Insecticidal activity of Ageratina adenophora (Asteraceae) extract against Limax maximus (Mollusca, Limacidae) at different developmental stages and its chemical constituent analysis

Haojun Li; Runa Zhao; Yingna Pan; Hui Tian; Wenlong Chen

doi:10.1371/journal.pone.0298668

Peer Review History

Original SubmissionOctober 17, 2023
13 Nov 2023 Decision Letter - Vivekanandhan Perumal, Editor PONE-D-23-33974Insecticidal activity of Ageratina adenophora (Asteraceae) extract against Limax maximus (Mollusca, Limacidae) at different developmental stages and its chemical constituent analysisPLOS ONE Dear Dr. li, Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process. Please submit your revised manuscript by Dec 28 2023 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file. 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The PLOS ONE style templates can be found at https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and https://journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf 2. In your Data Availability statement, you have not specified where the minimal data set underlying the results described in your manuscript can be found. PLOS defines a study's minimal data set as the underlying data used to reach the conclusions drawn in the manuscript and any additional data required to replicate the reported study findings in their entirety. All PLOS journals require that the minimal data set be made fully available. For more information about our data policy, please see http://journals.plos.org/plosone/s/data-availability. 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We will update your Data Availability statement to reflect the information you provide in your cover letter. 3. We note that you have stated that you will provide repository information for your data at acceptance. Should your manuscript be accepted for publication, we will hold it until you provide the relevant accession numbers or DOIs necessary to access your data. If you wish to make changes to your Data Availability statement, please describe these changes in your cover letter and we will update your Data Availability statement to reflect the information you provide. 4. Please include your full ethics statement in the ‘Methods’ section of your manuscript file. In your statement, please include the full name of the IRB or ethics committee who approved or waived your study, as well as whether or not you obtained informed written or verbal consent. If consent was waived for your study, please include this information in your statement as well. Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice. Additional Editor Comments: Comments to the authors The manuscript title is “Insecticidal activity of Ageratina adenophora (Asteraceae) extract against Limax maximus (Mollusca, Limacidae) at different developmental stages and its chemical constituent analysis” by Li et al. After carefully reviewing, I can see some merit in this research. The current form is not up to the scientific level, so please carefully fix the following errors. The introduction part is poorly written, and several details are missing in the current form. Please add information about current control methods and their disadvantages, then write about alternative biological control methods. Only then will the reader understand clearly; otherwise, they will be confused. Line 60: typo error Line 66: Why did you dry plant materials at 60°C for 24 hours? because at 60 oC, most of the volatile compounds will degrade, so please check it. Line 66: Please add the made and made of the mechanical grinder. Why have you selected only distilled water for extraction? What about other solvents? Figures 1–9: Please change them and add high-quality images. [Note: HTML markup is below. Please do not edit.] Reviewers' comments: Reviewer's Responses to Questions Comments to the Author 1. Is the manuscript technically sound, and do the data support the conclusions? The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. Reviewer #1: Yes Reviewer #2: Yes ******** 2. Has the statistical analysis been performed appropriately and rigorously? Reviewer #1: Yes Reviewer #2: Yes ****** 3. Have the authors made all data underlying the findings in their manuscript fully available? The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified. Reviewer #1: No Reviewer #2: Yes ****** 4. Is the manuscript presented in an intelligible fashion and written in standard English? PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here. Reviewer #1: Yes Reviewer #2: Yes ****** 5. Review Comments to the Author Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters) Reviewer #1: 1. Good manuscript but can be improved further. 2.Improve on your data presentation to be more concise, and include more interesting points in your discussions. 3. Language can be improved as well. 4. Please see more on the comment attachment. Reviewer #2: The manuscript Insecticidal activity of Ageratina adenophora (Asteraceae) extract against Limax maximus (Mollusca, Limacidae) at different developmental stages and its chemical constituent analysis, has been written well but needs minor revisions. ****** 6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files. If you choose “no”, your identity will remain anonymous but your review may still be made public. Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy. Reviewer #1: Yes: Rajiv Ravi Reviewer #2: No ******** [NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.] While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step. Attachments Attachment Submitted filename: Plosone Review.docx Attachment Submitted filename: PONE-D-23-33974_reviewed.pdf https://doi.org/10.1371/journal.pone.0298668.r001
Revision 1
4 Jan 2024 Author Response Dear Editor and Reviewers, Thanks very much for taking your time to review this manuscript. I really appreciate all your comments and suggestions! Please find my itemized responses in below and my revisions/corrections in the re-submitted files SUGGESTIONS FROM EDITOR The introduction part is poorly written, and several details are missing in the current form. Please add information about current control methods and their disadvantages, then write about alternative biological control methods. Only then will the reader understand clearly; otherwise, they will be confused. Line 60: typo error Line 66: Why did you dry plant materials at 60°C for 24 hours? because at 60 oC, most of the volatile compounds will degrade, so please check it. Line 66: Please add the made and made of the mechanical grinder. Why have you selected only distilled water for extraction? What about other solvents? Figures 1–9: Please change them and add high-quality images. Academic Editor: 1. I have perfected the introduction of the manuscript, adding the current control methods and shortcomings, and explaining the alternative biological control methods, and why plant extracts are selected for control. 2. Line 60: typo error (Modified) 3. Line 66: Why did you dry plant materials at 60°C for 24 hours? I chose to dry at 60℃ for 24 hours because I referred to someone else's method. Most plant extracts need to be dried before they are prepared. Plant extracts mainly play a role in secondary metabolites, which can only be extracted by heating or even evaporation, and the extracts in this study still have obvious toxic effect on Limax maximus. 4. Line 66: Please add the made and made of the mechanical grinder. (Added) 5. Why have you selected only distilled water for extraction? What about other solvents? First of all, distilled water is selected because its influencing factors are small and suitable as a control, and pre-experiments have been done to confirm that it has no toxic effect on Limax maximus, and other solvents may have a better effect. This is a point that needs to be improved in our research in the future. Thank you very much for your suggestion. 6. Figures 1–9: Please change them and add high-quality images. (Changed) Reviewer #1: Abstract 1. Please include the LC50 value specifically for 24 hours and 48 hours The LC50 value in our study was calculated by virulence test. 24 h and 48 h were the treatment time set by the extract with LC50 concentration, so there was only one LC50 value. 2. In my opinion, there should be two values of the LC dose, might be lower for longer exposure time LC50 is a semi-lethal concentration, the specific meaning is that in the acute toxicity test of animals, the concentration of poison that makes half of the animals killed is generally only one value, and the virulence test is carried out at the same time, the difference is that the concentration of dilution is different. Introduction 1. Expand and explain line 45-48 more the use of this plant to control mollusk species At present, the example of using Ageratina adenophora to control molluscs is still very rare. the purpose of using Ageratina adenophora extract on Limax maximus in our study is to explore whether this extract has killing effect on Limax maximus. in order to develop a new botanical pesticide. 2. What other studies have used this plant for controlling the mollusk species. What is the specific chemical component novel from this plant which has been used for biocidal activity? No other studies have used this plant to control molluscs, and the special chemical components we have identified are flavonoids, saponins and tannins, all of which have bactericidal activity. however, we do not rule out the fact that other undetected chemical components also have killing activity, which is also a direction of our future research. Materials and Methods 1. Line 56-69, please include pictures for slug and plants with its morphological identification about their species. Verification of species information required for both slug and plant We have added pictures of Limax maximus and Ageratina adenophora to the manuscript, because we have given detailed species names, and this study does not belong to the taxonomic category, and the corresponding species can be found well according to the species names. Results 1. Please include a summary table for flavonoids, saponins and tannins, to enable readers for quick look at the content compositions. We have attached a summary table to make the data more intuitive. 2. Please include some interesting pictures showing the growth analysis, size differences between control and treatment. Line 280-286 etc We added a growth comparison chart to the Supporting Information Captions. Discussions 1. Line 382-387, add more discussions on previous studies using plant extracts in relation to molluscicidal activity We added some discussions about the use of plant extracts to control molluscs. 2. Discuss the advantage of your extracts compare to chemical usage. Please include some literatures about the degradation profile on plant extracts compared to chemical treatments. Can your natural plant extracts be degraded fast in actual environment compared to chemical usage?? Chemical agents are often accompanied by 3R problems, and there are few articles about the degradation of plant extracts compared with chemicals. At present, the use of plant extracts as a new chemical is still in the experimental stage. Field experiments and degradation analysis have not yet been carried out. In order to really apply it to practical control, we will speed up our research to ensure compliance with the principles of the use of pesticides. Thank you for your suggestions. 3. Explain more line 436-445 on chemicals harm to human and environment. What is your plant extracts applicability in industrial approach. We have explained the harm of chemicals to human beings and the environment, as well as the convenience of our extracts in factory production. 4. What can be the degradation profile, shelf life for your extracts, how long can your extract will be effective. Example, can your extract still be effective after keeping in solution preps for more than 1-3 months. The degradation of our extract has not been studied yet, which is an important indicator for us to apply it to field experiments. Our extract can still be effective after 1-3 months of preservation, which has been explained in the manuscript. Reviewer #2: We modified some sentence patterns and phrases according to the requirements of the reviewer#2. We would like also to thank you for allowing us to resubmit a revised copy of the manuscript. We hope that the revised manuscript is accepted for publication in the Plos One. Attachments Attachment Submitted filename: Response to Reviewers.docx https://doi.org/10.1371/journal.pone.0298668.r002
25 Jan 2024 Decision Letter - Vivekanandhan Perumal, Editor PONE-D-23-33974R1Insecticidal activity of Ageratina adenophora (Asteraceae) extract against Limax maximus (Mollusca, Limacidae) at different developmental stages and its chemical constituent analysisPLOS ONE Dear Dr. li, Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process. Please submit your revised manuscript by Mar 10 2024 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file. 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Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols. Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols. We look forward to receiving your revised manuscript. Kind regards, Vivekanandhan Perumal, Ph.D Academic Editor PLOS ONE Journal Requirements: Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice. Additional Editor Comments: Editor comments After revising the manuscript, it now meets scientific standards. However, the quality of the images is poor. I kindly request the authors to provide high-quality images for Figures 1 and need scale bar, 3, 4, 5, 6, and 7. The high quality of images helps strengthen your paper. I hope you understand. [Note: HTML markup is below. Please do not edit.] Reviewers' comments: Reviewer's Responses to Questions Comments to the Author 1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation. Reviewer #1: All comments have been addressed Reviewer #2: All comments have been addressed ******** 2. Is the manuscript technically sound, and do the data support the conclusions? The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. Reviewer #1: Yes Reviewer #2: Yes ****** 3. Has the statistical analysis been performed appropriately and rigorously? Reviewer #1: Yes Reviewer #2: Yes ****** 4. Have the authors made all data underlying the findings in their manuscript fully available? The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified. Reviewer #1: Yes Reviewer #2: (No Response) ****** 5. Is the manuscript presented in an intelligible fashion and written in standard English? PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here. Reviewer #1: Yes Reviewer #2: Yes ****** 6. Review Comments to the Author Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters) Reviewer #1: Comments has been accepted and changes is significantly good for acceptance. The manuscript is good to be accepted as, it shows a preliminary findings on the plant extracts and potentially can be novel discovery for plant extracts. Furthermore, the graphs and data sets supports the findings. Reviewer #2: The manuscript Insecticidal activity of Ageratina adenophora (Asteraceae) extract against Limax maximus (Mollusca, Limacidae) at different developmental stages and its chemical constituent analysis has been modified well, now it may be Accepted for publication in plos one ****** 7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files. If you choose “no”, your identity will remain anonymous but your review may still be made public. Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy. Reviewer #1: No Reviewer #2: No ******** [NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.] While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step. Attachments Attachment Submitted filename: Comments@2024.docx https://doi.org/10.1371/journal.pone.0298668.r003
Revision 2
26 Jan 2024 Author Response Dear Additional Editor, Thanks very much for taking your time to review this manuscript. I really appreciate all your comments and suggestions! Please find my itemized responses in below and my revisions/corrections in the re-submitted files SUGGESTIONS FROM EDITOR We have uploaded our figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool to ensure that our pictures meet the requirements. We added the scale bar to Fig 1 and the clarity of Fig 3, 4, 5, 6 and 7 to make it look clearer. Finally, we checked our reference list to make sure that our reference list were complete and accurate and not rejected. We would like also to thank you for allowing us to resubmit a revised copy of the manuscript. We hope that the revised manuscript is accepted for publication in the Plos One. Attachments Attachment Submitted filename: Response to Reviewers.docx https://doi.org/10.1371/journal.pone.0298668.r004
30 Jan 2024 Decision Letter - Vivekanandhan Perumal, Editor Insecticidal activity of Ageratina adenophora (Asteraceae) extract against Limax maximus (Mollusca, Limacidae) at different developmental stages and its chemical constituent analysis PONE-D-23-33974R2 Dear Dr. li, We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements. Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication. An invoice for payment will follow shortly after the formal acceptance. To ensure an efficient process, please log into Editorial Manager at http://www.editorialmanager.com/pone/, click the 'Update My Information' link at the top of the page, and double check that your user information is up-to-date. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org. If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org. Kind regards, Vivekanandhan Perumal, Ph.D Academic Editor PLOS ONE Additional Editor Comments (optional): The authors have responded to my comments, and now the manuscript meets scientific standards. Therefore, I recommend this manuscript for publication. Reviewers' comments: https://doi.org/10.1371/journal.pone.0298668.r005
Formally Accepted
14 Feb 2024 Acceptance Letter - Vivekanandhan Perumal, Editor PONE-D-23-33974R2 PLOS ONE Dear Dr. Li, I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now being handed over to our production team. At this stage, our production department will prepare your paper for publication. This includes ensuring the following: * All references, tables, and figures are properly cited * All relevant supporting information is included in the manuscript submission, * There are no issues that prevent the paper from being properly typeset If revisions are needed, the production department will contact you directly to resolve them. If no revisions are needed, you will receive an email when the publication date has been set. At this time, we do not offer pre-publication proofs to authors during production of the accepted work. Please keep in mind that we are working through a large volume of accepted articles, so please give us a few weeks to review your paper and let you know the next and final steps. Lastly, if your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org. If we can help with anything else, please email us at customercare@plos.org. Thank you for submitting your work to PLOS ONE and supporting open access. Kind regards, PLOS ONE Editorial Office Staff on behalf of Dr. Vivekanandhan Perumal Academic Editor PLOS ONE https://doi.org/10.1371/journal.pone.0298668.r006

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