PONE-D-24-18894Highly Sensitive Molecular Assay Based on Identical Multi-Repeat Sequence (IMRS) Algorithm for the Detection of Trichomonas vaginalis InfectionPLOS ONE

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Reviewers' comments:

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Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: The manuscript describes a sensitive isothermal amplification technique targeting repetitive sequences on an important pathogen. However, it should have been tested on more clinical samples including vaginal swabs, genital secretions, urine and should also be evaluated against microscopy and culture. Discussion should include few more studies especially those demonstrating a good sensitivity and specificity. Since authors from a commercial entity , the conflict of interest statement should clearly mention the same.

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Reviewer #1: No

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Highly Sensitive Molecular Assay Based on Identical Multi-Repeat Sequence (IMRS) Algorithm for the Detection of Trichomonas vaginalis Infection

PONE-D-24-18894R1

Dear Dr. Gitaka,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Adriana Calderaro

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Thanks to have responded to the reviewers' comments.

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