PONE-D-24-52009Analysis of the release pattern of floral aroma coyunmponents of Rhus chinensis based on HS-SPME-GC-MS techniquePLOS ONE

Dear Dr. Wang,

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The manuscript submitted by Wang et al. mainly dealt with analysis of the release pattern of floral aroma components of Rhus chinensis based on HS-SPME-GC-MS technique. The article is interesting and applicable. However, some problems or errors should be revised or explained according to the following suggestions.

title: coyunmponents should be components

Abstract

Please add one sentence to introduce the background of this study.

line 105, 80 celcius degree might be very high, why did you select such a high temperature? I worried about the volatiles being degraded.

line 106, please tell us what's the type of the fiber of SPME ?

line 120, relative retention time of each peak, aiming to determine the diverse volatile components in the aroma of R. chinensis flowers corresponding to each peak. why not use RI or kovatts index.

Table 1, RT should be revised into RI

Reviewer #2: The title of this article is "Analysis of the release pattern of floral aroma components of Rhus chinensis based on HS-SPME-GC-MS technique. Headspace-solid phase microextraction was used to identify the essential components of the floral aroma during the budding, blooming, and withering of the flowering stages of Rhus chinensis.. Overall, the article is very interesting, the experiments are properly designed, the data is sufficient, the results are impressive and meaningful. The revision advice is as below:

1，Please add the significance of the study aim in the abstract.

2，Lines 29 and 30: "reaching 3.60μg/g at the full bloom stage 29 and only 2.40μg/g after the bloom stage." Please check for errors, as there is a discrepancy between the current abstract and the results. It is advisable to correct.

3，The introduction is too concise. More literature regarding the research scope is recommended, rather than discussing the scent of honey.

4，The authors report that the floral aroma compounds were identified through each peak's retention time and peak area. However, the total ion current (TIC) chromatograms for the determination of floral aroma compounds are not presented in the results. It is advisable to submit the relevant supplementary materials.

5，Since Table 2 only lists compound classes without specific identifications. This complicates result interpretation. I suggest including a table with the 84 compounds in the Supplementary Materials.

6，Tables should use three-wire tables.

7, Acknowledgments： Project support numbers should be added.

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Reviewer #1: No

Reviewer #2: Yes:  Shu Wang

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Analysis of the release pattern of floral aroma components of Rhus chinensis based on HS-SPME-GC-MS technique

PONE-D-24-52009R1

Dear Dr. Wang%,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Mozaniel Santana de Oliveira, Ph.D

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #2: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #2: Yes

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4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #2: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #2: The author has answered my questions and revised the paper carefully. I have no additional suggestions at this time.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean? ). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy .

Reviewer #2: No

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