Peer Review History

Original SubmissionJune 11, 2024
Decision Letter - Akhtar Malik Muhammad, Editor

PONE-D-24-22328Examination of the spatial-temporal variations in terrestrial water reserves and the efficiency of green water usage in China's three northeastern provincesPLOS ONE

Dear Dr. yanying,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

==============================

Dear Authors,

Thank you for submitting the revised manuscript " Examination of the spatial-temporal variations in terrestrial water reserves and the efficiency of green water usage in China's three northeastern provinces" to PLOS ONE.

Reviewers 3 and 4 have not recommended your paper and suggest major/minor revisions. The reviewer’s comments need to be addressed to improve the paper quality. I go through the revised manuscript and reviewers’ comments. I suggest you should address the reviewer’s comments and resubmit a revised version.

Yours sincerely

Dr. Malik Muhammad Akhtar

Academic Editor

PLOS ONE

==============================

Please submit your revised manuscript by Dec 16 2024 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.
  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.
  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols. Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols.

We look forward to receiving your revised manuscript.

Kind regards,

Akhtar Malik Muhammad, PhD, Postdoc

Academic Editor

PLOS ONE

Journal requirements:

When submitting your revision, we need you to address these additional requirements.

1. Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The PLOS ONE style templates can be found at

https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and

https://journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf

2. In your Methods section, please include additional information about your dataset and ensure that you have included a statement specifying whether the collection and analysis method complied with the terms and conditions for the source of the data.

3. Please amend either the title on the online submission form (via Edit Submission) or the title in the manuscript so that they are identical.

4. We note that Figures 1 and 2 in your submission contain [map/satellite] images which may be copyrighted. All PLOS content is published under the Creative Commons Attribution License (CC BY 4.0), which means that the manuscript, images, and Supporting Information files will be freely available online, and any third party is permitted to access, download, copy, distribute, and use these materials in any way, even commercially, with proper attribution. For these reasons, we cannot publish previously copyrighted maps or satellite images created using proprietary data, such as Google software (Google Maps, Street View, and Earth). For more information, see our copyright guidelines: http://journals.plos.org/plosone/s/licenses-and-copyright.

We require you to either (1) present written permission from the copyright holder to publish these figures specifically under the CC BY 4.0 license, or (2) remove the figures from your submission:

1. You may seek permission from the original copyright holder of Figures 1 and 2 to publish the content specifically under the CC BY 4.0 license. 

We recommend that you contact the original copyright holder with the Content Permission Form (http://journals.plos.org/plosone/s/file?id=7c09/content-permission-form.pdf) and the following text:

“I request permission for the open-access journal PLOS ONE to publish XXX under the Creative Commons Attribution License (CCAL) CC BY 4.0 (http://creativecommons.org/licenses/by/4.0/). Please be aware that this license allows unrestricted use and distribution, even commercially, by third parties. Please reply and provide explicit written permission to publish XXX under a CC BY license and complete the attached form.”

Please upload the completed Content Permission Form or other proof of granted permissions as an ""Other"" file with your submission

In the figure caption of the copyrighted figure, please include the following text: “Reprinted from [ref] under a CC BY license, with permission from [name of publisher], original copyright [original copyright year].”

2. If you are unable to obtain permission from the original copyright holder to publish these figures under the CC BY 4.0 license or if the copyright holder’s requirements are incompatible with the CC BY 4.0 license, please either i) remove the figure or ii) supply a replacement figure that complies with the CC BY 4.0 license. Please check copyright information on all replacement figures and update the figure caption with source information. If applicable, please specify in the figure caption text when a figure is similar but not identical to the original image and is therefore for illustrative purposes only.

The following resources for replacing copyrighted map figures may be helpful:

USGS National Map Viewer (public domain): http://viewer.nationalmap.gov/viewer/

The Gateway to Astronaut Photography of Earth (public domain): http://eol.jsc.nasa.gov/sseop/clickmap/

Maps at the CIA (public domain): https://www.cia.gov/library/publications/the-world-factbook/index.html and https://www.cia.gov/library/publications/cia-maps-publications/index.html

NASA Earth Observatory (public domain): http://earthobservatory.nasa.gov/

Landsat: http://landsat.visibleearth.nasa.gov/

USGS EROS (Earth Resources Observatory and Science (EROS) Center) (public domain): http://eros.usgs.gov/#

Natural Earth (public domain): http://www.naturalearthdata.com/

Additional Editor Comments (if provided):

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Partly

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: No

**********

3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: This articel is designed to evaluate the spatial-temporal variations in terrestrial water reserves and the efficiency of green water usage in China's three northeastern provinces. The overall methodology and procedures were valid. However, the ABSTRACT should be re-summarized as there are no summarized conclusions and proper implications.

Reviewer #2: This paper uses GRACE data and statistical data to measure and evaluate the water resource utilization efficiency of 34 prefecture-level cities in the three northeastern provinces from 2003 to 2020 There are a few suggestions for further revision:

1.The entire paper should use a two end aligned format.

2.The title level is chaotic, for example, 1, 2, 3, etc. appear below 2.2.1..

3.The introduction section should elaborate on the importance and significance of this research in detail.

4.The first column of Table 2, Table 4 have two Year headers that need to be modified.

5.The clarity of most figures is low and needs to be improved.

6.The paper mentions the use of Tobit model to analyze the influencing factors of water resource utilization efficiency, but the application and analysis of formulas (7) and (8) in Tobit model are not used in the paper.

7.In the analysis of innovative points in the research content, there is no explanation of the innovative content, but rather a more accurate description of the research content is needed.

8.What is the basis for determining the evaluation index system?

9.I highly recommend to write limitations of the study and recommendation for future study at the end of conclusion.

10.The discussion on policies is not in-depth enough. It is recommended to include more policy analysis.

**********

6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

**********

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.

Revision 1

Dear Reviewer and Editor:

We sincerely appreciate the constructive comments provided by the reviewers and editors regarding our manuscript. Your insights and guidance have served as a ladder for our continuous improvement, significantly enhancing the quality of our work. We deeply regret the issues identified in the manuscript and assure you that we will internalize the advice from experts in our future research and studies, striving for higher levels of scholarly achievement. Below is a detailed response addressing each comment. The reviewers' comments are referenced using italicized font and numbering, while our responses are presented in regular font. Modifications and additions are highlighted in red for emphasis.

Recommendation 1: Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming.

Response 1:

Thank you very much for your suggestion. I have made the modifications according to PLOS ONE's style requirements

Recommendation 2: In your Methods section, please include additional information about your dataset and ensure that you have included a statement specifying whether the collection and analysis method complied with the terms and conditions for the source of the data.

Response 2:

Thank you very much for your suggestion. The data is publicly available. And the data collection and analysis method complied with the terms and conditions set by the University of Texas for the use of these data

Recommendation 3: Please amend either the title on the online submission form (via Edit Submission) or the title in the manuscript so that they are identical.

Response 3:

Thank you very much for your suggestion. The modifications have been made as required.

Recommendation 4: We note that Figures 1 and 2 in your submission contain [map/satellite] images which may be copyrighted. All PLOS content is published under the Creative Commons Attribution License (CC BY 4.0), which means that the manuscript, images, and Supporting Information files will be freely available online, and any third party is permitted to access, download, copy, distribute, and use these materials in any way, even commercially, with proper attribution. For these reasons, we cannot publish previously copyrighted maps or satellite images created using proprietary data, such as Google software (Google Maps, Street View, and Earth).

Response 4:

Thank you very much for your suggestion. I have replaced the image with copyright issues.

Recommendation 5: This articel is designed to evaluate the spatial-temporal variations in terrestrial water reserves and the efficiency of green water usage in China's three northeastern provinces. The overall methodology and procedures were valid. However, the ABSTRACT should be re-summarized as there are no summarized conclusions and proper implications.

Response 5:

Thank you very much for your suggestions and advice. It is your help that enables me to continually improve. It is your guidance that has enabled us to continually improve. I have rewritten the abstract and summarized the conclusion again.

Recommendation 6: The entire paper should use a two end aligned format.

Response 6:

Thank you very much for your suggestions and advice. It is your help that enables me to continually improve. It is your guidance that has enabled us to continually improve. The article has been revised to align both ends.

Recommendation 7: The title level is chaotic, for example, 1, 2, 3, etc. appear below 2.2.1.

Response 7:

Thank you very much for your suggestions and advice. It is your help that enables me to continually improve. It is your guidance that has propelled our continuous improvement. The title number of the article has been modified.

Recommendation 8: The introduction section should elaborate on the importance and significance of this research in detail.

Response 8:

Thank you very much for your suggestions and advice. It is your help that enables me to continually improve. The introduction section of the article has been rewritten to supplement and elaborate on the importance and significance of the research.

Recommendation 9: The first column of Table 2, Table 4 have two Year headers that need to be modified.

Response 9:

Thank you very much for your suggestions and advice. The corresponding issues have been modified.

Recommendation 10: The clarity of most figures is low and needs to be improved.

Response 10:

Thank you very much for your suggestions and advice. The corresponding issues have been modified.

Recommendation 11: The paper mentions the use of Tobit model to analyze the influencing factors of water resource utilization efficiency, but the application and analysis of formulas (7) and (8) in Tobit model are not used in the paper.

Response 11:

Thank you very much for your suggestions and advice. The article has added a fifth part, which supplements the application and analysis of Tobit model.

Recommendation 12: In the analysis of innovative points in the research content, there is no explanation of the innovative content, but rather a more accurate description of the research content is needed.

Response 12:

Thank you very much for your suggestions and advice. The article has supplemented the innovation in the abstract and introduction sections.

Recommendation 13: What is the basis for determining the evaluation index system?

Response 13:

Thank you very much for your suggestions and advice. In section 2.2.3 of the article, the selection criteria for indicators have been supplemented.

Recommendation 14: I highly recommend to write limitations of the study and recommendation for future study at the end of conclusion.

Response 14:

Thank you very much for your suggestions and advice. The conclusion section of the paper supplements the limitations of the research and the next steps of research.

Recommendation 15: The discussion on policies is not in-depth enough. It is recommended to include more policy analysis.

Response 15:

Thank you very much for your suggestions and advice. In the introduction section, an analysis and discussion of policies have been added

Attachments
Attachment
Submitted filename: Response to Reviewers.docx
Decision Letter - Akhtar Malik Muhammad, Editor

PONE-D-24-22328R1Examination of the Spatial-Temporal Variations in Terrestrial Water Reserves and Green efficiency of water resources in China's Three Northeastern ProvincesPLOS ONE

Dear Dr. yanying,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

==============================

Dear Authors,

Thank you for submitting the revised manuscript " Examination of the Spatial-Temporal Variations in Terrestrial Water Reserves and Green efficiency of water resources in China's Three Northeastern Provinces" to PLOS ONE.

A reviewer has not recommended your paper and suggests minor revisions. The reviewer’s comments need to be addressed to improve the paper quality. I go through the revised manuscript and reviewers’ comments. I suggest you should address the reviewer’s comments very seriously and resubmit a revised version.

Yours sincerely

Dr. Malik Muhammad Akhtar

Academic Editor

PLOS ONE

==============================

Please submit your revised manuscript by Jul 27 2025 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.
  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.
  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols. Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols.

We look forward to receiving your revised manuscript.

Kind regards,

Akhtar Malik Muhammad, PhD, Postdoc

Academic Editor

PLOS ONE

Journal Requirements:

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

Additional Editor Comments:

Dear Authors,

Thank you for submitting the revised manuscript " Examination of the Spatial-Temporal Variations in Terrestrial Water Reserves and Green efficiency of water resources in China's Three Northeastern Provinces" to PLOS ONE.

The reviewer-2 has not recommended your paper and suggests minor revisions. The reviewer’s comments need to be addressed to improve the paper quality. I go through the revised manuscript and reviewers’ comments. I suggest you should address the reviewer’s comments very seriously and resubmit a revised version.

Yours sincerely

Dr. Malik Muhammad Akhtar

Academic Editor

PLOS ONE

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: (No Response)

Reviewer #2: 1.I did not see any traces of modification in the file “Revised Manuscript with Track Changes”

2.There is no figure 8 in the uploaded picture.

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

**********

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.

Revision 2

Dear Reviewer and Editor:

We sincerely appreciate the constructive comments provided by the reviewers and editors regarding our manuscript. Your insights and guidance have served as a ladder for our continuous improvement, significantly enhancing the quality of our work. We deeply regret the issues identified in the manuscript and assure you that we will internalize the advice from experts in our future research and studies, striving for higher levels of scholarly achievement. Below is a detailed response addressing each comment.

Recommendation 1: I did not see any traces of modification in the file “Revised Manuscript with Track Changes”.

Response 1:

Thank you very much for your suggestion. There was an error in the annotation of my previous revised manuscript, so I have re uploaded the file 'Revised Manuscript with Track Changes'.

Recommendation 2: There is no figure 8 in the uploaded picture.

Response 2:

Thank you very much for your suggestion. I'm very sorry, due to my negligence, my image 8 was lost during uploading. I have uploaded it again.

Attachments
Attachment
Submitted filename: Response_to_Reviewers_auresp_2.docx
Decision Letter - Akhtar Malik Muhammad, Editor

Examination of the Spatial-Temporal Variations in Terrestrial Water Reserves and Green efficiency of water resources in China's Three Northeastern Provinces

PONE-D-24-22328R2

Dear Authors,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

An invoice will be generated when your article is formally accepted. Please note, if your institution has a publishing partnership with PLOS and your article meets the relevant criteria, all or part of your publication costs will be covered. Please make sure your user information is up-to-date by logging into Editorial Manager at Editorial Manager® and clicking the ‘Update My Information' link at the top of the page. If you have any questions relating to publication charges, please contact our Author Billing department directly at authorbilling@plos.org.

If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Akhtar Malik Muhammad, PhD, Postdoc

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Dear Authors,

Reviewers and Academic Editor are satisfied the modifications and revised version of the manuscript. The resolution of all figures should be improved to meet standard quality requirements.

The paper is accepted for the publication and further process.

Congratulations to authors.

Thanks to submit your research work to “PLOS ONE”

Dr. Malik Muhammad Akhtar

Academic Editor

PLOS ONE

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #2: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #2: I am satisfied with the author's modification, but there are still some problems with the pictures, such as Figure 4 and Figure 8, the clarity of pictures is low, resulting in some distortion of the text.

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #2: No

**********

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