Figures
Abstract
Background
Maternal smoking has been associated with elevated risk of type 2 diabetes among the offspring in adulthood. The mechanisms underlying this fetal “programming” effect remain unclear. The present study sought to explore whether maternal smoking affects metabolic health biomarkers in fetuses/newborns.
Methods
In a prospective singleton pregnancy cohort (n = 248), we compared metabolic health biomarkers in the newborns of smoking and non-smoking mothers. Outcomes included cord plasma insulin, proinsulin, insulin-like growth factor I (IGF-I), IGF-II, leptin and adiponectin concentrations, glucose-to-insulin ratio (an indicator of insulin sensitivity) and proinsulin-to-insulin ratio (an indicator of β-cell function).
Results
Independent of maternal (glucose tolerance, age, ethnicity, parity, education, body mass index, alcohol use) and infant (sex, gestational age, birth weight z score, mode of delivery, cord blood glucose concentration) characteristics, the newborns of smoking mothers had lower IGF-I concentrations (mean: 6.7 vs. 8.4 nmol/L, adjusted p = 0.006), and marginally higher proinsulin-to-insulin ratios (0.94 vs. 0.72, adjusted p = 0.06) than the newborns of non-smoking mothers. Cord plasma insulin, proinsulin, IGF-II, leptin and adiponectin concentrations and glucose-to-insulin ratios were similar in the newborns of smoking and non-smoking mothers.
Conclusions
Maternal smoking was associated with decreased fetal IGF-I levels, and borderline lower fetal β-cell function. Larger cohort studies are required to confirm the latter finding. The preliminary findings prompt the hypothesis that these early life metabolic changes may be involved in the impact of maternal smoking on future risk of metabolic syndrome related disorders in the offspring.
Citation: Fang F, Luo Z-C, Dejemli A, Delvin E, Zhang J (2015) Maternal Smoking and Metabolic Health Biomarkers in Newborns. PLoS ONE 10(11): e0143660. https://doi.org/10.1371/journal.pone.0143660
Editor: Yael Abreu-Villaça, Universidade do Estado do Rio de Janeiro, BRAZIL
Received: August 8, 2015; Accepted: November 6, 2015; Published: November 23, 2015
Copyright: © 2015 Fang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: All relevant data are contained within the paper.
Funding: This work was supported by research grants from the Canadian Institutes of Health Research (CIHR grant # 79896) and National Natural Science Foundation of China (grant # 81370742). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
Cigarette smoking is a major public health problem, and constitutes a particular risk to the fetus due to the special vulnerability during developmental stage [1]. Smoking is common in pregnant women in many countries [2, 3]. An adverse intrauterine environment may have a lasting effect on chronic disease susceptibility in adulthood (Barker’s hypothesis) [4]. Epidemiological studies have demonstrated a strong association between maternal smoking and an increased risk of type 2 diabetes among the offspring in adulthood [5, 6]. Maternal smoking is a major risk factor of intrauterine growth restriction (IUGR) [7, 8] which itself is associated with an increased risk of type 2 diabetes in adulthood [9]. It is unclear whether maternal smoking affects metabolic health in utero directly leading to an increased susceptibility to type 2 diabetes in adulthood, or the effect may be solely mediated by its impact on fetal growth. So far, the mechanisms leading to elevated risk of type 2 diabetes mellitus in adulthood following in utero exposure to tobacco remain largely unknown. Studies in animal models suggest that smoking may affect metabolic health status in fetal or early postnatal life including impaired glucose tolerance, decreased insulin sensitivity [10], decreased pancreatic islet size and number [11]. Fetal and neonatal exposure to nicotine—a major constituent of cigarette smoke, may impair glucose homeostasis [12] and enhance the susceptibility to metabolic syndrome in rat offspring [13]. However, there is a lack of data on the impact of maternal smoking on metabolic health in human fetuses or newborns. The present study aimed to explore this question.
Methods
Study population
The present study was based on a prospective pregnancy cohort that was described previously [14], but addressed a new research question and did not overlap with previously reported data. Briefly, a total of 339 singleton pregnant women at three obstetric care centers in Montreal were recruited between August 2006 and December 2008. Data and specimens were collected at 24–28 and 32–35 weeks of gestation and delivery. Data on maternal and pregnancy characteristics were collected using structured study questionnaires through face-to-face interviews and medical chart reviews by trained research nurses and assistants. A total of 248 mother-infant pairs (73%) with complete data on maternal smoking and all studied cord blood biomarkers constituted the final study cohort.
Ethics statement
The study was approved by the research ethics committee of Sainte-Justine Hospital Research Centre, University of Montreal. Written informed consent was obtained from all study participants, according to the protocol approved by the research ethics committee.
Maternal smoking
Maternal smoking during pregnancy was based on self-reports. The women were asked to provide information on smoking at 24–28 and 32–35 weeks of gestation: did you smoke cigarette in the last three months? If the mother answered “yes” at either time point, she was defined as a smoker during pregnancy. According to this criterion, 18 were smokers (17 reported smoking at both gestational age windows) and 230 were non-smokers in the study cohort. For smokers, we further asked a question “on average, how many cigarettes you smoked per day” at both gestational age windows.
Anthropometric measurements
Maternal pre-pregnancy BMI was calculated as self-reported weight in kilogram divided by the square of measured height in meter. Birth weight was measured to the nearest gram using the routinely available electronic weighing device in each delivery unit, and z-score was calculated based on sex- and gestational age-specific Canadian fetal growth standards [15].
Specimens and biochemical assays
As reported previously [14], maternal blood specimens were collected at 24–28 weeks of gestation in the routine prenatal 50-g 1-h oral glucose tolerance test (OGTT) to screen for gestational diabetes mellitus. Maternal blood glucose concentrations were taken from routine prenatal clinical test records. Venous cord blood specimens were collected immediately after the delivery of the baby. All specimens collected were kept in a freezer at -80°C until assays.
Plasma glucose, insulin and proinsulin concentrations were determined by automated glucose oxidase method, automated ultrasensitive chemiluminescent immunometric assay (DXi 800, Beckman coulter, California) and quantitative enzyme-linked immuno sorbent assay (ELISA) kit, respectively. Cord plasma glucose/insulin ratio and proinsulin concentration were used as surrogate indicators of fetal insulin sensitivity, and proinsulin-to-insulin ratio as a surrogate indicator of β-cell function [14]. Leptin and total adiponectin were measured by a human leptin immunoassay kit and human adiponectin immunoassay kit, respectively [16]. Total IGF-I and total IGF-II were measured by an automated solid-phase, enzyme-labeled chemiluminescent assay (IMMULITE 2000 Siemens) and a human IGF-II ELISA kit respectively [17]. The intra-assay and inter-assay coefficients of variation of these assays were in the range of 2%-10%. The detection limits were 3.3 pmol/L for insulin, 2.0 pmol/L for proinsulin, 0.1 μg/L for leptin, 0.1 mg/L for adiponectin, 3.2 nmol/L for IGF-I and 2.0 nmol/L for IGF-II, respectively.
Statistical analysis
All data analyses were carried out using SAS version 9.2 (SAS Institute Inc, Cary, NC). Frequencies for categorical variables and mean/ standard deviation (SD) and median for continuous variables were calculated. Chi-square, Student’s t (for maternal age, birth weight) and Wilcoxon tests (for continuous variables with skewed data distribution) were used to compare maternal and newborn’s characteristics between smokers and non-smokers. Spearman partial correlation coefficients were calculated to evaluate the associations between the number of cigarettes smoked per day and fetal metabolic health biomarkers adjusting for gestational age. Log transformation was applied to metabolic health biomarkers (positively skewed data distribution) in generalized linear models to assess the differences between smokers and non-smokers adjusting for multiple categorical and continuous covariates. The covariates included maternal glucose tolerance (50g OGTT glucose concentration), pre-pregnancy BMI, ethnicity (Caucasian: yes or no), education (university: yes or no), age (<20, 20–34, > = 35 y), parity (primiparous: yes or no), alcohol use (yes or no), infant sex, birth weight (z score), gestational age (weeks), mode of delivery (caesarean or vaginal) and cord blood glucose concentration. A two-tailed probability (p) value of <0.05 was considered statistically significant.
Results
Maternal and newborn’s characteristics
Comparing maternal smokers vs. non-smokers, there were no significant differences in pre-pregnancy BMI, age, parity, and newborn’s sex, mode of delivery and gestational age (Table 1). Smokers were more likely to be a Caucasian and to drink alcohol, but less likely to have completed university education. The difference in birth weight was on the borderline of statistical significance (mean: 3253 vs. 3459 g in the newborns of smokers vs. non-smokers, p = 0.057). Birth weight z scores were lower in smokers but the difference was not statistically significant (smoker -0.10 vs. non-smoker 0.16, p = 0.255). There were no significant differences in maternal OGTT and cord blood glucose levels in the neonates of smokers vs. non-smokers.
Among the 18 smokers, based on self-reports at 24–28 weeks and 32–35 weeks of gestation, the median (interquartile range) number of cigarettes smoked per day during mid and late gestation was 5 (1–8). There were 8 women smoked 1–4 cigarettes/day, 6 women smoked 5–9 cigarettes/day, and 4 women smoked 10–15 cigarettes/day.
Fetal metabolic health biomarkers
Partial correlations adjusting for gestational age at birth showed that the average number of self-reported cigarettes smoked per day during mid and late gestation was positively correlated to cord plasma glucose-to-insulin ratio (r = 0.154, p = 0.017), proinsulin-to-insulin ratio (r = 0.152, p = 0.018), and negatively correlated to cord plasma insulin (r = -0.131, p = 0.041) and IGF-1 (r = -0.157, p = 0.014) concentrations, but not correlated to proinsulin, IGF-II, leptin and adiponectin concentrations (all p>0.5). The average number of cigarettes smoked per day reported at 24–28 weeks and 32–35 weeks of gestation had similar correlations with fetal metabolic health biomarkers (data not shown).
The crude comparisons showed that the neonates of smoking mothers had higher cord blood glucose-to-insulin ratios (mean: 34.9 vs. 24.4, p = 0.038), lower IGF-I concentrations (mean: 6.7 vs. 8.4 pmol/L, p = 0.05) and marginally higher proinsulin-to-insulin ratios (mean: 0.94 vs.0.72, p = 0.06) than the neonates of smoking mothers (Table 2). After the adjustments for maternal (glucose tolerance, pre-pregnancy BMI, ethnicity, educational, age, parity, alcohol use) and newborn’s (sex, gestational age, birth weight z score, mode of delivery and cord blood glucose concentration) characteristics, the difference in glucose-to-insulin ratio between smokers and non-smokers became non-significant (adjusted p = 0.14), the difference in IGF-I concentrations became more statistically significant (adjusted p = 0.006), while the difference in proinsulin-to-insulin ratio was on the borderline of statistical significance (adjusted P = 0.06). There were no significant differences (crude or adjusted) in cord blood insulin, proinsulin, IGF-II, leptin and adiponectin concentrations between the newborns of smokers and non-smokers.
Maternal metabolic health biomarkers
Comparing smokers vs. non-smokers, there were no significant differences in maternal plasma insulin, proinsulin, IGF-I, leptin and adiponectin concentrations in maternal 50 g OGTT blood at 24–28 weeks’ gestation (Table 2). Surprisingly, there were significantly lower maternal plasma IGF-II concentrations in smokers after the adjustment for glucose tolerance, pre-pregnancy BMI, ethnicity, age, education, parity and alcohol use.
Discussion
Main findings
To our knowledge, this is the first study exploring the possible impact of maternal smoking on fetal β-cell function in humans. Our data provide important preliminary evidence indicating that maternal smoking may decrease fetal IGF-I levels, and may also impair β-cell function, although the latter association is on the borderline of statistical significance and requires confirmation in larger studies. The new findings prompt the hypothesis that these effects in early life may be a mechanism in “programming” the long-term impact of maternal smoking on metabolic health in the offspring.
Data interpretation
IGF-I and IGF-II.
IGF-I is synthesized largely in the liver but also in other tissues. Classically, IGF-I is a key driver of fetal growth [18]. However, studies in mouse models and cultured insulinoma cells suggest that IGF-I also plays an important role in maintaining β-cell function by mediating IRS-2/PI3K/Akt pathway [19,20]. There have been inconsistent findings concerning whether IGF-I levels are altered in the newborns of smoking mothers, while there is a scarcity of data on IGF-II levels. We observed that cord blood plasma IGF-I but not IGF-II levels were decreased in the newborns of smoking mothers independent of fetal growth (birth weight z score). Consistent with our findings, Chelchowska and colleagues [21] studied 50 healthy pregnant women and found that cord blood IGF-I concentrations were lower (by 20%) in the newborns of smoking mothers. However, their analyses did not adjust for fetal growth. Similar to our findings, Pringle et al [22] observed that cord plasma IGF-I concentrations were lower in the babies of mothers who smoked, but IGF-II concentrations were unaffected by smoking status. In contrast, Ermis et al [23] analyzed 44 mother-infant (7-days old) pairs and observed no significant difference in IGF-I concentrations in infant serum samples according to maternal smoking status. The reasons could be due to small sample size and difference in the timing of IGF-I detection (at birth vs. after birth). Insulin is the primary driver of fetal IGF-I production [24]. We observed lower cord plasma insulin levels in the newborns of smoking mothers, but the difference did not reach statistical significance. Similarly, lower cord blood insulin and IGF-I levels have been reported in the newborns of smoking mothers [25].
Unexpectedly, we observed lower maternal IGF-II levels in smokers after the adjustment for other maternal characteristics. The mechanisms are unclear, and this finding requires confirmation in larger cohort studies.
β-cell function.
Smoking appears to affect glucose regulation and has been associated with increased insulin resistance and altered glucose homeostasis in adults [26]. The mechanisms responsible for smoking-induced glucose dysregulation are unclear. Animal studies have demonstrated that exposure to smoking or nicotine during the prenatal and neonatal periods could impair insulin sensitivity, decrease beta cell function, impair glucose tolerance, and increase the susceptibility to metabolic syndrome in the offspring [10–13]. A recent study suggests that nicotine may increase insulin resistance through selectively activate AMP-activated protein kinase α2 (AMPKα2) in adipocytes [27]. Little is known about the relationship between smoking during pregnancy and fetal β-cell function in humans. Our study is the first to explore this relationship in humans. We observed a borderline significantly higher cord proinsulin-to-insulin ratio (adjusted p = 0.06) in the newborns of smoking vs. non-smoking mothers independent of birth weight z score and gestational age, suggesting that tobacco may have an adverse impact on β-cell function in human fetuses/newborns. This finding is supported by data from an animal model study—prenatal nicotine exposure may decrease pancreatic islet size and number (which is a surrogate for β-cell function) in rats at the 7th postnatal day of life [11].
Leptin and adiponectin.
Adiponectin and leptin are important adipose tissue secreted hormones (adipokines) regulating energy balance and insulin sensitivity [28]. A few studies have explored the effect of maternal smoking on fetal leptin and adiponectin levels, but the findings have been inconsistent. Ozkan’s and Christos’s studies reported lower serum leptin levels comparing the neonates of smoking vs. nonsmoking mothers in cord blood or venous blood at the 7th postnatal day of life [29, 30]. In contrast, Helland’s and Chelchowska’s studies found no difference in cord blood leptin concentrations between the neonates of smokers and non-smokers [31, 32]. The reasons behind these inconsistencies may be partly due to the difference in sample size, the timing (early, mid or late gestation?) and method (self-report? cotinine?) in defining the smoking status. To our knowledge, only one study analyzed the effect of maternal smoking on fetal adiponectin levels. In a study of 85 healthy women, Chelchowska and colleagues observed significantly lower cord serum total adiponectin concentrations in the newborns of smoking women [32]. However, this finding could not be confirmed in our study of 238 mother-baby pairs in healthy pregnancies free of major chronic illnesses.
Strengths and limitations
Strengths of the study are the prospective pregnancy cohort, timely collection and processing of blood specimens and high-quality biochemical assays (low intra- and inter-assay coefficients of variation). The main limitation is the relatively small number of women in the smoking group. The study was powered to detect relatively large differences, but not powered to detect moderate differences in fetal metabolic health biomarkers. Another limitation is that smoking status was based on self-reports at mid and late gestation. We could not identify women who were previous smokers and quitted smoking in early pregnancy or before pregnancy. Such quitters might be common for woman smokers in planning pregnancy or on knowledge of pregnancy. This might explain the low prevalence of current smokers (at mid and late gestation) in the study cohort. These smoking quitters would have been mixed into the non-smoker group. However, such misclassifications would only tend to decrease the observed impact of smoking on cord blood metabolic health biomarkers. Smoking has commonly been assessed through self-report or a biomarker–mostly commonly, plasma or urine cotinine (a nicotine metabolite). Although plasma or urine cotinine measurements are more objective, they only reflect smoking exposure status in a relative short time period (about three days) [33]. Self-report is efficient and uniquely captures information about historical patterns of smoking [34]. In our study, maternal smoking status was identified through interviews twice during pregnancy, and virtually all (17/18) smokers reported smoking at both 24–28 and 32–35 weeks of gestation, indicating they were persistent smokers. Also, smoking may be prone to under-reporting due to social pressure [35]. However, such under-reporting misclassifications in smoking status would most likely tend to decrease the observed differences in cord blood biomarkers between the newborns of smokers vs. non-smokers.
Conclusions
Increasing evidence suggests that the intrauterine environment may affect fetal metabolic health [14,32,36]. Our study provides important preliminary evidence suggesting that maternal smoking may decrease fetal IGF-I levels, and may have an adverse impact on fetal β-cell function. The latter finding needs to be confirmed in larger cohort studies. We speculate that these changes in early life may be involved in the impact of maternal smoking on future risk of metabolic syndrome related disorders in the offspring.
Supporting Information
S1 Checklist. STROBE Statement—Checklist of items that should be included in reports of cohort studies.
https://doi.org/10.1371/journal.pone.0143660.s001
(DOC)
S2 Checklist. PLOS ONE Clinical Studies Checklist.
https://doi.org/10.1371/journal.pone.0143660.s002
(DOCX)
Acknowledgments
We would like to acknowledge the excellent professional work of research staff at Sainte-Justine Hospital Research Center in patient recruitment, follow-ups, data management and biochemical assays.
Author Contributions
Conceived and designed the experiments: FF ZCL ED. Performed the experiments: FF ZCL AD ED JZ. Analyzed the data: FF ZCL. Contributed reagents/materials/analysis tools: FF ZCL AD ED JZ. Wrote the paper: FF ZCL AD ED JZ.
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