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Correction: CDK-Dependent Nuclear Localization of B-Cyclin Clb1 Promotes FEAR Activation during Meiosis I in Budding Yeast

  • The PLOS ONE Staff

Figure 6 is incorrect. Panels 6C and 6E in Figure 6 are duplicated. Panel 6E is incorrectly displayed as panel 6C. The authors apologize for this error and have provided a revised Figure 6 with the correct panel 6C, which can be viewed here. The raw blots for Figure 6 are also available with this correction.

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Figure 6. Phosphorylation of Clb1 during meiosis requires its localization to the nucleus.

A) Cultures of CLB1-ha PDS1-myc18, CLB1-NES-ha PDS1-myc18 and CLB1-NLS-ha PDS1-myc18 cells were induced to enter meiosis by transferring them to SPM. Nuclear division in the three cultures was determined by DAPI staining and the data are represented graphically. B) Cultures of CLB1-ha PDS1-myc18 and CLB1-NES-ha PDS1-myc18 cells were induced to enter meiosis by transferring them to SPM. Whole cell extracts were analysed by Western blotting for Clb1 phosphorylation. C) Cultures of CLB1-ha PDS1-myc18 and CLB1-NLS-ha PDS1-myc18 cells were induced to enter meiosis by transferring them to SPM. Whole cell extracts were analysed by Western blotting for Clb1 phosphorylation. D) Cultures of PCLB2CDC20 CLB1-ha and PCLB2CDC20 CLB1-NES-ha cells were induced to enter meiosis by transferring them to SPM. Samples were taken at time 0 and hourly from 4–10 hours for whole cell extracts and analysed by Western blotting. E) Cultures of PCLB2CDC20 CLB1-ha and PCLB2CDC20 CLB1-NLS-ha cells were induced to enter meiosis by transfer to SPM. Samples were taken at time 0 and hourly from 4–10 hours for whole cell extracts and analysed by Western blotting.

https://doi.org/10.1371/journal.pone.0079001.g006

Reference

  1. 1. Tibbles KL, Sarkar S, Novak B, Arumugam P (2013) CDK-Dependent Nuclear Localization of B-Cyclin Clb1 Promotes FEAR Activation during Meiosis I in Budding Yeast. PLoS ONE 8(11): e79001