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Open Access

Correction

Correction: Quantitative Assessment of Antibody Internalization with Novel Monoclonal Antibodies against Alexa Fluorophores

  • The PLOS ONE Staff

There is an error in the Correction published on May 15, 2015. The corrected headings for Fig 1B are “Cells stained with 1C1-A488” and “Cells stained with 1C1-A594”, not “Beads stained with 1C1-A488” and “Beads stained with 1C1-A594”. The publisher apologizes for the error. The correct text is:

There are a number of errors in the headings for Fig 1B, “Quenching by anti-Alexa Fluor mAbs.” “Beads coated with 1C1-A488” and “Cells stained with 1C1-A488” should be “Beads coated with 1C1-A594” and “Cells stained with 1C1-A594”. Please see the corrected Fig 1 here.

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Fig 1. Quenching by anti-Alexa Fluor mAbs.

(A) Fluorescence of Alexa Fluor 488 (A488) on microbeads coated with 1C1-A488 or PC-3 cells stained with 1C1-A488 was quenched with a titration of the benchmark, a rabbit anti-A488 polyclonal, or 1 of 3 anti-A488 mAbs. One representative experiment of multiple is shown. (B) Fluorescence of Alexa Fluor 594 (A594) on microbeads coated with 1C1-A594 or PC-3 cells stained with 1C1-A594 was quenched with a titration of 1 of 3 anti-A594 mAbs. One representative experiment of multiple is shown. (A, B) Median fluorescence intensities (MFIs) at each anti-A488 or anti-A594 mAb concentration were normalized against a buffer control. The chimeric IgG1 isotype control was used as a non-quenching mAb control. The IC50 values (microgram/ml) of quenching and the corresponding 95% confidence intervals (95% CI) are listed for both the microbead- and cell-based titrations.

https://doi.org/10.1371/journal.pone.0130106.g001

References

  1. 1. Liao-Chan S, Daine-Matsuoka B, Heald N, Wong T, Lin T, Cai AG, et al. (2015) Quantitative Assessment of Antibody Internalization with Novel Monoclonal Antibodies against Alexa Fluorophores. PLoS ONE 10(4): e0124708. pmid:25894652
  2. 2. The PLOS ONE Staff (2015) Correction: Quantitative Assessment of Antibody Internalization with Novel Monoclonal Antibodies against Alexa Fluorophores. PLoS ONE 10(5): e0128729. pmid:25978518

Citation: The PLOS ONE Staff (2015) Correction: Quantitative Assessment of Antibody Internalization with Novel Monoclonal Antibodies against Alexa Fluorophores. PLoS ONE 10(6): e0130106. https://doi.org/10.1371/journal.pone.0130106

Published: June 12, 2015

Copyright: © 2015 The PLOS ONE Staff. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited