PONE-D-24-49902Time-Restricted Feeding Reduced Blood Pressure and Improved Cardiac Structure and Function by Regulating Both Circulating and Local Renin-Angiotensin Systems in Spontaneously Hypertensive Rat ModelPLOS ONE

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Additional Editor Comments:

ONE-PONE-D-24-49902

Time-Restricted Feeding Reduced Blood Pressure and Improved Cardiac Structure and Function by Regulating both Circulating and Local Renin-Angiotensin Systems in Spontaneously Hypertensive Rat Model

PLOS ONE

Dear Authors,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet journals publication criteria as it currently stands. Therefore, I recommend addressing all the important points raised by the reviewers in their report and addresses them point by point in the revised version of the manuscript for better understanding of the readers,

Kindly follow the proper guidelines for resubmitting the revised version along rebuttal letter for complete understanding of the reviewers during 2nd round of review process, before we proceed further in the publication process.

We look forward to receiving your revised manuscript.

Kind regards and best wishes

Sheryar Afzal, Ph.D.

Academic Editor

PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: In this paper Xin and co-authors determined to investigate whether time-restricted feeding (TRF) can reduce blood pressure (BP) and improve cardiac structure and function in spontaneously hypertensive rats (SHRs) by regulating the renin-angiotensin system (RAS). The outcomes of this study could be beneficial in terms of clinical practice and novel therapeutic strategies concerning hypertension.

1. Input some statistic figure for the hypertension as global health issue

2. IF stands for what?, abbreviation should be put at the first time when it is used.

3. In text, it mentioned, “RAS is a central component of the pathological process of hypertension and hypertension-induced cardiac remodeling and is a key therapeutic target”… Include some pathological evidence on RAS that related to hypertension after line 50.

For the laboratory animals, authors should present the animal sampling, or sample size calculation, inclusion and exclusion criteria, and or be included as supplementary files.

1. Please add the citation to support the sentences at line 214-216 regarding the SHI’S study of ADF intervention.

2. Please add future perspective of the study.

References are not properly written in the bibliography section as per journals criteria and style.

Reviewer #2: The study entitled "Time-Restricted Feeding Reduced Blood Pressure and Improved Cardiac Structure

and Function by Regulating Both Circulating and Local Renin-Angiotensin Systems in Spontaneously Hypertensive Rat Model" is interesting and well-designed. The authors tried to investigate the time-restricted feeding (TRF) effect on blood pressure reduction and associated improvement in cardiac anatomy and physiology in SHRs via RAAS regulation.

The study is interesting; however, the following points must be cleared for a better understanding of the study data and before proceeding for publication purposes.

1. the current statistical global update for hypertension and its associated comorbidities

2. All abbreviations must be defined first before being used in the text. Numerous terms are not explained properly while using abbreviations.

3. The mechanism of RAAS’s pathological association with hypertension and cardiac remodeling needs further explanation with the help of recent literature for a better understanding of the hypothesis and objectives of the study.

4. Did the authors observe any inclusion and exclusion criteria for the selection of animals? SHRs belong to the genetic model of hypertension and are age-dependent, competing with the criteria for hypertension.

5. If yes, please also provide complete details for animal grouping animals, acclimatization period, and environment

6. To improve the discussion further, compare the data findings with the recent literature on parallel lines to support the conclusions and study hypothesis.

7. A few studies are not properly cited in the discussion part, which creates ambiguity while understanding the study findings.

8. An example is "in lines 221 to 223, there is no supporting evidence from previous work on, if any, for the limited efficiency of TRF in prolonged intervention.

9. In conclusion, elaborate clearly that the study's outcome will assist in highlighting and identifying the therapeutic strategies for cardiac remodelling.

10. Some references are not properly cited. The journal editorial office must check the style of the references before final acceptance of the manuscript.

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Reviewer #1: Yes: Dr. Wu Yuan Seng

Reviewer #2: Yes: ALI ATTIQ

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Time-Restricted Feeding Reduced Blood Pressure and Improved Cardiac Structure and Function by Regulating Both Circulating and Local Renin-Angiotensin Systems in Spontaneously Hypertensive Rat Model

PONE-D-24-49902R1

Dear Dr. Ummi Nadira Daut

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Sheryar Afzal, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #2: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #2: Yes

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4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #2: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #2: (No Response)

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #2: Yes: ALI ATTIQ

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