Glutathione transferase P1 is modified by palmitate

Vanessa Marensi; Megan C. Yap; Yuhuan Ji; Cheng Lin; Luc G. Berthiaume; Elaine M. Leslie

doi:10.1371/journal.pone.0308500

Peer Review History

Original SubmissionMay 28, 2024
10 Jul 2024 Decision Letter - Mohd Akbar Bhat, Editor PONE-D-24-21632Glutathione transferase P1 is modified by palmitatePLOS ONE Dear Dr. Leslie, Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process. Please submit your revised manuscript by Aug 24 2024 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file. 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In your cover letter, please note whether your blot/gel image data are in Supporting Information or posted at a public data repository, provide the repository URL if relevant, and provide specific details as to which raw blot/gel images, if any, are not available. Email us at plosone@plos.org if you have any questions. [Note: HTML markup is below. Please do not edit.] Reviewers' comments: Reviewer's Responses to Questions Comments to the Author 1. Is the manuscript technically sound, and do the data support the conclusions? The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. Reviewer #1: Yes Reviewer #2: Yes ******** 2. Has the statistical analysis been performed appropriately and rigorously? Reviewer #1: Yes Reviewer #2: Yes ****** 3. 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Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here. Reviewer #1: Yes Reviewer #2: No ****** 5. Review Comments to the Author Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters) Reviewer #1: Only this sentnce "GSTP1 is the most prevalent and widely distributed GST, in humans GSTP1 is found in most cell types and tissues, with the exception of healthy adult hepatocytes (4, 5)" may require modification for improved comprehension Reviewer #2: R1: • enjoyable and modern topic. • The title is suitable. • All data are presented in intense and poring way. • Authors must read previous papers to re-write this paper as it deserve. Abstract: -better to divide abstract (summary of your study) to sub titles include: -background -aim or objective of the study -methods -results -conclusions of results -this give complete idea about the research -enough to use glutathione transferase P1, palmitic acid as key-words INTRODUCTION: -authors can define the abbreviation which mentioned for one time, and list the abbreviations that repeated for many time. RESULTS: -research performed in highly precise methods, but more than requested data were mentioned. please re-write this section depend on the following guide to represent your idea. to describe this section please summarize the methods as below in subtitle: - experiential animal. - sample collection or extraction. - sample preparation - materials(use scientific name and avoid the trade name and marks) - principle(use standard principle). - avoid repetition of information. -please do not use trade mark and names of companies, persons or labs to prove your data source. write in scientific way. -avoid poring details that describe your way and behavior to perform research. -Marensi is one of the authors not source of figure. RESULTS: -the author results were describe the principle of study sample or methods for sample preparation. result must contain only the result of authors experiment. -please do not discuss or justifies the results in this section. DISCUSSION: -good discussion of the results. this paragraph to be completed need prove of previous or recent studies that accordance with or different from author results. -the discussion must be limited to results only. -please compare these result with previous researches, and so on ..... for all results. -better to discuss directly the results only in prief, precise and scientific language. -avoid repetition of data. -by the end of discussion you can add limitations and recommendation in points. ****** 6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files. If you choose “no”, your identity will remain anonymous but your review may still be made public. Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy. Reviewer #1: Yes: Samuel Acquah Reviewer #2: No ******** [NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.] While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step. Attachments Attachment Submitted filename: PONE-D-24-21632_reviewer.pdf https://doi.org/10.1371/journal.pone.0308500.r001
Revision 1
22 Jul 2024 Author Response Included in the cover letter which has better formatting. July 21st, 2024 Dear Dr. Mohd Akbar Bhat Thank you for your email of July 10th, 2024 inviting us to submit a revised version of our manuscript PONE-D-24-21632 entitled “Glutathione Transferase P1 is modified by palmitate”. We appreciate the reviews provided. As requested, we have responded to the specific comments (bold and italicized) of the editor and reviewers point by point below, and where necessary, revised the manuscript accordingly. Editor comments: 1. When submitting your revision, we need you to address these additional requirements.Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The manuscript has now been formatted according to the PLOS ONE style templates. 2. We note that you have provided funding information that is not currently declared in your Funding Statement. However, funding information should not appear in the Acknowledgments section or other areas of your manuscript. We will only publish funding information present in the Funding Statement section of the online submission form. Please remove any funding-related text from the manuscript and let us know how you would like to update your Funding Statement. We have removed all funding statements from the manuscript body. We do not need to amend the online submission form, it is complete as is. 3. We note that you have included the phrase “data not shown” in your manuscript. Unfortunately, this does not meet our data sharing requirements. PLOS does not permit references to inaccessible data. We require that authors provide all relevant data within the paper, Supporting Information files, or in an acceptable, public repository. Please add a citation to support this phrase or upload the data that corresponds with these findings to a stable repository (such as Figshare or Dryad) and provide and URLs, DOIs, or accession numbers that may be used to access these data. Or, if the data are not a core part of the research being presented in your study, we ask that you remove the phrase that refers to these data. The data are only supportive and not core to the manuscript so we have removed the phrase that referred to the data. 4. Please include captions for your Supporting Information files at the end of your manuscript, and update any in-text citations to match accordingly. We have now included a caption for our single supporting information file that contains original uncropped and unadjusted images underlying all blot or gel results. We have not referred to this file in the text. 5. PLOS ONE now requires that authors provide the original uncropped and unadjusted images underlying all blot or gel results reported in a submission’s figures or Supporting Information files. All of our blot/gel image data for figures contained in the manuscript are in Supporting Information Fig S1. Reviewer #1 comments: This reviewer had only one suggestion: The sentence "GSTP1 is the most prevalent and widely distributed GST, in humans GSTP1 is found in most cell types and tissues, with the exception of healthy adult hepatocytes (4, 5)" may require modification for improved comprehension. We have now modified this sentence to: GSTP1 is a prevalent and widely distributed GST, found in most human cell types and tissues, with the exception of healthy adult hepatocytes. Reviewer #2 comments: This reviewer had suggestions about the presentation of the manuscript with no criticisms of the data or research itself. The following positive comments were made: • enjoyable and modern topic. • The title is suitable. • All data are presented in intense and poring way. Changes requested: 1)The reviewer asked us to divide the abstract into subtitles (Background, Aim, Methods, Results, Conclusions). This does not seem to be a formatting requirement of PLOS ONE and in our opinion the abstract flows better without subheadings. It is our preference to leave the abstract as it is. We will comply with the reviewer’s request if the editor prefers. 2) Enough to use glutathione transferase P1, palmitic acid as key-words. We think it’s important to have the different types of palmitoylation (N-, S- and O-palmitoylation) as keywords so we have kept these (rather than replacing them with palmitic acid). We have deleted glutathione. 3) INTRODUCTION: -authors can define the abbreviation which mentioned for one time, and list the abbreviations that repeated for many time. The abbreviation MCF-7 has now been defined as Michigan Cancer Foundation-7 in the text and the abbreviations list. All other abbreviations have been defined with first use. The reviewer highlighted the last paragraph of the introduction and stated “results” as a comment. Our interpretation of this comment was that the reviewer thinks this section belongs in the results. We have deleted this paragraph and replaced it with the objectives of the study. 4) Methods-research performed in highly precise methods, but more than requested data were mentioned. please re-write this section depend on the following guide to represent your idea. To describe this section please summarize the methods as below in subtitle: - experiential animal. - sample collection or extraction. - sample preparation - materials(use scientific name and avoid the trade name and marks) - principle(use standard principle). - avoid repetition of information. We have gone through our methods section and removed repetitive information but we have not subdivided under the suggested headings because we feel they are too general or do not apply (for example we don’t have experimental animals in our study). -please do not use trade mark and names of companies, persons or labs to prove your data source. Write in scientific way. It is necessary to provide names of companies so that people can order comparable reagents to complete similar studies. If there is a PLOS ONE policy that we have missed in the instructions to authors please let us know and we will comply. -avoid poring details that describe your way and behavior to perform research. It is not clear what this means. 5) RESULTS: -the author results were describe the principle of study sample or methods for sample preparation. result must contain only the result of authors experiment. -please do not discuss or justifies the results in this section. We have gone through our results section and deleted any unnecessary discussion. 6) DISCUSSION: a) Second paragraph good discussion of the results. This paragraph to be completed need prove of previous or recent studies that accordance with or different from author results. -the discussion must be limited to results only. Appropriate references to previous studies are included. b)-please compare these result with previous researches, and so on ….. for all results. -better to discuss directly the results only in prief, precise and scientific language. -avoid repetition of data. -by the end of discussion you can add limitations and recommendation in points. Where possible we have shortened the discussion and major limitations are now described in two sentences within our concluding paragraph. Thank you for consideration of our revised manuscript, which we hope you will find acceptable for publication in PLOS ONE. Yours sincerely, Elaine M. Leslie, Ph.D. https://doi.org/10.1371/journal.pone.0308500.r002
25 Jul 2024 Decision Letter - Mohd Akbar Bhat, Editor Glutathione transferase P1 is modified by palmitate PONE-D-24-21632R1 Dear Dr. Leslie, We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements. Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication. An invoice will be generated when your article is formally accepted. Please note, if your institution has a publishing partnership with PLOS and your article meets the relevant criteria, all or part of your publication costs will be covered. Please make sure your user information is up-to-date by logging into Editorial Manager at Editorial Manager® and clicking the ‘Update My Information' link at the top of the page. If you have any questions relating to publication charges, please contact our Author Billing department directly at authorbilling@plos.org. If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org. Kind regards, Mohd Akbar Bhat Academic Editor PLOS ONE Additional Editor Comments (optional): The authors have amended the manuscript as per the reviewer comments. Reviewers' comments: https://doi.org/10.1371/journal.pone.0308500.r003
Formally Accepted
30 Jul 2024 Acceptance Letter - Mohd Akbar Bhat, Editor PONE-D-24-21632R1 PLOS ONE Dear Dr. Leslie, I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now being handed over to our production team. At this stage, our production department will prepare your paper for publication. This includes ensuring the following: * All references, tables, and figures are properly cited * All relevant supporting information is included in the manuscript submission, * There are no issues that prevent the paper from being properly typeset If revisions are needed, the production department will contact you directly to resolve them. If no revisions are needed, you will receive an email when the publication date has been set. At this time, we do not offer pre-publication proofs to authors during production of the accepted work. Please keep in mind that we are working through a large volume of accepted articles, so please give us a few weeks to review your paper and let you know the next and final steps. Lastly, if your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org. If we can help with anything else, please email us at customercare@plos.org. Thank you for submitting your work to PLOS ONE and supporting open access. Kind regards, PLOS ONE Editorial Office Staff on behalf of Dr. Mohd Akbar Bhat Academic Editor PLOS ONE https://doi.org/10.1371/journal.pone.0308500.r004

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