PONE-D-24-36200Unveiling Hub Genes and Biological Pathways: A Bioinformatics Analysis of Trauma-Induced Coagulopathy (TIC)PLOS ONE

Dear Dr. 张,

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ACADEMIC EDITOR: 

• fully revise English language of the manuscript
• Provide specific feedback from your evaluation of the manuscript

Our decision is justified on PLOS ONE’s publication criteria .

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Academic Editor

PLOS ONE

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 “2023 Yuncheng City Basic Research Program (Free Exploration Category) Projects

Item No� YCKJ-2023052”

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Additional Editor Comments:

Dear Authors

based on our review, the paper should undergo deep language revision before publication

Best regards

Academic Editor

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

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Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The paper, titled "Unveiling Hub Genes and Biological Pathways: A Bioinformatics Analysis of Trauma-Induced Coagulopathy (TIC)", explores the genetic basis of Trauma-Induced Coagulopathy (TIC), a severe condition resulting from traumatic injury. This study utilized bioinformatics techniques to analyze microarray datasets (GSE223245) from the GEO database, identifying 269 differentially expressed genes (DEGs), including 103 upregulated and 739 downregulated genes. GO and KEGG pathway analyses, along with Protein-Protein Interaction (PPI) network construction, identified 10 key hub genes closely linked to TIC. The diagnostic value of these genes was assessed using ROC analysis. The findings emphasize the potential role of these genes in TIC progression, though further experimental validation and clinical trials are necessary.

Major Concerns:

I have no major concerns with this manuscript. However, there are numerous English typos and grammatical errors that need to be addressed (see my comments in the uploaded PDF).

Minor Concerns

1. In the Introduction (page 10), the abbreviations "GO" and "KEGG" should be spelled out. This clarification should be made in the Introduction itself, not in the Functional Enrichment Analysis section.

2. The term "ROC" should be briefly explained in the Introduction.

3. Please include subheadings with appropriate numbering for the Methods section (page 11).

4. The introduction should also include any data in the literature about the role of certain known genes in the pathogenesis of TIC

5. On page 14, line 5, the sentence is unclear and appears to lack a verb. This should be revised for clarity.

6. Gene names should consistently be written in italics throughout the manuscript.

7. In the Conclusions section (page 20), you mention that your work is a groundbreaking discovery. However, given the limitations of the study (e.g., lack of clinical data and prospective cohorts), I recommend toning down this claim to provide a more balanced conclusion.

8. In Figures 3A, 3B, and 4A, the axes should be labeled.

9. The tools "CYTOHubba," "MCODE," and "CTD" should be briefly explained in the Introduction to provide context for readers.

10. There should be a table including the 10 DEGs identifies in this study and their effects of TIC

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Reviewer #1: No

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Attachments

Attachment

Submitted filename: PONE-D-24-36200.pdf

Unveiling Hub Genes and Biological Pathways: A Bioinformatics Analysis of Trauma-Induced Coagulopathy (TIC)

PONE-D-24-36200R1

Dear Dr. Zhang,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Monia Marchetti

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: