PONE-D-24-45249Effects of Organic-inorganic Complex Fertilizer on the Growth and Physiological Characteristics of 'Qi-Nan' Agarwood from Aquilaria sinensis (Lour.)PLOS ONE

Dear Dr. Penglian,

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PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: No

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: No

Reviewer #2: Yes

Reviewer #3: No

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The manuscript gives a good impact on the effect of various organic and inorganic fertilizers on the valuable agarwood seedlings. Valuable data was collected and presented in tables and figures, however, it lacks some statistical significance tests. It is recommended to use and apply some statistical analysis to check the the difference between treatments. e.g.

Table 1 and Table 2 should be supported horizontally each row with one way ANOVA to check the difference between the treatment groups, and vertically with either repeated measure ANOVA and/or Pearson's correlation. the change in seedling height with time should be presented at least by (r) Pearson's correlation coefficient and two-tailed significance test. Moreover, the overall table could be tested with two way repeated measure ANOVA (Factor1= treatment, Factor2= Time)

In addition, some minor corrections in the manuscript, including punctuations and other typing errors, should be revised.

Figure 1. caption line 181, the word Treat. would be expressed as "treatment." would be better

The figure legend isnot clear; if possible, clarify.

Line 106 in the materials and Methods part, "The average seedling height was 17.60±0.33..." please clarify which was used with the average (±SD) or average (±SE)

Reviewer #2: YES, manuscript technically sound, and do the data support the conclusions. Additon the authors provide the findings in their manuscript. Author applied statistical analysis which parallel finding conclusion. More language uses is understandable to reader.

Reviewer #3: There are some concerns about present manuscript as follow: the aquired data is belonged to one year study and so is not statistically more reliable. Moreover, There is noy any other external factor which studied beside fertilizer tretment.

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Reviewer #1: Yes:  Abdelghafar M. Abu-Elsaoud

Reviewer #2: Yes:  ARIF ALI

Reviewer #3: No

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Attachments

Attachment

Submitted filename: PONE-D-24-45249_reviewer.pdf

Effects of Organic-inorganic Complex Fertilizer  on the Growth and Physiological Characteristics of 'Qi-Nan' Agarwood from Aquilaria sinensis (Lour.)

PONE-D-24-45249R1

Dear Dr. Penglian,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Mojtaba Kordrostami, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #2: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #2: Yes

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4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #2: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #2: All the above sections include the English language; statistically, literature is written clearly and correctly. I would recommend proceeding further.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean? ). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy .

Reviewer #2: No

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