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Open Access

Correction

Correction: Oral Delivery of a Novel Recombinant Streptococcus mitis Vector Elicits Robust Vaccine Antigen-Specific Oral Mucosal and Systemic Antibody Responses and T Cell Tolerance

  • Emily Xie,
  • Abhiroop Kotha,
  • Tracy Biaco,
  • Nikita Sedani,
  • Jonathan Zou,
  • Phillip Stashenko,
  • Margaret J. Duncan,
  • Antonio Campos-Neto,
  • Mark J. Cayabyab

Fig 2 is incorrect. Panel D is missing. The authors have provided a corrected version here.

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Fig 2. Recombinant S. mitis expresses HIV envelope protein.

rS. mitis with the integrated HIV HXBc2 Env gp120 was designed to secrete HIV Env by ligating the HIV Env in frame with 250bp 5’ end of the pullulanase gene (pulA/Smt0163) encoding a signal peptide that allows processing and secretion of the HIV antigen. (A) The signal peptide has an amino-terminal region (N), a hydrophobic core (H), a signal peptidase cleavage site (C), and an accessory Sec transport motif (AST). Expression of HIV Env containing a C-terminal His tag was assessed by Western blotting using Penta-His-HRP from a representative recombinant clone in S. mitis lysates (B) and in culture supernatants (C) by TCA-precipitation (TCA), acetone precipitation (Acetone) and Amicon filter-concentration (Sup). HIV Env expression in lysates (B) and supernatants of control S. mitis vector (control) (C) is shown. The arrow denotes expression of the Env Ag band. 100 ng of His-tagged M. tuberculosis protein (MT0401) was used as a positive control (B and C, lane 1). (D) The expression of HIV-1 gp120 in rS. mitis containing the HIV Env gene (lane 2) in Amicon filter-concentrated supernatant was detected using human HIV patient sera. rS. mitis containing the empty plasmid was used as a negative control (lane 1).

https://doi.org/10.1371/journal.pone.0147781.g001

Reference

  1. 1. Xie E, Kotha A, Biaco T, Sedani N, Zou J, Stashenko P, et al. (2015) Oral Delivery of a Novel Recombinant Streptococcus mitis Vector Elicits Robust Vaccine Antigen-Specific Oral Mucosal and Systemic Antibody Responses and T Cell Tolerance. PLoS ONE 10(11): e0143422. pmid:26618634

Citation: Xie E, Kotha A, Biaco T, Sedani N, Zou J, Stashenko P, et al. (2016) Correction: Oral Delivery of a Novel Recombinant Streptococcus mitis Vector Elicits Robust Vaccine Antigen-Specific Oral Mucosal and Systemic Antibody Responses and T Cell Tolerance. PLoS ONE 11(1): e0147781. https://doi.org/10.1371/journal.pone.0147781

Published: January 22, 2016

Copyright: © 2016 Xie et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.