PONE-D-25-21407Chiliadenus iphionoides: From Chemical Profiling to Anticancer, Antioxidant, α-amylase, and α-glycosidase activitiesPLOS ONE

Dear Dr. Bsharat,

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We look forward to receiving your revised manuscript.

Kind regards,

Uğur Cakilcioğlu, PhD

Academic Editor

PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: There are many studies on the antioxidative and anticancer effects of Chiliadenus iphionoides extracts. In this manuscript, the essential oils were isolated and their chemical content was determined, and the antimicrobial, antioxidative and anticancer effects of this oil were investigated. In this respect, the originality of the study is high. The study was well planned and appropriate methods were used. Plant names should be written in italics in the References section. In Fig. 3 and 4, A and B should be separate or B should be rearranged. Fig. 5 is not of good quality, it should be redrawn and rearranged. In Figs. 6 and 7, the fonts of the x and y axes should be the same.

Reviewer #2: Dear Editor; The attached article was checked. The manuscript contains interesting information about Chiliadenus iphionoides: From Chemical Profiling to Anticancer, Antioxidant, α-amylase, and α-glycosidase activities

I think that this article is well suited to your journal.

It is generally good work. The scientific and presentation level of the manuscript is high.

The title is understandable and in line with the text. The text is written in a descriptive and understandable language. The material and method are well described and adequately detailed Discussion and conclusion are interrelated.

What is the difference between the study and existing studies?

-add to the introduction; available in different on the wild edible plant studies

https://doi.org/10.1002/ardp.202300263

https://doi.org/10.1002/ardp.202300528

https://doi.org/10.1002/ardp.202400194

https://doi.org/10.1002/efd2.70021

In ms: Write the name of the authority to the end of the plant’s name.

- http://www.theplantlist.org/

Please, read the paper and correct them all.

What is the difference between the study and existing studies?

References were cross-checked.

-The paper should be edited according to the writing rules of the journal

Original manuscript. There are, however, a few minor changes required.

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Reviewer #1: No

Reviewer #2: No

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Chiliadenus iphionoides: From Chemical Profiling to Anticancer, Antioxidant, α-amylase, and α-glycosidase activities

PONE-D-25-21407R1

Dear Dr. Bsharat,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Uğur Cakilcioğlu, PhD

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: (No Response)

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: (No Response)

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Although there are many studies on the metabolite content and antioxidative antimicrobial effects of the extracts of various organs of Chiliadenus iphionoides in the manuscript, there is not enough research on the antioxidative, antimicrobial effects as well as anticancer effects of its essential oils. This situation increases the original value of the manuscript.

The authors have made the requested corrections. It is suitable for publication.

Reviewer #2: (No Response)

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean? ). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy .

Reviewer #1: Yes:  YASEMİN ÖZDENER KÖMPE

Reviewer #2: No

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