-->PONE-D-25-64821-->-->Acaricidal Activity of Plant Extracts Against Amblyomma variegatum Tick Species in Waghimra, Northeastern Ethiopia-->-->PLOS One

Dear Dr. tibebu,

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We look forward to receiving your revised manuscript.

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Kandasamy Ulaganathan

Academic Editor

PLOS One

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3. Please provide additional details regarding consent obtained from the owners of the animals and mention the detailed procedure.

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“The authors would like to express their sincere gratitude to the animal owners for their cooperation. We also wish to acknowledge the community animal health workers in the study area for their invaluable assistance in facilitating the fieldwork. Finally, we extend our thanks to the Amhara Region Agricultural Research Institute (ARARI) for the financial and logistical support.”

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Reviewers' comments:

Reviewer's Responses to Questions

-->Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. -->

Reviewer #1: No

Reviewer #2: Yes

Reviewer #3: Yes

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-->2. Has the statistical analysis been performed appropriately and rigorously? -->

Reviewer #1: No

Reviewer #2: Yes

Reviewer #3: Yes

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-->3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.-->

Reviewer #1: No

Reviewer #2: Yes

Reviewer #3: Yes

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-->4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.-->

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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-->5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)-->

Reviewer #1: Dear auturs:

The manuscript addresses a topic of interest; however, it does not yet meet the standards required for publication.

Technical soundness: The study lacks sufficient methodological rigor. Key details regarding experimental design, controls, replication, and sample size justification are insufficiently described, which limits confidence in the validity of the findings.

Statistical analysis: The statistical analyses are not rigorous enough to support the conclusions. Analyses are largely descriptive, with limited justification of statistical tests and absence of effect sizes or confidence intervals.

Data–conclusion alignment: Several conclusions appear overstated and are not fully supported by the presented data. Conclusions should be more cautious and strictly aligned with the results.

Presentation and clarity: Improvements are needed in data presentation (figures/tables) and overall clarity to allow readers to better interpret the findings.

Substantial revision, including strengthening the methodology, improving statistical rigor, and revising conclusions, would be required before the manuscript could be reconsidered.

Reviewer #2: The current may consider for publication. Check the references list and text reference should be matched and proper format of the journal.. The above manuscript have good information about the tick control through natural products.

Reviewer #3: I thank the authors for their efforts in preparing this manuscript, which addresses a relevant topic in parasite control using plant-based, environmentally friendly alternatives, but the study in its current form suffers from methodological, statistical, and editorial weaknesses that require substantial revision before acceptance for publication.

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-->6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

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Reviewer #1: No

Reviewer #2: Yes: Dr Sachin Kumar (Ph.D, NPDF)

Reviewer #3: Yes: Mohamed Mahmoud Baz

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Attachments

Attachment

Submitted filename: Review Report_PONE-D-25-64821.docx

Acaricidal Activity of Plant Extracts against Amblyomma variegatum in Waghimra, Northeastern Ethiopia

PONE-D-25-64821R1

Dear Dr. tibebu,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Kandasamy Ulaganathan

Academic Editor

PLOS One

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

-->Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.-->

Reviewer #2: All comments have been addressed

Reviewer #3: All comments have been addressed

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-->2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. -->

Reviewer #2: Yes

Reviewer #3: Yes

**********

-->3. Has the statistical analysis been performed appropriately and rigorously? -->

Reviewer #2: Yes

Reviewer #3: Yes

**********

-->4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.-->

Reviewer #2: Yes

Reviewer #3: (No Response)

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-->5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.-->

Reviewer #2: Yes

Reviewer #3: Yes

**********

-->6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)-->

Reviewer #2: After revision the current manuscript is considerably for publication.. The research paper is good for all the researchers who working on tick research.

Thank you

Reviewer #3: Dear Authors,

I commend the authors for their excellent work and appreciate their effort addressing the reviewers' comments, resulting in a manuscript now ready for publication. I would only ask the authors to conduct a final review of the manuscript before it goes to print to correct some linguistic errors. This does not negate the manuscript's readiness and acceptance for publication.

- Please abbreviate the names of plant and animal species after mentioning them for the first time.

- After accepting the track change corrections, please adjust the spacing and note that some text is written in bold.

The manuscript is adequate and can be accepted as is, and it doesn't need it back

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-->7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.-->

Reviewer #2: Yes: Dr Sachin Kumar

Reviewer #3: Yes: Mohamed Mahmoud Baz

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