PONE-D-24-22445Effect of storage plans on the oxidative stability and nutritional quality of selected commercially available seed oilsPLOS ONE

Dear Dr. Ijaz,

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Mojtaba Kordrostami, Ph.D.

Academic Editor

PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Partly

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Dear Authors

This research article aimed to investigate the effect of storage conditions on the oxidative stability of vegetable oils. Generally, the manuscript (MS) is fairly written and organized. MS contains some useful information. The methodology and data collection correspond to the objectives. However, to make this MS meet the standard of the PLOS ONE Journal, some more information and revisions of the MS are required.

1. The Title may be revised as the nutritional compositions of the oils were only determined in raw materials before storage. This would suggest “Nutritional quality of selected seed oils and effect of storage conditions on their oxidative stability”

2. The abstract should be revised. Some important information must be covered and reflect the main findings according to the objectives.

-Lines 21, the seed oils used in this study should be specified, what are they?

-Short information about methodology must be included as the abstract should be stand-alone.

-Line 23, should be mentioned the values of all seed oils.

- Reorganize the writing, the results in the abstract should be written in the same order as in the results section.

Lines 34-35 should be revised, please see the details and comments provided in the conclusion section.

3. Introduction section:

3.1 Lines 76-82 should be deleted. It has already been mentioned in the methodology.

3.2 The literature review is insufficient. A background information and literature review on the effect of improper environmental conditions on oil quality and chemical compositions reported by previous studies are required.

4. Materials and methods

4.1 Lines 145-151, please revise and detail the storage conditions. The room temperature should be in range, not a single temperature. How to get the oils to explode to light and absent to light. What kind and intensity of light? How to control temperature during storage at high temp.

5. Results and discussion

-More discussion on section FA composition, tocopherols, TPC, and TFC are required. The values of these parameters obtained in this study could be both higher and lower than previous studies or even similar to other studies, so authors need to discuss all sides. More citations are required.

- From the values of FFA, PV, and PAV, authors should also discuss the standard values of these parameters, as long as these values obtained do not exceed the standard values, these would be accepted.

-In Figures 1, 2, and 3, the error bars or superscripts should be inserted to indicate statistical differences.

6. The conclusion should be revised. In lines 339-343, the authors did not determine or compare the oxidative stability values among the oil types, so authors should not conclude whether SBO is the most unstable, instead, authors should focus on answering the main objectives. If authors would like to conclude or provide this information, authors should statistically compare these values among the oil types.

Reviewer #2: The authors did lots of works, and the results were meaningful. some suggestions were presented:

1) Total Phenolic Contents: one time was not enough to fully extract the phenolic compounds by 80% methanol and hexane. three times might be better. in addation, the concentration of Folin was missing. detailed information maybe important for other researchers to repeat your tests and data.

2)Tocopherol and Tocotrienol Contents: the determination conditions by HPLC should be presented clearly. If possible, please provided the HPLC chromatogram.

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Reviewer #1: No

Reviewer #2: Yes: Liyou Zheng

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Nutritional quality of selected commercially available seed oils and effect of storage conditions on their oxidative stability

PONE-D-24-22445R1

Dear Dr. Ijaz,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Mojtaba Kordrostami, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The title, abstract, methodology, results, and discussion have now been improved. Some references were updated. According to these changes, the authors appear to have adequately amended the comments made by the reviewer. Thank you for your effort in amending the manuscript.

Reviewer #2: (No Response)

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: Yes: Liyou Zheng

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