PONE-D-23-38632Effects of eicosapentaneoic acid on innate immune responses in an Atlantic salmon kidney cell line in vitroPLOS ONE

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AAM (Straive) 28 Nov 2023: IRB for Animal Not Stated

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Summary

The article discusses the field of immunometabolism, focusing on the interaction between nutrients, energy metabolism, and the immune system in both health and disease. It emphasizes the limited understanding of the interplay between feed and immunity in fish, particularly in the context of dietary changes in Atlantic salmon. The traditional dietary sources of essential fatty acids, such as fish oil and fish meal, are being substituted by plant oils, impacting the levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in salmon diets. The study explores the effects of different cellular levels of EPA on the transcriptional responses of salmon cells to the viral mimic poly I:C, revealing a potential link between EPA levels and the modulation of immune responses. The article is well-written, scientifically rigorous, and contributes valuable insights to the field of fish immunometabolism.

General Comments

I acknowledge the limitations of generalizing findings from Atlantic salmon cells to other fish species or different populations of Atlantic salmon. Explicitly discuss how the observed changes might impact the overall well-being and performance of fish.

• The article primarily focuses on EPA, but it would be valuable to explore potential interactions with docosahexaenoic acid (DHA) since fish oil traditionally contains both EPA and DHA (Lines 68-69).

• The reliance on in vitro cell culture experiments is noted. Consider addressing the limitations of the cell culture model and discussing how in vivo studies could complement these findings (Lines 110-112, 368-370).

• The study assesses short-term effects after one week. Consider discussing potential long-term effects or the sustainability of observed changes over extended periods (Lines 114-124).

Recommendation

I recommend this manuscript for publication in Plos One. The study opens avenues for further research on the role of EPA in antiviral immunity in live fish and highlights the scarcity of similar studies in fish.

Reviewer #2: Dear authors,

Congratulations and you have done a good job. I have critically gone through the manuscript entitled “Effects of eicosapentaneoic acid on innate immune responses in an Atlantic salmon kidney cell line in vitro”. However, you need to incorporate the revision and answer the comments that are stated below before it is published.

1. Abbreviation should be expanded while writhing for the first time.

2. L1 write the scientific name of Atlantic salmon.

3. L28 and L30 write the full form of VEGF and PPAR.

4. L42 write the full form of RLR signalling.

5. Broken line analysis may use to know the exact EPA level rather than writing it as 200 uM EPA. It may be less or more than 200 uM EPA

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Reviewer #1: No

Reviewer #2: Yes: Gyandeep Gupta

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Effects of eicosapentaneoic acid on innate immune responses in an Atlantic salmon kidney cell line in vitro

PONE-D-23-38632R1

Dear Dr. Gjøen,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Amit Ranjan, Ph.D.

Academic Editor

PLOS ONE

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #3: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #3: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #3: Yes

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4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #3: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #3: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The authors have addressed all the comments; therefore, the manuscript can be accepted for publication.

Reviewer #3: This study investigated various cellular EPA levels modulate the antiviral transcriptional responses in salmon cells during stimulation with poly I:C, and the results suggested that innate antiviral responses are heavily influenced by the fatty acid profile of salmonid cells and constitute another example of the strong linkage between general metabolic pathways and inflammatory responses.

The manuscript had been well prepared and could be considered for publication.

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