Figures
Abstract
The coastal mosquito Aedes togoi occurs more or less continuously from subarctic to subtropic zones along the coasts of the Japanese islands and the East Asian mainland. It occurs also in tropical Southeast Asia and the North American Pacific coast, and the populations there are thought to have been introduced from Japan by ship. To test this hypothesis, the genetic divergence among geographic populations of A. togoi was studied using one mitochondrial and three nuclear gene sequences. We detected 71 mitochondrial haplotypes forming four lineages, with high nucleotide diversity around temperate Japan and declining towards peripheral ranges. The major lineage (L1) comprised 57 haplotypes from temperate and subarctic zones in Japan and Southeast Asia including southern China and Taiwan. Two other lineages were found from subtropical islands (L3) and a subarctic area (L4) of Japan. The Canadian population showed one unique haplotype (L2) diverged from the other lineages. In the combined nuclear gene tree, individuals with mitochondrial L4 haplotypes diverged from those with the other mitochondrial haplotypes L1—L3; although individuals with L1—L3 haplotypes showed shallow divergences in the nuclear gene sequences, individuals from Southeast Asia and Canada each formed a monophyletic group. Overall, the genetic composition of the Southeast Asian populations was closely related to that of temperate Japanese populations, suggesting recent gene flow between these regions. The Canadian population might have originated from anthropogenic introduction from somewhere in Asia, but the possibility that it could have spread across the Beringian land bridge cannot be ruled out.
Citation: Sota T, Belton P, Tseng M, Yong HS, Mogi M (2015) Phylogeography of the Coastal Mosquito Aedes togoi across Climatic Zones: Testing an Anthropogenic Dispersal Hypothesis. PLoS ONE 10(6): e0131230. https://doi.org/10.1371/journal.pone.0131230
Editor: Igor Mokrousov, St. Petersburg Pasteur Institute, RUSSIAN FEDERATION
Received: April 8, 2015; Accepted: May 29, 2015; Published: June 24, 2015
Copyright: © 2015 Sota et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: Sequence data are deposited in the DNA Data Bank of Japan (DDBJ: accessions LC025655—LC025878). All other relevant data are within the paper and its Supporting Information files.
Funding: This study was partly supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Numbers 17405007, 23405009 and 15H02637 to TS. HY was supported by the Ministry of Higher Education High Impact Research (MoHE-HIR) grant H-50001-00-A000025.
Competing interests: The authors have declared that no competing interests exist.
Introduction
Major human disease vectors of the mosquito tribe Aedini (Culicidae: Culicinae) including the yellow fever mosquito Aedes aegypti and the Asian tiger mosquito A. albopictus are well known examples of anthropogenic dispersal across the world [1–4]. A recent example is the spread of Aedes japonicus to the United States, Canada and European countries where it was probably introduced in used tires from Japan [5–6]. Many species of Aedini breed in small water bodies including artificial containers, lay desiccation and freeze resistant eggs, and hence have potential to be transported by ships as eggs or larvae in water tanks or used tires. Finding the origin and means of dispersal of disease-vectors spreading across the world is crucial to predicting the risk of vector colonization and implementing appropriate control programs.
Anthropogenic dispersal may have resulted in the wide distribution across climatic zones in the aedine species Aedes togoi, which is widely distributed from subtropical to subarctic East Asia, breeding in coastal rock pools as well as artificial containers [7]. This species is a known vector of human and canine filariasis, and Japanese encephalitis virus has been isolated from field-collected mosquitoes [7–10]. The occurrence of A. togoi has also been reported from tropical Southeast Asia [11–13] and the Pacific coast of Canada and the USA [14–16]. These peripheral populations were discovered in the late 20th century and may have been introduced from Japan by ships. In the temperate zone, this species has several generations per year, overwinters as diapausing larvae or eggs and shows facultative autogeny (egg production without blood feeding) [17–18]. Over their geographic range, A. togoi populations differ in life history traits such as body size, larval diapause (delayed development in response to short-day condition) and autogeny [19–22]. The Canadian population has life-history traits similar to those of temperate Japanese populations [20–24], consistent with the introduction hypothesis. However, tropical Asian populations show traits different from temperate ones: small body size, obligatory autogeny and lack of larval diapause [19–22]. This finding raises doubts about the introduction hypothesis [19]. The genetic relationships among different populations might resolve the origins of the peripheral A. togoi populations. In this study, we therefore studied geographic genetic variations in A. togoi using mitochondrial and nuclear gene sequences to investigate the validity of the anthropogenic introduction hypothesis.
Materials and Methods
Sampling and DNA extraction
This study did not include protected or endangered species, and no permission was required to collect the study species, Aedes togoi and Aedes savoryi. All the collection sites were in unprotected areas accessible to the public, and permission from landowners was obtained whenever necessary. A total of 282 larvae or adults of A. togoi were collected at 42 field sites (S1 Table; Fig 1). Larval and adult specimens were fixed in 95–99% ethanol and stored in a refrigerator until DNA was extracted. Total genomic DNA was extracted from individual larvae or adults using a Wizard DNA purification kit (Promega). We also used adult specimens (preserved in ethanol) from 3 old laboratory colonies originating in Taiwan, Malaysia and Thailand (S1 Table). We used 8 larvae of A. savoryi from Ogasawara (Bonin) Islands, Japan, as the outgroup (S1 Table). Aedes savoryi is morphologically similar to A. togoi and considered as the sister species [25]. We examined the sister relationship based on three nuclear gene sequences from 12 culicine species including A. togoi and A. savoryi (see S2 Table and S1 Fig for methods).
(A) Sample localities in Asia and Canada. Sample size (n) and the number of haplotypes (h) for each locality are indicated. (B) Localities in Japan, with one locality in Taiwan.
DNA sequencing
We used partial sequences of the mitochondrial cytochrome oxidase subunit I (COI) gene and nuclear 28S rRNA (28S), CAD and white genes for phylogenetic analysis. CAD and white genes were selected based on a previously published mosquito molecular phylogeny [26]. A partial COI gene region was amplified using primers C1-J-2195: 5’-TTG ATT TTT TGG TCA TCC AGA AGT-3’ and TL2-N-3014: 5’-TCC AAT GCA CTA ATC TGC CAT ATT A-3’ [27]. Partial sequences of 28S rRNA, white, and CAD genes were amplified and sequenced using the following primers. For 28S, Dipt28SF: 5’-AGG GGA GGA AAA GAA ACT AAC AAG GA-3’ and Dipt28SR1: 5’-CTT GGT CCG TGT TTC AAG ACG GGT C-3’ [28]. For CAD, 819 bp exon sequence, CAD F1: 5’-GAA AGA CCC GGT GGA GTG CTG CTC ACC-3’; CAD R1: 5’-GCC ATC ACT TCA CCG ACA CTY TTC AT-3’. For white (825 bp aligned sequence encompassing 3 exon and two intron) white F1: 5’-TAY AAT CCG GCG GAY TTC TAC GTC CAA ATG-3’; white R1: 5’-ATC AGG AAY GGA ATA ATC ACC GGT GGR CCG-3’, and internal primers only for sequencing, white F2: 5’-CAA ACG GCG GTA AGA ATC AG-3’ and white R2: 5’-TGG AAG CTC AGC AGA AAA CA-3’. We redesigned the CAD and white primers with reference to the published sequences of these genes for some aedine species [26]. Sequence data are deposited in the DNA Data Bank of Japan (DDBJ: accessions LC025655—LC025878).
Phylogenetic, population genetic and divergence time analysis
Alignment of sequences was done with MUSCLE version 3.831 [29]. Maximum-likelihood (ML) analysis was done with RAxML v8.0.0 [30] with rapid bootstrapping procedure using 1000 bootstrap analyses. We used partitioned analysis discriminating three codon positions, intron and rRNA sequence, and applied GTR+G substitution model to each partition because it is recommended as the most versatile model by the author of RAxML [30]. For COI and CAD gene sequences and the concatenated exon sequence of white gene, three codon positions were treated as different partitions. Each of the entire 28S gene sequence and the concatenated intron sequence of white gene was treated as one partition. A combined ML analysis was conducted for the nuclear gene sequences. We also constructed a phylogenetic network for the COI gene sequence to demonstrate a star-like topology of haplotype sequences using the neighbor-net algorithm in SplitsTree4 v4.13.1 [31]. Node credibility was assessed using 1000 bootstrap resampling. In addition, we constructed a population tree based on mean uncorrected p-distance between populations obtained using MEGA version 5.2.2 [32] and using the neighbor-joining method in PHYLIP version 3.69 [33]. To show latitudinal trend in genetic diversity, the nucleotide diversity of COI gene sequences at each locality was calculated using Arlequin version 3.5.1.3 [34].
The divergence times between COI haplotype lineages were estimated using a Bayesian relaxed clock model analysis implemented in BEAST version 1.80 [35]. For time calibration, we used disconnection of the Ryukyu Islands from the main islands of Japan (Kyushu, Shikoku and Honshu) 1.7 million years ago (mya). The Ryukyu Islands were separated from the East Asian mainland by tectonic movement and enlargement of the Okinawa trough during the late Pliocene and the early Pleistocene, and the land connection between the Ryukyu Islands and the main islands of Japan disappeared by 1.7 mya [36]. We assumed this geological event was related to divergence of the haplotype lineage of A. togoi specific to Ryukyu and set the node age prior to the branching of the Ryukyu haplotype lineage as a normal distribution with a mean of 1.7 my and a standard deviation of 0.17 my. In BEAST analysis, the COI sequences were partitioned by three codon positions and a GTR+G substitution model was used for each partition. We used an uncorrelated relaxed clock model with lognormal distribution of branch-specific rates, and the Markov Chain Monte Carlo (MCMC) run was performed for 108 generations with a sampling frequency of 104. The results were checked for effective sample sizes using Tracer version 1.6 [37]; and a consensus tree was obtained using TreeAnnotator version 1.8.0 in BEAST discarding the initial 1000-generation data as burn-in. Note that the calibration method we employed is uncertain for the relationship between the geological event and the haplotype divergence. However, we have also tested a divergence time estimate using a time-dependent evolutionary rate of insect mitochondrial gene sequences [38–39] and obtained divergence times that are almost within the 95% HPD (highest probability density) intervals of those obtained by the above analysis (see S3 Table).
Results
We confirmed the appropriateness of A. savoryi as the outgroup using three nuclear gene sequences (S2 Table; S1 Fig). We obtained an 817-bp COI gene sequence for 282 A. togoi and 8 A. savoryi specimens. We detected a total of 71 haplotypes for the mitochondrial COI sequence of A. togoi (Fig 2; S4 Table; DDBJ accessions LC025655—LC025758). The haplotypes were divided into four lineages L1—L4 (Fig 2), which had an estimated coalescent time of 2.7 mya (95% HPD interval, 1.8–4.2 mya; i.e. late Pliocene to early Pleistocene; see also S2 Fig). Of these lineages, L1 haplotypes were found from subarctic Hokkaido to subtropical Amami in Japan, highly diversified (57 haplotypes) and further clustered into four subgroups L1a—L1d, that comprised 20, 4, 14, and 19 haplotypes, respectively. These subgroups occurred in different regions; L1d occurred in the northern coast of the Sea of Japan, L1b and L1c in the middle latitudes, and L1a in the southwest region (Fig 1; S4 Table). L1a—L1c haplotypes occurred also in Taiwan, and L1c in the coasts of China and Peninsular Malaysia (Fig 1). In Peninsular Malaysia, only one haplotype of L1c (H65; S4 Table) was detected, which was also found in Japan. Three laboratory colonies from Peninsular Malaysia, Thailand and Taiwan (not included in Fig 1) showed the same single L1c haplotype (H54), which was common in temperate Japan (S4 Table). Lineage L2 was represented by the only haplotype from Canadian populations, and it was found nowhere else (Fig 2). L2 was estimated to have diverged from L1 approximately 1 mya (95% HPD interval, 0.6–1.5 mya). L3 (7 haplotypes) was unique to the Ryukyu Islands from Amami to Yonaguni. Mosquitoes in the northernmost Ryukyu island, Amami also had L1a haplotypes, which were dominant in southern temperate Japan. Finally, a lineage L4 was found in east Hokkaido (6 haplotypes). The relationships between the geographic populations are depicted by the population tree based on mean sequence difference of COI sequences (Fig 3A); this tree shows that the populations from southern China, Taiwan and Peninsular Malaysia are closely related to populations in temperate Japan. The nucleotide diversity of COI sequences at each locality was high at middle latitude around 35°N and at a site on Amami Island (28.48°N) harboring both L1 and L3 haplotypes (Fig 3B).
H-numbers (H001—H071) are haplotypes of A. togoi, and O001—O003 are those of A. savoryi (outgroup). In the ML tree, numerals on branches are bootstrap percentages (shown when >50%); arrows indicate haplotypes from Taiwan, southern China and Peninsular Malaysia, which occurred also in Japan except H34, H46 and H51; for nodes with closed circles, median node ages estimated by BEAST analysis are indicated with 95% HPD intervals in parentheses (see S3 Fig for details). In the network, numerals on branches are bootstrap percentages (shown when >50%).
(B) Latitudinal change in the nucleotide diversity of COI sequences at each locality. Bars show ± SD. Open circles, Japanese islands except Ryukyu Islands; gray circles, Ryukyu Islands; black circles, outside Japan.
We obtained 959-bp 28S, 819-bp CAD and 825-bp white gene sequences for 49 specimens of A. togoi (DDBJ accessions LC025729—LC025878). In the combined phylogenetic analysis of these nuclear gene sequences (Fig 4; see S3 Fig for individual gene trees), individuals with the same mitochondrial lineages tended to clump with one another although those with L1 haplotypes were polyphyletic. As in the mitochondrial gene tree, individuals with L4 mitochondrial haplotypes from eastern Hokkaido were distinct from all individuals with the other mitochondrial haplotypes in the nuclear gene trees. Canadian individuals showed unique substitutions in 28S and white genes, forming a monophyletic clade. Further, individuals from southern China, Taiwan and Peninsular Malaysia formed a strongly supported clade due to unique substitutions in CAD and white gene sequences. Substitutions in coding sequences of CAD and white genes were all synonymous.
Numerals on branches are bootstrap percentages (shown when >50%). Mitochondrial lineage of each individual is indicated.
Discussion
The mitochondrial COI haplotypes of A. togoi consisted of four distinct lineages, which were estimated to have diverged since the late Pliocene or the early Pleistocene; the haplotype diversity and nucleotide diversity were highest in the temperate zone in Japan. The alternation of haplotype lineages across geographic and climatic regions in Japan, together with divergence in nuclear gene sequences corresponding to that in mitochondrial haplotypes, could be the effect of isolation by distance, but geographic disjunctions of haplotype lineages between temperate and subtropical or subarctic zones are consistent with the existence of geographic barriers, which may be associated with local adaptation.
Notably, the populations of the subtropical Ryukyu Islands of Japan had a haplotype lineage different from those in temperate Japan, suggesting the absence of continuous gene flow between these regions. Also notably, the population in Taiwan did not have the Ryukyu haplotypes despite their geographic proximity. In southern China, Taiwan and Peninsular Malaysia, A. togoi populations had only haplotypes of the L1 lineage, the same haplotypes as those found in temperate Japanese populations. These results suggest that the Southeast Asian populations have not been isolated long from those in temperate Japan. However, based on the nuclear gene sequences, the Southeast Asian populations formed a monophyletic group diverging from temperate Japanese populations. Therefore, the present Southeast Asian populations may have a common origin, either by natural or anthropogenic dispersal; a founder population might have subsequently dispersed while being adapted to subtropical and tropical environments. The dispersal of A. togoi might have been facilitated by transportation by ships among Asian countries, although direct evidence is lacking. During the mid-20th century, Aedes aegypti was introduced to Japan from Southeast Asia probably by ship; at this time A. togoi was common around the ports in Japan [40]. Also, Ramalingam [13] considered ships carrying cargo such as iron ore and timber between the east coast of Peninsular Malaysia and Japan as candidate transporters.
Although our genetic data suggest that the Southeast Asian populations of A. togoi have not been isolated very long from the temperate Japanese populations, the tropical populations from Thailand and Peninsular Malaysia had different life-history characteristics from those of temperate populations in the laboratory such as the lack of larval diapause, obligatory autogeny and small body size [19,21]. Another tropical population from Hainan I., China, also showed obligatory autogeny [41]. These differences may be evidence that the tropical populations are indigenous [19]. However, life history traits such as diapause and body size can evolve rapidly in dipteran insects including aedine mosquitoes [42–45]. Therefore, the life-history characteristics of A. togoi in the tropics may have evolved during their recent range expansion in East and Southeast Asia.
The origin of Canadian populations is enigmatic, as there is a gap of more than 5000-km in their distribution between East Asia and the North American Pacific coast. The fact that the Canadian population has life-history characteristics similar to temperate populations in Japan [19–20] does not contradict either of the hypotheses: anthropogenic introduction or natural distribution, because both climates are temperate. We found only a single, unique COI haplotype from four locations in British Columbia separated up to 150 km. One explanation may be that the Canadian population is an introduced one from somewhere in Asia, which started from few individuals or has experienced a strong bottleneck. Transportation by ship has been possible since the late 19th century, as there was already trade between Yokohama, Japan and Vancouver then. Mogi [46] noted that the underground mosquito Culex pipiens f. molestus might have been transported from North America to Japan by ship by the mid-20th century. However, we cannot exclude the possibility that A. togoi could have spread naturally across Beringia unless the origin of the unique haplotype is resolved by more extensive sampling in East Asia. In this hypothesis, A. togoi might have undergone stepping stone dispersal along the Kuril Islands, Kamchatka, the Aleutian Islands and Alaska to British Columbia. The estimated divergence time of the Canadian haplotype from L1 haplotypes in Asia was 1 mya (credible range, 0.6–1.5 mya), spanning several glacial cycles during which such dispersal across Beringia might have been possible. The last land bridge between Siberia and Alaska was not severed until just before 13,000 ya [47]. On the northeast Pacific coast, the easternmost occurrence of A. togoi is reported from South Kuril [7], close (<50 km) to eastern Hokkaido where the most diverged L4 haplotypes occurred. Aedes togoi may occur further east, on the North Kurils, Kamchatka, or the Aleutians; a survey of A. togoi in these regions is needed to understand the dispersal pattern of A. togoi in the subarctic zone and obtain a clue to the origin of the North American population.
Lastly, we note that, even though A. togoi can disperse over long distances by themselves or by human transportation, the survival of colonizers may be limited in exotic habitats, and their populations may often be subject to the loss of genetic diversity or extinction. For example, A. togoi once colonized the subtropical Ogasawara Islands in the Pacific Ocean 1000-km south of Tokyo in 1960s [48], probably as a result of transportation by ships. However, A. togoi now appears to be extinct, as only the native sister species A. savoryi was abundant in rock pools in later surveys (e.g., [49]; 2010 by K. Kawakami, personal communication). On the Ogasawara islands, reproductive interference with the sister species may make the survival of invasive species difficult [50–52]. Irrespective of the presence of closely related species, survival of introduced mosquito populations is limited by abiotic and biotic factors in exotic habitats [53]. Scarcity of large, stable rock pools on the seacoast would greatly limit the population persistence of A. togoi. On the eastern coast of Peninsular Malaysia, suitable larval habitats for A. togoi (rock pools or holes) are scarce, and the A. togoi population there appeared to be very small according to our field survey (in 1989 and 2010 by TS and HSY).
Our study revealed that the genetic diversity of A. togoi centers on the Japanese Islands, and the genetic composition varies among geographic and climatic regions, consistent with the existence of geographic barriers related to climatic adaptation as well as the effect of isolation-by-distance. The genetic composition of southern China, Taiwan and Southeast Asian populations is similar to those in temperate Japan, consistent with gene flow between these regions, whereas the Canadian population has a genetic composition distinct from that of Asia. Our study has not provided strong support for recent anthropogenic introduction of A. togoi from temperate Japan to tropical Southeast Asia or Canada, although transportation by ship may have promoted gene flow between Japan and other Asian countries. More extensive geographic sampling of A. togoi populations along the coast of East Asian mainland and around the Pacific rim will be needed to resolve the role of natural and anthropogenic dispersal in forming the present distribution of this species.
Supporting Information
S1 Fig. Phylogenetic relationships among culicine species showing the sister relationship between Aedes togoi and A. savoryi.
The phylogenetic position of A. togoi and its sister relationship to A. savoryi based on a morphological analysis were examined using three nuclear gene sequences (CAD, enolase, white) newly obtained for A. togoi and A. savoryi with previously published data for 10 species from aedine and non-aedine Culicinae (S2 Table). The maximum-likelihood tree obtained by RAxML (partitioned by each codon position in each gene; GTR+gamma model; 1000 bootstrap analysis) weakly supported the monophyly of Aedini but strongly supported the sister relationship between A. togoi and A. savoryi within the strongly supported group of some aedine species.
https://doi.org/10.1371/journal.pone.0131230.s001
(PDF)
S2 Fig. Divergence time estimation by Bayesian relaxed clock analysis with BEAST.
Median nodes age and 95% highest probability density intervals are indicated for major nodes with black circles. On the branches shown are posterior probabilities and bootstrap percentages in the maximum-likelihood analysis (shown when >0.5 or >50%).
https://doi.org/10.1371/journal.pone.0131230.s002
(PDF)
S3 Fig. Maximum-likelihood trees for three nuclear genes.
Bootstrap percentages are shown on the branches when >50%.
https://doi.org/10.1371/journal.pone.0131230.s003
(PDF)
S1 Table. List of samples for DNA analysis with information of haplotype groups.
https://doi.org/10.1371/journal.pone.0131230.s004
(PDF)
S2 Table. GenBank/DDBJ accession numbers for the three genes (exon only) used in the phylogenetic analysis of culicine mosquitoes.
https://doi.org/10.1371/journal.pone.0131230.s005
(PDF)
S3 Table. Mean sequence divergence (uncorrected p) and estimated divergence times between mitochondrial COI haplotype lineages.
https://doi.org/10.1371/journal.pone.0131230.s006
(PDF)
S4 Table. Distribution of mitochondrial COI haplotypes.
https://doi.org/10.1371/journal.pone.0131230.s007
(PDF)
Acknowledgments
We thank M. Sato, H.B. Liang, Y. Okuzaki, K. Tsuji, N. Nagata, T. Yashiro, M. Toda, W. Choochote, A. Mori, K. Kawakami, H. Sugawara, J. Konuma, M. Hoso, R. G. Bennett and J. Gower Taylor for sampling and specimens.
Author Contributions
Conceived and designed the experiments: TS MM. Performed the experiments: TS. Analyzed the data: TS. Contributed reagents/materials/analysis tools: TS PB MT HY MM. Wrote the paper: TS PB MT HY MM.
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