enzyme activities in these cells were blocked by a 15-min incubation with the Rgps inhibitor FPR-cmk and/or the Kgp inhibitor KYT-36 at room temperature (RT) before using the bacterial cells to stimulate human gingival epithelial Ca9-22 cells.

Cell and cell lines

The human gingival epithelial cell line Ca9-22 derived from squamous cell carcinoma was obtained from the Japanese Collection of Research Bioresources Cell Bank (Hokkaido, Japan). Ca9-22 cells were cultured in E-MEM (Wako Pure Chemical Industries) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (HyClone, Logan, UT) and 1% antibiotic-antimycotic mixture (Life Technologies, Grand Island, NY). Human oral epithelial cells (ScienCell Research Laboratories, Carlsbad, CA) were cultured in keratinocyte serum-free medium (Life Technologies). The ability of primary oral epithelial cells to adhere to culture plates slightly decreased after stimulation with P. gingivalis. Therefore, we analyzed the Ca9-22 cell line in this study, because it adhered more tightly to culture plates than primary oral epithelial cells after P. gingivalis stimulation. Cells were incubated in medium containing 5% FBS unless otherwise indicated.

IL-33 detection by immunohistochemistry

Gingival tissues from the junctional epithelium of patients with chronic periodontitis (n = 5) were obtained from teeth that required extraction at the time of initial examination. The criteria for chronic periodontitis comprised: probing pocket depth ≥ 5.0 mm, bleeding upon probing, clinical attachment loss ≥ 5.0 mm and radiographically confirmed extensive bone loss. The criteria for periodontally healthy individuals comprised: probing pocket depth < 3.0 mm, no bleeding upon probing, clinical attachment loss < 3.0 mm, and scarcely bone loss confirmed radiographically. Frozen gingival tissues embedded in OCT compound (Sakura Finetek, Tokyo, Japan) were sliced into 4-μm-thick sections. Endogenous peroxidase was blocked by incubation with 0.5% H₂O₂ for 5 min. The sections were then incubated with 2 μg/ml of rabbit anti-human IL-33 mAb (EPITOMICS, Burlingame, CA) or an isotype-matched control for 1 h at RT and with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Cell Signaling Technology, Beverly, MA). The sections were immunostained with 3,3’-diaminobenzidine (DAB) substrate (DakoCytomation, Carpinteria, CA), counterstained with Mayer’s hematoxylin and observed by microscopy.

Reverse transcription and real-time polymerase chain reaction (RT-qPCR)

Total cellular RNA was extracted using RNeasy^® (QIAGEN Inc., Valencia, CA) according to the manufacturer’s instructions and treated with DNase (RNase-free DNase set, QIAGEN). Total RNA (1 μg) was reverse-transcribed using Transcriptor First Strand cDNA Synthesis Kits^® (Roche Diagnostic Co., Indianapolis, IN) and a 1:10 volume of cDNA was theoretically converted from 100 ng of total RNA. Primers for PCR were designed using LightCycler probe design software^® (Roche Diagnostics GmbH, Mannheim, Germany), and comprised: IL-33, 5’-TTAAATAGGGTATTGGTAAAGAAACGG-3’ and 5’- AGTGTTTGAGCCTATCGTTTG-3’; IL-25, 5’-CCTACAGACAGGCTCCC-3’ and 5’-ACACACACAAGCTAAGGAAACA-3’; TSLP, 5’-TCTCAAATCTAGTTAGACAATTTGCAC-3’ and 5’-CATCCTGAAGCTGCCTATC-3’; PAR-1, 5’-TCGTCACTGCAGCATTT-3’ and 5’-AGATGGCCAGACAAGTGAA-3’; PAR-2, 5’-CTTTGCCGAAGTGTCCG-3’ and 5’-CCTACTGTGCAATTCCCA-3’; PAR-3, 5’-TTCCATTTGCCTTATTGCTACT-3’ and 5’-TGAGGGACCCTACAAAGATAGA-3’; PAR-4, 5’-GCTGTACTGGGTCGAAC-3’ and 5’-CCTGGCCTCTCCTTATCT-3’; GAPDH, 5’-TGAACCATGAGAAGTATGACAACA-3’ and 5’-TCTTCTGGGTGGCAGTG-3’. The amplification profile was 40 cycles at 94°C for 60 s, 55°C for 30 s, and 72°C for 30 s. PCR amplification proceeded using an Applied Biosystems 7300 Real-time PCR System^® (Life Technologies). The relative induction of IL-33 mRNA expression was determined after normalization using GAPDH as the reference gene and values are shown vs. control, which was converted to 1. For conventional PCR, The amplification profile was 30 cycles using the same program. Amplified samples were resolved on 2.0% agarose gels, stained with ethidium bromide and photographed under UV light.

Western blotting

Confluent cell monolayers were harvested using cell lysis buffer^® (Cell Signaling Technology) according to the manufacturer’s instructions. Nuclear and cytoplasmic extracts were separated using NE-PER nuclear and cytoplasmic extraction reagents (Pierce Biotechnology, Rockford, IL), respectively. Whole cell lysates, as well as nuclear and cytoplasmic fractions were separated by SDS-PAGE, and transferred to polyvinylidene difluoride membranes (ATTO, Tokyo, Japan) using a semidry transblot system (Bio-Rad Laboratories, Hercules, CA). Non-specific binding on the blots was blocked with 0.5% (w/v) non-fat dried milk and 0.1% (v/v) Tween 20 in PBS overnight at 4°C, followed by incubation for 1 h at RT with rabbit anti-human IL-33 mAb (EPITOMICS), mouse anti-human GAPDH mAb (MBL, Tokyo, Japan), rabbit anti-human p38 (Cell Signaling Technology), or with p38 polyclonal antibodies (Cell Signaling Technology) at 1:1,000 for 1 h at RT. Blots were incubated with HRP-conjugated goat anti-rabbit IgG (Cell Signaling Technology) at 1:2,000 for 1 h at RT and then soaked in Immunostar^® (Wako Pure Chemical Industries). Chemiluminescent signals were detected using an LAS-4000IR luminescent image analyzer (Fujifilm, Tokyo, Japan). The intensity of expression was quantified by densitometry using ImageJ software.

IL-33 detection by immunofluorescence assay

A suspension of Ca9-22 cells (3 × 10⁵/ml) was seeded overnight onto ibiTreat μ-Slide VI ^0.4 (ibidi GmbH, Martinsried, Germany) and stimulated with 50 μg/ml of whole P. gingivalis cells at MOI of 0.13 for 4 d to assess intracellular IL-33 expression. The cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with PBS containing 0.1% saponin for 10 min and then incubated in PBST containing 1% (w/v) BSA for 30 min to block non-specific binding. The cells were incubated for 1 h at 4°C with 0.5 μg/ml of phycoerythrin conjugated-rat anti-human IL-33 mAb (clone, 390412; R&D Systems, Minneapolis, MN). Thereafter, the cells were incubated with DAPI for 1 min to identify nuclei, mounted on slides using mounting medium, and assessed using fluorescence microscopy (BZ-9000, Keyence, Tokyo, Japan).

RNA interference

To inhibit human protease-activated receptor (PAR)-2 or β-arrestin-1 expression, Ca9-22 cells were transfected with Ambion Silencer^® Select Validated small interfering RNA (siRNA) for PAR-2, β-arrestin-1, or negative control (Life Technologies). Cells were seeded on a 24-well plates, and then transfected with 100 pmol of siRNA in 0.5 μl of Lipofectamine^® 2000 (Life Technologies) according to the manufacturer’s protocol. The transfected cells were stimulated 12 h later with whole P. gingivalis W83 cells for 48 h and assayed as described.

NF-κB luciferase assay

The pNFκB-Metridia luciferase reporter plasmid contains an NF-κB promoter element upstream of the secreted Metridia luciferase (MetLuc) gene (Clontech Laboratories, Mountain View, CA). The pMetridia luciferase reporter plasmid contains several cloning sites that allow the insertion of promoter and/or enhancer elements upstream of the secreted MetLuc gene (Clontech Laboratories), which serves as an internal standard of transfection. Transient transfection was achieved using Lipofectamine^® LTX (Life Technologies) according to the manufacturer’s instructions. Briefly, sub-confluent cells in 24-well plates were transiently transfected with 0.5 μg of the reporter construct and then stimulated 24 h later with whole P. gingivalis cells for 48 h in E-MEM containing 5% FBS. Secreted Metridia luciferase activity in culture supernatants was analyzed using Ready-To-Glow™ Secreted Luciferase Reporter Systems (Clontech Laboratories) and a SpectraMax M5 luminometer (Molecular Devices, Menlo Park, CA).

Measurement of IL-33 production by ELISA

Cells were stimulated with whole P. gingivalis cells for 48 h. Levels of IL-33 in culture supernatants were measured using IL-33 ELISA kits (eBioscience, San Diego, CA) according to the manufacturer’s instructions. The absorbance of IL-33 was determined using the SoftMax^® Pro data analysis program (Molecular Devices).

Enzyme activity assay

The amidolytic activities of gingipains secreted from whole P. gingivalis cells were assayed at 37°C using 0.5 mM N-α-benzoyl-_L-arginine-p-nitroanilide (BA-pNA) for Rgps and 0.5 mM Z-His-Glu-Lys-4-methylcoumaryl-7-amide (HEK-MCA) for Kgp in 1.0 ml of 0.2 M Tris-HCl, 0.1 M NaCl, 5 mM CaCl₂, and 10 mM cysteine (pH 7.6). The absorbance of p-nitroaniline or 7-amino-4-methylcoumarin released from the substrate was measured at 405 or 370 nm, respectively.

Statistical analysis

All experiments were repeated at least three times in triplicate to test the reproducibility of the results. Experimental data are representative results and are shown as means ± SD. The statistical significance of differences between control and experimental findings was evaluated by post-test after ANOVA. p-values < 0.05 were considered significant.

Results

Expression of IL-33 in human periodontal tissues

We initially analyzed IL-33 expression in gingival tissues from patients with chronic periodontitis by immunohistochemical staining to determine whether inflamed gingival epithelium expresses IL-33. Interleukin-33 was expressed in the cytoplasm of inflamed gingival epithelium from patients with chronic periodontitis (Fig 1D), but scarcely in that from healthy individuals (Fig 1B). In contrast, cytokines were weakly expressed in the lamina propria of gingival tissues (Fig 1B and 1D). The results from gingival tissues collected from at least four other patients with chronic periodontitis were similar (data not shown). An isotype-matched control antibody did not react with these tissues (Fig 1A and 1C). These findings suggest that gingival epithelium is a major source of IL-33 after infection with periodontal pathogens.

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Fig 1. Production of IL-33 is increased in chronic periodontitis.

Cryosections of normal periodontal tissues from healthy donors (A and B) and inflamed periodontal tissues from patients with chronic periodontitis (C and D) were stained with rabbit anti-human IL-33 mAb (B and D) or rabbit anti-human IgG isotype control (A and C) and HRP-conjugated anti-rabbit IgG and then visualized with DAB. Sections were counterstained with Mayer’s hematoxylin. GE, gingival epithelium; LP, lamina propria. Bar = 3 μm. Data are representative of five experiments.

https://doi.org/10.1371/journal.pone.0152794.g001

P. gingivalis increases IL-33 mRNA expression in human gingival epithelial cells

The suspected major pathogen associated with chronic periodontitis is P. gingivalis [23]. We therefore analyzed the effects of infection with viable P. gingivalis upon IL-33 mRNA expression in human gingival epithelial (Ca9-22) cells in vitro. The expression of IL-33 mRNA in Ca9-22 cells significantly increased from 24 to 48 h after infection with P. gingivalis W83 (Fig 2A and 2B) and after stimulation with lyophilized whole cells of P. gingivalis (Fig 2C). Therefore, IL-33 mRNA expression was mainly analyzed at 48 h after stimulation. We then examined whether IL-33 mRNA can be induced by bacterial cell wall components: fimbriae (TLR2 ligand) [31] and LPS (TLR2 and 4 ligand) [33] prepared from P. gingivalis, and synthetic P. gingivalis-type lipopeptide PGTP2-RL (TLR2/1 ligand) [35]. Fig 2D shows that IL-33 mRNA expression was significantly and dose-dependently enhanced after stimulation with > 1 μg/ml P. gingivalis W83, whereas fimbriae and PGTP-2-RL from P. gingivalis and the LPS preparations were inactive and slightly active, respectively. In addition to IL-33, IL-25, and TSLP are also produced by epithelial cells at mucosal surfaces, and they can induce the activation of Th2 cytokine-mediated immune responses [3]. Infection with P. gingivalis W83 (S1A and S1B Fig) or stimulation with whole P. gingivalis cells (S1C Fig) enhanced IL-25 and TSLP mRNA expression. In the presence of a protein synthesis inhibitor cycloheximide, P. gingivalis whole cells did not induce the expression of IL-33 mRNA in epithelial cells incubated with the protein synthesis inhibitor, cycloheximide (Fig 2E), indicating that the induction of IL-33 mRNA by P. gingivalis requires de novo protein synthesis. Furthermore, incubating the cells with nocodazole, a microtubule inhibitor, or with cytochalasin D, a particle internalization inhibitor, blocked IL-33 mRNA induction by P. gingivalis, suggesting that internalization of the bacterium is required for IL-33 mRNA induction (Fig 2F and 2G).

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Fig 2. P. gingivalis increases IL-33 mRNA expression in gingival epithelial cells.

Ca9-22 cells were infected with fresh P. gingivalis W83 cultures at MOI of 0.5 for the indicated periods (A) or indicated MOI for 48 h (B). Ca9-22 cells were stimulated with 50 μg/ml (MOI of 0.1) of whole P. gingivalis W83 cells for the indicated periods (C) and indicated amounts of whole P. gingivalis W83 cells, fimbriae, PGTP2-RL, and LPS derived from P. gingivalis for 48 h (D). Cells were incubated with 10 μg/ml cycloheximide for 45 min (E), 1 μg/ml nocodazole for 1 h (F) or 0.5 μM cytochalasin D for 30 min (G) and then stimulated for 48 h with 50 μg/ml of P. gingivalis W83 whole cells with the respective inhibitors. Total cellular RNA was then extracted at the indicated times, and IL-33 transcripts were analyzed by RT-qPCR. Data are representative of three independent experiments, and are shown as means ± SD of triplicate assays. Statistically significant differences are indicated. *, P<0.05 compared with untreated control; §, P<0.05 compared with P. gingivalis alone.

https://doi.org/10.1371/journal.pone.0152794.g002

P. gingivalis increases IL-33 protein expression

Stimulating human epithelial cells with extracellular ATP or applying mechanical stress without cell death results in the production of IL-33 [36, 37]. We examined whether P. gingivalis can induce IL-33 in Ca9-22 cells using Western blotting. Interleukin-33 was constitutively localized in the nuclei of resting Ca9-22 cells (S2 Fig) and its expression was increased 11.5-fold, reaching a peak at four days after P. gingivalis stimulation (Fig 3A). However, IL-33 levels in the supernatants from the same cultures were quite low (8–25 pg/ml) according to the ELISA findings (data not shown). Therefore, we examined the immunocytochemical localization of IL-33 protein in Ca9-22 cells after stimulation with P. gingivalis. Interleukin-33 accumulated in the cytoplasm of Ca9-22 cells after P. gingivalis stimulation for 4 d regardless of its constitutive localization in the nuclei of the resting cells (Fig 3B and S2 Fig).

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Fig 3. P. gingivalis increases IL-33 protein expression in gingival epithelial cells.

(A) Ca9-22 cells were stimulated with 50 μg/ml of whole P. gingivalis W83 cells for the indicated periods. Cell lysates were analyzed by Western blotting with an anti-human IL-33 mAb. Expression levels of IL-33 were quantified by densitometry using ImageJ software and normalized to medium alone. (B) Ca9-22 cells were stimulated with 50 μg/ml of whole P. gingivalis W83 cells for 4 d, and intracellular IL-33 protein was stained with PE-conjugated anti-human IL-33 mAb. Nuclei were stained with DAPI. Bar = 25 μm. Data are representative of three independent experiments.

https://doi.org/10.1371/journal.pone.0152794.g003

Gingipains participate in increased expression of IL-33 mRNA induced by P. gingivalis

We investigated whether or not gingipain enzyme activities are implicated in the ability of P. gingivalis to induce IL-33 in Ca9-22 cells and primary oral epithelial cells. The induction of IL-33 mRNA expression by P. gingivalis W83 whole cells was completely inhibited by Phe-Pro-Arg-chloromethyl ketone (FPR-cmk), an Rgp inhibitor and by KYT-36, a Kgp inhibitor in these cells, respectively (Fig 4A and 4B). The induction of IL-33 mRNA expression in Ca9-22 cells was attenuated by stimulation with P. gingivalis KDP131 (ΔrgpA), KDP129 (Δkgp), KDP133 (ΔrgpA ΔrgpB), and KDP136 (ΔrgpA ΔrgpB Δkgp) strains, but not with the KDP132 (ΔrgpB) strain compared with the P. gingivalis ATCC 33277 wild-type parent strain (Fig 4C). Therefore, whole P. gingivalis cells induced IL-33 mRNA expression dependently on RgpA and Kgp. Furthermore, stimulation with P. gingivalis KDP136 did not induce IL-33 mRNA expression in primary oral epithelial cells (Fig 4D). Incubating P. gingivalis for 1 h at 70°C to inactivate its enzyme activities completely ablated its ability to induce IL-33 mRNA (Fig 4E). We confirmed that the enzyme activities of Rgps and Kgp were intact in whole P. gingivalis ATCC 33277 wild-type parent cells, but not in those of comparable mutant P. gingivalis strains (S3 Fig). These findings indicate that the enzyme activity of gingipains is required for P. gingivalis to increase IL-33 expression in human gingival/oral epithelial cells.

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Fig 4. Participation of gingipains in P. gingivalis-induced IL-33 mRNA expression in gingival/oral epithelial cells.

Whole P. gingivalis W83 cells (50 μg/ml) were incubated with 0.3 μM FPR-cmk (Rgp inhibitor) or 0.3 μM KYT-36 (Kgp inhibitor) for 15 min at 37°C and then used to stimulate Ca9-22 (A) or primary oral epithelial (B) cells for 48 h. (C) Ca9-22 cells stimulated with 50 μg/ml of whole cells of P. gingivalis ATCC 33277 wild-type, P. gingivalis KDP131 (ΔrgpA), KDP132 (ΔrgpB), KDP129 (Δkgp), KDP133 (ΔrgpA ΔrgpB), or KDP136 (Δkgp ΔrgpA ΔrgpB) gingipain-null mutant for 48 h. (D) Primary oral epithelial cells stimulated with 50 μg/ml of P. gingivalis ATCC 33277 wild-type or KDP136 for 48 h. (E) Ca9-22 cells cultured for 48 h with 50 μg/ml of whole P. gingivalis W83 cells after incubation with or without at 70°C for 1 h. Total cellular RNA was extracted and transcripts were analyzed by RT-qPCR. Data are representative of three independent experiments, and are shown as means ± SD of triplicate assays. Statistical significant differences are indicated (*, P<0.05 compared with respective unstimulated control; §, P<0.05 compared with P. gingivalis alone).

https://doi.org/10.1371/journal.pone.0152794.g004

Involvement of PAR-2 and PLC in IL-33 induction by gingipains

Protease-activated receptor belongs to the G protein-coupled family of seven transmembrane domain receptors [38]. Both Rgps and Kgp activate PAR-1 and -2 expressed on human gingival epithelial cells, which produce proinflammatory cytokines [39, 40]. Resting Ca9-22 cells constitutively expressed PAR-1, -2, -3, and -4 mRNA (Fig 5A) and stimulation with whole P. gingivalis W83 cells increased PAR-2 mRNA expression (Fig 5B). To determine whether or not PAR-2 participates in IL-33 induction, we interfered with PAR-2 mRNA expression in Ca9-22 cells using a PAR-2-specific siRNA. The expression of PAR-2 mRNA was suppressed in 75% of cells transfected with PAR-2 siRNA compared with control siRNA (Fig 5C). The increased expression of IL-33 mRNA induced by whole P. gingivalis W83 cells was significantly inhibited in cells transfected with PAR-2 siRNA (Fig 5D). Because PAR-2 coupled to G protein is thought to result in the activation of phospholipase C (PLC) and protein kinase C (PKC) [41–43], we investigated whether the PLC pathway is involved in the induction of IL-33 mRNA expression by whole P. gingivalis in Ca9-22 cells. Incubating Ca9-22 cells with the PLC inhibitor U-73122 (1 μM), significantly inhibited subsequent IL-33 mRNA expression induced by P. gingivalis (Fig 5E). On the other hand, the PKC inhibitor, GF-109203X, did not inhibit either basal levels of IL-33 or the increase in IL-33 induced by P. gingivalis (Fig 5F). These findings suggest that the increase in IL-33 expression induced by P. gingivalis is mediated via the PAR-2-PLC signaling pathway.

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Fig 5. P. gingivalis-induced IL-33 mRNA expression requires activation of PAR-2 and PLC.

(A) Expression of PAR-1, -2, -3, and -4 mRNA by Ca9-22 cells analyzed by RT-PCR. Data are representative of two independent experiments. Fragments are 156 bp (GAPDH), 150 bp (PAR-1), 153 bp (PAR-2), 170 bp (PAR-3), and 177 bp (PAR-4), respectively. Ca9-22 cells were transfected with 100 pmol of PAR-2 siRNA or control siRNA for 14 h (C and D), and stimulated with 50 μg/ml of whole P. gingivalis W83 cells for 48 h (B and D). Ca9-22 cells were incubated with indicated concentrations of U-73122 (PLC inhibitor) (E) or GF109203X (PKC inhibitor) (F) for 30 min, and then stimulated with 50 μg/ml of whole P. gingivalis W83 cells for 48 h. Expression of PAR-2 mRNA (B and C) and IL-33 mRNA (D, E, and F) was analyzed using RT-qPCR. Data are representative of three independent experiments, and are shown as means ± SD of triplicate assays. Statistical significant differences are indicated (*, P<0.05 compared with respective unstimulated control; §, P<0.05 compared with P. gingivalis alone).

https://doi.org/10.1371/journal.pone.0152794.g005

Involvement of p38 and NF-κB in IL-33 induction by gingipains

Mitogen-activated protein (MAP) kinase (ERK, JNK, and p38) and NF-κB signaling pathways have been implicated in PAR-2-mediated activation [44, 45]. We therefore assessed whether or not p38 signaling is involved in P. gingivalis-induced, gingipain-mediated IL-33 regulation in Ca9-22 cells. The phosphorylation of p38 in cell lysates was enhanced after 9 h, and peaked at 18 h of stimulation with whole P. gingivalis W83 cells (Fig 6A). Incubating cells with the p38 inhibitor SB203580, significantly inhibited the induction of IL-33 mRNA by P. gingivalis (Fig 6B), whereas PD98059, an ERK1/2 inhibitor, and SP600125, a JNK inhibitor, did not (Fig 6B). Incubating bacterial cells with FPR-cmk plus KYT-36 completely inhibited the subsequent upregulation of p38 phosphorylation by P. gingivalis W83 (Fig 6C). Stimulation with P. gingivalis KDP136 did not generate detectable amounts of p38 phosphorylation (Fig 6D).

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Fig 6. Induction of IL-33 mRNA expression by P. gingivalis requires activation of the p38 pathway.

(A) Ca9-22 cells stimulated for indicated periods with 50 μg/ml of whole P. gingivalis W83 cells in medium containing 1% FBS. Phosphorylation of p38 was detected in cell lysates by Western blotting against anti-phospho-p38 antibody (p-p38). Controls comprised antibody against total p38. Data are representative of three independent experiments. Relative expression of phosphorylated p38 was quantified using densitometry. Relative expression of phosphorylated p38 was normalized to that of p38. (B) Ca9-22 cells incubated with 10 μM PD98059, SP600125, or SB203580 for 30 min and then stimulated with 50 μg/ml of whole P. gingivalis W83 cells for 48 h in medium containing 5% FBS. Total cellular RNA was extracted and transcripts were analyzed by RT-qPCR. Data are representative of three independent experiments, and are shown as means ± SD of triplicate assays. Statistical significant differences are indicated (*, P<0.05 compared with untreated control; §, P<0.05 compared with P. gingivalis alone). (C) Whole P. gingivalis W83 cells (50 μg/ml) were incubated with 0.3 μM FPR-cmk plus 0.3 μM KYT-36 for 15 min at 37°C and then used to stimulate Ca9-22 cells for 18 h in medium containing 1% FBS. (D) Ca9-22 cells were stimulated with 50 μg/ml of P. gingivalis ATCC 33277 wild-type or KDP 136 whole cells for 18 h. Cell lysates were analyzed by Western blotting using anti-phospho-p38 and anti-p38 antibodies. Data are representative of three independent experiments. Relative expression levels of phosphorylated p38 were normalized to levels of p38 and quantified by densitometry. Controls were adjusted to contain 0.1% (v/v) DMSO in media during incubation with inhibitors.

https://doi.org/10.1371/journal.pone.0152794.g006

We also used luciferase reporter assays to analyze whether or not NF-κB signaling is involved in the induction of IL-33 mRNA expression by P. gingivalis in Ca9-22 cells that were transiently transfected with a luciferase reporter construct containing the NF-κB response element promoter. Stimulation with P. gingivalis enhanced NF-κB activity after 3 h and this reached a peak after 24 h. Incubating P. gingivalis with FPR-cmk plus KYT-36 significantly reduced the subsequent induction of NF-κB activities by P. gingivalis (Fig 7B) in Ca9-22 cells. Stimulation with P. gingivalis KDP136 attenuated NF-κB activation in Ca9-22 cells (Fig 7C). Incubating cells with PDTC, an NF-κB inhibitor, significantly inhibited the subsequent induction of IL-33 mRNA expression by P. gingivalis (Fig 7D), but did not inhibit the phosphorylation of p38 induced by P. gingivalis at 18 h (Fig 7E). These results suggest that these two signaling pathways work independently of each other in P. gingivalis-induced IL-33 expression. Collectively, these results indicate that the increase in IL-33 expression induced by P. gingivalis and mediated by gingipain depends on PAR-2-PLC-p38/NF-κB signaling.

Download:

Fig 7. Induction of IL-33 mRNA expression by P. gingivalis requires activation of the NF-κB pathway.

(A) Ca9-22 cells stimulated with 50 μg/ml of whole P. gingivalis W83 cells for indicated periods. (B) Whole P. gingivalis W83 cells (50 μg/ml) were incubated with gingipain inhibitors (0.3 μM FPR-cmk plus 0.3 μM KYT-36) for 15 min at 37°C, and then used to stimulate Ca9-22 cells for 9 h. (C) Ca9-22 cells stimulated with 50 μg/ml of whole P. gingivalis ATCC 33277 wild-type or KDP136 cells for 9 h. (A-C) Cells were transiently transfected with pNFκB-Metridia luciferase reporter or control pMetridia luciferase reporter plasmids. Amount of secreted luciferase in culture supernatants were analyzed using a luminometer. (D) Ca9-22 cells incubated with indicated concentrations of PDTC (NF-κB inhibitor) for 1 h and then stimulated with 50 μg/ml of whole P. gingivalis W83 cells for 48 h. Total cellular RNA was extracted and transcripts were analyzed by RT-qPCR. Data are representative of three independent experiments and are shown as means ± SD of triplicate assays. Statistical significant differences are indicated (*, P<0.05 compared with respective unstimulated control; §, P<0.05 compared with P. gingivalis alone). (E) Ca9-22 cells were incubated with 25 μM PCTC for 1 h and then stimulated for 18 h with 50 μg/ml of whole P. gingivalis W83 cells in medium containing 1% FBS. Cell lysates were analyzed by Western blotting against anti-phospho-p38 and anti-p38 antibodies. Data are representative of three independent experiments. Relative expression levels of phosphorylated p38 were normalized to p38 and quantified by densitometry. Control media was adjusted to contain 0.1% (v/v) DMSO during incubation with inhibitor.

https://doi.org/10.1371/journal.pone.0152794.g007

Discussion

Increasing evidence suggests that epithelium-derived cytokines, including IL-33, IL-25, and TSLP, are critical regulators of Th2 cytokine-mediated inflammation at mucosal sites [3]. The present study found that IL-33 expression was increased in gingival epithelia from patients with chronic periodontitis. We also established that the elevated expression of IL-33 induced by P. gingivalis was mediated in part via PAR-2 through gingipain-dependent activation in human gingival epithelial cells. Interleukin-33 expression induced by P. gingivalis was mediated by the PLC, p38, and NF-κB signaling pathways. These results suggest that P. gingivalis-induced IL-33 expression is associated with the pathogenesis of periodontitis.

Protease-activated receptor-2 is constitutively expressed on gingival epithelial cells from healthy individuals [39] and increased in parallel with the prevalence of P. gingivalis in gingival crevicular fluid (GCF) from patients with chronic periodontitis [46, 47]. Periodontal treatment decreases PAR-2 expression [46, 48]. Consistent with these facts, PAR-2 expression is increased in response to P. gingivalis whole cells (Fig 5B) and culture supernatants [49] in human gingival epithelial cells. P. gingivalis-induced IL-33 expression was attenuated in PAR-2 knockdown Ca9-22 cells (Fig 5D). Cellular activation is mediated by PAR-2 binding to ligand after the receptor is activated by proteolysis, or to tethered ligand peptide agonists in a proteolysis-independent manner [38]. We determined that IL-33 expression needed to be increased by P. gingivalis for the enzymatic activation of gingipains, because heating the bacteria to inactivate the enzymatic activities of gingipains abolished the subsequent P. gingivalis-mediated increase in IL-33 mRNA (Fig 4E). The expression of TSLP or IL-25 is induced in bronchial epithelial cells via PAR-2 by serine proteases from airborne pathogens, such as Alternaria or house dust mites [50, 51]. We confirmed that TSLP and IL-25 mRNA expression was obviously and gingipain-dependently increased by fresh P. gingivalis cultures (S1A and S1B Fig) and by whole P. gingivalis cells (S1C and S4 Figs), similar to that of IL-33 mRNA expression in Ca9-22 cells. The production of Th2 cytokines and chemokines from human mast cells in response to IL-33 is enhanced by adding TSLP [52]. Cross-regulation among IL-33, IL-25, and TSLP might play important roles in the persistence of Th2 cytokine-mediated inflammation. The activation of PAR-2 induces canonical G protein and β-arrestin-dependent signaling followed by the translocation of β-arrestin-1 from the cytosol to the plasma membrane and the subsequent endocytosis of PAR-2-β-arrestin-1 complexes in early endosomes [38, 53]. However, the internalization of phosphorylated-PAR-2 is through a canonical dynamin-, clathrin-, and β-arrestin-dependent pathway in this context [54]. The expression of IL-33 mRNA induced by P. gingivalis was partly attenuated in β-arrestin-1 knockdown Ca9-22 cells compared with control cells (S5 Fig). These results suggest that activated-PAR-2 internalization and the formation of PAR-2-β-arrestin-1 complexes are required for P. gingivalis-induced IL-33 induction. However, the processes of P. gingivalis-induced β-arrestin-1 mobilization and association with P. gingivalis in Ca9-22 cells remain unclear. Lamont et al. (1995) reported that P. gingivalis invasion of oral epithelial cells is inhibited in the presence of cytochalasin D which also inhibits actin polymerization, or nocodazole which depolymerizes microtubules [55]. The present study found that blocking P. gingivalis invasion using cytochalasin D or nocodazole impaired P. gingivalis-induced IL-33 expression (Fig 2F and 2G). The above and the present findings suggest that P. gingivalis invasion is necessary to increase IL-33 expression. Others have shown that P. gingivalis invades gingival epithelial cells via endocytosis, which is mediated by the binding of RgpA adhesin peptide A44 to host cells [56]. Both RgpA and Kgp have hemagglutinin/adhesin domains that interact with fibrinogen, fibronectin and laminin, whereas RgpB does not [57]. The up-regulation of IL-33 by P. gingivalis was not attenuated by stimulation with P. gingivalis KDP132 (ΔrgpB), which lacks hemagglutinin/adhesin domains (Fig 4C). Gingipains are notably essential for the maturation of P. gingivalis fimbriae [58], which are required for P. gingivalis attachment [59] and internalization into epithelial cells [60]. However, it is unknown whether purified gingipains induce IL-33 production in human gingival epithelial cells. Further experiments are needed to understand this. These findings, in conjunction with our results, suggest that both P. gingivalis-induced PAR-2 activation and the endocytosis of P. gingivalis conceivably participate in the increased expression of IL-33 in gingival epithelial cells.

We detected p38 phosphorylation in Ca9-22 cells at 18 h after stimulation with whole P. gingivalis cells (Fig 6A). The upregulation of IL-33 mRNA by whole P. gingivalis cells was not mediated via either the ERK or the JNK pathways (Fig 6B). One study found that ERK and JNK became phosphorylated in human gingival epithelial cells at 5 min after infection with P. gingivalis whereas p38 did not [61]. In contrast, CCL20 mRNA that is expressed in human gingival epithelial cells at 16 h after infection with P. gingivalis is blocked by inhibiting p38, but not JNK [62]. This suggests that p38 activation is involved in the late-phase response to IL-33 regulation. We clarified that the increased expression of IL-33 was induced by P. gingivalis through gingipains via the PAR-2, PLC, p38, and NF-κB signaling pathways.

The expression of IL-33 is increased in response to proteases, pathogen-associated molecular patterns (PAMPs), and proinflammatory stimuli [1, 6, 63, 64]. P. gingivalis possesses various virulence factors, such as LPS (TLR2 and TLR4 ligand) [33], fimbriae (TLR2 ligand) [31], and lipoprotein (TLR2/1 ligand) [35]. The present study found that fimbriae and PGTP2-RL, a lipopeptide from P. gingivalis, were completely inactive, whereas P. gingivalis LPS was only weakly active in human gingival epithelial cells (Fig 2D), due to low levels of TLR2 expression in these cells [65]. Nile et al. reported that P. gingivalis LPS (TLR2 and TLR4 ligand) induces IL-33 expression in human monocytes [6]. We also determined that IL-33 expression is increased by fimbriae or PGTP2-RL in bone marrow-derived dendritic cells from WT mice, but not from TLR2-deficient mice (unpublished data).

Full-length IL-33 is released from necrotic cells and subsequently acts as an alarmin [6, 66, 67, 68]. Stimulation with extracellular ATP or mechanical stress without cell death also results in IL-33 production [36, 37]. Levels of IL-33 in GCF do not differ between healthy donors and patients with chronic periodontitis [69] and IL-33 is not a component of GCF in inflamed periodontal tissues from patients with chronic periodontitis [70]. Indeed, our ELISA findings indicated very low levels of IL-33 (25 pg/ml) in supernatants from Ca9-22 cells stimulated with 50 μg/ml of P. gingivalis W83 for 4 d (data not shown). Interleukin-33 localized in the nucleus of resting Ca9-22 cells (Fig 3B and S2 Fig), whereas IL-33 induced by P. gingivalis re-localized in the cytoplasm of Ca9-22 cells (Fig 3B). The endogenous full-length IL-33 (30 kDa) induced by P. gingivalis in the total cell lysate appeared to have biological activity (Fig 3A). When Ca9-22 cells were incubated for up to 4 d with 50 μg/ml of whole P. gingivalis W83 cells, lactate dehydrogenase (an indicator of necrosis) was detected in the culture supernatants at 2 d (S6B Fig). These results suggest that a small amount of P. gingivalis-induced IL-33 is released from the cells after necrosis. Cell viability was slightly reduced in the cells stimulated with P. gingivalis at 1 to 2 d after incubation, but not at 4 d after incubation (S6A Fig). Gingipains can directly cleave cytokines such as TNF-α [71] and IL-8 [26], and induce caspase-3-dependent apoptosis in human gingival epithelial cells [72]. Caspase-3 proteolytically processes IL-33 to the inactive form after apoptotic cell death [66, 67]. Under these experimental conditions, IL-33 might be directly cleaved by gingipains or inactivated by caspase-3. However, whether or not the action of released IL-33 as an alarmin in periodontal tissues is gingipain concentration-dependent remains unknown. The turnover rate of gingival epithelial cells is 4–6 days [73], which is high enough to allow rapid replacement of the cells by microbial challenge. Alternatively, IL-33 acts as a dual-functional molecule that works as an endogenous nuclear protein [74]. Ali et al. reported that full-length IL-33, which localized in both the cytosol and nucleus, interacts with NF-κB p65 and impairs p65-mediated transactivation [75]. Thus the accumulation of intracellular IL-33 induced by P. gingivalis might attenuate the p65-mediated proinflammatory reaction in gingival epithelial cells. The function of IL-33 induced by P. gingivalis in epithelial cells might primarily be intracellular, and secondarily as an alarmin upon release into the extracellular milieu. The roles of IL-33 derived from gingival epithelial cells, which could include the modulation of innate immune function, need to be defined by further studies and the implications for the pathogenesis of periodontal diseases need to be assessed. Infection with P. gingivalis appears to promote the PAR-2/IL-33 axis through a gingipain-dependent mechanism in human gingival epithelial cells.

Supporting Information

S1 Fig. P. gingivalis increases IL-25 and TSLP mRNA expressions in gingival epithelial cells.

Ca9-22 cells were infected with fresh P. gingivalis W83 cultures at an MOI of 0.5 for the indicated periods (A), or at the indicated MOI for 48 h (B). (C) Ca9-22 cells were stimulated for 48 h with indicated concentrations of whole P. gingivalis W83 cells. Total cellular RNA was extracted and transcripts of IL-25 and TSLP were analyzed by RT-qPCR. Data are representative of three independent experiments, and are shown as means ± SD of triplicate assays. Statistical significant differences are indicated (*, P<0.05 compared with untreated control).

https://doi.org/10.1371/journal.pone.0152794.s001

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S2 Fig. Nuclear expression of IL-33 in resting gingival epithelial cells.

Cell lysates, cytoplasmic extracts or nuclear extracts from Ca9-22 cells were analyzed by Western blotting with anti-human IL-33 mAb or anti-human GAPDH mAb. Data are representative of three independent experiments.

https://doi.org/10.1371/journal.pone.0152794.s002

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S3 Fig. Amidolytic activities of various gingipain-mutant strains of whole P. gingivalis cells.

Amidolytic activities of P. gingivalis whole cells were assayed at 37°C with 0.5 mM BA-pNA (A) or 0.5 mM HEK-MCA (B) for 1 h. Results are expressed as absorbance of p-nitroaniline (A) or 7-amino-4-methylcoumarin (B) released from substrate, respectively.

https://doi.org/10.1371/journal.pone.0152794.s003

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S4 Fig. Involvement of gingipains in P. gingivalis-induced IL-25 and TSLP mRNA expression in gingival epithelial cells.

(A and B) Whole P. gingivalis W83 cells (50 μg/ml) were incubated with 0.3 μM FPR-cmK or 0.3 μM KYT-36 for 15 min at 37°C. Ca9-22 cells (A) or primary oral epithelial cells (B) were stimulated with the treated or untreated P. gingivalis whole cells for 48 h. Ca9-22 (C) or primary oral epithelial (D) cells were stimulated with 50 μg/ml of P. gingivalis ATCC 33277 wild-type or KDP136 for 48 h. Total cellular RNA was extracted and IL-25 and TSLP transcripts were analyzed by RT-qPCR. Data are representative of three independent experiments and are shown as means ± SD of triplicate assays. Statistical significant differences are indicated (*, P<0.05 compared with respective unstimulated control; §, P<0.05 compared with P. gingivalis alone).

https://doi.org/10.1371/journal.pone.0152794.s004

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S5 Fig. P. gingivalis-induced IL-33 mRNA expression partially requires expression of β-arrestin-1.

Ca9-22 cells were transfected with 100 pmol of β-arrestin-1 siRNA or control siRNA for 12 h, and cells were stimulated with 50 μg/ml of whole P. gingivalis W83 cells for 48 h. The expression of IL-33 mRNA was analyzed by RT-qPCR. Data are representative of three independent experiments, and are shown as means ± SD of triplicate assays. Statistical significant differences are indicated (*, P<0.05 compared with respective unstimulated control; §, P<0.05 compared with negative control siRNA).

https://doi.org/10.1371/journal.pone.0152794.s005

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S6 Fig. Cell viability and cytotoxicity of gingival epithelial cells after stimulation with P. gingivalis.

Ca9-22 cells were stimulated with 50 μg/ml of whole P. gingivalis W83 cells for the indicated periods, and then the luminescence of NanoLuc substrate (A) or the absorbance of LDH (B) was measured using a luminometer or spectrophotometer, respectively. Data are representative of three independent experiments and are shown as means ± SD of triplicate assays.

https://doi.org/10.1371/journal.pone.0152794.s006

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Acknowledgments

We express our sincere thanks to M. Naito and K. Nakayama (Nagasaki University) for providing various mutant P. gingivalis strains, which enabled us to investigate the role of gingipains. We also thank R. Isoda and K. Kobayashi (National Center for Geriatrics and Gerontology), K. Nagano and F. Yoshimura (Aichi Gakuin University), S. Kawabata (Osaka University) and R. Tamai (Ohu University) for technical support. We are grateful to H. Takada (Tohoku University) for support and critical reading of the manuscript and to D. Mrozek (Medical English Service, Kyoto, Japan) for review of the manuscript.

Author Contributions

Conceived and designed the experiments: HT KM. Performed the experiments: HT TM TN. Analyzed the data: HT TM TN KM. Contributed reagents/materials/analysis tools: HT TM TN MH YK HS KM. Wrote the paper: HT KM.

References

1. Schmitz J, Owyang A, Oldham E, Song Y, Murphy E, McClanahan TK, et al. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity. 2015; 23: 479–490.
- View Article
- Google Scholar
2. Moussion C, Ortega N, Girard JP. The IL-1-like cytokine IL-33 is constitutively expressed in the nucleus of endothelial cells and epithelial cells in vivo: a novel 'alarmin'? PLoS One. 2008; 3: e3331. pmid:18836528
- View Article
- PubMed/NCBI
- Google Scholar
3. Saenz SA, Taylor BC, Artis D. Welcome to the neighborhood: epithelial cell-derived cytokines license innate and adaptive immune responses at mucosal sites. Immunol Rev. 2008; 226: 172–190. pmid:19161424
- View Article
- PubMed/NCBI
- Google Scholar
4. Paul WE, Zhu J. How are T_H2-type immune responses initiated and amplified? Nat Rev Immunol. 2010; 10: 225–235. pmid:20336151
- View Article
- PubMed/NCBI
- Google Scholar
5. Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT. Induction of IL-33 expression and activity in central nervous system glia. J Leukoc Biol. 2008; 84: 631–643. pmid:18552204
- View Article
- PubMed/NCBI
- Google Scholar
6. Nile CJ, Barksby E, Jitprasertwong P, Preshaw PM, Taylor JJ. Expression and regulation of interleukin-33 in human monocytes. Immunology. 2010; 130: 172–180. pmid:20070408
- View Article
- PubMed/NCBI
- Google Scholar
7. Shimosato T, Fujimoto M, Tohno M, Sato T, Tateo M, Otani H, et al. CpG oligodeoxynucleotides induce strong up-regulation of interleukin 33 via Toll-like receptor 9. Biochem Biophys Res Commun. 2010; 394: 81–86. pmid:20178773
- View Article
- PubMed/NCBI
- Google Scholar
8. Meephansan J, Tsuda H, Komine M, Tominaga S, Ohtsuki M. Regulation of IL-33 expression by IFN-γ and tumor necrosis factor-α in normal human epidermal keratinocytes. J Invest Dermatol. 2012; 132: 2593–2600. pmid:22673732
- View Article
- PubMed/NCBI
- Google Scholar
9. Le H, Kim W, Kim J, Cho HR, Kwon B. Interleukin-33: a mediator of inflammation targeting hematopoietic stem and progenitor cells and their progenies. Front Immunol. 2013; 4: 1–9. pmid:23653627
- View Article
- PubMed/NCBI
- Google Scholar
10. Jones LA, Roberts F, Nickdel MB, Brombacher F, McKenzie ANJ, Henriquez FL, et al. IL-33 receptor (T1/ST2) signalling is necessary to prevent the development of encephalitis in mice infected with Toxoplasma gondii. Eur J Immunol. 2010; 40: 426–436. pmid:19950183
- View Article
- PubMed/NCBI
- Google Scholar
11. Moro K, Yamada T, Tanabe M, Takeuchi T, Ikawa T, Kawamoto H, et al. Innate production of T_H2 cytokines by adipose tissue-associated c-Kit⁺Sca-1⁺ lymphoid cells. Nature. 2010; 463: 540–544. pmid:20023630
- View Article
- PubMed/NCBI
- Google Scholar
12. Neill DR, Wong SH, Bellosi A, Flynn RJ, Daly M, Langford TKA, et al. Nuocytes represent a new innate effector leukocyte that mediates type-2 immunity. Nature. 2010; 464: 1367–1370. pmid:20200518
- View Article
- PubMed/NCBI
- Google Scholar
13. Price AE, Liang HE, Sullivan BM, Reinhardt RL, Eisley CJ, Erle DJ, et al. Systemically dispersed innate IL-13-expressing cells in type 2 immunity. Proc Natl Acad Sci U S A. 2010; 107: 11489–11494. pmid:20534524
- View Article
- PubMed/NCBI
- Google Scholar
14. Yasuda K, Muto T, Kawagoe T, Matsumoto M, Sasaki Y, Matsushita K, et al. Contribution of IL-33-activated type II innate lymphoid cells to pulmonary eosinophilia in intestinal nematode-infected mice. Proc Natl Acad Sci U S A. 2012; 109: 3451–3456. pmid:22331917
- View Article
- PubMed/NCBI
- Google Scholar
15. Préfontaine D, Lajoie-Kadoch S, Foley S, Audusseau S, Olivenstein R, Halayko AJ, et al. Increased expression of IL-33 in severe asthma: evidence of expression by airway smooth muscle cells. J Immunol. 2009; 183: 5094–5103. pmid:19801525
- View Article
- PubMed/NCBI
- Google Scholar
16. Haenuki Y, Matsushita K, Futatsugi-Yumikura S, Ishii KJ, Kawagoe T, Imoto Y, et al. A critical role of IL-33 in experimental allergic rhinitis. J Allergy Clin Immunol. 2012; 130: 184–194. pmid:22460070
- View Article
- PubMed/NCBI
- Google Scholar
17. Byers DE, Alexander-Brett J, Patel AC, Agapov E, Dang-Vu G, Jin X, et al. Long-term IL-33-producing epithelial progenitor cells in chronic obstructive lung disease. J Clin Invest. 2013; 123: 3967–3982. pmid:23945235
- View Article
- PubMed/NCBI
- Google Scholar
18. Schiering C, Krausgruber T, Chomka A, Fröhlich A, Adelmann K, Wohlfert EA, et al. The alarmin IL-33 promotes regulatory T-cell function in the intestine. Nature. 2014; 513: 564–568. pmid:25043027
- View Article
- PubMed/NCBI
- Google Scholar
19. Chang YJ, Kim HY, Albacker LA, Baumgarth N, McKenzie ANJ, Smith DE, et al. Innate lymphoid cells mediate influenza-induced airway hyper-reactivity independently of adaptive immunity. Nat Immunol. 2011; 12: 631–638. pmid:21623379
- View Article
- PubMed/NCBI
- Google Scholar
20. Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H. IL-33-responsive lineage^- CD25⁺ CD44^hi lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs. J Immunol. 2012; 188: 1503–1513. pmid:22198948
- View Article
- PubMed/NCBI
- Google Scholar
21. Kim HY, Chang YJ, Subramanian S, Lee HH, Albacker LA, Matangkasombut P, et al. Innate lymphoid cells responding to IL-33 mediate airway hyperreactivity independently of adaptive immunity. J Allergy Clin Immunol. 2012; 129: 216–227. pmid:22119406
- View Article
- PubMed/NCBI
- Google Scholar
22. Enoksson M, Lyberg K, Möller-Westerberg C, Fallon PG, Nilsson G, Lunderius-Andersson C. Mast cells as sensors of cell injury through IL-33 recognition. J Immunol. 2011; 186: 2523–2528. pmid:21239713
- View Article
- PubMed/NCBI
- Google Scholar
23. Pathirana RD, O'Brien-Simpson NM, Reynolds EC. Host immune responses to Porphyromonas gingivalis antigens. Periodontol 2000. 2010; 52: 218–237. pmid:20017803
- View Article
- PubMed/NCBI
- Google Scholar
24. Potempa J, Sroka A, Imamura T, Travis J. Gingipains, the major cysteine proteinases and virulence factors of Porphyromonas gingivalis: structure, function and assembly of multidomain protein complexes. Curr Protein Pept Sci. 2003; 4: 397–407. pmid:14683426
- View Article
- PubMed/NCBI
- Google Scholar
25. Jagels MA, Travis J, Potempa J, Pike R, Hugli TE. Proteolytic inactivation of the leukocyte C5a receptor by proteinases derived from Porphyromonas gingivalis. Infect Immun. 1996; 64: 1984–1991. pmid:8675297
- View Article
- PubMed/NCBI
- Google Scholar
26. Mikolajczyk-Pawlinska J, Travis J, Potempa J. Modulation of interleukin-8 activity by gingipains from Porphyromonas gingivalis: implications for pathogenicity of periodontal disease. FEBS Lett. 1998; 440: 282–286. pmid:9872387
- View Article
- PubMed/NCBI
- Google Scholar
27. Yilmaz Ö, Verbeke P, Lamont RJ, Ojcius DM. Intercellular spreading of Porphyromonas gingivalis infection in primary gingival epithelial cells. Infect Immun. 2006; 74: 703–710. pmid:16369027
- View Article
- PubMed/NCBI
- Google Scholar
28. Tada H, Shimauchi H, Takada H, Matsushita K. Possible roles of IL-33 in periodontal diseases: Porphyromonas gingivalis induced IL-33 in human gingival epithelial cells. In: Sasaki K, Suzuki O, Takahashi N, editors. Interface Oral Health Science. Tokyo: Springer Japan; 2015. pp. 293–303.
29. Malcolm J, Awang RA, Oliver-Bell J, Butcher JP, Campbell L, Adrados Planell A, et al. IL-33 exacerbates periodontal disease through induction of RANKL. J Dent Res. 2015; 94: 968–975. pmid:25808546
- View Article
- PubMed/NCBI
- Google Scholar
30. Ogawa T, Shimauchi H, Hamada S. Mucosal and systemic immune responses in BALB/c mice to Bacteroides gingivalis fimbriae administered orally. Infect Immun. 1989; 57: 3466–3471. pmid:2572554
- View Article
- PubMed/NCBI
- Google Scholar
31. Ogawa T, Asai Y, Hashimoto M, Uchida H. Bacterial fimbriae activate human peripheral blood monocytes utilizing TLR2, CD14 and CD11a/CD18 as cellular receptors. Eur J Immunol. 2002; 32: 2543–2550. pmid:12207338
- View Article
- PubMed/NCBI
- Google Scholar
32. Makimura Y, Asai Y, Taiji Y, Sugiyama A, Tamai R, Ogawa T. Correlation between chemical structure and biological activities of Porphyromonas gingivalis synthetic lipopeptide derivatives. Clin Exp Immunol. 2006; 146: 159–168. pmid:16968410
- View Article
- PubMed/NCBI
- Google Scholar
33. Darveau RP, Pham TT, Lemley K, Reife RA, Bainbridge BW, Coats SR, et al. Porphyromonas gingivalis lipopolysaccharide contains multiple lipid A species that functionally interact with both Toll-like receptors 2 and 4. Infect Immun. 2004; 72: 5041–5051. pmid:15321997
- View Article
- PubMed/NCBI
- Google Scholar
34. Shi Y, Ratnayake DB, Okamoto K, Abe N, Yamamoto K, Nakayama K. Genetic analyses of proteolysis, hemoglobin binding, and hemagglutination of Porphyromonas gingivalis. J Biol Chem. 1999; 274: 17955–17960. pmid:10364243
- View Article
- PubMed/NCBI
- Google Scholar
35. Hashimoto M, Asai Y, Ogawa T. Separation and structural analysis of lipoprotein in a lipopolysaccharide preparation from Porphyromonas gingivalis. Int Immunol. 2004; 16: 1431–1437. pmid:15326096
- View Article
- PubMed/NCBI
- Google Scholar
36. Kouzaki H, Iijima K, Kobayashi T, O'Grady SM, Kita H. The danger signal, extracellular ATP, is a sensor for an airborne allergen and triggers IL-33 release and innate Th2-type responses. J Immunol. 2011; 186: 4375–4387. pmid:21357533
- View Article
- PubMed/NCBI
- Google Scholar
37. Kakkar R, Hei H, Dobner S, Lee RT. Interleukin 33 as a mechanically responsive cytokine secreted by living cells. J Biol Chem. 2012; 287: 6941–6948. pmid:22215666
- View Article
- PubMed/NCBI
- Google Scholar
38. Rothmeier AS, Ruf W. Protease-activated receptor 2 signaling in inflammation. Semin Immunopathol. 2012; 34: 133–149. pmid:21971685
- View Article
- PubMed/NCBI
- Google Scholar
39. Lourbakos A, Potempa J, Travis J, D'Andrea MR, Andrade-Gordon P, Santulli R, et al. Arginine-specific protease from Porphyromonas gingivalis activates protease-activated receptors on human oral epithelial cells and induces interleukin-6 secretion. Infect Immun. 2001; 69: 5121–5130. pmid:11447194
- View Article
- PubMed/NCBI
- Google Scholar
40. Giacaman RA, Asrani AC, Ross KF, Herzberg MC. Cleavage of protease-activated receptors on an immortalized oral epithelial cell line by Porphyromonas gingivalis gingipains. Microbiology. 2009; 155: 3238–3246. pmid:19608609
- View Article
- PubMed/NCBI
- Google Scholar
41. Böhm SK, Khitin LM, Grady EF, Aponte G, Payan DG, Bunnett NW. Mechanisms of desensitization and resensitization of proteinase-activated receptor-2. J Biol Chem. 1996; 271: 22003–22016. pmid:8703006
- View Article
- PubMed/NCBI
- Google Scholar
42. Böhm SK, Kong W, Brömme D, Smeekens SP, Anderson DC, Connolly A, et al. Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2. Biochem J. 1996; 314: 1009–1016. pmid:8615752
- View Article
- PubMed/NCBI
- Google Scholar
43. Seatter MJ, Drummond R, Kanke T, Macfarlane SR, Hollenberg MD, Plevin R. The role of the C-terminal tail in protease-activated receptor-2-mediated Ca²⁺ signalling, proline-rich tyrosine kinase-2 activation, and mitogen-activated protein kinase activity. Cell Signal. 2004; 16: 21–29. pmid:14607272
- View Article
- PubMed/NCBI
- Google Scholar
44. Belham CM, Tate RJ, Scott PH, Pemberton AD, Miller HR, Wadsworth RM, et al. Trypsin stimulates proteinase-activated receptor-2-dependent and -independent activation of mitogen-activated protein kinases. Biochem J. 1996; 320: 939–946. pmid:9003384
- View Article
- PubMed/NCBI
- Google Scholar
45. Macfarlane SR, Sloss CM, Cameron P, Kanke T, McKenzie RC, Plevin R. The role of intracellular Ca²⁺ in the regulation of proteinase-activated receptor-2 mediated nuclear factor kappa B signalling in keratinocytes. Br J Pharmacol. 2005; 145: 535–544. pmid:15821758
- View Article
- PubMed/NCBI
- Google Scholar
46. Holzhausen M, Cortelli JR, da Silva VA, Franco GC, Cortelli SC, Vergnolle N. Protease-activated receptor-2 (PAR₂) in human periodontitis. J Dent Res. 2010; 89: 948–953. pmid:20530726
- View Article
- PubMed/NCBI
- Google Scholar
47. Fagundes JA, Monoo LD, Euzébio Alves VT, Pannuti CM, Cortelli SC, Cortelli JR, et al. Porphyromonas gingivalis is associated with protease-activated receptor-2 upregulation in chronic periodontitis. J Periodontol. 2011; 82: 1596–1601. pmid:21513479
- View Article
- PubMed/NCBI
- Google Scholar
48. Euzebio Alves VT, Bueno da Silva HA, de França BN, Eichler RS, Saraiva L, Catalli de Carvalho MH, et al. Periodontal treatment downregulates protease-activated receptor 2 in human gingival crevicular fluid cells. Infect Immun. 2013; 81: 4399–4407. pmid:24042113
- View Article
- PubMed/NCBI
- Google Scholar
49. Dommisch H, Chung WO, Rohani MG, Williams D, Rangarajan M, Curtis MA, et al. Protease-activated receptor 2 mediates human beta-defensin 2 and CC chemokine ligand 20 mRNA expression in response to proteases secreted by Porphyromonas gingivalis. Infect Immun. 2007; 75: 4326–4333. pmid:17591792
- View Article
- PubMed/NCBI
- Google Scholar
50. Kouzaki H, O'Grady SM, Lawrence CB, Kita H. Proteases induce production of thymic stromal lymphopoietin by airway epithelial cells through protease-activated receptor-2. J Immunol. 2009; 183: 1427–1434. pmid:19561109
- View Article
- PubMed/NCBI
- Google Scholar
51. Kouzaki H, Tojima I, Kita H, Shimizu T. Transcription of interleukin-25 and extracellular release of the protein is regulated by allergen proteases in airway epithelial cells. Am J Respir Cell Mol Biol. 2013; 49: 741–750. pmid:23590308
- View Article
- PubMed/NCBI
- Google Scholar
52. Allakhverdi Z, Smith DE, Comeau MR, Delespesse G. The ST2 ligand IL-33 potently activates and drives maturation of human mast cells. J Immunol. 2007; 179: 2051–2054. pmid:17675461
- View Article
- PubMed/NCBI
- Google Scholar
53. DeFea KA, Zalevsky J, Thoma MS, Déry O, Mullins RD, Bunnett NW. β-arrestin-dependent endocytosis of proteinase-activated receptor 2 is required for intracellular targeting of activated ERK1/2. J Cell Biol. 2000; 148: 1267–1281. pmid:10725339
- View Article
- PubMed/NCBI
- Google Scholar
54. Ricks TK, Trejo J. Phosphorylation of protease-activated receptor-2 differentially regulates desensitization and internalization. J Biol Chem. 2009; 284: 34444–34457. pmid:19815543
- View Article
- PubMed/NCBI
- Google Scholar
55. Lamont RJ, Chan A, Belton CM, Izutsu KT, Vasel D, Weinberg A. Porphyromonas gingivalis invasion of gingival epithelial cells. Infect Immun. 1995; 63: 3878–3885. pmid:7558295
- View Article
- PubMed/NCBI
- Google Scholar
56. Boisvert H, Duncan MJ. Clathrin-dependent entry of a gingipain adhesin peptide and Porphyromonas gingivalis into host cells. Cell Microbiol. 2008; 10: 2538–2552. pmid:18717820
- View Article
- PubMed/NCBI
- Google Scholar
57. Pike RN, Potempa J, McGraw W, Coetzer THT, Travis J. Characterization of the binding activities of proteinase-adhesin complexes from Porphyromonas gingivalis. J Bacteriol. 1996; 178: 2876–2882. pmid:8631676
- View Article
- PubMed/NCBI
- Google Scholar
58. Nakayama K, Yoshimura F, Kadowaki T, Yamamoto K. Involvement of arginine-specific cysteine proteinase (Arg-gingipain) in fimbriation of Porphyromonas gingivalis. J Bacteriol. 1996; 178: 2818–2824. pmid:8631669
- View Article
- PubMed/NCBI
- Google Scholar
59. Kato T, Tsuda T, Omori H, Kato T, Yoshimori T, Amano A. Maturation of fimbria precursor protein by exogenous gingipains in Porphyromonas gingivalis gingipain-null mutant. FEMS Microbiol Lett. 2007; 273: 96–102. pmid:17559394
- View Article
- PubMed/NCBI
- Google Scholar
60. Amano A, Nakagawa I, Okahashi N, Hamada N. Variations of Porphyromonas gingivalis fimbriae in relation to microbial pathogenesis. J Periodontal Res. 2004; 39: 136–142. pmid:15009522
- View Article
- PubMed/NCBI
- Google Scholar
61. Watanabe K, Yilmaz Ö, Nakhjiri SF, Belton CM, Lamont RJ. Association of mitogen-activated protein kinase pathways with gingival epithelial cell responses to Porphyromonas gingivalis infection. Infect Immun. 2001; 69: 6731–6737. pmid:11598045
- View Article
- PubMed/NCBI
- Google Scholar
62. Dommisch H, Chung WO, Jepsen S, Hacker BM, Dale BA. PLC, p38/MAPK, and NF-κB-mediated induction of MIP-3α/CCL20 by Porphyromonas gingivalis. Innate Immun. 2010; 16: 226–234. pmid:19710093
- View Article
- PubMed/NCBI
- Google Scholar
63. Oboki K, Ohno T, Kajiwara N, Saito H, Nakae S. IL-33 and IL-33 receptors in host defense and diseases. Allergol Int. 2010; 59: 143–160. pmid:20414050
- View Article
- PubMed/NCBI
- Google Scholar
64. Snelgrove RJ, Gregory LG, Peiró T, Akthar S, Campbell GA, Walker SA, et al. Alternaria-derived serine protease activity drives IL-33 mediated asthma exacerbations. J Allergy Clin Immunol. 2014; 134: 583–592. pmid:24636086
- View Article
- PubMed/NCBI
- Google Scholar
65. Peyret-Lacombe A, Brunel G, Watts M, Charveron M, Duplan H. TLR2 sensing of F. nucleatum and S. sanguinis distinctly triggered gingival innate response. Cytokine. 2009; 46: 201–210. pmid:19299164
- View Article
- PubMed/NCBI
- Google Scholar
66. Cayrol C, Girard JP. The IL-1-like cytokine IL-33 is inactivated after maturation by caspase-1. Proc Natl Acad Sci U S A. 2009; 106: 9021–9026. pmid:19439663
- View Article
- PubMed/NCBI
- Google Scholar
67. Lüthi AU, Cullen SP, McNeela EA, Duriez PJ, Afonina IS, Sheridan C, et al. Suppression of interleukin-33 bioactivity through proteolysis by apoptotic caspases. Immunity. 2009; 31: 84–98. pmid:19559631
- View Article
- PubMed/NCBI
- Google Scholar
68. Beklen A, Tsaous Memet G. Interleukin-1 superfamily member, interleukin-33, in periodontal diseases. Biotech Histochem. 2013; 89: 209–214. pmid:24053163
- View Article
- PubMed/NCBI
- Google Scholar
69. Buduneli N, Özcaka Ö, Nalbantsoy A. Interleukin-33 levels in gingival crevicular fluid, saliva, or plasma do not differentiate chronic periodontitis. J Periodontol. 2012; 83: 362–368. pmid:21859321
- View Article
- PubMed/NCBI
- Google Scholar
70. Papathanasiou E, Teles F, Griffin T, Arguello E, Finkelman M, Hanley J, et al. Gingival crevicular fluid levels of interferon-γ, but not interleukin-4 or -33 or thymic stromal lymphopoietin, are increased in inflamed sites in patients with periodontal disease. J Periodontal Res. 2014; 49: 55–61. pmid:23550893
- View Article
- PubMed/NCBI
- Google Scholar
71. Calkins CC, Platt K, Potempa J, Travis J. Inactivation of tumor necrosis factor-α by proteinases (gingipains) from the periodontal pathogen, Porphyromonas gingivalis. J Biol Chem. 1998; 273: 6611–6614. pmid:9506956
- View Article
- PubMed/NCBI
- Google Scholar
72. Stathopoulou PG, Benakanakere MR, Galicia JC, Kinane DF. The host cytokine response to Porphyromonas gingivalis is modified by gingipains. Oral Microbiol Immunol. 2009; 24: 11–17. pmid:19121064
- View Article
- PubMed/NCBI
- Google Scholar
73. Delima AJ, Van Dyke TE. Origin and function of the cellular components in gingival crevice fluid. Periodontol 2000. 2003; 31: 55–76. pmid:12656996
- View Article
- PubMed/NCBI
- Google Scholar
74. Cayrol C, Girard JP. IL-33: an alarmin cytokine with crucial roles in innate immunity, inflammation and allergy. Curr Opin Immunol. 2014; 31: 31–37. pmid:25278425
- View Article
- PubMed/NCBI
- Google Scholar
75. Ali S, Mohs A, Thomas M, Klare J, Ross R, Schmitz ML, et al. The dual function cytokine IL-33 interacts with the transcription factor NF-κB to dampen NF- κB-stimulated gene transcription. J Immunol. 2011; 187: 1609–1616. pmid:21734074
- View Article
- PubMed/NCBI
- Google Scholar

[ref1] 1. Schmitz J, Owyang A, Oldham E, Song Y, Murphy E, McClanahan TK, et al. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity. 2015; 23: 479–490.
View Article
Google Scholar

[2] View Article

[3] Google Scholar

[ref2] 2. Moussion C, Ortega N, Girard JP. The IL-1-like cytokine IL-33 is constitutively expressed in the nucleus of endothelial cells and epithelial cells in vivo: a novel 'alarmin'? PLoS One. 2008; 3: e3331. pmid:18836528
View Article
PubMed/NCBI
Google Scholar

[5] View Article

[6] PubMed/NCBI

[7] Google Scholar

[ref3] 3. Saenz SA, Taylor BC, Artis D. Welcome to the neighborhood: epithelial cell-derived cytokines license innate and adaptive immune responses at mucosal sites. Immunol Rev. 2008; 226: 172–190. pmid:19161424
View Article
PubMed/NCBI
Google Scholar

[9] View Article

[10] PubMed/NCBI

[11] Google Scholar

[ref4] 4. Paul WE, Zhu J. How are T_H2-type immune responses initiated and amplified? Nat Rev Immunol. 2010; 10: 225–235. pmid:20336151
View Article
PubMed/NCBI
Google Scholar

[13] View Article

[14] PubMed/NCBI

[15] Google Scholar

[ref5] 5. Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT. Induction of IL-33 expression and activity in central nervous system glia. J Leukoc Biol. 2008; 84: 631–643. pmid:18552204
View Article
PubMed/NCBI
Google Scholar

[17] View Article

[18] PubMed/NCBI

[19] Google Scholar

[ref6] 6. Nile CJ, Barksby E, Jitprasertwong P, Preshaw PM, Taylor JJ. Expression and regulation of interleukin-33 in human monocytes. Immunology. 2010; 130: 172–180. pmid:20070408
View Article
PubMed/NCBI
Google Scholar

[21] View Article

[22] PubMed/NCBI

[23] Google Scholar

[ref7] 7. Shimosato T, Fujimoto M, Tohno M, Sato T, Tateo M, Otani H, et al. CpG oligodeoxynucleotides induce strong up-regulation of interleukin 33 via Toll-like receptor 9. Biochem Biophys Res Commun. 2010; 394: 81–86. pmid:20178773
View Article
PubMed/NCBI
Google Scholar

[25] View Article

[26] PubMed/NCBI

[27] Google Scholar

[ref8] 8. Meephansan J, Tsuda H, Komine M, Tominaga S, Ohtsuki M. Regulation of IL-33 expression by IFN-γ and tumor necrosis factor-α in normal human epidermal keratinocytes. J Invest Dermatol. 2012; 132: 2593–2600. pmid:22673732
View Article
PubMed/NCBI
Google Scholar

[29] View Article

[30] PubMed/NCBI

[31] Google Scholar

[ref9] 9. Le H, Kim W, Kim J, Cho HR, Kwon B. Interleukin-33: a mediator of inflammation targeting hematopoietic stem and progenitor cells and their progenies. Front Immunol. 2013; 4: 1–9. pmid:23653627
View Article
PubMed/NCBI
Google Scholar

[33] View Article

[34] PubMed/NCBI

[35] Google Scholar

[ref10] 10. Jones LA, Roberts F, Nickdel MB, Brombacher F, McKenzie ANJ, Henriquez FL, et al. IL-33 receptor (T1/ST2) signalling is necessary to prevent the development of encephalitis in mice infected with Toxoplasma gondii. Eur J Immunol. 2010; 40: 426–436. pmid:19950183
View Article
PubMed/NCBI
Google Scholar

[37] View Article

[38] PubMed/NCBI

[39] Google Scholar

[ref11] 11. Moro K, Yamada T, Tanabe M, Takeuchi T, Ikawa T, Kawamoto H, et al. Innate production of T_H2 cytokines by adipose tissue-associated c-Kit⁺Sca-1⁺ lymphoid cells. Nature. 2010; 463: 540–544. pmid:20023630
View Article
PubMed/NCBI
Google Scholar

[41] View Article

[42] PubMed/NCBI

[43] Google Scholar

[ref12] 12. Neill DR, Wong SH, Bellosi A, Flynn RJ, Daly M, Langford TKA, et al. Nuocytes represent a new innate effector leukocyte that mediates type-2 immunity. Nature. 2010; 464: 1367–1370. pmid:20200518
View Article
PubMed/NCBI
Google Scholar

[45] View Article

[46] PubMed/NCBI

[47] Google Scholar

[ref13] 13. Price AE, Liang HE, Sullivan BM, Reinhardt RL, Eisley CJ, Erle DJ, et al. Systemically dispersed innate IL-13-expressing cells in type 2 immunity. Proc Natl Acad Sci U S A. 2010; 107: 11489–11494. pmid:20534524
View Article
PubMed/NCBI
Google Scholar

[49] View Article

[50] PubMed/NCBI

[51] Google Scholar

[ref14] 14. Yasuda K, Muto T, Kawagoe T, Matsumoto M, Sasaki Y, Matsushita K, et al. Contribution of IL-33-activated type II innate lymphoid cells to pulmonary eosinophilia in intestinal nematode-infected mice. Proc Natl Acad Sci U S A. 2012; 109: 3451–3456. pmid:22331917
View Article
PubMed/NCBI
Google Scholar

[53] View Article

[54] PubMed/NCBI

[55] Google Scholar

[ref15] 15. Préfontaine D, Lajoie-Kadoch S, Foley S, Audusseau S, Olivenstein R, Halayko AJ, et al. Increased expression of IL-33 in severe asthma: evidence of expression by airway smooth muscle cells. J Immunol. 2009; 183: 5094–5103. pmid:19801525
View Article
PubMed/NCBI
Google Scholar

[57] View Article

[58] PubMed/NCBI

[59] Google Scholar

[ref16] 16. Haenuki Y, Matsushita K, Futatsugi-Yumikura S, Ishii KJ, Kawagoe T, Imoto Y, et al. A critical role of IL-33 in experimental allergic rhinitis. J Allergy Clin Immunol. 2012; 130: 184–194. pmid:22460070
View Article
PubMed/NCBI
Google Scholar

[61] View Article

[62] PubMed/NCBI

[63] Google Scholar

[ref17] 17. Byers DE, Alexander-Brett J, Patel AC, Agapov E, Dang-Vu G, Jin X, et al. Long-term IL-33-producing epithelial progenitor cells in chronic obstructive lung disease. J Clin Invest. 2013; 123: 3967–3982. pmid:23945235
View Article
PubMed/NCBI
Google Scholar

[65] View Article

[66] PubMed/NCBI

[67] Google Scholar

[ref18] 18. Schiering C, Krausgruber T, Chomka A, Fröhlich A, Adelmann K, Wohlfert EA, et al. The alarmin IL-33 promotes regulatory T-cell function in the intestine. Nature. 2014; 513: 564–568. pmid:25043027
View Article
PubMed/NCBI
Google Scholar

[69] View Article

[70] PubMed/NCBI

[71] Google Scholar

[ref19] 19. Chang YJ, Kim HY, Albacker LA, Baumgarth N, McKenzie ANJ, Smith DE, et al. Innate lymphoid cells mediate influenza-induced airway hyper-reactivity independently of adaptive immunity. Nat Immunol. 2011; 12: 631–638. pmid:21623379
View Article
PubMed/NCBI
Google Scholar

[73] View Article

[74] PubMed/NCBI

[75] Google Scholar

[ref20] 20. Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H. IL-33-responsive lineage^- CD25⁺ CD44^hi lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs. J Immunol. 2012; 188: 1503–1513. pmid:22198948
View Article
PubMed/NCBI
Google Scholar

[77] View Article

[78] PubMed/NCBI

[79] Google Scholar

[ref21] 21. Kim HY, Chang YJ, Subramanian S, Lee HH, Albacker LA, Matangkasombut P, et al. Innate lymphoid cells responding to IL-33 mediate airway hyperreactivity independently of adaptive immunity. J Allergy Clin Immunol. 2012; 129: 216–227. pmid:22119406
View Article
PubMed/NCBI
Google Scholar

[81] View Article

[82] PubMed/NCBI

[83] Google Scholar

[ref22] 22. Enoksson M, Lyberg K, Möller-Westerberg C, Fallon PG, Nilsson G, Lunderius-Andersson C. Mast cells as sensors of cell injury through IL-33 recognition. J Immunol. 2011; 186: 2523–2528. pmid:21239713
View Article
PubMed/NCBI
Google Scholar

[85] View Article

[86] PubMed/NCBI

[87] Google Scholar

[ref23] 23. Pathirana RD, O'Brien-Simpson NM, Reynolds EC. Host immune responses to Porphyromonas gingivalis antigens. Periodontol 2000. 2010; 52: 218–237. pmid:20017803
View Article
PubMed/NCBI
Google Scholar

[89] View Article

[90] PubMed/NCBI

[91] Google Scholar

[ref24] 24. Potempa J, Sroka A, Imamura T, Travis J. Gingipains, the major cysteine proteinases and virulence factors of Porphyromonas gingivalis: structure, function and assembly of multidomain protein complexes. Curr Protein Pept Sci. 2003; 4: 397–407. pmid:14683426
View Article
PubMed/NCBI
Google Scholar

[93] View Article

[94] PubMed/NCBI

[95] Google Scholar

[ref25] 25. Jagels MA, Travis J, Potempa J, Pike R, Hugli TE. Proteolytic inactivation of the leukocyte C5a receptor by proteinases derived from Porphyromonas gingivalis. Infect Immun. 1996; 64: 1984–1991. pmid:8675297
View Article
PubMed/NCBI
Google Scholar

[97] View Article

[98] PubMed/NCBI

[99] Google Scholar

[ref26] 26. Mikolajczyk-Pawlinska J, Travis J, Potempa J. Modulation of interleukin-8 activity by gingipains from Porphyromonas gingivalis: implications for pathogenicity of periodontal disease. FEBS Lett. 1998; 440: 282–286. pmid:9872387
View Article
PubMed/NCBI
Google Scholar

[101] View Article

[102] PubMed/NCBI

[103] Google Scholar

[ref27] 27. Yilmaz Ö, Verbeke P, Lamont RJ, Ojcius DM. Intercellular spreading of Porphyromonas gingivalis infection in primary gingival epithelial cells. Infect Immun. 2006; 74: 703–710. pmid:16369027
View Article
PubMed/NCBI
Google Scholar

[105] View Article

[106] PubMed/NCBI

[107] Google Scholar

[ref28] 28. Tada H, Shimauchi H, Takada H, Matsushita K. Possible roles of IL-33 in periodontal diseases: Porphyromonas gingivalis induced IL-33 in human gingival epithelial cells. In: Sasaki K, Suzuki O, Takahashi N, editors. Interface Oral Health Science. Tokyo: Springer Japan; 2015. pp. 293–303.

[ref29] 29. Malcolm J, Awang RA, Oliver-Bell J, Butcher JP, Campbell L, Adrados Planell A, et al. IL-33 exacerbates periodontal disease through induction of RANKL. J Dent Res. 2015; 94: 968–975. pmid:25808546
View Article
PubMed/NCBI
Google Scholar

[110] View Article

[111] PubMed/NCBI

[112] Google Scholar

[ref30] 30. Ogawa T, Shimauchi H, Hamada S. Mucosal and systemic immune responses in BALB/c mice to Bacteroides gingivalis fimbriae administered orally. Infect Immun. 1989; 57: 3466–3471. pmid:2572554
View Article
PubMed/NCBI
Google Scholar

[114] View Article

[115] PubMed/NCBI

[116] Google Scholar

[ref31] 31. Ogawa T, Asai Y, Hashimoto M, Uchida H. Bacterial fimbriae activate human peripheral blood monocytes utilizing TLR2, CD14 and CD11a/CD18 as cellular receptors. Eur J Immunol. 2002; 32: 2543–2550. pmid:12207338
View Article
PubMed/NCBI
Google Scholar

[118] View Article

[119] PubMed/NCBI

[120] Google Scholar

[ref32] 32. Makimura Y, Asai Y, Taiji Y, Sugiyama A, Tamai R, Ogawa T. Correlation between chemical structure and biological activities of Porphyromonas gingivalis synthetic lipopeptide derivatives. Clin Exp Immunol. 2006; 146: 159–168. pmid:16968410
View Article
PubMed/NCBI
Google Scholar

[122] View Article

[123] PubMed/NCBI

[124] Google Scholar

[ref33] 33. Darveau RP, Pham TT, Lemley K, Reife RA, Bainbridge BW, Coats SR, et al. Porphyromonas gingivalis lipopolysaccharide contains multiple lipid A species that functionally interact with both Toll-like receptors 2 and 4. Infect Immun. 2004; 72: 5041–5051. pmid:15321997
View Article
PubMed/NCBI
Google Scholar

[126] View Article

[127] PubMed/NCBI

[128] Google Scholar

[ref34] 34. Shi Y, Ratnayake DB, Okamoto K, Abe N, Yamamoto K, Nakayama K. Genetic analyses of proteolysis, hemoglobin binding, and hemagglutination of Porphyromonas gingivalis. J Biol Chem. 1999; 274: 17955–17960. pmid:10364243
View Article
PubMed/NCBI
Google Scholar

[130] View Article

[131] PubMed/NCBI

[132] Google Scholar

[ref35] 35. Hashimoto M, Asai Y, Ogawa T. Separation and structural analysis of lipoprotein in a lipopolysaccharide preparation from Porphyromonas gingivalis. Int Immunol. 2004; 16: 1431–1437. pmid:15326096
View Article
PubMed/NCBI
Google Scholar

[134] View Article

[135] PubMed/NCBI

[136] Google Scholar

[ref36] 36. Kouzaki H, Iijima K, Kobayashi T, O'Grady SM, Kita H. The danger signal, extracellular ATP, is a sensor for an airborne allergen and triggers IL-33 release and innate Th2-type responses. J Immunol. 2011; 186: 4375–4387. pmid:21357533
View Article
PubMed/NCBI
Google Scholar

[138] View Article

[139] PubMed/NCBI

[140] Google Scholar

[ref37] 37. Kakkar R, Hei H, Dobner S, Lee RT. Interleukin 33 as a mechanically responsive cytokine secreted by living cells. J Biol Chem. 2012; 287: 6941–6948. pmid:22215666
View Article
PubMed/NCBI
Google Scholar

[142] View Article

[143] PubMed/NCBI

[144] Google Scholar

[ref38] 38. Rothmeier AS, Ruf W. Protease-activated receptor 2 signaling in inflammation. Semin Immunopathol. 2012; 34: 133–149. pmid:21971685
View Article
PubMed/NCBI
Google Scholar

[146] View Article

[147] PubMed/NCBI

[148] Google Scholar

[ref39] 39. Lourbakos A, Potempa J, Travis J, D'Andrea MR, Andrade-Gordon P, Santulli R, et al. Arginine-specific protease from Porphyromonas gingivalis activates protease-activated receptors on human oral epithelial cells and induces interleukin-6 secretion. Infect Immun. 2001; 69: 5121–5130. pmid:11447194
View Article
PubMed/NCBI
Google Scholar

[150] View Article

[151] PubMed/NCBI

[152] Google Scholar

[ref40] 40. Giacaman RA, Asrani AC, Ross KF, Herzberg MC. Cleavage of protease-activated receptors on an immortalized oral epithelial cell line by Porphyromonas gingivalis gingipains. Microbiology. 2009; 155: 3238–3246. pmid:19608609
View Article
PubMed/NCBI
Google Scholar

[154] View Article

[155] PubMed/NCBI

[156] Google Scholar

[ref41] 41. Böhm SK, Khitin LM, Grady EF, Aponte G, Payan DG, Bunnett NW. Mechanisms of desensitization and resensitization of proteinase-activated receptor-2. J Biol Chem. 1996; 271: 22003–22016. pmid:8703006
View Article
PubMed/NCBI
Google Scholar

[158] View Article

[159] PubMed/NCBI

[160] Google Scholar

[ref42] 42. Böhm SK, Kong W, Brömme D, Smeekens SP, Anderson DC, Connolly A, et al. Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2. Biochem J. 1996; 314: 1009–1016. pmid:8615752
View Article
PubMed/NCBI
Google Scholar

[162] View Article

[163] PubMed/NCBI

[164] Google Scholar

[ref43] 43. Seatter MJ, Drummond R, Kanke T, Macfarlane SR, Hollenberg MD, Plevin R. The role of the C-terminal tail in protease-activated receptor-2-mediated Ca²⁺ signalling, proline-rich tyrosine kinase-2 activation, and mitogen-activated protein kinase activity. Cell Signal. 2004; 16: 21–29. pmid:14607272
View Article
PubMed/NCBI
Google Scholar

[166] View Article

[167] PubMed/NCBI

[168] Google Scholar

[ref44] 44. Belham CM, Tate RJ, Scott PH, Pemberton AD, Miller HR, Wadsworth RM, et al. Trypsin stimulates proteinase-activated receptor-2-dependent and -independent activation of mitogen-activated protein kinases. Biochem J. 1996; 320: 939–946. pmid:9003384
View Article
PubMed/NCBI
Google Scholar

[170] View Article

[171] PubMed/NCBI

[172] Google Scholar

[ref45] 45. Macfarlane SR, Sloss CM, Cameron P, Kanke T, McKenzie RC, Plevin R. The role of intracellular Ca²⁺ in the regulation of proteinase-activated receptor-2 mediated nuclear factor kappa B signalling in keratinocytes. Br J Pharmacol. 2005; 145: 535–544. pmid:15821758
View Article
PubMed/NCBI
Google Scholar

[174] View Article

[175] PubMed/NCBI

[176] Google Scholar

[ref46] 46. Holzhausen M, Cortelli JR, da Silva VA, Franco GC, Cortelli SC, Vergnolle N. Protease-activated receptor-2 (PAR₂) in human periodontitis. J Dent Res. 2010; 89: 948–953. pmid:20530726
View Article
PubMed/NCBI
Google Scholar

[178] View Article

[179] PubMed/NCBI

[180] Google Scholar

[ref47] 47. Fagundes JA, Monoo LD, Euzébio Alves VT, Pannuti CM, Cortelli SC, Cortelli JR, et al. Porphyromonas gingivalis is associated with protease-activated receptor-2 upregulation in chronic periodontitis. J Periodontol. 2011; 82: 1596–1601. pmid:21513479
View Article
PubMed/NCBI
Google Scholar

[182] View Article

[183] PubMed/NCBI

[184] Google Scholar

[ref48] 48. Euzebio Alves VT, Bueno da Silva HA, de França BN, Eichler RS, Saraiva L, Catalli de Carvalho MH, et al. Periodontal treatment downregulates protease-activated receptor 2 in human gingival crevicular fluid cells. Infect Immun. 2013; 81: 4399–4407. pmid:24042113
View Article
PubMed/NCBI
Google Scholar

[186] View Article

[187] PubMed/NCBI

[188] Google Scholar

[ref49] 49. Dommisch H, Chung WO, Rohani MG, Williams D, Rangarajan M, Curtis MA, et al. Protease-activated receptor 2 mediates human beta-defensin 2 and CC chemokine ligand 20 mRNA expression in response to proteases secreted by Porphyromonas gingivalis. Infect Immun. 2007; 75: 4326–4333. pmid:17591792
View Article
PubMed/NCBI
Google Scholar

[190] View Article

[191] PubMed/NCBI

[192] Google Scholar

[ref50] 50. Kouzaki H, O'Grady SM, Lawrence CB, Kita H. Proteases induce production of thymic stromal lymphopoietin by airway epithelial cells through protease-activated receptor-2. J Immunol. 2009; 183: 1427–1434. pmid:19561109
View Article
PubMed/NCBI
Google Scholar

[194] View Article

[195] PubMed/NCBI

[196] Google Scholar

[ref51] 51. Kouzaki H, Tojima I, Kita H, Shimizu T. Transcription of interleukin-25 and extracellular release of the protein is regulated by allergen proteases in airway epithelial cells. Am J Respir Cell Mol Biol. 2013; 49: 741–750. pmid:23590308
View Article
PubMed/NCBI
Google Scholar

[198] View Article

[199] PubMed/NCBI

[200] Google Scholar

[ref52] 52. Allakhverdi Z, Smith DE, Comeau MR, Delespesse G. The ST2 ligand IL-33 potently activates and drives maturation of human mast cells. J Immunol. 2007; 179: 2051–2054. pmid:17675461
View Article
PubMed/NCBI
Google Scholar

[202] View Article

[203] PubMed/NCBI

[204] Google Scholar

[ref53] 53. DeFea KA, Zalevsky J, Thoma MS, Déry O, Mullins RD, Bunnett NW. β-arrestin-dependent endocytosis of proteinase-activated receptor 2 is required for intracellular targeting of activated ERK1/2. J Cell Biol. 2000; 148: 1267–1281. pmid:10725339
View Article
PubMed/NCBI
Google Scholar

[206] View Article

[207] PubMed/NCBI

[208] Google Scholar

[ref54] 54. Ricks TK, Trejo J. Phosphorylation of protease-activated receptor-2 differentially regulates desensitization and internalization. J Biol Chem. 2009; 284: 34444–34457. pmid:19815543
View Article
PubMed/NCBI
Google Scholar

[210] View Article

[211] PubMed/NCBI

[212] Google Scholar

[ref55] 55. Lamont RJ, Chan A, Belton CM, Izutsu KT, Vasel D, Weinberg A. Porphyromonas gingivalis invasion of gingival epithelial cells. Infect Immun. 1995; 63: 3878–3885. pmid:7558295
View Article
PubMed/NCBI
Google Scholar

[214] View Article

[215] PubMed/NCBI

[216] Google Scholar

[ref56] 56. Boisvert H, Duncan MJ. Clathrin-dependent entry of a gingipain adhesin peptide and Porphyromonas gingivalis into host cells. Cell Microbiol. 2008; 10: 2538–2552. pmid:18717820
View Article
PubMed/NCBI
Google Scholar

[218] View Article

[219] PubMed/NCBI

[220] Google Scholar

[ref57] 57. Pike RN, Potempa J, McGraw W, Coetzer THT, Travis J. Characterization of the binding activities of proteinase-adhesin complexes from Porphyromonas gingivalis. J Bacteriol. 1996; 178: 2876–2882. pmid:8631676
View Article
PubMed/NCBI
Google Scholar

[222] View Article

[223] PubMed/NCBI

[224] Google Scholar

[ref58] 58. Nakayama K, Yoshimura F, Kadowaki T, Yamamoto K. Involvement of arginine-specific cysteine proteinase (Arg-gingipain) in fimbriation of Porphyromonas gingivalis. J Bacteriol. 1996; 178: 2818–2824. pmid:8631669
View Article
PubMed/NCBI
Google Scholar

[226] View Article

[227] PubMed/NCBI

[228] Google Scholar

[ref59] 59. Kato T, Tsuda T, Omori H, Kato T, Yoshimori T, Amano A. Maturation of fimbria precursor protein by exogenous gingipains in Porphyromonas gingivalis gingipain-null mutant. FEMS Microbiol Lett. 2007; 273: 96–102. pmid:17559394
View Article
PubMed/NCBI
Google Scholar

[230] View Article

[231] PubMed/NCBI

[232] Google Scholar

[ref60] 60. Amano A, Nakagawa I, Okahashi N, Hamada N. Variations of Porphyromonas gingivalis fimbriae in relation to microbial pathogenesis. J Periodontal Res. 2004; 39: 136–142. pmid:15009522
View Article
PubMed/NCBI
Google Scholar

[234] View Article

[235] PubMed/NCBI

[236] Google Scholar

[ref61] 61. Watanabe K, Yilmaz Ö, Nakhjiri SF, Belton CM, Lamont RJ. Association of mitogen-activated protein kinase pathways with gingival epithelial cell responses to Porphyromonas gingivalis infection. Infect Immun. 2001; 69: 6731–6737. pmid:11598045
View Article
PubMed/NCBI
Google Scholar

[238] View Article

[239] PubMed/NCBI

[240] Google Scholar

[ref62] 62. Dommisch H, Chung WO, Jepsen S, Hacker BM, Dale BA. PLC, p38/MAPK, and NF-κB-mediated induction of MIP-3α/CCL20 by Porphyromonas gingivalis. Innate Immun. 2010; 16: 226–234. pmid:19710093
View Article
PubMed/NCBI
Google Scholar

[242] View Article

[243] PubMed/NCBI

[244] Google Scholar

[ref63] 63. Oboki K, Ohno T, Kajiwara N, Saito H, Nakae S. IL-33 and IL-33 receptors in host defense and diseases. Allergol Int. 2010; 59: 143–160. pmid:20414050
View Article
PubMed/NCBI
Google Scholar

[246] View Article

[247] PubMed/NCBI

[248] Google Scholar

[ref64] 64. Snelgrove RJ, Gregory LG, Peiró T, Akthar S, Campbell GA, Walker SA, et al. Alternaria-derived serine protease activity drives IL-33 mediated asthma exacerbations. J Allergy Clin Immunol. 2014; 134: 583–592. pmid:24636086
View Article
PubMed/NCBI
Google Scholar

[250] View Article

[251] PubMed/NCBI

[252] Google Scholar

[ref65] 65. Peyret-Lacombe A, Brunel G, Watts M, Charveron M, Duplan H. TLR2 sensing of F. nucleatum and S. sanguinis distinctly triggered gingival innate response. Cytokine. 2009; 46: 201–210. pmid:19299164
View Article
PubMed/NCBI
Google Scholar

[254] View Article

[255] PubMed/NCBI

[256] Google Scholar

[ref66] 66. Cayrol C, Girard JP. The IL-1-like cytokine IL-33 is inactivated after maturation by caspase-1. Proc Natl Acad Sci U S A. 2009; 106: 9021–9026. pmid:19439663
View Article
PubMed/NCBI
Google Scholar

[258] View Article

[259] PubMed/NCBI

[260] Google Scholar

[ref67] 67. Lüthi AU, Cullen SP, McNeela EA, Duriez PJ, Afonina IS, Sheridan C, et al. Suppression of interleukin-33 bioactivity through proteolysis by apoptotic caspases. Immunity. 2009; 31: 84–98. pmid:19559631
View Article
PubMed/NCBI
Google Scholar

[262] View Article

[263] PubMed/NCBI

[264] Google Scholar

[ref68] 68. Beklen A, Tsaous Memet G. Interleukin-1 superfamily member, interleukin-33, in periodontal diseases. Biotech Histochem. 2013; 89: 209–214. pmid:24053163
View Article
PubMed/NCBI
Google Scholar

[266] View Article

[267] PubMed/NCBI

[268] Google Scholar

[ref69] 69. Buduneli N, Özcaka Ö, Nalbantsoy A. Interleukin-33 levels in gingival crevicular fluid, saliva, or plasma do not differentiate chronic periodontitis. J Periodontol. 2012; 83: 362–368. pmid:21859321
View Article
PubMed/NCBI
Google Scholar

[270] View Article

[271] PubMed/NCBI

[272] Google Scholar

[ref70] 70. Papathanasiou E, Teles F, Griffin T, Arguello E, Finkelman M, Hanley J, et al. Gingival crevicular fluid levels of interferon-γ, but not interleukin-4 or -33 or thymic stromal lymphopoietin, are increased in inflamed sites in patients with periodontal disease. J Periodontal Res. 2014; 49: 55–61. pmid:23550893
View Article
PubMed/NCBI
Google Scholar

[274] View Article

[275] PubMed/NCBI

[276] Google Scholar

[ref71] 71. Calkins CC, Platt K, Potempa J, Travis J. Inactivation of tumor necrosis factor-α by proteinases (gingipains) from the periodontal pathogen, Porphyromonas gingivalis. J Biol Chem. 1998; 273: 6611–6614. pmid:9506956
View Article
PubMed/NCBI
Google Scholar

[278] View Article

[279] PubMed/NCBI

[280] Google Scholar

[ref72] 72. Stathopoulou PG, Benakanakere MR, Galicia JC, Kinane DF. The host cytokine response to Porphyromonas gingivalis is modified by gingipains. Oral Microbiol Immunol. 2009; 24: 11–17. pmid:19121064
View Article
PubMed/NCBI
Google Scholar

[282] View Article

[283] PubMed/NCBI

[284] Google Scholar

[ref73] 73. Delima AJ, Van Dyke TE. Origin and function of the cellular components in gingival crevice fluid. Periodontol 2000. 2003; 31: 55–76. pmid:12656996
View Article
PubMed/NCBI
Google Scholar

[286] View Article

[287] PubMed/NCBI

[288] Google Scholar

[ref74] 74. Cayrol C, Girard JP. IL-33: an alarmin cytokine with crucial roles in innate immunity, inflammation and allergy. Curr Opin Immunol. 2014; 31: 31–37. pmid:25278425
View Article
PubMed/NCBI
Google Scholar

[290] View Article

[291] PubMed/NCBI

[292] Google Scholar

[ref75] 75. Ali S, Mohs A, Thomas M, Klare J, Ross R, Schmitz ML, et al. The dual function cytokine IL-33 interacts with the transcription factor NF-κB to dampen NF- κB-stimulated gene transcription. J Immunol. 2011; 187: 1609–1616. pmid:21734074
View Article
PubMed/NCBI
Google Scholar

[294] View Article

[295] PubMed/NCBI

[296] Google Scholar

Figures

Abstract

Introduction

Materials and Methods

Ethics statement

Reagents

Culture of P. gingivalis and preparation of whole cell preparations

Cell and cell lines

IL-33 detection by immunohistochemistry

Reverse transcription and real-time polymerase chain reaction (RT-qPCR)

Western blotting

IL-33 detection by immunofluorescence assay

RNA interference

NF-κB luciferase assay

Measurement of IL-33 production by ELISA

Enzyme activity assay

Statistical analysis

Results

Expression of IL-33 in human periodontal tissues

P. gingivalis increases IL-33 mRNA expression in human gingival epithelial cells

P. gingivalis increases IL-33 protein expression

Gingipains participate in increased expression of IL-33 mRNA induced by P. gingivalis

Involvement of PAR-2 and PLC in IL-33 induction by gingipains

Involvement of p38 and NF-κB in IL-33 induction by gingipains

Discussion

Supporting Information

S1 Fig. P. gingivalis increases IL-25 and TSLP mRNA expressions in gingival epithelial cells.

S2 Fig. Nuclear expression of IL-33 in resting gingival epithelial cells.

S3 Fig. Amidolytic activities of various gingipain-mutant strains of whole P. gingivalis cells.

S4 Fig. Involvement of gingipains in P. gingivalis-induced IL-25 and TSLP mRNA expression in gingival epithelial cells.

S5 Fig. P. gingivalis-induced IL-33 mRNA expression partially requires expression of β-arrestin-1.

S6 Fig. Cell viability and cytotoxicity of gingival epithelial cells after stimulation with P. gingivalis.

Acknowledgments

Author Contributions

References