PONE-D-24-18303Stem cell factor restrains endoplasmic reticulum stress-associated apoptosis through c-kit receptor activation of JAK2/STAT3 axis in hippocampal neuronal cellsPLOS ONE

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Academic Editor

PLOS ONE

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 [This work was supported by grants from the "13th Five-Year" Science and Technology Project of Education Department of Jilin Province (JJKH20200456KJ)].  

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Please provide uncropped western blotting images as supplementary files. 

Reviewers' comments:

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Comments to the Author

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Reviewer #1: Yes

Reviewer #2: Partly

Reviewer #3: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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Reviewer #1: Yes

Reviewer #2: No

Reviewer #3: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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5. Review Comments to the Author

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Reviewer #1: The article is written and the hypothesis is work well. Authors indicated that the findings provides valuable insights into the molecular mechanisms underlying the pathogenesis of AD and explores novel therapeutic targets for both sporadic and familial AD. It would be better if they explain shortly how manage to explore novel therapotic targets.

Reviewer #2: I uploaded my comments as word file.

As it is written in the manuscript, the sample size and statistical analysis seem appropriate. The authors have made some data available as supporting material, including RT-PCR results, and ethics and animal documents. However, these documents are all in Chinese, so I cannot check them. I did not see any uncropped blot membranes, which could be a concern. As I am not a native English speaker, I cannot evaluate the language of the manuscript.

Reviewer #3: The study titled "Stem cell factor restrains endoplasmic reticulum stress-associated apoptosis through c-kit receptor activation of JAK2/STAT3 axis in hippocampal neuronal cells" is a well-designed article that clearly explains the purpose and study methods to the reader-scientists. The work can be accepted without requiring revision. However, it is recommended that authors make a few simple suggestions at the proof-reading stage, if appropriate for the Editor.

The author should add introduction part how to manage these inhibitors as AD theraputics and emphasis its importance more.

Please correct in Material and Methods part test names fully for instance “3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT) Test” or “5-Bromo-2'-deoxyuridine (BrdU) Test”

Figures microscope photos does not look clear, a clearer image can be added for all figures

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Reviewer #1: Yes: Prof. G. OZHAN

Reviewer #2: No

Reviewer #3: No

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Attachments

Attachment

Submitted filename: review plos one.docx

Attachment

Submitted filename: Comments PLOSONE-PONE-D-24-18303.docx

Stem cell factor restrains endoplasmic reticulum stress-associated apoptosis through c-kit receptor activation of JAK2/STAT3 axis in hippocampal neuronal cells

PONE-D-24-18303R1

Dear Dr. SHEN,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Abhishek Kumar Singh, Ph.D.

Academic Editor

PLOS ONE