In the Materials and Methods section, under the subheading Western blotting, the second sentence incorrectly refers to 0.5M EDTA. The correct concentration is 10mM EDTA. The correct sentence is: All transgenic combinations were entrained at 25°C under LD, and then the heads of flies with the w;GMR-GAL4/CyO;UAS-hGBA genotype collected at 11.00 a.m. were homogenized in extraction buffer containing 20 mM HEPES (pH 7.5), 100 mM KCl, 5% glycerol, 100 mM Na3VO4, 10 mM EDTA, 0.1% Triton-X, 10 mg/mL antipain, 10 mg/mL pepstatin-A, 10 mg/mL leupeptin, 24 TIU/mL aprotinin and 0.1 M phenylmethyl-sulfonyl-fluoride (PMSF).
Reference
Citation: Suzuki T, Shimoda M, Ito K, Hanai S, Aizawa H, Kato T, et al. (2015) Correction: Expression of Human Gaucher Disease Gene GBA Generates Neurodevelopmental Defects and ER Stress in Drosophila Eye. PLoS ONE 10(8): e0135619. https://doi.org/10.1371/journal.pone.0135619
Published: August 19, 2015
Copyright: © 2015 Suzuki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited