PONE-D-24-53986Rhus vulgaris Fruit-Mediated Silver Nanoparticles: Synthesis, Characterization, and Potential for Sun Protection, Antioxidant and Antibacterial ApplicationsPLOS ONE

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PLOS ONE

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Reviewers' comments:

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Comments to the Author

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The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Partly

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: No

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: No

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Dear Author, the manuscript entitled Rhus vulgaris Fruit-Mediated Silver Nanoparticles: Synthesis, Characterization, and

Potential for Sun Protection, Antioxidant and Antibacterial Applications was written good but still needs to be revised with the following points.

1. The abstract can be modified and rewritten.

2. The significance of R. vulgaris can be focused in the first part of the introduction.

3. The exploration of AgNPs can be effectively written in the introduction with specific biological properties.

4. Subheadings plant collection and preparation of plant extracts can be merged together and write in simplified manner.

5. Remove the subheadings of characterization part and merge together.

6. Why has the author done only one antioxidant activity?

7. The optimization part can be emphasized more with some more factors.

8. The discussion related with AgNPs towards skin related research can be discussed more.

9. Check for overall grammatical errors and sentence framing.

Reviewer #2: Authors presented “Rhus vulgaris Fruit-Mediated Silver Nanoparticles: Synthesis, Characterization, and Potential for Sun Protection, Antioxidant and Antibacterial Applications”, however, it requires more characterization part.

Authors should include and explain the below comments in the revised manuscript

1. Abstract should be rewrite, and should summarize the reason for the work, the most significant results, and the conclusions.

2. In introduction part, several categories of metallic NPs by green synthesis such as Au, Ag, Zno, Pd, TiO2, Cu, etc, have been reported with improved biomimetic attributes. Include these metal and metal oxide NPs with reference, Au (Phytosynthesis of gold nanoparticles using Caesalpinia pulcherrima (peacock flower) flower extract and evaluation of their antimicrobial activities) Ag (https://doi.org/10.1016/j.matpr.2020.10.048), ZnO and Cu (https://doi.org/10.1007/s43630-022-00224-0), Fe and Pd (DOI: 10.1039/C5CY00099H), etc.

3. Synthetic part: Why the authors used Rhus vulgaris Fruit extract only instead of other extract?

4. So many reports already published using the Rhus vulgaris Fruit extracts for the synthesis of AgNPs and biomedical application studies?

5. UV and IR spectral image should be clear, IR is not clear better to remove it

6. Characterization part: all figures including IR, TEM, should be clear

7. XRD for AgNPs required with clear hkl values (111, 200, 220, 311, 222 for AgNPs) and explain the hkl values

8. Single particle or HRTEM should be included with EDAX

9. Author should explain how the average size of AgNPs is 10 nm to 20 nm?

10. In addition, the lattice fringe spacings or D spacing hkl should be included

11. For antioxidant activity: author performed DPPH, what about ABTS, and NOx radical scavenging analyses? Compare and refer (https://doi.org/10.2147/IJN.S210517)

12. For antibacterial activity refer (DOI 10.1088/2053-1591/ad1357)

13. Compare the current work with previously published reports for AgNPs in separate table

14. Conclusions should be rewrite

15. Major English edition is required for whole manuscript

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Reviewer #1: No

Reviewer #2: No

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Rhus vulgaris Meikle Fruit-Mediated Silver Nanoparticles: Synthesis, Characterization, and Potential for Sun Protection, Antioxidant and Antibacterial Applications

PONE-D-24-53986R1

Dear Dr. Limenew Abate Worku

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Chellasamy Panneerselvam

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Comments from PLOS Editorial Office : We note that one or more reviewers has recommended that you cite specific previously published works. As always, we recommend that you please review and evaluate the requested works to determine whether they are relevant and should be cited. It is not a requirement to cite these works. We appreciate your attention to this request.

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The manuscript is written with adequate data related to the analysis carried out and is publishable.

Reviewer #2: The authors have adequately addressed all comments raised in a previous round of review and I feel that this manuscript is now acceptable for publication.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean? ). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy .

Reviewer #1: No

Reviewer #2: No

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