PONE-D-24-07704Unveiling the gut bacteriome diversity and signature of the Bangladesh national fish hilsa (Tenualosa ilisha)PLOS ONE

Dear Dr. Islam,

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Bijay Kumar Behera, Ph.D.

Academic Editor

PLOS ONE

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https://doi.org/10.1007/s11033-023-08965-6

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“This research was partially supported with funds from ‘BSMRAU Physical Facility and Research Capacity Strengthening Project’ under the Ministry of Education, People’s Republic of Bangladesh for funding this research, Grant No.: BSMRAU/01/2021. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.”

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Additional Editor Comments (if provided):

Please find the two reviewers comments, and my decision is a minor revision of the manuscript.

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The manuscript entitled, Unveiling the gut bacteriome diversity and signature of the Bangladesh national fish hilsa (Tenualosa ilisha) (Manuscript Number: PONE-D-24-07704) by Kawser and co-worker is well written in the manuscript. However, minor modifications are required. Please see the comments.

1. Key Words are to be Keywords

2. The abstract section is well written

3. Line no 147: used instead of utilized

4. The result section is well written

5. Discussion should be more discussed with the latest literature

6. Image quality should to improved

Reviewer #2: The manuscript titled “Unveiling the gut bacteriome diversity and signature of the Bangladesh national fish hilsa (Tenualosa ilisha)” Manuscript Number: PONE-D-24-07704 is well written.

However, refer to the comments below to rectify the manuscript-

1. The title should include “Diversity and distribution” of gut bacteriome.

2. Line No. 45 The Keywords should be Tenualosa ilisha, Gut microbiome, Metagenomics, 16S rRNA, Probiotic properties.

3. In the Introduction, we introduce Tenualosa Ilisha and provide Nutritional information about the Hilsa fish. We also provide the IUCN Status of the particular fish species in view of the conservation effort.

4. Line No. 117 Write the word ‘used’ instead of utilized.

5. The Methodology part of the manuscript is well-written.

6. The discussion part of the manuscript is also well written with distinct functioning of the Bacteriome found.

7. Figure quality is very poor; it should be at least 300 dpi resolution.

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Reviewer #1: Yes: Ajaya Kumar Rout

Reviewer #2: Yes: Sushree Swati Rout

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Unveiling the gut bacteriome diversity and distribution in the national fish hilsa (Tenualosa ilisha) of Bangladesh

PONE-D-24-07704R1

Dear Dr. Islam,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Bijay Kumar Behera, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: