PONE-D-24-05753The expression of MIR125B transcripts and bone phenotypes in Mir125b2 deficient micePLOS ONE

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Reviewer #1: This paper investigates the role of Mir125b2 in the bone phenotype of 3 months old mice. Using CRISPR/Ca9system the authors delete Mir125b2 globally and convincingly show the deletion with mRNA. . KO male and female mice have the same weight than respective WT control and grow normally. Deletion of Mir125b2 does not cause any bone phenotype as indicated by the analysis of the tibua by micro-CT and histomorphometry in male mice The analysis is detailed and presented appropriately.

Minor issues:

The paper would benefit y the review of an English professional service.

The absence of the bone phenotype should be confirmed in another bone such as vertebra or femur.

Overall, this paper clearly conveys the message and does not have any major issues.

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Reviewer #1: No

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The expression of MIR125B transcripts and bone phenotypes in Mir125b2-deficient mice

PONE-D-24-05753R1

Dear Dr. Ogasawara,

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Gary S. Stein

Academic Editor

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