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Correction: Molecular Cloning, Heterologous Expression, and Functional Characterization of an NADPH-Cytochrome P450 Reductase Gene from Camptotheca acuminata, a Camptothecin-Producing Plant

  • Xixing Qu,
  • Xiang Pu,
  • Fei Chen,
  • Yun Yang,
  • Lixia Yang,
  • Guolin Zhang,
  • Yinggang Luo

The images for Figs 7 and 8 are incorrectly switched. The image that appears as Fig 7 should be Fig 8, and the image that appears as Fig 8 should be Fig 7. The figure captions appear in the correct order. Please see the corrected Fig 7 here.

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Fig 7. Amino acid residues alignment of CamCPR, caa_locus_6894, caa_locus_12198, and caa_locus_112450 using Clustal Omega multiple aligment tool.

The identical amino acid residues between CamCPR and caa_locus_6894 were highlighted in red, between CamCPR and caa_locus_12198 were in green, and between CamCPR and caa_locus_112450 were in blue.

https://doi.org/10.1371/journal.pone.0139647.g001

Please see the corrected Fig 8 here.

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Fig 8. Enzymatic digestion and chemical degradation of tCamCPR.

The predicted cleavage sites of tCamCPR (a) and the detected peptide fragments by SDS-PAGE analysis (b). T, thrombin; H, Hydroxylamine; M, protein ruler; Lane 1, tCamCPR; Lane 2, thrombin; Enzyme digestion products of tCamCPR with 1 U / 50 μL (Lane 3) and 2 U / 50 μL (Lane 4) of thrombin were used [37]; Lanes 5 and 6, chemical degradation products of tCamCPR by hydroxylamine [38].

https://doi.org/10.1371/journal.pone.0139647.g002

Reference

  1. 1. Qu X, Pu X, Chen F, Yang Y, Yang L, Zhang G, et al. (2015) Molecular Cloning, Heterologous Expression, and Functional Characterization of an NADPH-Cytochrome P450 Reductase Gene from Camptotheca acuminata, a Camptothecin-Producing Plant. PLoS ONE 10(8): e0135397. pmid:26252645