PONE-D-24-18374Dimethyl Itaconate Modulates EEG Activity, Oxidative stress and Inflammation in Neonatal Seizure-Affected Rats: Implications for Cognitive Function and Anxiety BehaviorPLOS ONE

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https://bmcimmunol.biomedcentral.com/articles/10.1186/s12865-021-00463-3?

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: The manuscript evaluates the protective effect of DMI on hippocampus EEG, behavioural and biochemical parameters in hypoxia-induced seizure on neonatal period. Some major revision needs to be done before publication, as mentioned below:

1. The title is too long. The authors can brief it such as above. Because inflammation and oxidative stress are very common.

2. In the abstract: add the volume of vehicle, the age of rats, the delay time for experiments on adult rats and say pre-treatment is executed.

3. How long does hypoxia effects last? Because you work on 10 day neonatal rats and record EEG in adult rat. It is interesting and surprising for me.

4. The discussion also be written too long (about 7 pages!). Please brief it and focus on the principal parts "EEG". For example, explain why beta, alpha and delta waves significantly decreased in hypoxia group, while delta power increased? What is the relationship between hypoxia and these powers? The authors wrote "during period of hypoxia ", during period is ok or after period?

5. Insert the EEG power image for all groups.

6. Are you see seizure after HINS period? If see, for how long?

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Reviewer #1: No

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The protective effect of DMI on hippocampus EEG, behavioral and biochemical parameters in hypoxia-induced seizure on neonatal period

PONE-D-24-18374R1

Dear Dr. Ghotbeddin,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Xiaona Wang, Ph.D

Academic Editor

PLOS ONE