Peer Review History

Original SubmissionMarch 21, 2024
Decision Letter - Yitagesu Habtu Aweke, Editor

PONE-D-24-05509Healthcare Providers’ Perceptions on Post Abortion Intrauterine Contraception: A qualitative study in central UgandaPLOS ONE

Dear Dr. Kayiga, Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by Jul 25 2024 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file. 

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.
  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.
  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

We look forward to receiving your revised manuscript.

Kind regards,

Yitagesu Habtu Aweke, Ph.D

Academic Editor

PLOS ONE

Journal Requirements:

When submitting your revision, we need you to address these additional requirements.

1. Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The PLOS ONE style templates can be found at 

https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and 

https://journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf

2. Thank you for stating the following financial disclosure: 

"I received part of the funding from Kalorinska Institutet in partnership with Makerere University for the data collection and analysis but not in towards the publication and dissemination. I would be grateful if I can be availed a full publication waiver towards my publication.

I have in the past reviewed peer articles for free for this highly reputable journal and would be grateful if my request for a publication waiver is given your kind consideration."

Please state what role the funders took in the study.  If the funders had no role, please state: ""The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript."" 

If this statement is not correct you must amend it as needed. 

Please include this amended Role of Funder statement in your cover letter; we will change the online submission form on your behalf.

3. When completing the data availability statement of the submission form, you indicated that you will make your data available on acceptance. We strongly recommend all authors decide on a data sharing plan before acceptance, as the process can be lengthy and hold up publication timelines. Please note that, though access restrictions are acceptable now, your entire data will need to be made freely accessible if your manuscript is accepted for publication. This policy applies to all data except where public deposition would breach compliance with the protocol approved by your research ethics board. If you are unable to adhere to our open data policy, please kindly revise your statement to explain your reasoning and we will seek the editor's input on an exemption. Please be assured that, once you have provided your new statement, the assessment of your exemption will not hold up the peer review process.

4. Please include captions for your Supporting Information files at the end of your manuscript, and update any in-text citations to match accordingly. Please see our Supporting Information guidelines for more information: http://journals.plos.org/plosone/s/supporting-information.

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Partly

Reviewer #3: Yes

**********

2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: No

Reviewer #3: No

**********

3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: No

Reviewer #3: Yes

**********

4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

**********

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Summary of the Research

Despite access to post abortion intrauterine contraception, the uptake of Intrauterine devices (IUDs) in Uganda is below four percent. Whether the perceptions of healthcare providers towards IUDs have a role in the provision of post abortion IUDs remains unclear. The aim of study to explore the perceptions of healthcare providers towards post abortion IUDs after medical management of first trimester incomplete abortions. Methods: Between 1st August 2022 and 30th September 2022, forty-five in-depth interviews were conducted among healthcare providers of different cadres in sixteen public health facilities in central Uganda. The interviews were primarily to help us understand the perceptions of healthcare providers towards IUDs. All interviews were audio-recorded and transcribed verbatim. Themes were identified using inductive content analysis. From the content analysis of the interviews, three themes emerged. Theme one covered health facility related barriers such as healthcare providers’ and health system challenges towards provision of post abortion IUDs. The second theme was around the challenges in reaching and counselling women to use the post abortion IUDs. The third theme covered the motivating factors and participants’ views on how to scale up IUD uptake and provision within postabortion care in Uganda. Even with the availability of the necessary materials and equipment required for post abortion IUDs, and improved logistics such as timing of insertion to overcome some of the barriers faced by patients, service delivery hinges on the healthcare providers’ perceptions. Measures to address healthcare providers’ knowledge gaps and IUD skill sets should be enhanced. Healthcare providers’ misconceptions need to be addressed with provision of evidence-based information to enable correct information to patients. There is a need to address health system barriers like patient overload, stock-outs of equipment and materials for IUD insertion, and privacy. Provision of on-job refresher trainings, community sensitization on IUDs, financial incentives, and support supervision are key motivators that can be utilized in supporting healthcare providers towards post abortion IUD provision.

Areas for improvement

Title:

Good title.

Abstract:

Clear and good abstract.

Key words: limits the words to the most important, 5-6 words is enough.

Order of the authors:

Mention all authors of this review please.

Make sure that all necessary information for all authors are available in this document.

Introduction Section:

In one sentence explain if maternal death is associated with not using birth spacing methods.

The authors should revise introduction section for grammar issues and language to improve readability.

Join paragraphs that discussing the same concepts.

The authors should indicate what they wanted the readers to understand.

Overall

Good introduction but the authors need to arrange introduction section.

Material and Methods:

Study design,

Clear.

Setting,

Clear.

Participants recruiting,

Clear.

Inclusion and exclusion criteria,

Clear.

Quality Assessment,

The authors should explain the how the maintained validity of data.

Data collection and analysis,

Clear, however topic guild should be explained in detail with the questions.

Ethical consideration,

Clear.

Results:

The authors should revise the language and grammar to improve readability.

Discussion:

The authors should make clear for the readers about the themes that they were discussing.

The authors should discuss each themes from different angle and in detail.

The authors should revise the language and grammar to improve readability.

References:

The authors should revise all references according to the guidelines provided.

Overall: Good manuscript, all the best.

Reviewer #2: This study describes the perceptions of healthcare providers in Central Uganda on post-abortion intrauterine contraception. However, the inclusion of only 45 interviewees does not adequately represent the broader situation in Central Uganda. The study employs inductive content analysis, but this method also has some shortcomings, particularly in terms of replicability, bias impact, and theoretical depth. The subjectivity of the researchers may affect which data are coded and how they are coded, thereby influencing the objectivity and reliability of the research results. Researchers may tend to select data that supports their anticipated hypotheses while ignoring or undervaluing data that does not fit their expectations. This could lead to the research results being biased or incomplete.

Reviewer #3: The manuscript is entitled: "Healthcare Providers’ Perceptions on Post-Abortion Intrauterine Contraception: A Qualitative Study in Central Uganda'".

The authors explored the healthcare providers' perceived barriers and facilitators to post-abortion IUDs after medical management of first-trimester incomplete abortions in Central Uganda.

General Comment: The research topic is relevant to the field and the authors thoroughly explored the research topic and demonstrated a deep understanding of the subject matter. However, during a review of the manuscript, the following areas were identified as a major concern

1. The authors clearly described the gap in low uptake of IUDs in Uganda, however, they failed to define the evidence gap. Specifically, evidence gap on the health providers' perceptions of post-abortion IUD. What others presented on the topic needs to be well presented. So, What were the gaps in the literature?

2. The title of the manuscript describes healthcare providers' Perceptions, however, what is presented in the result, discussion and even in the conclusion part is entirely about the barriers and facilitators to post-abortion IUD uptake?? It lacks coherence. The ideas should flow logically from one section to the next, with clear transitions. Each section should build upon the previous one, guiding the reader through the manuscript.

3. The reviewer can not say the methodology is sound and replicable with confidence

3.1. Despite having a clear study design, the authors exhaustively discussed the philosophical perspective that aligns with this study. "A qualitative interview study" is not specific. Which kind of qualitative study design was employed to answer the research question???

3.2. The authors assigned two independent researchers whose task was to ensure rigor in the study according to the Lincoln–Guba criteria. That's excellent. However, how was trustworthiness (rigor) ensured in this study? Specifically, what techniques were used to establish credibility, confirmability, dependability, and transferability?

Additionally, the following minor revisions/comments were identified:

1. Abstract

1.1. Methods: what was analyzed and the type of analysis conducted?

1.2. Results: Content analysis appeared in the result part. wouldn't it be repeating the methods in the result section?

2. Results

2.1. the title of Table 1: why Baseline characteristics?

2.2. Table 2: is not self-explanatory and is very long

3. Discussion: Unnecessary details were observed in this section, therefore, the authors need to be more focused while discussing only the main findings. Moreover, very long sentences need to modified again.

**********

6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: Zalikha Khamis Al-Marzouqi

Reviewer #2: No

Reviewer #3: Yes: Andamlak Gizaw Alamdo

**********

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.

Attachments
Attachment
Submitted filename: feedback PONE.docx
Revision 1

COLLEGE OF HEALTH SCIENCES

SCHOOL OF MEDICINE

DEPARTMENT OF OBSTETRICS AND GYNAECOLOGY

15th July 2024

RESPONSE TO REVIEWS COMMENTS:

Object: PONE-D-24-05509: “HEALTHCARE PROVIDERS’ PERCEPTIONS ON POST ABORTION INTRAUTERINE CONTRACEPTION: A QUALITATIVE STUDY IN CENTRAL UGANDA”

With great pleasure, I’m thankful for your comments towards our manuscript. In response to the reviewers’ comments sent to us on 11th June 2024, we have revised the manuscript accordingly.

Comment Response to Comment Page No. and Line

Journal Requirements:

When submitting your revision, we need you to address these additional requirements.

1. Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The PLOSONE style templates can be found at

https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and

https://journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf

2. Thank you for stating the following financial disclosure:

“ I received part of the funding from Kalorinska Institutet in partnership with Makerere University for the datacollection and analysis but not in towards the publication and dissemination. I would be grateful if I can be availed a full publication waiver towards my publication.

I have in the past reviewed peer articles for free for this highly reputable journal and would be grateful if my request for a publication waiver is given your kind consideration."

Please state what role the funders took in the study. If the funders had no role, please state: ""The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.""

If this statement is not correct you must amend it as needed.

Please include this amended Role of Funder statement in your cover letter; we will change the online submission form on your behalf.

3. When completing the data availability statement of the submission form, you indicated that you will make your data available on acceptance. We strongly recommend all authors decide on a data sharing plan before acceptance, as the process can be lengthy and hold up publication timelines. Please note that, though access restrictions are acceptable now, your entire data will need to be made freely accessible if your manuscript is accepted for publication. This policy applies to all data except where public deposition would breach compliance with the protocol approved by your research ethics board. If you are unable to adhere to our open data policy, please kindly revise your statement to explain your reasoning and we will seek the editor's input on an exemption. Please be assured that, once you have provided your new statement, the assessment of your exemption will not hold up the peer review process.

4. Please include captions for your Supporting Information files at the end of your manuscript, and update any in-text citations to match accordingly. Please see our Supporting Information guidelines for more information: http://journals.plos.org/plosone/s/supporting-information.

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Partly

Reviewer #3: Yes

2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: No

Reviewer #3: No

3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: No

Reviewer #3: Yes

4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your reviewas an attachment if it exceeds 20,000 characters)

Reviewer #1: Summary of the Research

Despite access to post abortion intrauterine contraception, the uptake of Intrauterine devices (IUDs) in Uganda is below four percent. Whether the perceptions of healthcare providers towards IUDs have a role in the provision of post abortion IUDs remains unclear. The aim of study to explore the perceptions of healthcare providers towards post abortion IUDs after medical management of first trimester incomplete abortions. Methods: Between 1st August 2022 and 30th September 2022, forty-five in-depth interviews were conducted among healthcare providers of different cadres in sixteen public health facilities in central Uganda. The interviews were primarily to help us understand the perceptions of healthcare providers towards IUDs. All interviews were audio-recorded and transcribed verbatim. Themes were identified using inductive content analysis. From the content analysis of the interviews, three themes emerged. Theme one covered health facility related barriers such as healthcare providers’ and health system challenges towards provision of post abortion IUDs. The second theme was around the challenges in reaching and counselling women to use the post abortion IUDs. The third theme covered the motivating factors and participants’ views on how to scale up IUD uptake and provision within postabortion care in Uganda. Even with the availability of the necessary materials and equipment required for post abortion IUDs, and improved logistics such as timing of insertion to overcome some of the barriers faced by patients, service delivery hinges on the healthcare providers’ perceptions. Measures to address healthcare providers’ knowledge gaps and IUD skill sets should be enhanced. Healthcare providers’ misconceptions need to be addressed with provision of evidence-based information to enable correct information to patients. There is a need to address health system barriers like patient overload, stock-outs of equipment and materials for IUD insertion, and privacy. Provision of on-job refresher trainings, community sensitization on IUDs, financial incentives, and support supervision are key motivators that can be utilized in supporting healthcare providers towards post abortion IUD provision.

Areas for improvement

Title:

Good title.

Abstract:

Clear and good abstract.

Key words: limits the words to the most important, 5-6 words is enough.

Order of the authors:

Mention all authors of ffthis review please.

Make sure that all necessary information for all authors are available in this document.

Introduction Section:

In one sentence explain if maternal death is associated with not using birth spacing methods.

The authors should revise introduction section for grammar issues and language to improve readability.

Join paragraphs that discussing the same concepts.

The authors should indicate what they wanted the readers to understand.

Overall

Good introduction but the authors need to arrange introduction section.

Material and Methods:

Study design,

Clear.

Setting,

Clear.

Participants recruiting,

Clear.

Inclusion and exclusion criteria,

Clear.

Quality Assessment,

The authors should explain the how the maintained validity of data.

Data collection and analysis,

Clear, however topic guild should be explained in detail with the questions.

Ethical consideration,

Clear.

Results:

The authors should revise the language and grammar to improve readability.

Discussion:

The authors should make clear for the readers about the themes that they were discussing.

The authors should discuss each themes from different angle and in detail.

The authors should revise the language and grammar to improve readability.

References:

The authors should revise all references according to the guidelines provided.

Overall: Good manuscript, all the best.

Reviewer #2: This study describes the perceptions of healthcare providers in Central Uganda on post-abortion intrauterine contraception. However, the inclusion of only 45 interviewees does not adequately represent the broader situation in Central Uganda.

The study employs inductive content analysis, but this method also has some shortcomings, particularly in terms of replicability, bias impact, and theoretical depth. The subjectivity of the researchers may affect which data are coded and how they are coded, thereby influencing the objectivity and reliability of the research results. Researchers may tend to select data that supports their anticipated hypotheses while ignoring or undervaluing data that does not fit their expectations. This could lead to the research results being biased or incomplete.

Reviewer #3: The manuscript is entitled: "Healthcare Providers’ Perceptions on Post-Abortion Intrauterine Contraception: A Qualitative Study in Central Uganda'".

The authors explored the healthcare providers' perceived barriers and facilitators to post-abortion IUDs after medical management of first-trimester incomplete abortions in Central Uganda.

General Comment: The research topic is relevant to the field and the authors thoroughly explored the research topic and demonstrated a deep understanding of the subject matter. However, during a review of the manuscript, the following areas were identified as a major concern

1. The authors clearly described the gap in low uptake of IUDs in Uganda, however, they failed to define the evidence gap. Specifically, evidence gap on the health providers' perceptions of post-abortion IUD. What others presented on the topic needs to be well presented. So, What were the gaps in the literature?

2. The title of the manuscript describes healthcare providers' Perceptions, however, what is presented in the result, discussion and even in the conclusion part is entirely about the barriers and facilitators to post-abortion IUD uptake?? It lacks coherence. The ideas should flow logically from one section to the next, with clear transitions. Each section should build upon the previous one, guiding the reader through the manuscript.

3. The reviewer can not say the methodology is sound and replicable with confidence

3.1. Despite having a clear study design, the authors exhaustively discussed the philosophical perspective that aligns with this study. "A qualitative interview study" is not specific. Which kind of qualitative study design was employed to answer the research question???

3.2. The authors assigned two independent researchers whose task was to ensure rigor in the study according to the Lincoln–Guba criteria. That's excellent. However, how was trustworthiness (rigor) ensured in this study? Specifically, what techniques were used to establish credibility, confirmability, dependability, and transferability?

Additionally, the following minor revisions/comments were identified:

1. Abstract

1.1. Methods: what was analyzed and the type of analysis conducted?

1.2. Results: Content analysis appeared in the result part. wouldn't it be repeating the methods in the result section?

2. Results

2.1. the title of Table 1: why Baseline characteristics?

2.2. Table 2: is not self-explanatory and is very long

3. Discussion: Unnecessary details were observed in this section, therefore, the authors need to be more focused while discussing only the main findings. Moreover, very long sentences need to modified again.

6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: Zalikha Khamis Al-Marzouqi

Reviewer #2: No

Reviewer #3: Yes: Andamlak Gizaw Alamdo

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.

The manuscript has been revised in accordance to the PLOSONE style templates as recommended.

"The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript."

All the necessary data and materials underlying the findings described have been provided as part of this manuscript. The authors agree to the free data sharing plan of the materials. In case any more data or materials are needed, they are readily accessible in the Department of Obstetrics and Gynecology library, Makerere University College of Health Sciences, Kampala, Uganda.

The manuscript has been revised in accordance to the PLOSONE style templates. We have added a caption for the supporting information as recommended.

Thanks to the reviewers for considering the manuscript to be technically sound.

We appreciate the reviewers for the comments in this regard. We have availed the revised manuscript, the approved protocol, ethical approval forms from the School of Medicine, Makerere University Ethic Review, and the Uganda National Committee of Science and Technology, informed consent and interview guide for the in-depth interviews. We have also attached the descriptive characteristics of the study sites without exposing the identity of the participants. The authors agree to the data sharing plan.

Thanks to the reviewers for considering the manuscript worthy in this regard.

The key words used in the revised manuscript are now five. They are as follows; First-trimester abortion, Intrauterine-contraception, Perceptions, Healthcare

Attachments
Attachment
Submitted filename: Response to reviewer comments_finale.docx
Decision Letter - Yitagesu Habtu Aweke, Editor

Healthcare Providers’ Perceptions on Post Abortion Intrauterine Contraception: A qualitative study in central Uganda

PONE-D-24-05509R1

Dear Dr. Herbert Kayiga,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

An invoice will be generated when your article is formally accepted. Please note, if your institution has a publishing partnership with PLOS and your article meets the relevant criteria, all or part of your publication costs will be covered. Please make sure your user information is up-to-date by logging into Editorial Manager at Editorial Manager® and clicking the ‘Update My Information' link at the top of the page. If you have any questions relating to publication charges, please contact our Author Billing department directly at authorbilling@plos.org.

If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Yitagesu Habtu Aweke, Ph.D

Academic Editor

PLOS ONE

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #3: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #3: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #3: N/A

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #3: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #3: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #3: (No Response)

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #3: Yes: Andamlak Gizaw Alamdo

**********

Formally Accepted
Acceptance Letter - Yitagesu Habtu Aweke, Editor

PONE-D-24-05509R1

PLOS ONE

Dear Dr. Kayiga,

I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now being handed over to our production team.

At this stage, our production department will prepare your paper for publication. This includes ensuring the following:

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Thank you for submitting your work to PLOS ONE and supporting open access.

Kind regards,

PLOS ONE Editorial Office Staff

on behalf of

PhD Candidate Yitagesu Habtu Aweke

Academic Editor

PLOS ONE

Open letter on the publication of peer review reports

PLOS recognizes the benefits of transparency in the peer review process. Therefore, we enable the publication of all of the content of peer review and author responses alongside final, published articles. Reviewers remain anonymous, unless they choose to reveal their names.

We encourage other journals to join us in this initiative. We hope that our action inspires the community, including researchers, research funders, and research institutions, to recognize the benefits of published peer review reports for all parts of the research system.

Learn more at ASAPbio .