PONE-D-24-45627A modified rat model of 8 minutes asphyxial cardiac arrest and cardiopulmonary resuscitationPLOS ONE

Dear Dr. Ye,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

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ACADEMIC EDITOR: 

• Please address minor concerns raised by the reviewer 2

Please ensure that your decision is justified on PLOS ONE’s publication criteria  and not, for example, on novelty or perceived impact.

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Vadim Ten MD, PhD

Academic Editor

PLOS ONE

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Additional Editor Comments:

Dear Qingshan Ye.

Your manuscript has been reviewed by two independent reviewers and requires a minor revision to to be published.

Please, address minor concerns of the reviewer 2

Sincerely,

Vadim S. Ten MD, PhD

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: No

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: In this manuscript, the Authors endeavored to develop a refined protocol for a rat model of cardiac arrest and cardiopulmonary resuscitation. They argue that the described by them model “aligns closely with the pathophysiological changes observed in in-hospital cardiac arrest scenarios and effectively simulates a majority of clinical situations involving cardiac arrest”. The Authors now provide a very helpful narrative for step-by-step procedures and guidelines for standardization of experimental outcomes.

There are many merits to this manuscript. The study is well planned, experiments are well executed, and the document is expertly written. I concur with the Authors that longitudinal (14 days) evaluation of mortality and neurological outcomes is very helpful and provides peer researchers with a benchmark for sample size calculations at different experimental design time points. The limitations of the work are well described.

Below are several specific suggestions for improvements in data reporting and discussion of the literature:

[1] Moderate concern: As the proposed approach is not original, it would be very helpful to compare this study’s outcomes to the previous reports, both in the context of successful resuscitation and longer-term survival. The original methodological articles by HHL Hendrickx et al. (Resuscitation, 1984) and L Katz et al (JFRBM, 1995) are mentioned in Introduction but largely excluded from Discussion. To the best of this reviewer’s knowledge, Katz and colleagues reported much higher 72-h survival rates while using qualitatively similar procedure (why?). This and other studies deserve additional discussion.

[2] Moderate concern: The supplemental file is missing primary data for neurological outcomes presented in Figure 7C and 7D.

[3] Minor concern and suggestion for improvement: I would suggest including in the Supplemental files additional H&E staining images from more animals and more EM images. Such additional information will provide the specialists with a stronger basis for evaluating neurological damage in ACA brains. There is significant demand for such data for research and educational purposes.

Reviewer #2: The authors in this study established a stable 8 minutes asphyxial cardiac arrest and

cardiopulmonary resuscitation rat model and assessed the long-term prognosis,

providing a foundation for the researchers in the field into the mechanisms underlying cardiac

arrest and potential intervention strategies. This is an elegant study with extensive details on methodology that can serve as an experimental reference for the field. Further limitations of the study has been considered such as the use of only male but not the female rats. Overall, this is a very timely and well conducted study and is viewed as a significant resource for the field.

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Reviewer #1: No

Reviewer #2: No

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A modified rat model of 8 minutes asphyxial cardiac arrest and cardiopulmonary resuscitation

PONE-D-24-45627R1

Dear Dr. Qingshan Ye,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Vadim Ten

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The authors have addressed concerns raised during review of the previous version of this manuscript.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean? ). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy .

Reviewer #1: No

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